Gene:
Gene Symbol:
MYO18B
Gene Name:
myosin XVIIIB
Original Clone ID:
ccsbBroadEn_12841
Keyword:
None
Species:
Homo sapiens
Type:
cDNA
Vector Name:
pENTR223
Format:
FUSION
Source:
The ORFeome Collaboration
Description:
Original GenBank Accession: BC150626.1
Comments:
None
Mutation / Discrepancy:
No / No
Publications:
None
Authors:
Harvard Medical School
Dana-Farber Cancer Institute
Broad Institute of Harvard and Massachusetts Institute of Technology (MIT)
David E Hill
David E Root
The ORFeome Collaboration
Dana-Farber Cancer Institute
Broad Institute of Harvard and Massachusetts Institute of Technology (MIT)
David E Hill
David E Root
The ORFeome Collaboration
HsCD00516507
Price:
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AVAILABLE
No restriction
Special MTA: None
Special MTA: None
ATGGCCATCTCATCACGCCTCGCCCTGTGGGAGCAGAAGATTCGGGAAGAGGACAAGAGC
CCTCCACCATCCTCGCCCCCTCCTCTTTTCTCTGTCATCCCAGGGGGCTTCATTAAGCAA
CTGGTCCGGGGGACTGAAAAAGAGGCCAAGGAAGCGAGACAGAGGAAGCAGTTAGCTGTC
GCCTCTCCAGAACGAGAGATCCCAGAAATTTCCATCAGCCAACCCAACAGCAAGTCCAGC
AGTGGCACCAGATCTGGAAGCCAGCAGATCTCTCAGGACGACCAGTCAAGCTCTCCTGGG
AGCTCAGACATTCTGGGCAAGGAGAGCGAGGGGTCCCGCAGCCCCGACCCTGAGCAGATG
ACAAGCATCAATGGTGAGAAGGCCCAGGAGCTGGGCTCCAGTGCGACACCAACCAAAAAG
ACTGTCCCCTTCAAGAGGGGCGTGAGGAGGGGTGATGTGTTGTTGATGGTGGCCAAGCTG
GACCCGGACTCAGCCAAGCCAGAGAAGACTCATCCCCATGACGCCCCCCCTTGCAAGACC
TCTCCCCCCGCCACAGATACTGGAAAGGAAAAGAAAGGGGAGACCTCTAGGACTCCTTGT
GGCTCCCAGGCCAGCACCGAGATCTTGGCCCCGAAAGCTGAGAAGACCCGGACTGGGGGT
CTTGGGGACCCAGGCCAAGGAACTGTGGCACTGAAAAAAGGCGAGGAGGGTCAAAGCATA
GTGGGGAAGGGGCTTGGGACCCCCAAGACCACAGAGCTGAAAGAGGCTGAGCCCCAGGGC
AAAGACAGGCAGGGGACCAGGCCCCAAGCCCAAGGGCCCGGCGAGGGGGTGCGACCAGGG
AAAGCAGAGAAGGAGGGAGCAGAGCCCACAAACACGGTGGAAAAGGGGAATGTCTCTAAG
GACGTAGGGAGTGAAGGGAAGCACGTAAGGCCCCAAATCCCTGGGAGAAAGTGGGGAGGT
TTCCTGGGAAGAAGGAGTAAGTGGGACGGTCCCCAGAATAAGAAGGACAAAGAAGGGGTG
CTCTTAAGTAAGGCAGAGAAGACAGGTGAGCCTCAGACCCAGATGGAGAAGACAAGCCAA
GTGCAGGGCGAGTTGGGGGACGATCTGAGAATGGGGGAGAAAGCAGGTGAGCTTCGGAGC
ACGACTGGGAAGGCAGGTGAGTCCTGGGATAAGAAGGAAAAGATGGGGCAACCCCAGGGT
AAGTCCGGGAACGCAGGTGAAGCTCGGAGTCAGACAGAGAAGGGCTGTGAAGCCCCAAAG
GAGGTGAGCACAATGGTGGAGTCGCCAGCAGCTCCTGGGAAGGGAGGCTGGCCAGGAAGC
CGTGGGCAGGAAGCAGAGGAGCCCTGCTCAAGAGCAGGTGATGGGGCTGGTGCCCTGGAG
ACAGAGCTGGAAGGACCCAGCCAGCCTGCTCTGGAGAAGGATGCAGAAAGGCCTCGGATA
CGGAAGGAGAACCAAGACGGGCCAGCCCCGCAGGAGGAGGGCAAAGGAGGCCAGAGCAGA
GACTCAGACCAGGCTCCTGAGGACAGATGGTATGAGGCAGAGAAAGTCTGGCTGGCTCAG
AAGGATGGATTTACTCTTGCTACGGTGCTAAAGCCAGATGAGGGAACAGCAGACCTGCCA
GCAGGAAGGGTGAGACTTTGCATTGATGCTGACAAAACCATCACTGAGGTGGATGAGGAG
CATGTCCATCGGGCCAACCCTCCTGAGCTGGACCAGGTCGAGGACCTGGCCTCTCTCATC
AGTGTCAACGAATCCAGTGTCCTGAACACGCTTCTGCAGCGCTACAAAGCTCAGCTGCTG
CACACCTGCACAGGGCCTGATCTGATTGTCCTCCAGCCCCGGGGGCCCTCGGTGCCTTCT
GCAGGGAAGGTGCCCAAGGGCCGCCGGGATGGCCTGCCTGCCCACATTGGCTCCATGGCA
CAGCGGGCATACTGGGCGCTGCTGAACCAGCGGAGAGACCAGAGCATTGTGGCCCTGGGC
CGGAGTGGCGCTGGGAAGACCACCTGCTGTGAGCAGGTCCTGGAACACCTGGTGGGGATG
GCAGGCAGTGTGGATGGCAGGGTCTCAGTGGAGAAGATCCGAGCCACCTTCACTGTCCTC
CGGGCCTTCGGCTCTGTGTCCATGGCCCACAGCCGCAGTGCCACCCGGTTCTCCATGGTG
ATGTCGCTGGACTTCAACGCTACAGGCCGCATCACAGCTGCTCAGCTCCAGACAATGCTT
TTGGAGAAGAGCCGCGTGGCACGGCAGCCGGAAGGGGAAAGTAACTTCCTGGTTTTCTCC
CAGATGCTGGCTGGATTGGACTTGGATCTCAGGACGGAGCTGAACCTGCACCAGATGGCA
GATAGCAGCTCCTTTGGCATGGGCGTGTGGTCCAAGCCTGAAGATAAACAGAAGGCGGCA
GCTGCCTTTGCCCAGCTCCAGGGTGCCATGGAGATGCTGGGCATCTCAGAGAGCGAGCAG
CGGGCTGTTTGGCGGGTCCTGACAGCCATCTACCACCTGGGCGCGGCGGGGGCCTGCAAA
GTGGGTCGGAAGCAGTTCATGAGGTTTGAGTGGGCAAACTACGCAGCTGAGGCCCTGGGC
TGCGAGTATGAGGAGCTGAACACGGCCACCTTCAAGCACCACCTTCGACAGATCATCCAG
CAAATGACGTTTGGGCCAAGCCGATGGGGCCTCGAGGATGAGGAAACCAGCTCAGGGCTC
AAGATGACAGGAGTGGACTGTGTGGAGGGGATGGCCTCGGGCCTGTACCAGGAACTCTTT
GCGGCTGTGGTCTCACTCATCAACAGATCCTTTTCCTCCCACCATCTCTCCATGGCCTCC
ATCATGGTGGTGGACTCTCCAGGCTTCCAGAACCCCCGGCACCAGGGCAAGGACCGGGCG
GCCACCTTTGAGGAGCTGTGCCACAACTACGCCCATGAGCGCCTGCAGCTGCTGTTCTAC
CAGCGGACCTTTGTCTCCACGCTACAGCGATATCAAGAGGAAGGTGTTCCTGTGCAGTTT
GACCTCCCGGACCCCTCCCCAGGGACCACCGTGGCTGTTGTGGATCAAAATCCCTCTCAG
GTCCGCTTACCAGCTGGAGGAGGTGCCCAGGATGCCAGAGGCCTTTTCTGGGTCTTAGAT
GAGGAAGTCCATGTAGAGGGCTCCAGTGACAGTGTGGTGCTCGAGCGTCTGTGTGCTGCT
TTCGAGAAGAAAGGAGCTGGGACTGAAGGGTCCTCTGCCCTGCGGACCTGTGAGCAGCCC
CTCCAGTGTGAGATTTTCCACCAGTTGGGATGGGACCCTGTGCGGTACGACCTCACGGGC
TGGCTCCACAGAGCCAAGCCCAACCTCTCGGCCCTGGATGCACCCCAGGTCCTGCAACAG
TCAAAAAGAGAGGAGCTGCGGAGTCTATTCCAGGCCCGGGCCAAGCTGCCTCCTGTGTGC
CGGGCTGTGGCAGGCCTGGAGGGCACCTCCCAGCAGGCCCTGCAGAGGAGCCGCATGGTG
AGGAGGACCTTTGCCAGCAGCCTTGCCGCGGTGAGGAGGAAAGCCCCGTGCTCCCAGATC
AAGCTGCAGATGGATGCGCTGACCAGCATGATCAAAAGGTCCCGGCTGCACTTTATCCAC
TGCCTGGTACCAAACCCTGTGGTGGAAAGCAGGAGTGGGCAGGAATCTCCACCACCACCG
CAGCCTGGTAGAGACAAGCCTGGGGCAGGTGGACCTCTGGCCCTGGATATCCCAGCACTG
AGGGTCCAGCTTGCTGGGTTCCACATCCTGGAGGCTCTGCGTCTGCATAGGACAGGCTAT
GCTGACCACATGGGACTCACTCGCTTCCGCCGGCAATTCCAGGTGCTGGACGCTCCACTC
CTGAAGAAGCTCATGTCGACCTCCGAGGGAATAGATGAAAGGAAGGCCGTGGAGGAGCTC
CTGGAGACCCTGGATCTGGAAAAGAAGGCGGTGGCTGTGGGGCACAGCCAAGTTTTTCTC
AAGGCAGGTGTGATCTCCAGGCTTGAGAAGCAGCGAGAGAAGCTGGTATCTCAGAGCATC
GTTCTCTTCCAGGCGGCTTGCAAGGGCTTTCTGTCTCGCCAGGAATTCAAGAAGCTGAAG
ATTCGCCGACTGGCTGCACAGTGCATCCAGAAGAATGTGGCTGTGTTCCTCGCAGTCAAG
GACTGGCCATGGTGGCAGCTGCTTGGTTCCCTCCAGCCTCTACTTAGTGCCACCATTGGA
ACTGAGCAGCTCCGAGCCAAGGAGGAGGAGCTTACAACGCTAAGACGGAAGCTAGAAAAA
TCAGAGAAGTTGCGGAATGAACTCCGGCAGAACACAGATCTGCTAGAAAGCAAGATTGCT
GACTTGACCTCTGACCTTGCCGATGAGCGCTTCAAAGGTGATGTGGCCTGCCAGGTGCTG
GAGAGTGAGCGGGCAGAGCGGCTACAGGCCTTCCGGGAGGTCCAGGAGCTCAAGAGCAAG
CATGAACAAGTCCAGAAAAAACTGGGAGATGTGAATAAACAGTTGGAAGAAGCCCAGCAG
AAAATTCAGTTGAATGACTTGGAAAGGAATCCCACTGGAGGAGACGAGTGGCAGATGCGC
TTCGACTGTGCTCAGATGGAGAACGAGTTCCTCAGAAAGCGTCTGCAGCAATGCGAGGAG
AGGCTGGACTCGGAGCTGACAGCCAGGAAAGAGCTGGAGCAAAAGCTTGGGGAGTTGCAA
AGTGCTTATGACGGGGCCAAGAAGATGGCTCACCAACTGAAGAGGAAGTGCCACCATCTT
ACCTGTGACCTTGAGGATACCTGCGTCCTGCTAGAGAACCAACAAAGTCGAAACCATGAG
CTGGAGAAGAAGCAGAAGAAGTTTGACCTGCAGCTGGCCCAGGCCCTAGGTGAGTCAGTG
TTTGAGAAGGGTCTCCGTGAGAAAGTGACCCAGGAGAACACCAGTGTCCGGTGGGAGCTA
GGCCAGCTTCAGCAGCAGCTGAAGCAAAAGGAGCAGGAAGCCTCACAGCTGAAGCAGCAG
GTGGAGATGCTACAGGACCATAAACGGGAGCTGCTGGGGTCACCCTCTCTGGGGGAAAAT
TGCGTTGCTGGCTTGAAGGAGAGGCTCTGGAAGTTGGAATCCAGCGCCCTTGAGCAACAG
AAAATCCAGAGCCAGCAGGAAAACACCATCAAGCAGCTGGAGCAGCTCCGCCAGCGGTTT
GAGCTGGAGATCGAGCGGATGAAGCAGATGCACCAGAAGGACCGTGAGGACCAGGAGGAG
GAACTGGAGGATGTCCGTCAGTCCTGCCAGAAGCGGCTTCATCAGCTGGAAATGCAGCTG
GAGCAAGAGTATGAAGAGAAGCAGATGGTCCTCCATGAGAAGCAAGATTTGGAAGGCTTG
ATCGGAACCCTCTGTGACCAGATTGGCCATCGGGACTTTGATGTGGAGAAGCGACTTCGG
AGAGACCTCAGGAGGACACATGCACTGTTGTCAGACGTGCAGCTCCTTCTGGGCACCATG
GAGGATGGCAAGACATCAGTCAGCAAGGAGGAGCTGGAGAAAGTGCACAGCCAGCTGGAG
CAGAGTGAAGCCAAGTGTGAGGAGGCCTTGAAGACGCAGAAGGTGCTCACAGCGGACCTG
GAGAGTATGCACAGCGAGCTGGAGAACATGACGCGGAACAAGAGCCTGGTGGATGAGCAG
CTGTACAGGCTGCAGTTTGAGAAGGCGGACCTCCTGAAGCGCATCGATGAGGACCAGGAT
GACCTGAATGAGCTGATGCAGAAGCACAAGGACCTCATTGCTCAGTCTGCTGCTGACATT
GGGCAGATCCAAGAACTGCAGCTGCAGCTGGAGGAAGCCAAGAAGGAGAAGCACAAGCTA
CAAGAACAACTGCAGGTGGCTCAGATGCGCATCGAGTACCTGGAACAGTCCACCGTGGAT
CGAGCCATCGTCAGCAGGCAGGAGGCGGTCATCTGTGACCTAGAGAACAAGACAGAGTTC
CAGAAGGTGCAGATTAAGAGATTTGAGGTCCTGGTGATCCGGCTTCGGGACAGCCTGATC
AAGATGGGGGAGGAGCTTTCACAGGCGGCCACCTCCGAGTCCCAGCAGCGGGAGAGCAGC
CAGTACTACCAGCGGCGCCTGGAAGAGCTGAAGGCCGACATGGAAGAGCTGGTGCAGCGG
GAGGCAGAGGCCAGCCGGCGGTGCATGGAGCTGGAGAAGTACGTGGAGGAACTTGCAGCA
GTGAGGCAAACCCTCCAGACAGACCTGGAGACATCCATTCGGCGGATTGCCGACCTGCAG
GCTGCCTTGGAAGAAGTGGCATCCAGTGACAGTGATACTGAGAGTGTCCAGACGGCAGTG
GATTGTGGCAGCAGCGGCCGAAAAGAGATGGATAACGTCTCCATCCTCAGCTCCCAGCCA
GAGGGCAGCCTGCAGTCCTGGTTGAGCTGTACTCTGTCCCTGGCCACAGATACTATGAGG
ACTCCTTCTCGACAGTCAGCCACCAGCAGCCGCATCCTCAGCCCCAGGATAAACGAAGAG
GCTGGGGACACTGAGAGGACCCAGTCGGCATTGGCACTGAGCAGAGCCCGGTCCACCAAT
GTCCACAGCAAGACCTCAGGAGACAAGCCTGTTTCTCCCCACTTTGTCCGCCGGCAAAAG
TACTGTCATTTTGGGGACGGCGAAGTGCTTGCCGTCCAGAGAAAGTCCACAGAGAGATTA
GAACCTGCTTCCTCTCCCCTGGCTTCTCGGAGTACAAATACATCCCCGCTGTCGAGGGAA
AAGCTGCCCAGTCCTTCAGCGGCCCTCTCGGAGTTCGTGGAAGGGCTCCGGAGGAAGAGA
GCCCAGAGAGGCCAGGGGTCCACGCTGGGCCTAGAGGACTGGCCCACTCTCCCCATTTAC
CAGACGACTGGGGCCTCCACACTAAGGAGGGGCAGGGCTGGCAGTGACGAGGGAAACCTC
TCGCTGAGGGTTGGGGCAAAGTCACCCCTGGAAATCGAAGGGGCCGCTGGTGGTCTCTTG
AGGTCCACCAGCCTCAAATGCATCTCTTCAGACGGTGTTGGGGGCACAACCCTACTCCCC
GAAAAGTCGAAAACCCGATTCAGTTCCTGCGAGTCCCTCTTAGAATCCAGACCGAGCATG
GGGAGAAAACTGAGCTCTCCGACCACACCCAGGGACATGCTGTTGTCGCCCACACTGCGT
CCTCGGAGGCGGTGTCTGGAGTCCTCTGTGGACGATGCGGGCTGTCCAGACCTTGGAAAG
GAGCCGCTTGTTTTCCAGAACCGCCAGTTTGCCCACCTGATGGAGGAACCTCTAGGCAGT
GACCCATTCAGCTGGAAACTCCCAAGCCTCGACTACGAACGCAAGACCAAAGTGGACTTC
GATGACTTCCTCCCAGCTATCCGGAAGCCCCAGACACCTACCTCCTTGGCTGGATCAGCC
AAAGGTGGGCAAGACGGTTCACAGCGTTCAAGCATCCACTTTGAAACGGAAGAGGCTAAC
CGTTCCTTTCTCTCGGGGATCAAGACCATTTTGAAGAAGAGCCCGGAGCCCAAGGAGGAT
CCCGCTCACCTGTCTGACTCGTCCTCATCCTCCGGCTCCATCGTGTCCTTCAAAAGTGCT
GACAGCATCAAAAGTCGACCAGGAATCCCACGACTTGCGGGTGACGGTGGCGAGCGAACG
TCCCCCGAGCGGAGAGAGCCAGGGACGGGGAGGAAAGACGACGATGTTGCGAGCATAATG
AAGAAATACCTCCAGAAG
Protein sequence predicted based on nucleotide sequence
MAISSRLALWEQKIREEDKSPPPSSPPPLFSVIPGGFIKQLVRGTEKEAKEARQRKQLAV ASPEREIPEISISQPNSKSSSGTRSGSQQISQDDQSSSPGSSDILGKESEGSRSPDPEQM TSINGEKAQELGSSATPTKKTVPFKRGVRRGDVLLMVAKLDPDSAKPEKTHPHDAPPCKT SPPATDTGKEKKGETSRTPCGSQASTEILAPKAEKTRTGGLGDPGQGTVALKKGEEGQSI VGKGLGTPKTTELKEAEPQGKDRQGTRPQAQGPGEGVRPGKAEKEGAEPTNTVEKGNVSK DVGSEGKHVRPQIPGRKWGGFLGRRSKWDGPQNKKDKEGVLLSKAEKTGEPQTQMEKTSQ VQGELGDDLRMGEKAGELRSTTGKAGESWDKKEKMGQPQGKSGNAGEARSQTEKGCEAPK EVSTMVESPAAPGKGGWPGSRGQEAEEPCSRAGDGAGALETELEGPSQPALEKDAERPRI RKENQDGPAPQEEGKGGQSRDSDQAPEDRWYEAEKVWLAQKDGFTLATVLKPDEGTADLP AGRVRLCIDADKTITEVDEEHVHRANPPELDQVEDLASLISVNESSVLNTLLQRYKAQLL HTCTGPDLIVLQPRGPSVPSAGKVPKGRRDGLPAHIGSMAQRAYWALLNQRRDQSIVALG RSGAGKTTCCEQVLEHLVGMAGSVDGRVSVEKIRATFTVLRAFGSVSMAHSRSATRFSMV MSLDFNATGRITAAQLQTMLLEKSRVARQPEGESNFLVFSQMLAGLDLDLRTELNLHQMA DSSSFGMGVWSKPEDKQKAAAAFAQLQGAMEMLGISESEQRAVWRVLTAIYHLGAAGACK VGRKQFMRFEWANYAAEALGCEYEELNTATFKHHLRQIIQQMTFGPSRWGLEDEETSSGL KMTGVDCVEGMASGLYQELFAAVVSLINRSFSSHHLSMASIMVVDSPGFQNPRHQGKDRA ATFEELCHNYAHERLQLLFYQRTFVSTLQRYQEEGVPVQFDLPDPSPGTTVAVVDQNPSQ VRLPAGGGAQDARGLFWVLDEEVHVEGSSDSVVLERLCAAFEKKGAGTEGSSALRTCEQP LQCEIFHQLGWDPVRYDLTGWLHRAKPNLSALDAPQVLQQSKREELRSLFQARAKLPPVC RAVAGLEGTSQQALQRSRMVRRTFASSLAAVRRKAPCSQIKLQMDALTSMIKRSRLHFIH CLVPNPVVESRSGQESPPPPQPGRDKPGAGGPLALDIPALRVQLAGFHILEALRLHRTGY ADHMGLTRFRRQFQVLDAPLLKKLMSTSEGIDERKAVEELLETLDLEKKAVAVGHSQVFL KAGVISRLEKQREKLVSQSIVLFQAACKGFLSRQEFKKLKIRRLAAQCIQKNVAVFLAVK DWPWWQLLGSLQPLLSATIGTEQLRAKEEELTTLRRKLEKSEKLRNELRQNTDLLESKIA DLTSDLADERFKGDVACQVLESERAERLQAFREVQELKSKHEQVQKKLGDVNKQLEEAQQ KIQLNDLERNPTGGDEWQMRFDCAQMENEFLRKRLQQCEERLDSELTARKELEQKLGELQ SAYDGAKKMAHQLKRKCHHLTCDLEDTCVLLENQQSRNHELEKKQKKFDLQLAQALGESV FEKGLREKVTQENTSVRWELGQLQQQLKQKEQEASQLKQQVEMLQDHKRELLGSPSLGEN CVAGLKERLWKLESSALEQQKIQSQQENTIKQLEQLRQRFELEIERMKQMHQKDREDQEE ELEDVRQSCQKRLHQLEMQLEQEYEEKQMVLHEKQDLEGLIGTLCDQIGHRDFDVEKRLR RDLRRTHALLSDVQLLLGTMEDGKTSVSKEELEKVHSQLEQSEAKCEEALKTQKVLTADL ESMHSELENMTRNKSLVDEQLYRLQFEKADLLKRIDEDQDDLNELMQKHKDLIAQSAADI GQIQELQLQLEEAKKEKHKLQEQLQVAQMRIEYLEQSTVDRAIVSRQEAVICDLENKTEF QKVQIKRFEVLVIRLRDSLIKMGEELSQAATSESQQRESSQYYQRRLEELKADMEELVQR EAEASRRCMELEKYVEELAAVRQTLQTDLETSIRRIADLQAALEEVASSDSDTESVQTAV DCGSSGRKEMDNVSILSSQPEGSLQSWLSCTLSLATDTMRTPSRQSATSSRILSPRINEE AGDTERTQSALALSRARSTNVHSKTSGDKPVSPHFVRRQKYCHFGDGEVLAVQRKSTERL EPASSPLASRSTNTSPLSREKLPSPSAALSEFVEGLRRKRAQRGQGSTLGLEDWPTLPIY QTTGASTLRRGRAGSDEGNLSLRVGAKSPLEIEGAAGGLLRSTSLKCISSDGVGGTTLLP EKSKTRFSSCESLLESRPSMGRKLSSPTTPRDMLLSPTLRPRRRCLESSVDDAGCPDLGK EPLVFQNRQFAHLMEEPLGSDPFSWKLPSLDYERKTKVDFDDFLPAIRKPQTPTSLAGSA KGGQDGSQRSSIHFETEEANRSFLSGIKTILKKSPEPKEDPAHLSDSSSSSGSIVSFKSA DSIKSRPGIPRLAGDGGERTSPERREPGTGRKDDDVASIMKKYLQK
Coding Sequence Details
Insert Sequence Verified?:
N
Verification Method:
Verified by the ORFeome collaboration
Mutations:
None
Discrepancy:
None
5' Linker Sequence:
None
3' Linker Sequence:
None
Recommended Growth Condition:
Distributed in bacterial strain:
DH5-alpha T1 phage resistant
Antibiotic Selection:
Host Type:
bacterial
Marker:
spectinomycin
Bacterial Selection Condition:
100 ug/mL spectinomycin
Growth Condition:
Growth at 37 degrees C in LB supplemented with 100 ug/mL spectinomycin.
Comments:
None
Protein Expression Results:
None
Recommended expression in:
Not Applicable
Vector Name:
pENTR223
Synonyms:
pDONR223, pENTR223-XnCDSYn
Description:
Gateway type (lambda att-type) recombinational cloning entry (master) vector; spectinomycin resistance; recombinational cloning.
Comments:
Additional information about this vector is available from the commercial website and at the ORFeome website http://orfeomecollaboration.org/vectors.php; also see supplemental figure 1 of the paper PubMed ID 15489335.
Size (bp):
2815
Parent Vector:
None
Properties:
Gateway, donor (entry), recombinational cloning
Author Name:
Mark Vidal
Publications:
PMID:
15489335
Title: Human ORFeome Version 1.1: A Platform for Reverse Proteomics
Vector Map:
Vector Sequence:
Sequence:
Vector Features:
Type
Name
Description
Start
End
bacterial origin
ori
bacterial origin of replication
2220
2753
gene fragment
pas
5p stem of pBR primose assembly site
63
73
gene fragment
pas prime
3p stem of pBR pas(L)
105
116
recombination site
attL1
attL1 recombination site
570
665
recombination site
attL2
attL2 recombination site
793
696
selectable marker
SpnR
spectinomycin resistance gene
1062
2072
trxn termination sequence
T1-T2
rrnBT1 and T2 terminators
510
224
