MM_1154 (M. mazei Go1 )
In pSGX3 (His-tagged bacterial expression vector)

Explanation of Terms

Gene:
Gene Symbol:  MM_1154
Gene Name:  NDP-N-acetyl-D-galactosaminuronic acid dehydrogenase
Original Clone ID:
11151g2BCt8p1
Keyword:
full-length cds 3-478 of 478 aa
Species:
Methanosarcina mazei Go1
Type:
cDNA
Vector Name:
pSGX3
Format: 
CLOSED
Source:
New York SGX Research Center for Structural Genomics
Description:
None
Comments:
Linker not verified.
Mutation / Discrepancy:
No / No
Publications:
None
Authors:
PSI
New York SGX Research Center for Structural Genomics
MmCD00519401
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AVAILABLE
No restriction
Special MTA: PSI
Insert sequence: 1455nts
Open reading frame: 1 to 1455

AGTATGAGCAAATTAGAAAAACTTTTGAAGGAACGCGGCCCTATAAAAAAGATAGGCGTT
CTTGGCATGGGATATGTAGGAATCCCTGCTGCTGTCCTTTTTGCAGATGCTCCCTGTTTT
GAGAAAGTCCTCGGCTTCCAGCGCAACTCAAAAAGCTCGGGTTATAAAATTGAAATGCTT
AACAGAGGGGAGAGCCCGCTCAAAGGGGAAGAGCCCGGCCTTGAAGAGCTTATTGGAAAG
GTTGTAAAAGCCGGAAAGTTCGAATGCACACCTGATTTTTCAAGGATTTCCGAGCTTGAT
GCAGTTACACTTGCAATTCAGACTCCTTTTGCAAATCCCAAAGACCTGGAACCTGATTTC
AGCGCGCTTATAGATGGTATCAGAAATGTGGGCAAATACCTTAAACCGGGGATGCTTGTG
GTCCTGGAATCCACAATTACTCCCGGGACAACTGAAGGCATGGCAAAACAGATCCTTGAA
GAAGAGTCAGGGCTAAAAGCAGGTGAAGATTTTGCCCTTGCACACGCCCCCGAAAGAGTG
ATGGTAGGCAGGCTTTTAAAGAACATCAGAGAACACGACAGGATCGTGGGCGGAATAGAT
GAAGCAAGCACAAAAAGGGCTGTTGAACTGTACTCCCCTGTGCTCACAGTCGGACAGGTT
ATCCCTATGAGTGCAACAGCGGCTGAGGTCACAAAAACCGCAGAAAACACTTTTCGCGAC
CTTCAGATTGCAGCAATCAACCAGCTCGCCCTTTACTGTGAAGCTATGGGCATAAATGTC
TATGATGTCAGGACAGGAGTCGACAGCTTAAAAGGCGAGGGAATTACAAGGGCTGTCCTC
TGGCCCGGAGCAGGAGTTGGAGGCCACTGCCTGACCAAAGACACTTACCACCTTGAAAGA
GGAGTTAAAATCGGGAGGGGAGAACTTGACTATCCGGAAGGTGCAGATTCTATTTACGTG
CTTGCAAGGAAAGTCAATGATTTCATGCCCGCACACATGTATAACCTGACCGTTGCAGCC
CTTGAAAGGCTCGGGAAGAAGATGGATGGCTCAAAAGTCGCAATGCTCGGCTGGGCATTT
ATCAAAGATTCGGACGATGCCAGAAACACACCTTCGGAACCATACAGGGACCTCTGCCTG
AAAGCCGGGGCCAGCGTAATGGTACACGACCCTTACGTTGTCAATTATCCGGGCGTTGAA
ATTTCAGATAATCTGGAAGAGGTTGTCAGAAACGCAGACGCTATAGTTGTACTTGCAGGG
CACAGTGCATACTCCAGCCTTAAAGCTGATTGGGCAAAGAAAGTAAGCGCAAAAGCAAAT
CCGGTTATTATTGATGGGAGAAATGTCATCGAGCCGGACGAATTTATCGGGAAAGGGTTC
GTTTACAAGGGAATTGGGAGGGGGGACAAAAACTCACATAAAATAAAAGAAGGGCATCAT
CACCATCACCACTGA

Protein sequence predicted based on nucleotide sequence

SMSKLEKLLKERGPIKKIGVLGMGYVGIPAAVLFADAPCFEKVLGFQRNSKSSGYKIEML
NRGESPLKGEEPGLEELIGKVVKAGKFECTPDFSRISELDAVTLAIQTPFANPKDLEPDF
SALIDGIRNVGKYLKPGMLVVLESTITPGTTEGMAKQILEEESGLKAGEDFALAHAPERV
MVGRLLKNIREHDRIVGGIDEASTKRAVELYSPVLTVGQVIPMSATAAEVTKTAENTFRD
LQIAAINQLALYCEAMGINVYDVRTGVDSLKGEGITRAVLWPGAGVGGHCLTKDTYHLER
GVKIGRGELDYPEGADSIYVLARKVNDFMPAHMYNLTVAALERLGKKMDGSKVAMLGWAF
IKDSDDARNTPSEPYRDLCLKAGASVMVHDPYVVNYPGVEISDNLEEVVRNADAIVVLAG
HSAYSSLKADWAKKVSAKANPVIIDGRNVIEPDEFIGKGFVYKGIGRGDKNSHKIKEGHH
HHHH*

Coding Sequence Details

Insert Sequence Verified?:
Y
Verification Method:
Sequence Verification
Mutations:
None
Discrepancy:
g8c,S3T;a10c,a12t,K4H;
t712n;g1193t,G398V
                                                    

5' Linker Sequence:
None
3' Linker Sequence:
None

Recommended Growth Condition:

Distributed in bacterial strain:
DH5-alpha T1 phage resistant
Antibiotic Selection:
Host Type:  bacterial    Marker:  kanamycin
Bacterial Selection Condition: 50ug/mL kanamycin
Growth Condition: Growth with the single antibiotic in LB at 37 degrees is recommended.
Comments: Conditions for Gateway-type vectors in recombined (with insert) form and other kanamycin resistant vectors.
Protein Expression Results:
ProteinExpressed: Protein_Confirmed
ProteinPurified: Protein_Purified
SolubleProtein: Protein_Soluble
Find experimental protocols in NYSGXRC-11151g
Recommended expression in:
Not Applicable