dapA (A. pernix K1 )
In pSGX3 (His-tagged bacterial expression vector)

Explanation of Terms

Gene:
Gene Symbol:  dapA
Gene Name:  dihydrodipicolinate synthase
Original Clone ID:
11102p1BCt16p1
Keyword:
full-length cds 2-292 of 303 aa
Species:
Aeropyrum pernix K1
Type:
cDNA
Vector Name:
pSGX3
Format: 
CLOSED
Source:
New York SGX Research Center for Structural Genomics
Description:
None
Comments:
Linker not verified.
Mutation / Discrepancy:
No / No
Publications:
None
Authors:
PSI
New York SGX Research Center for Structural Genomics
ApCD00519382
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AVAILABLE
No restriction
Special MTA: PSI
Insert sequence: 900nts
Open reading frame: 1 to 900

GCTAAACCTAAGGTTATAGTACCCATGGTAACCCCCCTCAGGGGCGGCTCCGTTGACAAG
AACGCCGCCTCCTGGCTCGCCTCCAGGCTGTCCGAGAGCGGTGTAGACGGGGTCTTCGTC
CCGGGCACCACGGGCGAGTGGTACCTCCTCGACCCCCACGAGAGGGCTGAGGCGTTCTTC
GCAGTAGCCTCTAGCGCCATAGCGGGGCTCAGGCTCGTGGCGGGCGTCTCGGGCTACAGG
CCTGAGGAGTCCTACAGGCTCGCCGAGGAGGCGGCGGGCCACGGGGCCCACGCGGTGATG
GCCGTGCCCCCCATCTACTTCAGGCCCTCGACAGACTTCCTGAGGGAGTTCTACGGCGAG
ATCAAGAGGAGGGCCGGAGGGCTGCCAACATACATCTACATTTTCCCCGAGAGGATGGGC
TACACCCTAGCAGTCGAGCAGGTGGAGAGCCTGGTGGAGGCGGGTGTGGTTGACGGGATC
AAGGCGACTGTGACCGACGCAGCCTACATAGCAGGCCTAGCCGCCGTTAAGGAGAGGAAC
CCCGAGTTCGAGGTCCTCGCCGGCGGCCTGGAGATGCTGGTGCACACCTCGCTCTCCGGG
GGCGACGGCGTTGTGGACGCCTACTCCAACGTAGCCCCCAAGCTGGCCGTCGAGCTGGCG
GAGAGCCTAGAGAAGGGGAGCGTTGAGAGGGTAGCGGGGCTCCAGGGGAGGGTGCTCAAG
CTCTCGCGAATACTAGGGAAGAGGAGCCTCCCCGCCGTCCTCAAGGCAGTGCTCCACGTG
CTGGGCGCCCCCATCACGGGGGAGGTCAGGAAGCCCCTCAAACCCCTGACCCAGCCAGAG
GCTAACGCCACGGCCAACGCCCTCTGCACCAGCGAAGGGCATCATCACCATCACCACTGA

Protein sequence predicted based on nucleotide sequence

AKPKVIVPMVTPLRGGSVDKNAASWLASRLSESGVDGVFVPGTTGEWYLLDPHERAEAFF
AVASSAIAGLRLVAGVSGYRPEESYRLAEEAAGHGAHAVMAVPPIYFRPSTDFLREFYGE
IKRRAGGLPTYIYIFPERMGYTLAVEQVESLVEAGVVDGIKATVTDAAYIAGLAAVKERN
PEFEVLAGGLEMLVHTSLSGGDGVVDAYSNVAPKLAVELAESLEKGSVERVAGLQGRVLK
LSRILGKRSLPAVLKAVLHVLGAPITGEVRKPLKPLTQPEANATANALCTSEGHHHHHH*

Coding Sequence Details

Insert Sequence Verified?:
Y
Verification Method:
Sequence Verification
Mutations:
None
Discrepancy:
c272t,A91V;g670c,E224Q;
ins@722,1
                                                    

5' Linker Sequence:
None
3' Linker Sequence:
None

Recommended Growth Condition:

Distributed in bacterial strain:
DH5-alpha T1 phage resistant
Antibiotic Selection:
Host Type:  bacterial    Marker:  kanamycin
Bacterial Selection Condition: 50ug/mL kanamycin
Growth Condition: Growth with the single antibiotic in LB at 37 degrees is recommended.
Comments: Conditions for Gateway-type vectors in recombined (with insert) form and other kanamycin resistant vectors.
Protein Expression Results:
ProteinExpressed: Protein_Confirmed
SolubleProtein: Protein_Soluble
ProteinPurified: Tested_Not_Purified
Find experimental protocols in NYSGXRC-11102p
Recommended expression in:
Not Applicable