DICER1 (Homo sapiens) in pENTR223 (Gateway donor/master vector)

Explanation of Terms

Gene:
Gene Symbol:  DICER1
Gene Name:  dicer 1, ribonuclease type III
Original Clone ID:
ccsbBroadEn_02759
Keyword:
None
Species:
Homo sapiens
Type:
cDNA
Vector Name:
pENTR223
Format: 
FUSION
Source:
The ORFeome Collaboration
Description:
Original GenBank Accession: BC150287.1
Comments:
None
Mutation / Discrepancy:
No / No
Publications:
None
Authors:
Harvard Medical School
Dana-Farber Cancer Institute
Broad Institute of Harvard and Massachusetts Institute of Technology (MIT)
David E Hill
David E Root
The ORFeome Collaboration
HsCD00516508
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AVAILABLE
No restriction
Special MTA: None
Insert sequence: 5767nts
Open reading frame: 1 to 5767

ATGAAAAGCCCTGCTTTGCAACCCCTCAGCATGGCAGGCCTGCAGCTCATGACCCCTGCT
TCCTCACCAATGGGTCCTTTCTTTGGACTGCCATGGCAACAAGAAGCAATTCATGATAAC
ATTTATACGCCAAGAAAATATCAGGTTGAACTGCTTGAAGCAGCTCTGGATCATAATACC
ATCGTCTGTTTAAACACTGGCTCAGGGAAGACATTTATTGCAGTACTACTCACTAAAGAG
CTGTCCTATCAGATCAGGGGAGACTTCAGCAGAAATGGAAAAAGGACGGTGTTCTTGGTC
AACTCTGCAAACCAGGTTGCTCAACAAGTGTCAGCTGTCAGAACTCATTCAGATCTCAAG
GTTGGGGAATACTCAAACCTAGAAGTAAATGCATCTTGGACAAAAGAGAGATGGAACCAA
GAGTTTACTAAGCACCAGGTTCTCATTATGACTTGCTATGTCGCCTTGAATGTTTTGAAA
AATGGTTACTTATCACTGTCAGACATTAACCTTTTGGTGTTTGATGAGTGTCATCTTGCA
ATCCTAGACCACCCCTATCGAGAAATTATGAAGCTCTGTGAAAATTGTCCATCATGTCCT
CGCATTTTGGGACTAACTGCTTCCATTTTAAATGGGAAATGTGATCCAGAGGAATTGGAA
GAAAAGATTCAGAAACTAGAGAAAATTCTTAAGAGTAATGCTGAAACTGCAACTGACCTG
GTGGTCTTAGACAGGTATACTTCTCAGCCATGTGAGATTGTGGTGGATTGTGGACCATTT
ACTGACAGAAGTGGGCTTTATGAAAGACTGCTGATGGAATTAGAAGAAGCACTTAATTTT
ATCAATGATTGTAATATATCTGTACATTCAAAAGAAAGAGATTCTACTTTAATTTCGAAA
CAGATACTATCAGACTGTCGTGCCGTATTGGTAGTTCTGGGACCCTGGTGTGCAGATAAA
GTAGCTGGAATGATGGTAAGAGAACTACAGAAATACATCAAACATGAGCAAGAGGAGCTG
CACAGGAAATTTTTATTGTTTACAGACACTTTCCTAAGGAAAATACATGCACTATGTGAA
GAGCACTTCTCACCTGCCTCACTTGACCTGAAATTTGTAACTCCTAAAGTAATCAAACTG
CTCGAAATCTTACGCAAATATAAACCATATGAGCGACAGCAGTTTGAAAGCGTTGAGTGG
TATAATAATAGAAATCAGGATAATTATGTGTCATGGAGTGATTCTGAGGATGATGATGAG
GATGAAGAAATTGAAGAAAAAGAGAAGCCAGAGACAAATTTTCCTTCTCCTTTTACCAAC
ATTTTGTGCGGAATTATTTTTGTGGAAAGAAGATACACAGCAGTTGTCTTAAACAGATTG
ATAAAGGAAGCTGGCAAACAAGATCCAGAGCTGGCTTATATCAGTAGCAATTTCATAACT
GGACATGGCATTGGGAAGAATCAGCCTCGCAACAAACAGATGGAAGCAGAATTCAGAAAA
CAGGAAGAGGTACTTAGGAAATTTCGAGCACATGAGACCAACCTGCTTATTGCAACAAGT
ATTGTAGAAGAGGGTGTTGATATACCAAAATGCAACTTGGTGGTTCGTTTTGATTTGCCC
ACAGAATATCGATCCTATGTTCAATCTAAAGGAAGAGCAAGGGCACCCATCTCTAATTAT
ATAATGTTAGCGGATACAGACAAAATAAAAAGTTTTGAAGAAGACCTTAAAACCTACAAA
GCTATTGAAAAGATCTTGAGAAACAAGTGTTCCAAGTCGGTTGATACTGGTGAGACTGAC
ATTGATCCTGTCATGGATGATGATGACGTTTTCCCACCATATGTGTTGAGGCCTGACGAT
GGTGGTCCACGAGTCACAATCAACACGGCCATTGGACACATCAATAGATACTGTGCTAGA
TTACCAAGTGATCCGTTTACTCATCTAGCTCCTAAATGCAGAACCCGAGAGTTGCCTGAT
GGTACATTTTATTCAACTCTTTATCTGCCAATTAACTCACCTCTTCGAGCCTCCATTGTT
GGTCCACCAATGAGCTGTGTACGATTGGCTGAAAGAGTTGTAGCTCTCATTTGCTGTGAG
AAACTGCACAAAATTGGCGAACTGGATGACCATTTGATGCCAGTTGGGAAAGAGACTGTT
AAATATGAAGAGGAGCTTGATTTGCATGATGAAGAAGAGACCAGTGTTCCAGGAAGACCA
GGTTCCACGAAACGAAGGCAGTGCTACCCAAAAGCAATTCCAGAGTGTTTGAGGGATAGT
TATCCCAGACCTGATCAGCCCTGTTACCTGTATGTGATAGGAATGGTTTTAACTACACCT
TTACCTGATGAACTCAACTTTAGAAGGCGGAAGCTCTATCCTCCTGAAGATACCACAAGA
TGCTTTGGAATACTGACGGCCAAACCCATACCTCAGATTCCACACTTTCCTGTGTACACA
CGCTCTGGAGAGGTTACCATATCCATTGAGTTGAAGAAGTCTGGTTTCATGTTGTCTCTA
CAAATGCTTGAGTTGATTACAAGACTTCACCAGTATATATTCTCACATATTCTTCGGCTT
GAAAAACCTGCACTAGAATTTAAACCTACAGACGCTGATTCAGCATACTGTGTTCTACCT
CTTAATGTTGTTAATGACTCCAGCACTTTGGATATTGACTTTAAATTCATGGAAGATATT
GAGAAGTCTGAAGCTCGCATAGGCATTCCCAGTACAAAGTATACAAAAGAAACACCCTTT
GTTTTTAAATTAGAAGATTACCAAGATGCCGTTATCATTCCAAGATATCGCAATTTTGAT
CAGCCTCATCGATTTTATGTAGCTGATGTGTACACTGATCTTACCCCACTCAGTAAATTT
CCTTCCCCTGAGTATGAAACTTTTGCAGAATATTATAAAACAAAGTACAACCTTGACCTA
ACCAATCTCAACCAGCCACTGCTGGATGTGGACCACACATCTTCAAGACTTAATCTTTTG
ACACCTCGACATTTGAATCAGAAGGGGAAAGCGCTTCCTTTAAGCAGTGCTGAGAAGAGG
AAAGCCAAATGGGAAAGTCTGCAGAATAAACAGATACTGGTTCCAGAACTCTGTGCTATA
CATCCAATTCCAGCATCACTGTGGAGAAAAGCTGTTTGTCTCCCCAGCATACTTTATCGC
CTTCACTGCCTTTTGACTGCAGAGGAGCTAAGAGCCCAGACTGCCAGCGATGCTGGCGTG
GGAGTCAGATCACTTCCTGCGGATTTTAGATACCCTAACTTAGACTTCGGGTGGAAAAAA
TCTATTGACAGCAAATCTTTCATCTCAATTTCTAACTCCTCTTCAGCTGAAAATGATAAT
TACTGTAAGCACAGCACAATTGTCCCTGAAAATGCTGCACATCAAGGTGCTAATAGAACC
TCCTCTCTAGAAAATCATGACCAAATGTCTGTGAACTGCAGAACGTTGCTCAGCGAGTCC
CCTGGTAAGCTCCACGTTGAAGTTTCAGCAGATCTTACAGCAATTAATGGTCTTTCTTAC
AATCAAAATCTCGCCAATGGCAGTTATGATTTAGCTAACAGAGACTTTTGCCAAGGAAAT
CAGCTAAATTACTACAAGCAGGAAATACCCGTGCAACCAACTACCTCATATTCCATTCAG
AATTTATACAGTTACGAGAACCAGCCCCAGCCCAGCGATGAATGTACTCTCCTGAGTAAT
AAATACCTTGATGGAAATGCTAACAAATCTACCTCAGATGGAAGTCCTGTGATGGCCGTA
ATGCCTGGTACGACAGACACTATTCAAGTGCTCAAGGGCAGGATGGATTCTGAGCAGAGC
CCTTCTATTGGGTACTCCTCAAGGACTCTTGGCCCCAATCCTGGACTTATTCTTCAGGCT
TTGACTCTGTCAAACGCTAGTGATGGATTTAACCTGGAGCGGCTTGAAATGCTTGGCGAC
TCCTTTTTAAAGCATGCCATCACCACATATCTATTTTGCACTTACCCTGATGCGCATGAG
GGCCGCCTTTCATATATGAGAAGCAAAAAGGTCAGCAACTGTAATCTGTATCGCCTTGGA
AAAAAGAAGGGACTACCCAGCCGCATGGTGGTGTCAATATTTGATCCCCCTGTGAATTGG
CTTCCTCCTGGTTATGTAGTAAATCAAGACAAAAGCAACACAGATAAATGGGAAAAAGAT
GAAATGACAAAAGACTGCATGCTGGCGAATGGCAAACTGGATGAGGATTACGAGGAGGAG
GATGAGGAGGAGGAGAGCCTGATGTGGAGGGCTCCGAAGGAAGAGGCTGACTATGAAGAT
GATTTCCTGGAGTATGATCAGGAACATATCAGATTTATAGATAATATGTTAATGGGGTCA
GGAGCTTTTGTAAAGAAAATCTCTCTTTCTCCTTTTTCAACCACTGATTCTGCATATGAA
TGGAAAATGCCCAAAAAATCCTCCTTAGGTAGTATGCCATTTTCATCAGATTTTGAGGAT
TTTGACTACAGCTCTTGGGATGCAATGTGCTATCTGGATCCTAGCAAAGCTGTTGAAGAA
GATGACTTTGTGGTGGGGTTCTGGAATCCATCAGAAGAAAACTGTGGTGTTGACACGGGA
AAGCAGTCCATTTCTTACGACTTGCACACTGAGCAGTGTATTGCTGACAAAAGCATAGCG
GACTGTGTGGAAGCCCTGCTGGGCTGCTATTTAACCAGCTGTGGGGAGAGGGCTGCTCAG
CTTTTCCTCTGTTCACTGGGGCTGAAGGTGCTCCCGGTAATTAAAAGGACTGATCGGGAA
AAGGCCCTGTGCCCTACTCGGGAGAATTTCAACAGCCAACAAAAGAACCTTTCAGTGAGC
TGTGCTGCTGCTTCTGTGGCCAGTTCACGCTCTTCTGTATTGAAAGACTCGGAATATGGT
TGTTTGAAGATTCCACCAAGATGTATGTTTGATCATCCAGATGCAGATAAAACACTGAAT
CACCTTATATCGGGGTTTGAAAATTTTGAAAAGAAAATCAACTACAGATTCAAGAATAAG
GCTTACCTTCTCCAGGCTTTTACACATGCCTCCTACCACTACAATACTATCACTGATTGT
TACCAGCGCTTAGAATTCCTGGGAGATGCGATTTTGGACTACCTCATAACCAAGCACCTT
TATGAAGACCCGCGGCAGCACTCCCCGGGGGTCCTGACAGACCTGCGGTCTGCCCTGGTC
AACAACACCATCTTTGCATCGCTGGCTGTAAAGTACGACTACCACAAGTACTTCAAAGCT
GTCTCTCCTGAGCTCTTCCATGTCATTGATGACTTTGTGCAGTTTCAGCTTGAGAAGAAT
GAAATGCAAGGAATGGATTCTGAGCTTAGGAGATCTGAGGAGGATGAAGAGAAAGAAGAG
GATATTGAAGTTCCAAAGGCCATGGGGGATATTTTTGAGTCGCTTGCTGGTGCCATTTAC
ATGGATAGTGGGATGTCACTGGAGACAGTCTGGCAGGTGTACTATCCCATGATGCGGCCA
CTAATAGAAAAGTTTTCTGCAAATGTACCCCGTTCCCCTGTGCGAGAATTGCTTGAAATG
GAACCAGAAACTGCCAAATTTAGCCCGGCTGAGAGAACTTACGACGGGAAGGTCAGAGTC
ACTGTGGAAGTAGTAGGAAAGGGGAAATTTAAAGGTGTTGGTCGAAGTTACAGGATTGCC
AAATCTGCAGCAGCAAGAAGAGCCCTCCGAAGCCTCAAAGCTAATCAACCTCAGGTTCCC
AATAGC

Protein sequence predicted based on nucleotide sequence

MKSPALQPLSMAGLQLMTPASSPMGPFFGLPWQQEAIHDNIYTPRKYQVELLEAALDHNT
IVCLNTGSGKTFIAVLLTKELSYQIRGDFSRNGKRTVFLVNSANQVAQQVSAVRTHSDLK
VGEYSNLEVNASWTKERWNQEFTKHQVLIMTCYVALNVLKNGYLSLSDINLLVFDECHLA
ILDHPYREIMKLCENCPSCPRILGLTASILNGKCDPEELEEKIQKLEKILKSNAETATDL
VVLDRYTSQPCEIVVDCGPFTDRSGLYERLLMELEEALNFINDCNISVHSKERDSTLISK
QILSDCRAVLVVLGPWCADKVAGMMVRELQKYIKHEQEELHRKFLLFTDTFLRKIHALCE
EHFSPASLDLKFVTPKVIKLLEILRKYKPYERQQFESVEWYNNRNQDNYVSWSDSEDDDE
DEEIEEKEKPETNFPSPFTNILCGIIFVERRYTAVVLNRLIKEAGKQDPELAYISSNFIT
GHGIGKNQPRNKQMEAEFRKQEEVLRKFRAHETNLLIATSIVEEGVDIPKCNLVVRFDLP
TEYRSYVQSKGRARAPISNYIMLADTDKIKSFEEDLKTYKAIEKILRNKCSKSVDTGETD
IDPVMDDDDVFPPYVLRPDDGGPRVTINTAIGHINRYCARLPSDPFTHLAPKCRTRELPD
GTFYSTLYLPINSPLRASIVGPPMSCVRLAERVVALICCEKLHKIGELDDHLMPVGKETV
KYEEELDLHDEEETSVPGRPGSTKRRQCYPKAIPECLRDSYPRPDQPCYLYVIGMVLTTP
LPDELNFRRRKLYPPEDTTRCFGILTAKPIPQIPHFPVYTRSGEVTISIELKKSGFMLSL
QMLELITRLHQYIFSHILRLEKPALEFKPTDADSAYCVLPLNVVNDSSTLDIDFKFMEDI
EKSEARIGIPSTKYTKETPFVFKLEDYQDAVIIPRYRNFDQPHRFYVADVYTDLTPLSKF
PSPEYETFAEYYKTKYNLDLTNLNQPLLDVDHTSSRLNLLTPRHLNQKGKALPLSSAEKR
KAKWESLQNKQILVPELCAIHPIPASLWRKAVCLPSILYRLHCLLTAEELRAQTASDAGV
GVRSLPADFRYPNLDFGWKKSIDSKSFISISNSSSAENDNYCKHSTIVPENAAHQGANRT
SSLENHDQMSVNCRTLLSESPGKLHVEVSADLTAINGLSYNQNLANGSYDLANRDFCQGN
QLNYYKQEIPVQPTTSYSIQNLYSYENQPQPSDECTLLSNKYLDGNANKSTSDGSPVMAV
MPGTTDTIQVLKGRMDSEQSPSIGYSSRTLGPNPGLILQALTLSNASDGFNLERLEMLGD
SFLKHAITTYLFCTYPDAHEGRLSYMRSKKVSNCNLYRLGKKKGLPSRMVVSIFDPPVNW
LPPGYVVNQDKSNTDKWEKDEMTKDCMLANGKLDEDYEEEDEEEESLMWRAPKEEADYED
DFLEYDQEHIRFIDNMLMGSGAFVKKISLSPFSTTDSAYEWKMPKKSSLGSMPFSSDFED
FDYSSWDAMCYLDPSKAVEEDDFVVGFWNPSEENCGVDTGKQSISYDLHTEQCIADKSIA
DCVEALLGCYLTSCGERAAQLFLCSLGLKVLPVIKRTDREKALCPTRENFNSQQKNLSVS
CAAASVASSRSSVLKDSEYGCLKIPPRCMFDHPDADKTLNHLISGFENFEKKINYRFKNK
AYLLQAFTHASYHYNTITDCYQRLEFLGDAILDYLITKHLYEDPRQHSPGVLTDLRSALV
NNTIFASLAVKYDYHKYFKAVSPELFHVIDDFVQFQLEKNEMQGMDSELRRSEEDEEKEE
DIEVPKAMGDIFESLAGAIYMDSGMSLETVWQVYYPMMRPLIEKFSANVPRSPVRELLEM
EPETAKFSPAERTYDGKVRVTVEVVGKGKFKGVGRSYRIAKSAAARRALRSLKANQPQVP
NS

Coding Sequence Details

Insert Sequence Verified?:
N
Verification Method:
Verified by the ORFeome collaboration
Mutations:
None
Discrepancy:
None
5' Linker Sequence:
None
3' Linker Sequence:
None

Recommended Growth Condition:

Distributed in bacterial strain:
DH5-alpha T1 phage resistant
Antibiotic Selection:
Host Type:  bacterial    Marker:  spectinomycin
Bacterial Selection Condition: 100 ug/mL spectinomycin
Growth Condition: Growth at 37 degrees C in LB supplemented with 100 ug/mL spectinomycin.
Comments: None
Protein Expression Results:
None
Recommended expression in:
Not Applicable