Hmuk_3119 (H. mukohataei DSM 12286 )
In pMCSG7 (His-tagged bacterial expression vector)

Explanation of Terms

Gene:
Gene Symbol:  Hmuk_3119
Gene Name:  von Willebrand factor A
Original Clone ID:
APC101199.102
Keyword:
None
Species:
Halomicrobium mukohataei DSM 12286
Type:
cDNA
Vector Name:
pMCSG7
Format: 
CLOSED
Source:
Midwest Center for Structural Genomics
Description:
None
Comments:
None
Mutation / Discrepancy:
No / Yes
Publications:
None
Authors:
Jessica Bearden
PSI:Biology
Midwest Center for Structural Genomics
HmCD00519885
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AVAILABLE
No restriction
Special MTA: PSI
Insert sequence: 1182nts
Open reading frame: 48 to 1229

CTGGTGTAGATCTGGGTACCGAGAACCTGTACTTCCAATCCAATGCCATGGCATCTATCG
AGACGAGCGTCAATCGGCCGAACGTACCGGCCGACGGCACGACCGTGACCGCCGAGATCG
ACGTCGAGCCGGGAGAACAGGAGACGGACGTGCGACGCCACATCGCGCTCTGTATCGACA
CGAGCGGGTCGATGGAGGGTGACAACATCAAACGCGCTCGCGACGGCGCTGCGTGGGTCT
TCGGGCTGTTGGCCGACGAGGACTACGTGAGTATCGTCGCGTTCGACACCGAGGCGACGG
TGATCCTGCCCGCGACACGGTGGTCGGATCTCGACCGCCAGACGGCGATGGACCACGTCG
AGGAGCTGACTGCCGGCGGCGGCACCGACATGTACAACGGGCTCAAGGCCGCCAAGGAGA
CGCTGTCGTCCTCCGCGACCGGGCCCGACACGGTCAAGCGACTCCTCTTGCTCTCGGACG
GCAAGGACAACGAACGCACGCCCGACGAGTTCGAGGGGCTGGCCGAAGCCATCGACGACG
CCGGGATCCGGATCCAGTCGGCCGGGATCGGGACCGACTACAACGAGGCCACGATCCGGA
CGCTCGGGACGGCCGGGCGCGGGACGTGGACCCACCTCGAAGCGCCCGGCGACATCGAGG
ACTTCTTCGGCGAGGCCGTCGAGCAGGCCGGCTCCGTCGTCGCGCCGGACGCCCACCTCG
ACCTCGACGTGGCCCCCGGCGTCGAGGTCAGCGAGGTGTATCGCGCGCTCCCGCAGGCCC
AGGAAGTCTCGCCCGAGTGGGAGGCAAACGCCACCCGGGTCAAGCTCCCCGACCTGATCG
AACGGGAGAGCCAGCGGGTCGTCCTCAAGATCCACGCGCCGCCCCGCGAGCCCGGCAGCG
AGGAGGTGCTCGCGGACGTACAGCTCTCGGCCCGCGGCGACACCGCCAGCGACCAGATCG
GCGTCGAGTACACGGACGAACAGGAGAAGCTGGCCGAGCACAACGAGTCCGTCGACATCG
ACCACAAACAGACCGTCATCCGGACGGAGCTCGGCAAGGGCAACGTTGAGGCCGCAGAGA
CGAAAGTCGAGCAGATGACAGTGATCCACGGCGAGGACGCCGAGGCGGTCCAGGAGGCCG
AGCGCCAGACCGAGATCGTCAAAGAGGGCGGTCGTGCCGAACAGAGCCAGGCGACCCAGA
TCGTCGACAGCGACGACGGCATCCAGTAACATTGGAAGTGGATAATAA

Protein sequence predicted based on nucleotide sequence

MASIETSVNRPNVPADGTTVTAEIDVEPGEQETDVRRHIALCIDTSGSMEGDNIKRARDG
AAWVFGLLADEDYVSIVAFDTEATVILPATRWSDLDRQTAMDHVEELTAGGGTDMYNGLK
AAKETLSSSATGPDTVKRLLLLSDGKDNERTPDEFEGLAEAIDDAGIRIQSAGIGTDYNE
ATIRTLGTAGRGTWTHLEAPGDIEDFFGEAVEQAGSVVAPDAHLDLDVAPGVEVSEVYRA
LPQAQEVSPEWEANATRVKLPDLIERESQRVVLKIHAPPREPGSEEVLADVQLSARGDTA
SDQIGVEYTDEQEKLAEHNESVDIDHKQTVIRTELGKGNVEAAETKVEQMTVIHGEDAEA
VQEAERQTEIVKEGGRAEQSQATQIVDSDDGIQ*

Coding Sequence Details

Insert Sequence Verified?:
Y
Verification Method:
Sequence Verification
Mutations:
None
Discrepancy:
c1020t,V340V;g1029a,A343A
                                                    

5' Linker Sequence:
None
3' Linker Sequence:
None

Recommended Growth Condition:

Distributed in bacterial strain:
DH5-alpha T1 phage resistant
Antibiotic Selection:
Host Type:  bacterial    Marker:  ampicillin
Bacterial Selection Condition: 100 ug/mL ampicillin
Growth Condition: Growth with the single antibiotic in LB at 37 degrees is recommended.
Comments: Commonly used conditions for ampicillin resistant plasmid clones.
Protein Expression Results:
ProteinExpressed: Protein_Confirmed
ProteinPurified: Protein_Purified
SolubleProtein: Protein_Soluble
Find experimental protocols in MCSG-APC101199
Recommended expression in:
Not Applicable