Gene:
Gene Symbol:
fog-3
Gene Name:
Protein FOG-3
Original Clone ID:
5981.1.15_GO.97569
Keyword:
full-length cds
Species:
Caenorhabditis elegans
Type:
cDNA
Vector Name:
pVP68K
Format:
CLOSED
Source:
Center for Eukaryotic Structural Genomics
Description:
None
Comments:
Linker not verified.
Mutation / Discrepancy:
No / No
Publications:
None
Authors:
University of Wisconsin Madison
PSI
Center for Eukaryotic Structural Genomics
PSI
Center for Eukaryotic Structural Genomics
CeCD00519435
Price:
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AVAILABLE
No restriction
Special MTA: PSI
Special MTA: PSI
Insert sequence: 819nts
Open reading frame: 51 to 869
CTTTAATTAACGGCGACGGTGCCGGGCTGGAAGTTCTGTTCCAGGGGCCCGCGATCGCCG
AAAACCTGTACTTCCAGTCCTATACCGAAGTCCGCGAGCTCGTCAATTTTGTGTGCAGAT
ACTTATTTGGACATATTCCAAGAAGACCGGTCGGCATATTTGGCGCTGAACTTGGAAACT
ACCTCGTCTCACATTTCTCGTCTACCTGGGATGTTCATCATCCGAAAAATGGTGAGATGA
AGCGAATGATTAACACAACCACTTCTTTATGCTTTGCAAGTTCTGCCGAAGAAGCTGGAG
TCCCTCCGAGTGATGTTCTGCGTCTACTTCCAACAAACATGATCATTTTTGCCAATCCGG
GCCACGTCTTCGTTCGTTTATCGGAGAACGGAATCGAAACACCGATTTGGATTGGAGATG
TGAACTGCGATGAGAACTACCAATCAGTCCCCGAGTACGTGGTCAGAACCGCTGCAATCC
GCGCGGAGCCTTGCTCGAATCTTGGAGCAGCTGGGAAATCGGTTCTTGTTGGAAAAAAGC
CGCTTCTCACCAACGACAAAGCTGCTCTCGAAATGGTCAACACAATGTATTCTCCATTGG
CGCGAGAGAAATGTGATGATATCAACGCAAATCTCTCTCATCTTCGGGAGATGTATCCAT
TCCGCTTCGTCTACAAGCCGTCATCTGCTCAAACCTTCAGTGGCGTTGAGTTCTCACAGA
CTCGTTTCGGATCTAGCAAATCTCGTCCGGATCTTCAGACAATGGATGTCATCAAGTACC
TCAGCTCTCAGCAAGTTTCGTCTTCGTCTTCGCCAAGCTTCACCTATTCAAGCATCAACG
ACCAAATGAGATATTCTCCCCGTTTTTAA
Protein sequence predicted based on nucleotide sequence
AIAENLYFQSYTEVRELVNFVCRYLFGHIPRRPVGIFGAELGNYLVSHFSSTWDVHHPKN GEMKRMINTTTSLCFASSAEEAGVPPSDVLRLLPTNMIIFANPGHVFVRLSENGIETPIW IGDVNCDENYQSVPEYVVRTAAIRAEPCSNLGAAGKSVLVGKKPLLTNDKAALEMVNTMY SPLAREKCDDINANLSHLREMYPFRFVYKPSSAQTFSGVEFSQTRFGSSKSRPDLQTMDV IKYLSSQQVSSSSSPSFTYSSINDQMRYSPRF*
Coding Sequence Details
Insert Sequence Verified?:
Y
Verification Method:
Sequence Verification
Mutations:
None
Discrepancy:
None
5' Linker Sequence:
None
3' Linker Sequence:
None
Recommended Growth Condition:
Distributed in bacterial strain:
DH5-alpha T1 phage resistant
Antibiotic Selection:
Host Type:
bacterial
Marker:
kanamycin
Bacterial Selection Condition:
50ug/mL kanamycin
Growth Condition:
Growth with the single antibiotic in LB at 37 degrees is recommended.
Comments:
Conditions for Gateway-type vectors in recombined (with insert) form and other kanamycin resistant vectors.
Protein Expression Results:
ProteinExpressed: Protein_Confirmed
SolubleProtein: Protein_Soluble
ProteinPurified: Tested_Not_Purified
Find experimental protocols in GO.97569
Recommended expression in:
Not Applicable
Vector Name:
pVP68K
Synonyms:
''
Sequencing Primer:
Forward:
MBP Forward
Reverse:
T7 Terminator
Description:
Bacterial (E. coli) expression with N-terminal 8xHis, MBP tag; kanamycin resistance in bacteria; restriction enzyme cloning (SgfI, PmeI); Cloning into this vector will remove the BarCat cassette.
Comments:
Vector contains a lethal BarCat cassette that requires the strain BR610 for growth. Lethal cassette is removed during cloning.
Size (bp):
6353
Parent Vector:
None
Properties:
bacterial expression
Author Name:
University of Wisconsin
Center for Eukaryotic Structural Genomics
Center for Eukaryotic Structural Genomics
Publications:
PMID:
18988018
Title: Flexi vector cloning.
Vector Map:
Vector Sequence:
Sequence:
Vector Features:
Type
Name
Description
Start
End
bacterial origin
pBR322 origin
pBR322 bacterial origin of replication
4150
5377
gene fragment
Barnase
Barnase
1448
1780
primer
MBP forward
MBP forward primer site
1271
1291
primer
T7 term
T7 terminator reverse primer
2599
2616
promoter
T5
T5 promoter region
122
202
protease cleavage site
3C
3C protease cleavage site
1389
1412
repressor binding site
LacI
LacI coding region
2980
4071
selectable marker
KanR
Kanamycin resistance gene
5385
6179
selectable marker
CmR
chloramphenicol acetyltransferase gene
1885
2544
tag
His
N-terminal 8xHis tag
237
260
tag
MBP
N-terminal Maltose Binding Protein tag
267
1363
trxn termination sequence
rrnB
rrnB transcription terminator
2796
2892
