Aaci_0447 (A. acidocaldarius subsp. acidocaldarius DSM 446 )
In pMCSG48

Explanation of Terms

Gene:
Gene Symbol:  Aaci_0447
Gene Name:  hypothetical protein
Original Clone ID:
APC100350.104
Keyword:
None
Species:
Alicyclobacillus acidocaldarius subsp. acidocaldarius DSM 446
Type:
cDNA
Vector Name:
pMCSG48
Format: 
CLOSED
Source:
Midwest Center for Structural Genomics
Description:
None
Comments:
None
Mutation / Discrepancy:
No / No
Publications:
None
Authors:
Jessica Bearden
PSI:Biology
Argonne National Laboratory
Midwest Center for Structural Genomics
AaCD00519751
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AVAILABLE
No restriction
Special MTA: PSI
Insert sequence: 1245nts
Open reading frame: 50 to 1294

CCCGAGATGGCGCCGTATACACACGAGAACTGTACTTCCATCCAATGCCGTGACCGGTCG
CGACAAGCTCACGCTCGCGGACATCCGGCCGTTGGCGGAAGAGGTGGGGCTTCCGGAAAT
CGCGGCGACCGACGCGTCTCCGTTTGACGATCTCGCCCGCCCACTCGCGGACTACGAGGC
GCGCGGGCGCACGGGCTTCGAGTGGGCCGATGCGGCGGAGCGGATGGATCCGCGCTTTTG
GCTGCCCGAGGCGAAGTCCATCCTGACGGCGGCGCTCCCTTATCTCACTGAAGAGGGCGC
GCGGCGGATGCGGGAACACCCGCGATCCGAGATCTGCGGCCAAACCTCGTGTTACGTGTA
TGGCGAAGACTATCACCGCGTGCTCTCCGATCGCCTGACCGCGCTGGCGGGGCGCATCGG
GAACCTCGTCGGGCGCGAGATCCGGTACCGGGTGGCGGTCGACACCTCTCCGCTCGTCGA
TCGCCGCGTCGCCGAGCGCAGCGGGCTTGGCTGGATCGGCAAAAACGGCATGCTGTTCAG
CCCACGGTACGGATCGTACGTCTTCCTCGGGGCGCTGCTCATCGACGTCGAGATCGAAGA
CGCGTTGCACCTCCCCCCGGCCATCGGCAAGCACTGCGGCGACTGCGATCTCTGCATGCG
TGCGTGCCCCACGCAGGCCTTCGTCGCGCCGGGCGAACTGAAGGCGACCGCCTGCTTGTC
GTACGTGACGCAGATGAAGGGGATCATTCCTCGCGCTTATCGCAAACCGCTCGGTCGGCG
CGTGTGGGGCTGCGACGTGTGCCAGCAGGTGTGCCCGCTGAATCGAGATCGTGCCCACGC
TGAGGATCCCGCGTTCACCCCTGATGCCGAAGCCGCGTATCCCGATCTCGTCAGGCTGCT
CGGGATGTCGAATCGCCAGTTCTTGCGCGCCTACGGCCGTTCGGCCATGGCGTGGCGAGG
CGTGAGAACGTTGCAGCGGAATGCGCTCGTGGCCCTCGGCAATCTGCGCCGCCCGGAAGC
CGTGCCGATCGTGGCGCGATTTTTGGCGTCGGATCGGGTGGAGTTGCGCGCAAGCGCCGC
ATGGGCGCTGGGCGAGATCGGCGGACAGGATGCGCTCCGCGAGCTTCGAGCTGCCTTCGC
TTCGGAACAGGATCCGGACGTGCGGAAGGAGATGGAGGATGCCATCGCGAATGCCTGGGG
CAAAGCCTGCGAAGGAGACGAGGGACGTGATGAGACGGGATCGGGCTATCGCGACTGTGA
CCACCCCTGTCGGGGAGATGTGGCTGGTCGCTAACATTGGAAGTGGATAATAA

Protein sequence predicted based on nucleotide sequence

VTGRDKLTLADIRPLAEEVGLPEIAATDASPFDDLARPLADYEARGRTGFEWADAAERMD
PRFWLPEAKSILTAALPYLTEEGARRMREHPRSEICGQTSCYVYGEDYHRVLSDRLTALA
GRIGNLVGREIRYRVAVDTSPLVDRRVAERSGLGWIGKNGMLFSPRYGSYVFLGALLIDV
EIEDALHLPPAIGKHCGDCDLCMRACPTQAFVAPGELKATACLSYVTQMKGIIPRAYRKP
LGRRVWGCDVCQQVCPLNRDRAHAEDPAFTPDAEAAYPDLVRLLGMSNRQFLRAYGRSAM
AWRGVRTLQRNALVALGNLRRPEAVPIVARFLASDRVELRASAAWALGEIGGQDALRELR
AAFASEQDPDVRKEMEDAIANAWGKACEGDEGRDETGSGYRDCDHPCRGDVAGR*

Coding Sequence Details

Insert Sequence Verified?:
Y
Verification Method:
Sequence Verification
Mutations:
None
Discrepancy:
None
5' Linker Sequence:
None
3' Linker Sequence:
None

Recommended Growth Condition:

Distributed in bacterial strain:
DH5-alpha T1 phage resistant
Antibiotic Selection:
Host Type:  bacterial    Marker:  ampicillin
Bacterial Selection Condition: 100 ug/mL ampicillin
Growth Condition: Growth with the single antibiotic in LB at 37 degrees is recommended.
Comments: Commonly used conditions for ampicillin resistant plasmid clones.
Protein Expression Results:
ProteinPurified: Not_Tested
ProteinExpressed: Protein_Confirmed
SolubleProtein: Protein_Soluble
Find experimental protocols in MCSG-APC100350
Recommended expression in:
Not Applicable