Plim_0812 (P. limnophila )
In pMCSG7 (His-tagged bacterial expression vector)

Explanation of Terms

Gene:
Gene Symbol:  Plim_0812
Gene Name:  stage II sporulation E family protein
Original Clone ID:
APC100656.102
Keyword:
None
Species:
Planctopirus limnophila
Type:
cDNA
Vector Name:
pMCSG7
Format: 
CLOSED
Source:
Midwest Center for Structural Genomics
Description:
None
Comments:
None
Mutation / Discrepancy:
No / No
Publications:
None
Authors:
Jessica Bearden
PSI:Biology
Midwest Center for Structural Genomics
PlCD00519795
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AVAILABLE
No restriction
Special MTA: PSI
Insert sequence: 1269nts
Open reading frame: 51 to 1319

CTTCTGGTGTAGATCTGGGTACCGAGAACCTGTACTTCCAATCCAATGCCATGCTTTCGG
CCCCCCATCTGCCCAACGAAGAGCAGCGAATCCAGGAGTTGTATGCCCTGGAACTGCTGG
ACACCCCGCCCGAAGACCGCTACGACTCAATCATTCGTGCCTTAAGAGCCGTCTACAAGG
TTCCGATCGCTTACATTTCGCTGGTCGATCGTGATCGACAATGGTTCAAGGCTCGGGTCG
GCCTCGAAATTCTCGAAACACCTCGCAGTATCTCATTTTGTGGCCATGCAATTGCTCAGC
AGGAACCACTGGTTGTTCCTGATCTGACGCAAGATAGCCGCTTTGCCGATAACCCGATGG
TGCTGGAAGCCCCTCATTTGAGGTTCTATGCCGGTCTTCGCCTCTCAGGTCCGACCGGGC
AGCCCGTGGGGACACTTTGCATGGCGGATACGGTCCCCTTTGAGAAGCCATTTGATGATG
CACCACTCAAAGAACTGGCCCATCTCGTCGAGCAGCAGTTTCAAATGCTCGATCTGATTT
CGGCTCAGAAAAAGCTCCTCGAAGTCCGTGCCCAACTCCTACAGACTCAGGCTTTACTGC
AGAATGAGTTGCGAGATGCGGCAGAATACATTCACTCGCTGCTTCCGACCCCGCTGACGG
AATCAATCTCCACCAGTCACGAGCTGGTGGCCTGCTCGGAACTGGGTGGAGATCTCTTCG
GCTATCACTGGATCAGTGAAGAAGAACTGGCGATTTACCTGCTCGATGTGAGTGGGCATG
GAGTCGGAGCATCACTGCTTTCCGTTTCTGCCCTGAATGCACTCCGCAGACAGGCCTTGC
CCGGGATCTGCTTTACCGATGTCGAATCGGTGCTGTGCGCCATGAATTCAGCATTCCCGA
TGGAAGAGCACAATGGAAAGTTTCTCACCTTGTGGTACGGAGTGTTTCACACAGGACGAC
GAGAACTGGAGTATGGCGCAGCAGGTCATCCACCCGCCCTGTTGTGGCAACGTCCCGGCG
GGCCCTGCACAAAGCTCGTTTCCAATCAGATCATGATCGGAGCGGCAGCCGACTTTCCTT
ATCAGGCAGAACGCGTGCGAGTTCAGCAAGGGGCCAGGCTCTATGTTTACAGCGATGGTG
CCTTTGAACTGGATTCACCAGCAGGTGAAAGTCTGGGGATTCAAGGCTTAATCCAAACCA
TCGAGAAAAATGCCCACCGATCCAGCCAGCGAGTATCAGCCATTCGAGAATCGCTGCAAG
CCTTCCAGGGATCGGCAGATTTTGTGGACGACTTTTCCATGATTGAACTCTGCTTGTAAC
ATTGGAAGTGGATAATAA

Protein sequence predicted based on nucleotide sequence

MLSAPHLPNEEQRIQELYALELLDTPPEDRYDSIIRALRAVYKVPIAYISLVDRDRQWFK
ARVGLEILETPRSISFCGHAIAQQEPLVVPDLTQDSRFADNPMVLEAPHLRFYAGLRLSG
PTGQPVGTLCMADTVPFEKPFDDAPLKELAHLVEQQFQMLDLISAQKKLLEVRAQLLQTQ
ALLQNELRDAAEYIHSLLPTPLTESISTSHELVACSELGGDLFGYHWISEEELAIYLLDV
SGHGVGASLLSVSALNALRRQALPGICFTDVESVLCAMNSAFPMEEHNGKFLTLWYGVFH
TGRRELEYGAAGHPPALLWQRPGGPCTKLVSNQIMIGAAADFPYQAERVRVQQGARLYVY
SDGAFELDSPAGESLGIQGLIQTIEKNAHRSSQRVSAIRESLQAFQGSADFVDDFSMIEL
CL*

Coding Sequence Details

Insert Sequence Verified?:
Y
Verification Method:
Sequence Verification
Mutations:
None
Discrepancy:
None
5' Linker Sequence:
None
3' Linker Sequence:
None

Recommended Growth Condition:

Distributed in bacterial strain:
DH5-alpha T1 phage resistant
Antibiotic Selection:
Host Type:  bacterial    Marker:  ampicillin
Bacterial Selection Condition: 100 ug/mL ampicillin
Growth Condition: Growth with the single antibiotic in LB at 37 degrees is recommended.
Comments: Commonly used conditions for ampicillin resistant plasmid clones.
Protein Expression Results:
ProteinExpressed: Protein_Confirmed
ProteinPurified: Protein_Purified
SolubleProtein: Protein_Soluble
Find experimental protocols in MCSG-APC100656
Recommended expression in:
Not Applicable