NCgl0548 (C. glutamicum ATCC 13032 )
In pSGX3 (His-tagged bacterial expression vector)

Explanation of Terms

Gene:
Gene Symbol:  NCgl0548
Gene Name:  cyclopropane-fatty-acyl-phospholipid synthase
Original Clone ID:
11120j4BCt9p1
Keyword:
partial length cds 123-434 of 435 aa
Species:
Corynebacterium glutamicum ATCC 13032
Type:
cDNA
Vector Name:
pSGX3
Format: 
CLOSED
Source:
New York SGX Research Center for Structural Genomics
Description:
None
Comments:
Linker not verified.
Mutation / Discrepancy:
No / No
Publications:
None
Authors:
PSI
New York SGX Research Center for Structural Genomics
CgCD00519314
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AVAILABLE
No restriction
Special MTA: PSI
Insert sequence: 963nts
Open reading frame: 1 to 963

GAAATTCCACCAATGGAACAAGCCCCTGCATGGCGTAAAGCACTGATCAACGGGCTAGCA
TCCAGGCACTCGAAATCCCGCGACAAGAAAGCCATTAGCTACCACTACGACGTGGGCAAT
GAGTTCTACTCCCTGTTTTTAGATGATTCCATGACCTATACCTGCGCGTATTATCCAACG
CCAGAATCAAGTTTGGAAGAAGCCCAAGAAAACAAATACCGCCTCATCTTTGAAAAACTG
CGTCTGAAAGAAGGCGATCGCCTCCTAGACGTGGGATGCGGTTGGGGAGGCATGGTCCGC
TACGCCGCCAAACACGGTGTGAAAGCCATCGGAGTTACGCTGTCTGAACAGCAATATGAG
TGGGGTCAAGCAGAGATCAAACGCCAAGGTTTGGAAGACCTCGCGGAAATTCGCTTCATG
GATTACCGCGATGTTCCAGAAACTGGATTCGATGCGATCTCAGCAATCGGCATCATTGAA
CACATCGGTGTGAACAACTATCCCGACTACTTTGAATTGCTCAGCAGCAAACTCAAAACA
GGCGGACTGATGCTCAACCACAGCATCACCTACCCAGACAACCGCCCCCGCCACGCAGGT
GCATTTATTGATCGCTACATTTTCCCCGACGGTGAACTCACTGGCTCTGGCACCCTGATC
AAGCACATGCAGGACAACGGTTTCGAAGTGCTGCACGAAGAAAACCTCCGCTTTGATTAC
CAACGCACCCTGCACGCGTGGTGCGAAAACCTCAAAGAAAATTGGGAGGAAGCAGTTGAA
CTCGCCGGTGAACCCACTGCACGACTCTTTGGCCTGTACATGGCAGGTTCGGAATGGGGA
TTTGCCCACAACATCGTCCAGCTGCACCAAGTACTGGGTGTGAAACTCGATGAGCAGGGA
AGTCGCGGAGAAGTTCCTGAAAGAATGTGGTGGACTGAAGGGCATCATCACCATCACCAC
TGA

Protein sequence predicted based on nucleotide sequence

EIPPMEQAPAWRKALINGLASRHSKSRDKKAISYHYDVGNEFYSLFLDDSMTYTCAYYPT
PESSLEEAQENKYRLIFEKLRLKEGDRLLDVGCGWGGMVRYAAKHGVKAIGVTLSEQQYE
WGQAEIKRQGLEDLAEIRFMDYRDVPETGFDAISAIGIIEHIGVNNYPDYFELLSSKLKT
GGLMLNHSITYPDNRPRHAGAFIDRYIFPDGELTGSGTLIKHMQDNGFEVLHEENLRFDY
QRTLHAWCENLKENWEEAVELAGEPTARLFGLYMAGSEWGFAHNIVQLHQVLGVKLDEQG
SRGEVPERMWWTEGHHHHHH*

Coding Sequence Details

Insert Sequence Verified?:
Y
Verification Method:
Sequence Verification
Mutations:
None
Discrepancy:
c11t,a12t,P4L;t230n;
del@791,1;g811t,c813g,G271W
                                                    

5' Linker Sequence:
None
3' Linker Sequence:
None

Recommended Growth Condition:

Distributed in bacterial strain:
DH5-alpha T1 phage resistant
Antibiotic Selection:
Host Type:  bacterial    Marker:  kanamycin
Bacterial Selection Condition: 50ug/mL kanamycin
Growth Condition: Growth with the single antibiotic in LB at 37 degrees is recommended.
Comments: Conditions for Gateway-type vectors in recombined (with insert) form and other kanamycin resistant vectors.
Protein Expression Results:
ProteinExpressed: Protein_Confirmed
ProteinPurified: Protein_Purified
SolubleProtein: Protein_Soluble
Find experimental protocols in NYSGXRC-11120j
Recommended expression in:
Not Applicable