ATP6V1H (Homo sapiens) in pDONR221 (Gateway donor/master vector)

Explanation of Terms

Gene:
Gene Symbol:  ATP6V1H
Gene Name:  ATPase, H+ transporting, lysosomal 50/57kDa, V1 subunit H
Original Clone ID:
5543.1.49_GO.79445
Keyword:
None
Species:
Homo sapiens
Type:
cDNA
Vector Name:
pDONR221
Format: 
CLOSED
Source:
Center for Eukaryotic Structural Genomics
Description:
None
Comments:
None
Mutation / Discrepancy:
No / No
Publications:
None
Authors:
University of Wisconsin Madison
PSI
Center for Eukaryotic Structural Genomics
HsCD00519545
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AVAILABLE
No restriction
Special MTA: PSI
Insert sequence: 1500nts
Open reading frame: 1 to 1500

ATGCCAACTTTGTACAAAAAAGCAGGCTCCGAAAACCTGTACTTCCAGTCCACCAAAATG
GATATCCGAGGTGCTGTGGATGCTGCTGTCCCCACCAATATTATTGCTGCCAAGGCTGCA
GAAGTTCGTGCAAACAAAGTCAACTGGCAATCCTATCTTCAGGGACAGATGATTTCTGCT
GAAGATTGTGAGTTTATTCAGAGGTTTGAAATGAAACGAAGCCCTGAAGAGAAGCAAGAG
ATGCTTCAAACTGAAGGCAGCCAGTGTGCTAAAACATTTATAAATCTGATGACTCATATC
TGCAAAGAACAGACCGTTCAGTATATACTAACTATGGTGGATGATATGCTGCAGGAAAAT
CATCAGCGTGTTAGCATTTTCTTTGACTATGCAAGATGTAGCAAGAACACTGCGTGGCCC
TACTTTCTGCCAATGTTGAATCGCCAGGATCCCTTCACTGTTCATATGGCAGCAAGAATT
ATTGCCAAGTTAGCAGCTTGGGGAAAAGAACTGATGGAAGGCAGTGACTTAAATTACTAT
TTCAATTGGATAAAAACTCAGCTGAGTTCACAGAAACTGCGTGGTAGCGGTGTTGCTGTT
GAAACAGGAACAGTCTCTTCAAGTGATAGTTCGCAGTATGTGCAGTGCGTGGCCGGGTGT
TTGCAGCTGATGCTCCGGGTCAATGAGTACCGCTTTGCTTGGGTGGAAGCAGATGGGGTA
AATTGCATAATGGGAGTGTTGAGTAACAAGTGTGGCTTTCAGCTCCAGTATCAAATGATT
TTTTCAATATGGCTCCTGGCATTCAGTCCTCAAATGTGTGAACACCTGCGGCGCTATAAT
ATCATTCCAGTTCTGTCTGATATCCTTCAGGAGTCTGTCAAAGAGAAAGTAACAAGAATC
ATTCTTGCAGCATTTCGTAACTTTTTAGAAAAATCAACTGAAAGAGAAACTCGCCAAGAA
TATGCCCTGGCTATGATTCAGTGCAAAGTTCTGAAACAGTTGGAGAACTTGGAACAGCAG
AAGTACGATGATGAAGATATCAGCGAAGATATCAAATTTCTTTTGGAAAAACTTGGAGAG
AGTGTCCAGGACCTTAGTTCATTTGATGAATACAGTTCAGAACTTAAATCTGGAAGGTTG
GAATGGAGTCCTGTGCACAAATCTGAGAAATTTTGGAGAGAGAATGCTGTGAGGTTAAAT
GAGAAGAATTATGAACTCTTGAAAATCTTGACAAAACTTTTGGAAGTGTCAGATGATCCC
CAAGTCTTAGCTGTTGCTGCTCACGATGTTGGAGAATATGTGCGGCATTATCCACGAGGC
AAACGGGTCATCGAGCAGCTCGGTGGGAAGCAGCTGGTCATGAACCACATGCATCATGAA
GACCAGCAGGTCCGCTATAATGCTCTGCTGGCCGTGCAGAAGCTCATGGTGCACAACTGG
GAATACCTTGGCAAGCAGCTCCAGTCCGAGCAGCCCCAGACCGCTGCCGCCCGAAGCTAA

Protein sequence predicted based on nucleotide sequence

MPTLYKKAGSENLYFQSTKMDIRGAVDAAVPTNIIAAKAAEVRANKVNWQSYLQGQMISA
EDCEFIQRFEMKRSPEEKQEMLQTEGSQCAKTFINLMTHICKEQTVQYILTMVDDMLQEN
HQRVSIFFDYARCSKNTAWPYFLPMLNRQDPFTVHMAARIIAKLAAWGKELMEGSDLNYY
FNWIKTQLSSQKLRGSGVAVETGTVSSSDSSQYVQCVAGCLQLMLRVNEYRFAWVEADGV
NCIMGVLSNKCGFQLQYQMIFSIWLLAFSPQMCEHLRRYNIIPVLSDILQESVKEKVTRI
ILAAFRNFLEKSTERETRQEYALAMIQCKVLKQLENLEQQKYDDEDISEDIKFLLEKLGE
SVQDLSSFDEYSSELKSGRLEWSPVHKSEKFWRENAVRLNEKNYELLKILTKLLEVSDDP
QVLAVAAHDVGEYVRHYPRGKRVIEQLGGKQLVMNHMHHEDQQVRYNALLAVQKLMVHNW
EYLGKQLQSEQPQTAAARS*

Coding Sequence Details

Insert Sequence Verified?:
Y
Verification Method:
Sequence Verification
Mutations:
None
Discrepancy:
t8c,L3P;t29c,M10T
                                                    

5' Linker Sequence:
None
3' Linker Sequence:
None

Recommended Growth Condition:

Distributed in bacterial strain:
DH5-alpha T1 phage resistant
Antibiotic Selection:
Host Type:  bacterial    Marker:  kanamycin
Bacterial Selection Condition: 50ug/mL kanamycin
Growth Condition: Growth with the single antibiotic in LB at 37 degrees is recommended.
Comments: Conditions for Gateway-type vectors in recombined (with insert) form and other kanamycin resistant vectors.
Protein Expression Results:
ProteinExpressed: Not_Applicable
ProteinPurified: Not_Applicable
SolubleProtein: Not_Applicable
Find experimental protocols in GO.79445
Recommended expression in:
Not Applicable