AT2G13840 (A. thaliana Columbia )
In pDONR221 (Gateway donor/master vector)

Explanation of Terms

Gene:
Gene Symbol:  AT2G13840
Gene Name:  polymerase/histidinol phosphatase-like protein
Original Clone ID:
5566.1.12_GO.8762
Keyword:
full-length cds (mutant)
Species:
Arabidopsis thaliana Columbia
Type:
cDNA
Vector Name:
pDONR221
Format: 
CLOSED
Source:
Center for Eukaryotic Structural Genomics
Description:
None
Comments:
None
Mutation / Discrepancy:
No / Yes
Publications:
None
Authors:
University of Wisconsin Madison
PSI
Center for Eukaryotic Structural Genomics
AtCD00519427
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AVAILABLE
No restriction
Special MTA: PSI
Insert sequence: 1320nts
Open reading frame: 28 to 1347

CCAACTTTGTACAAAAAAGCAGGCTCCGAAAACCCGTACTTCCAGTCCGCCGACGATAAG
AAGAAGCGGAACAAGAAGAAGCGTAGTACCGGTAACAAGAGGAAGATGACCACTGAGCAA
TCAGAAGCCTTTAAGTCAATCACCGATTGGCTCATCTTAGGTTCTTCTCCATCTCTCTCC
TCTTCCTCTGATGACTTTGCCGTCAACATCAATTCTGGTTCTCTAAGATGTGGAGAGAAA
GTTGTGTTTGAGCTTCATTCTCATTCAAATCGCAGCGATGGGTTTCTATCACCTTCAAAG
GTCGTGGAGAGGGCTTATAACAATGGGGTGAAAGTTCTTTCTTTAACGGATCATGATACT
ATGGCTGGTGTCCCGGAAGCAGTGGAAGCAGGACGTAGATTCGGTATCAAGATCATTCCG
GGTATTGAGATCAGCACATTATTCGGTTTAAGAGACTCCGGGTCTGAGGAGCCAGTGCAT
ATACTTGCGTATTATGGCACTTCCGGTCCAGCATTATATGATGAGTTGGAAGATTTCTTG
GTGAAGATAAGAGATGGGCGTTTCGTTCGTGGGAGAGAAATGGTATTGAAATTGAATAAG
CTAAAGATACCTTTAAAATGGGAGCATGTTACCAGGATTGCAGGCAAGGATGTTGCCCCG
GGTAGGATGCATGTAGCCCGAGCGCTTCTTGAAGCAGGCTACGTGGAGAATTTGAGACAA
GCTTTCACTAAATATTTACATGATGGTGGACCTGCTTATGCTACAGGGACTGAGCCTATG
GCCGAGGAAGCAGTAAAACTTATATGCAAAACCGGTGGTGTAGCTGTGCTTGCACATCCG
TGGGCGTTGAAGAATCATGTCGGTATTATTAGGAGATTGAAGGATGCTGGACTTCATGGG
GTCGAGGTTTATAGGAGCGACGGCAAGTTAGAAGTGTTTAGCGAGTTGGCTGATACATAC
AATCTGCTAAAGCTAGGAGGATCAGATTATCATGGAAAAGGCGGACGCAATGAATCAGAA
TTGGGGAGTGTAAACCTTCCCGTGACGGCATTGCAGGACTTCTTGAACGTTGGACGTCCT
ATATGGTGTGAGGCCATTAAAGCAACCATGAGAGCATTCCTTGACCAACCATCAGATTCA
AACCTCTCCAATATCTTAAGGTTCGATAAGGCAAGGATTCTGAAAGGAAATTCAGCTTGG
TCCTGTGGTAAGGAGTTGATGGACCGATGTTTAGCGATTTGGTTGACCAGTGATGAGAGA
AACAGCAATGACTTTGAGGCTCTCAGATTGAAGCTCTCTTTTGTACCAATCATGTCAAAC
GGAACATGTGTCACAGTTGGTCCTTAATAGGACC

Protein sequence predicted based on nucleotide sequence

ENPYFQSADDKKKRNKKKRSTGNKRKMTTEQSEAFKSITDWLILGSSPSLSSSSDDFAVN
INSGSLRCGEKVVFELHSHSNRSDGFLSPSKVVERAYNNGVKVLSLTDHDTMAGVPEAVE
AGRRFGIKIIPGIEISTLFGLRDSGSEEPVHILAYYGTSGPALYDELEDFLVKIRDGRFV
RGREMVLKLNKLKIPLKWEHVTRIAGKDVAPGRMHVARALLEAGYVENLRQAFTKYLHDG
GPAYATGTEPMAEEAVKLICKTGGVAVLAHPWALKNHVGIIRRLKDAGLHGVEVYRSDGK
LEVFSELADTYNLLKLGGSDYHGKGGRNESELGSVNLPVTALQDFLNVGRPIWCEAIKAT
MRAFLDQPSDSNLSNILRFDKARILKGNSAWSCGKELMDRCLAIWLTSDERNSNDFEALR
LKLSFVPIMSNGTCVTVGP*

Coding Sequence Details

Insert Sequence Verified?:
Y
Verification Method:
Sequence Verification
Mutations:
None
Discrepancy:
t8c,L3P;t24c,A8A;t63c,T21T
                                                    

5' Linker Sequence:
None
3' Linker Sequence:
None

Recommended Growth Condition:

Distributed in bacterial strain:
DH5-alpha T1 phage resistant
Antibiotic Selection:
Host Type:  bacterial    Marker:  kanamycin
Bacterial Selection Condition: 50ug/mL kanamycin
Growth Condition: Growth with the single antibiotic in LB at 37 degrees is recommended.
Comments: Conditions for Gateway-type vectors in recombined (with insert) form and other kanamycin resistant vectors.
Protein Expression Results:
ProteinExpressed: Not_Applicable
ProteinPurified: Not_Applicable
SolubleProtein: Not_Applicable
Find experimental protocols in GO.8762
Recommended expression in:
Not Applicable