Plim_0623 (Planctopirus limnophila) in pMCSG48

Explanation of Terms

Gene:
Gene Symbol:  Plim_0623
Gene Name:  beta-lactamase
Original Clone ID:
APC101318.103
Keyword:
None
Species:
Planctopirus limnophila
Type:
cDNA
Vector Name:
pMCSG48
Format: 
CLOSED
Source:
Midwest Center for Structural Genomics
Description:
None
Comments:
None
Mutation / Discrepancy:
No / Yes
Publications:
None
Authors:
Jessica Bearden
PSI:Biology
Argonne National Laboratory
Midwest Center for Structural Genomics
PlCD00520100
Price: 
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AVAILABLE
No restriction
Special MTA: PSI
Insert sequence: 1254nts
Open reading frame: 51 to 1304

GAGATATGGCGCCGTATACACAACGAGAACCTGTACTTCCATCCAATGCCGTGGAATGTG
CACAGACTGAGAAAACAGGTGCAATCTTACGTCTGCACACTTATCATCCCCTCATTATAT
CATCAAGTGATGAATTCTGGCTCGCGCCAGGGCCGTTCGAACGTGAGCGGGAAGATCGTC
AATTCTTGCGAAAGTCACTGGCTATGAATGGCTTGCGAATGGTTGAACAACGGAGTTTTC
GTGTTCTGGCGACAGTGCTGGCGATGGCTGCCTTGTTGTGGCTGCCGAGGAACAGTGTTT
TGGCAGATGAGAAAAATGGTGAACTCGACATTTATTTCATTGATGTCGAGGGGGGAGCTG
CCACGCTGCTGGTGACACCCGCGGGGGAATCGATTCTCCTCGATTCGGGCTATCCTGATT
ACGGCGGCCGGGATTTAAATCGCATTGTGCGCGTGGTGAAAGAGGTCGCTGGTCGAACTC
GAATCGACAAAGCGATCGTCTCTCACTGGCATCTGGATCATTACGGCAATCATGCGGCAC
TCGTCAGCAAAGTTCCCGTTACGGCCTTTTATGACCGGGGAATTCCGGAAGATCTTGCGG
AAGACAAAGGGTTTCCCGAGAGAATCGCCTTGTACCGAGCGGCCTCACAGAACAAGTCGA
CAGCCCTTCCCCCCGGAGACAAACTGCTACTGAAAAATGCTAAATCAGGTCAAGGTGCCC
CGCAACTCACAGCCGAGGTGATCACATCGAGTGGGAAAACCATTCCGAACGAAGGGCCGG
CCAACCCGTTTGTCAGTGAGCACGTCGCGAAACCTGAGGACACTTCCGACAATGCCCGCA
GTTTGAGTCTGCTCTTCAAACTGGGGGATTTCGCCTTTCTGACCTGTGGTGACCTGACGT
GGAACACCGAACATCGGTTGATGTCGCCTAATAACCCGATTGGTCAGGTCGATCTCTTTA
TGGTGACTCACCATGGCCTTGATGTCAGTAACAATCCAGTGCTGGTCAAAGCGATTGAAC
CGGTCGCCACTGTCACCTGCAACGGCCCCACCAAAGGGGGCATGCTACCGGTTCTGAAGA
CACTTGAGACGGTCCCGACTCTCAAGGCTCAGTTTCAGTTGCATCGGAACATCAAGCTGG
GAGATGACGTCCAGCCACCGCCGCACCGGATTGCTAATTCCAGCGATACTGCTGATTGCA
AAGGGCATTACATCAAGGCGTCGGTCTCTCCCGATGGCAAGCAATTTCGTATTCAGGTCG
AAGGCCAGCAGCCGGAAATGTTTAAGACCCTCTTGCCCAAATAACATTGGAAGTGGATAA
TAA

Protein sequence predicted based on nucleotide sequence

VECAQTEKTGAILRLHTYHPLIISSSDEFWLAPGPFEREREDRQFLRKSLAMNGLRMVEQ
RSFRVLATVLAMAALLWLPRNSVLADEKNGELDIYFIDVEGGAATLLVTPAGESILLDSG
YPDYGGRDLNRIVRVVKEVAGRTRIDKAIVSHWHLDHYGNHAALVSKVPVTAFYDRGIPE
DLAEDKGFPERIALYRAASQNKSTALPPGDKLLLKNAKSGQGAPQLTAEVITSSGKTIPN
EGPANPFVSEHVAKPEDTSDNARSLSLLFKLGDFAFLTCGDLTWNTEHRLMSPNNPIGQV
DLFMVTHHGLDVSNNPVLVKAIEPVATVTCNGPTKGGMLPVLKTLETVPTLKAQFQLHRN
IKLGDDVQPPPHRIANSSDTADCKGHYIKASVSPDGKQFRIQVEGQQPEMFKTLLPK*

Coding Sequence Details

Insert Sequence Verified?:
Y
Verification Method:
Sequence Verification
Mutations:
None
Discrepancy:
t619c,S207P
                                                    

5' Linker Sequence:
None
3' Linker Sequence:
None

Recommended Growth Condition:

Distributed in bacterial strain:
DH5-alpha T1 phage resistant
Antibiotic Selection:
Host Type:  bacterial    Marker:  ampicillin
Bacterial Selection Condition: 100 ug/mL ampicillin
Growth Condition: Growth with the single antibiotic in LB at 37 degrees is recommended.
Comments: Commonly used conditions for ampicillin resistant plasmid clones.
Protein Expression Results:
ProteinPurified: Not_Tested
ProteinExpressed: Protein_Confirmed
SolubleProtein: Protein_Soluble
Find experimental protocols in MCSG-APC101318
Recommended expression in:
Not Applicable