MM_1154 (M. mazei Go1 )
In pSGX3 (His-tagged bacterial expression vector)

Explanation of Terms

Gene:
Gene Symbol:  MM_1154
Gene Name:  NDP-N-acetyl-D-galactosaminuronic acid dehydrogenase
Original Clone ID:
11151g3BCt4p1
Keyword:
full-length cds 18-469 of 478 aa
Species:
Methanosarcina mazei Go1
Type:
cDNA
Vector Name:
pSGX3
Format: 
CLOSED
Source:
New York SGX Research Center for Structural Genomics
Description:
None
Comments:
Linker not verified.
Mutation / Discrepancy:
No / No
Publications:
None
Authors:
PSI
New York SGX Research Center for Structural Genomics
MmCD00519398
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AVAILABLE
No restriction
Special MTA: PSI
Insert sequence: 1383nts
Open reading frame: 1 to 1383

AAAAAGATAGGCGTTCTTGGCATGGGATATGTAGGAATCCCTGCTGCTGTCCTTTTTGCA
GATGCTCCCTGTTTTGAGAAAGTCCTCGGCTTCCAGCGCAACTCAAAAAGCTCGGGTTAT
AAAATTGAAATGCTTAACAGAGGGGAGAGCCCGCTCAAAGGGGAAGAGCCCGGCCTTGAA
GAGCTTATTGGAAAGGTTGTAAAAGCCGGAAAGTTCGAATGCACACCTGATTTTTCAAGG
ATTTCCGAGCTTGATGCAGTTACACTTGCAATTCAGACTCCTTTTGCAAATCCCAAAGAC
CTGGAACCTGATTTCAGCGCGCTTATAGATGGTATCAGAAATGTGGGCAAATACCTTAAA
CCGGGGATGCTTGTGGTCCTGGAATCCACAATTACTCCCGGGACAACTGAAGGCATGGCA
AAACAGATCCTTGAAGAAGAGTCAGGGCTAAAAGCAGGTGAAGATTTTGCCCTTGCACAC
GCCCCCGAAAGAGTGATGGTAGGCAGGCTTTTAAAGAACATCAGAGAACACGACAGGATC
GTGGGCGGAATAGATGAAGCAAGCACAAAAAGGGCTGTTGAACTGTACTCCCCTGTGCTC
ACAGTCGGACAGGTTATCCCTATGAGTGCAACAGCGGCTGAGGTCACAAAAACCGCAGAA
AACACTTTTCGCGACCTTCAGATTGCAGCAATCAACCAGCTCGCCCTTTACTGTGAAGCT
ATGGGCATAAATGTCTATGATGTCAGGACAGGAGTCGACAGCTTAAAAGGCGAGGGAATT
ACAAGGGCTGTCCTCTGGCCCGGAGCAGGAGTTGGAGGCCACTGCCTGACCAAAGACACT
TACCACCTTGAAAGAGGAGTTAAAATCGGGAGGGGAGAACTTGACTATCCGGAAGGTGCA
GATTCTATTTACGTGCTTGCAAGGAAAGTCAATGATTTCATGCCCGCACACATGTATAAC
CTGACCGTTGCAGCCCTTGAAAGGCTCGGGAAGAAGATGGATGGCTCAAAAGTCGCAATG
CTCGGCTGGGCATTTATCAAAGATTCGGACGATGCCAGAAACACACCTTCGGAACCATAC
AGGGACCTCTGCCTGAAAGCCGGGGCCAGCGTAATGGTACACGACCCTTACGTTGTCAAT
TATCCGGGCGTTGAAATTTCAGATAATCTGGAAGAGGTTGTCAGAAACGCAGACGCTATA
GTTGTACTTGCAGGGCACAGTGCATACTCCAGCCTTAAAGCTGATTGGGCAAAGAAAGTA
AGCGCAAAAGCAAATCCGGTTATTATTGATGGGAGAAATGTCATCGAGCCGGACGAATTT
ATCGGGAAAGGGTTCGTTTACAAGGGAATTGGGAGGGAAGGGCATCATCACCATCACCAC
TGA

Protein sequence predicted based on nucleotide sequence

KKIGVLGMGYVGIPAAVLFADAPCFEKVLGFQRNSKSSGYKIEMLNRGESPLKGEEPGLE
ELIGKVVKAGKFECTPDFSRISELDAVTLAIQTPFANPKDLEPDFSALIDGIRNVGKYLK
PGMLVVLESTITPGTTEGMAKQILEEESGLKAGEDFALAHAPERVMVGRLLKNIREHDRI
VGGIDEASTKRAVELYSPVLTVGQVIPMSATAAEVTKTAENTFRDLQIAAINQLALYCEA
MGINVYDVRTGVDSLKGEGITRAVLWPGAGVGGHCLTKDTYHLERGVKIGRGELDYPEGA
DSIYVLARKVNDFMPAHMYNLTVAALERLGKKMDGSKVAMLGWAFIKDSDDARNTPSEPY
RDLCLKAGASVMVHDPYVVNYPGVEISDNLEEVVRNADAIVVLAGHSAYSSLKADWAKKV
SAKANPVIIDGRNVIEPDEFIGKGFVYKGIGREGHHHHHH*

Coding Sequence Details

Insert Sequence Verified?:
Y
Verification Method:
Sequence Verification
Mutations:
None
Discrepancy:
ins@7,5;del@10,4;
t15a,del@5,1;t667n;
t708n;a766n
                                                    

5' Linker Sequence:
None
3' Linker Sequence:
None

Recommended Growth Condition:

Distributed in bacterial strain:
DH5-alpha T1 phage resistant
Antibiotic Selection:
Host Type:  bacterial    Marker:  kanamycin
Bacterial Selection Condition: 50ug/mL kanamycin
Growth Condition: Growth with the single antibiotic in LB at 37 degrees is recommended.
Comments: Conditions for Gateway-type vectors in recombined (with insert) form and other kanamycin resistant vectors.
Protein Expression Results:
ProteinPurified: Not_Tested
ProteinExpressed: Protein_Confirmed
SolubleProtein: Tested_Not_Soluble
Find experimental protocols in NYSGXRC-11151g
Recommended expression in:
Not Applicable