Lebu_0765 (Leptotrichia buccalis C-1013-b) in pMCSG48

Explanation of Terms

Gene:
Gene Symbol:  Lebu_0765
Gene Name:  endonuclease/exonuclease/phosphatase
Original Clone ID:
APC101229.103
Keyword:
None
Species:
Leptotrichia buccalis C-1013-b
Type:
cDNA
Vector Name:
pMCSG48
Format: 
CLOSED
Source:
Midwest Center for Structural Genomics
Description:
None
Comments:
None
Mutation / Discrepancy:
No / No
Publications:
None
Authors:
Jessica Bearden
PSI:Biology
Argonne National Laboratory
Midwest Center for Structural Genomics
LbCD00520032
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AVAILABLE
No restriction
Special MTA: PSI
Insert sequence: 876nts
Open reading frame: 51 to 926

CCGAGATGGCGCCGTATACACAACGAGAACCTGTACTTCCATCCAATGCCATGAAAAATT
TAAAAATGAAAATTTTAGCATTACTTTTTTGTTTGATTTTTGCATGTACAAAAGAAAATA
TTGAAACAGACTTTTCTCAAATTGAAAAAAATGATAAAACAATAATTATTGCATCATTTA
ATGCTCTTCGGCTTGGAGAAAAGCAGAAGGATTATAAGGCATTTTCAAAAATTCTGGCAA
ATTTTGACTTGATTGGGCTGGAAGAAGTTATGAATGAAAAAGGGGTGAAAAAGGTTCAGG
CACATTTACAAAAATTAACGAAAGAAAAATGGGATTATATAATTTCAGAAGACTCTGTTG
GAAGTGAAAATTACAGGGAGTATTATGCTTTTATTTATAGGCGTGATAAATTTTTAGAAG
CGAAAGGACTTGGATTTTATAAAGAAAAAGATGAAAATGAGTTTATGCGTGAACCTTATG
GAGCTTATTTTAAGGCGGGAAATTTTGACTTTGTCTATATAATCGCCCATTCAGTTTTTG
GAGACAAGGAAAAGCAGAGATTGCTGGAAGCGGCAAATTATGTGAATGTTTATGAATATT
TTTCAAAACTAACTGATGAAGATGATATAATTATCGCAGGGGATTTTAATACAGCAGCAG
ACAATATGGCATTTAAAAATCTTTCTGATAAATACAACGTTTCCTATATTTTAGAGCCAA
ATGAGAATTTAACAACACTTTCAGACAATAAACTGGTAAGCTCATACGACAACTTTTTTA
TCAATTCAAAAAAGACAAAAGAATTTACTGGAAATTCTGGCGTTTATAACTTTATAAAAA
ATAATAATTATGCTATAATAAAAAAATATGTATCAGACCATTTGCCAATTTTTTCAGAAT
ACTCGATAACAGAAGATTTGGATTAACATTGGAAGTGGATAATAA

Protein sequence predicted based on nucleotide sequence

MKNLKMKILALLFCLIFACTKENIETDFSQIEKNDKTIIIASFNALRLGEKQKDYKAFSK
ILANFDLIGLEEVMNEKGVKKVQAHLQKLTKEKWDYIISEDSVGSENYREYYAFIYRRDK
FLEAKGLGFYKEKDENEFMREPYGAYFKAGNFDFVYIIAHSVFGDKEKQRLLEAANYVNV
YEYFSKLTDEDDIIIAGDFNTAADNMAFKNLSDKYNVSYILEPNENLTTLSDNKLVSSYD
NFFINSKKTKEFTGNSGVYNFIKNNNYAIIKKYVSDHLPIFSEYSITEDLD*

Coding Sequence Details

Insert Sequence Verified?:
Y
Verification Method:
Sequence Verification
Mutations:
None
Discrepancy:
None
5' Linker Sequence:
None
3' Linker Sequence:
None

Recommended Growth Condition:

Distributed in bacterial strain:
DH5-alpha T1 phage resistant
Antibiotic Selection:
Host Type:  bacterial    Marker:  ampicillin
Bacterial Selection Condition: 100 ug/mL ampicillin
Growth Condition: Growth with the single antibiotic in LB at 37 degrees is recommended.
Comments: Commonly used conditions for ampicillin resistant plasmid clones.
Protein Expression Results:
ProteinPurified: Not_Tested
ProteinExpressed: Protein_Confirmed
SolubleProtein: Tested_Not_Soluble
Find experimental protocols in MCSG-APC101229
Recommended expression in:
Not Applicable