AT4G16580 (A. thaliana Columbia )
In pDONR221 (Gateway donor/master vector)

Explanation of Terms

Gene:
Gene Symbol:  AT4G16580
Gene Name:  putative protein phosphatase 2C
Original Clone ID:
6002.1.84_GO.20649
Keyword:
full-length cds (mutant)
Species:
Arabidopsis thaliana Columbia
Type:
cDNA
Vector Name:
pDONR221
Format: 
CLOSED
Source:
Center for Eukaryotic Structural Genomics
Description:
None
Comments:
None
Mutation / Discrepancy:
No / No
Publications:
None
Authors:
University of Wisconsin Madison
PSI
Center for Eukaryotic Structural Genomics
AtCD00519457
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AVAILABLE
No restriction
Special MTA: PSI
Insert sequence: 1422nts
Open reading frame: 17 to 1438

CAAAAAAGCAGGCTCCGAAAACCCGTACTTCCAGTCCTTACCCGTGAGAGAAAGTCTTCA
GAAACAAGTTAAGATTTTGATCGGATTAGGGAATTTAGGGTTTGGTGGTTATAGAGGATT
GTACACTAGATTTACTAACCCTAACGGGTTTTTAGAACCAGCTAGCTCTGATTTGTTGTT
GATTAACGAAAGGAGGAATCTTTCTGTTATTGGTGCTGTTTCTCGAACTTTCTCTGTTCC
TTCTGTATCTGGTCCGGCCTTTCAGGTCTGTGGATATCACATTGATCTTTTGCTTTCGGA
TCCGTGTAAATCAATGGCGAGTTTAGGTTCTAAGTCTTTGTTTGTGGATCGTCATTCTGC
TTCTCTTGTTTCAAAGCGTTTCACTGGTGGTATGGTATCTGGAGATGGTCCTAACCGTGG
AAGAATCAGTATGAGACTCAGAGGTAAAGATCACAATGAGAAGTCTACGATCTGTGCGTA
TTTTGCTTATAGAGGTGCTAAGAGATGGATTTATTTGAATCAGCAAAGAAGAGGAATGGG
ATTTAGAGGCTTGCATAGCTCACTCTCTAATCGCTTATCAGCTGGGAATGCGCCTGATGT
CTCTTTAGATAACTCTGTTACTGACGAACAGGTTAGAGATTCTTCGGATTCTGTAGCGGC
TAAGCTATGTACTAAGCCTCTGAAGCTTGTCTCGGGGTCGTGTTATCTACCGCATCCTGA
TAAAGAGGCCACTGGGGGAGAGGATGCACATTTTATCTGTGCTGAAGAGCAAGCATTAGG
TGTGGCAGATGGTGTGGGGGGTTGGGCAGAGCTTGGTATTGATGCAGGTTACTATTCTCG
GGAGCTTATGTCTAACTCAGTGAATGCAATCCAAGATGAGCCTAAAGGTTCGATTGATCC
AGCCAGAGTATTGGAAAAAGCTCATACTTGCACAAAGTCGCAAGGGTCTTCAACAGCTTG
CATCATAGCCTTAACCAACCAGGGACTTCATGCAATCAATTTAGGCGACAGCGGGTTCAT
GGTTGTTCGTGAAGGGCATACGGTTTTCCGTTCCCCTGTTCAACAGCATGACTTCAACTT
CACCTATCAGCTGGAGAGTGGAAGAAACGGCGATTTGCCAAGCTCAGGTCAGGTTTTCAC
TGTTGCTGTCGCTCCTGGAGACGTCATAATAGCTGGAACAGATGGGTTGTTTGACAACCT
GTACAACAACGAGATCACAGCCATAGTGGTTCACGCTGTGAGAACCAACATAGATCCCCA
GGTAACAGCGCAGAAGATTGCAGCATTGGCGCGACAAAGAGCACAGGACAAAAACAGACA
AACTCCATTCTCGACAGCAGCACAAGATGCTGGCTTCAGATACTACGGTGGTAAGCTTGA
TGACATTACAGTTGTTGTCTCTTATGTGGCGGCTTCGAAAGAAGAAGGAAAACATTGATA
GGACC

Protein sequence predicted based on nucleotide sequence

ENPYFQSLPVRESLQKQVKILIGLGNLGFGGYRGLYTRFTNPNGFLEPASSDLLLINERR
NLSVIGAVSRTFSVPSVSGPAFQVCGYHIDLLLSDPCKSMASLGSKSLFVDRHSASLVSK
RFTGGMVSGDGPNRGRISMRLRGKDHNEKSTICAYFAYRGAKRWIYLNQQRRGMGFRGLH
SSLSNRLSAGNAPDVSLDNSVTDEQVRDSSDSVAAKLCTKPLKLVSGSCYLPHPDKEATG
GEDAHFICAEEQALGVADGVGGWAELGIDAGYYSRELMSNSVNAIQDEPKGSIDPARVLE
KAHTCTKSQGSSTACIIALTNQGLHAINLGDSGFMVVREGHTVFRSPVQQHDFNFTYQLE
SGRNGDLPSSGQVFTVAVAPGDVIIAGTDGLFDNLYNNEITAIVVHAVRTNIDPQVTAQK
IAALARQRAQDKNRQTPFSTAAQDAGFRYYGGKLDDITVVVSYVAASKEEGKH*

Coding Sequence Details

Insert Sequence Verified?:
Y
Verification Method:
Sequence Verification
Mutations:
None
Discrepancy:
t8c,L3P;t27c,P9P;t66c,I22I
                                                    

5' Linker Sequence:
None
3' Linker Sequence:
None

Recommended Growth Condition:

Distributed in bacterial strain:
DH5-alpha T1 phage resistant
Antibiotic Selection:
Host Type:  bacterial    Marker:  kanamycin
Bacterial Selection Condition: 50ug/mL kanamycin
Growth Condition: Growth with the single antibiotic in LB at 37 degrees is recommended.
Comments: Conditions for Gateway-type vectors in recombined (with insert) form and other kanamycin resistant vectors.
Protein Expression Results:
ProteinExpressed: Not_Applicable
ProteinPurified: Not_Applicable
SolubleProtein: Not_Applicable
Find experimental protocols in GO.20649
Recommended expression in:
Not Applicable