PSMD3 (Homo sapiens) in pDONR221 (Gateway donor/master vector)

Explanation of Terms

Gene:
Gene Symbol:  PSMD3
Gene Name:  proteasome (prosome, macropain) 26S subunit, non-ATPase, 3
Original Clone ID:
5563.1.06_GO.35473
Keyword:
None
Species:
Homo sapiens
Type:
cDNA
Vector Name:
pDONR221
Format: 
CLOSED
Source:
Center for Eukaryotic Structural Genomics
Description:
None
Comments:
None
Mutation / Discrepancy:
No / No
Publications:
None
Authors:
University of Wisconsin Madison
PSI
Center for Eukaryotic Structural Genomics
HsCD00519487
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AVAILABLE
No restriction
Special MTA: PSI
Insert sequence: 1653nts
Open reading frame: 1 to 1653

ATGCCAACTTTGTACAAAAAAGCAGGCTCCGAAAACCTGTACTTCCAGTCCAAGCAGGAG
GGCTCGGCGCGGCGCCGCGGCGCGGACAAGGCGAAACCGCCGCCCGGCGGAGGAGAACAA
GAACCCCCACCGCCGCCGGCCCCCCAGGATGTGGAGATGAAAGAGGAGGCAGCGACGGGT
GGCGGGTCGACGGGGGAGGCAGACGGCAAGACGGCGGCGGCAGCGGCTGAGCACTCCCAG
CGAGAGCTGGACACAGTCACCTTGGAGGACATCAAGGAGCACGTGAAACAGCTAGAGAAA
GCGGTTTCAGGCAAGGAGCCGAGATTCGTGCTGCGGGCCCTGCGGATGCTGCCTTCCACA
TCACGCCGCCTCAACCACTATGTTCTGTATAAGGCTGTGCAGGGCTTCTTCACTTCAAAT
AATGCCACTCGAGACTTTTTGCTCCCCTTCCTGGAAGAGCCCATGGACACAGAGGCTGAT
TTACAGTTCCGTCCCCGCACGGGAAAAGCTGCGTCGACACCCCTCCTGCCTGAAGTGGAA
GCCTATCTCCAACTCCTCGTGGTCATCTTCATGATGAACAGCAAGCGCTACAAAGAGGCA
CAGAAGATCTCTGATGATCTGATGCAGAAGATCAGTACTCAGAACCGCCGGGCCCTAGAC
CTTGTAGCCGCAAAGTGTTACTATTATCACGCCCGGGTCTATGAGTTCCTGGACAAGCTG
GATGTGGTGCGCAGCTTCTTGCATGCTCGGCTCCGGACAGCTACGCTTCGGCATGACGCA
GACGGGCAGGCCACCCTGTTGAACCTCCTGCTGCGGAATTACCTACACTACAGCTTGTAC
GACCAGGCTGAGAAGCTGGTGTCCAAGTCTGTGTTCCCAGAGCAGGCCAACAACAATGAG
TGGGCCAGGTACCTCTACTACACAGGGCGAATCAAAGCCATCCAGCTGGAGTACTCAGAG
GCCCGGAGAACGATGACCAACGCCCTTCGCAAGGCCCCTCAGCACACAGCTGTCGGCTTC
AAACAGACGGTGCACAAGCTTCTCATCGTGGTGGAGCTGTTGCTGGGGGAGATCCCTGAC
CGGCTGCAGTTCCGCCAGCCCTCCCTCAAGCGCTCACTCATGCCCTATTTCCTTCTGACT
CAAGCTGTCAGGACAGGAAACCTAGCCAAGTTCAACCAGGTCCTGGATCAGTTTGGGGAG
AAGTTTCAAGCAGATGGGACCTACACCCTAATTATCCGGCTGCGGCACAACGTGATTAAG
ACAGGTGTACGCATGATCAGCCTCTCCTATTCCCGAATCTCCTTGGCTGACATCGCCCAG
AAGCTGCAGTTGGATAGCCCCGAAGATGCAGAGTTCATTGTTGCCAAGGCCATCCGGGAT
GGTGTCATTGAGGCCAGCATCAACCACGAGAAGGGCTATGTCCAATCCAAGGAGATGATT
GACATCTATTCCACCCGAGAGCCCCAGCTAGCCTTCCACCAGCGCATCTCCTTCTGCCTA
GATATCCACAACATGTCTGTCAAGGCCATGAGGTTTCCTCCCAAATCGTACAACAAGGAC
TTGGAGTCTGCAGAGGAACGGCGTGAGCGAGAACAGCAGGACTTGGAGTTTGCCAAGGAG
ATGGCAGAAGATGATGATGACAGCTTCCCTTGA

Protein sequence predicted based on nucleotide sequence

MPTLYKKAGSENLYFQSKQEGSARRRGADKAKPPPGGGEQEPPPPPAPQDVEMKEEAATG
GGSTGEADGKTAAAAAEHSQRELDTVTLEDIKEHVKQLEKAVSGKEPRFVLRALRMLPST
SRRLNHYVLYKAVQGFFTSNNATRDFLLPFLEEPMDTEADLQFRPRTGKAASTPLLPEVE
AYLQLLVVIFMMNSKRYKEAQKISDDLMQKISTQNRRALDLVAAKCYYYHARVYEFLDKL
DVVRSFLHARLRTATLRHDADGQATLLNLLLRNYLHYSLYDQAEKLVSKSVFPEQANNNE
WARYLYYTGRIKAIQLEYSEARRTMTNALRKAPQHTAVGFKQTVHKLLIVVELLLGEIPD
RLQFRQPSLKRSLMPYFLLTQAVRTGNLAKFNQVLDQFGEKFQADGTYTLIIRLRHNVIK
TGVRMISLSYSRISLADIAQKLQLDSPEDAEFIVAKAIRDGVIEASINHEKGYVQSKEMI
DIYSTREPQLAFHQRISFCLDIHNMSVKAMRFPPKSYNKDLESAEERREREQQDLEFAKE
MAEDDDDSFP*

Coding Sequence Details

Insert Sequence Verified?:
Y
Verification Method:
Sequence Verification
Mutations:
None
Discrepancy:
t8c,L3P
                                                    

5' Linker Sequence:
None
3' Linker Sequence:
None

Recommended Growth Condition:

Distributed in bacterial strain:
DH5-alpha T1 phage resistant
Antibiotic Selection:
Host Type:  bacterial    Marker:  kanamycin
Bacterial Selection Condition: 50ug/mL kanamycin
Growth Condition: Growth with the single antibiotic in LB at 37 degrees is recommended.
Comments: Conditions for Gateway-type vectors in recombined (with insert) form and other kanamycin resistant vectors.
Protein Expression Results:
ProteinExpressed: Not_Applicable
ProteinPurified: Not_Applicable
SolubleProtein: Not_Applicable
Find experimental protocols in GO.35473
Recommended expression in:
Not Applicable