EDEM3 (Homo sapiens) in pDONR221 (Gateway donor/master vector)

Explanation of Terms

Gene:
Gene Symbol:  EDEM3
Gene Name:  ER degradation enhancer, mannosidase alpha-like 3
Original Clone ID:
None
Keyword:
None
Species:
Homo sapiens
Type:
cDNA
Vector Name:
pDONR221
Format: 
FUSION
Source:
Glyco-enzyme clone collection
Description:
None
Comments:
for N-term fusion (no start codon)
Mutation / Discrepancy:
No / No
Publications:
None
Authors:
University of Georgia
Center for Personalized Diagnostics
Arizona State University
Kelley Moremen
HsCD00522351
Price: 
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AVAILABLE
No restriction
Special MTA: SPTA
Insert sequence: 2712nts
Open reading frame: 1 to 2712

GCCGAGCCCATGAGTAGGGAGGAGAAACAGAAGCTTGGGAATCAAGTACTGGAAATGTTT
GATCATGCTTATGGTAACTATATGGAACATGCTTACCCTGCTGATGAACTCATGCCTTTA
ACCTGTAGAGGTCGAGTTAGAGGCCAAGAGCCAAGTCGCGGTGACGTTGATGATGCCTTG
GGAAAATTTTCTCTGACACTGATTGATTCTTTGGACACTCTTGTGGTTTTAAATAAAACT
AAAGAATTTGAAGATGCAGTGAGAAAAGTTTTAAGAGATGTTAATTTAGATAACGATGTA
GTCGTATCAGTCTTTGAAACAAACATCAGAGTTCTTGGGGGTCTTTTGGGTGGGCACTCC
CTGGCAATCATGCTGAAAGAAAAAGGTGAATATATGCAGTGGTACAATGATGAACTTCTC
CAAATGGCAAAGCAGTTAGGTTACAAACTTTTACCGGCTTTCAACACTACCAGTGGCCTT
CCTTATCCAAGAATTAATTTAAAGTTTGGCATCAGAAAACCAGAAGCTCGGACAGGAACT
GAGACAGATACCTGTACAGCTTGTGCAGGTACCTTGATCCTTGAATTTGCTGCTTTAAGT
CGATTCACAGGAGCAACAATATTTGAGGAATATGCCAGAAAAGCTCTTGATTTTCTCTGG
GAAAAAAGACAGCGAAGTAGTAATTTAGTGGGCGTGACTATAAATATTCATACTGGAGAT
TGGGTACGAAAAGATAGTGGAGTTGGAGCAGGGATTGATTCATATTATGAATATCTGTTG
AAAGCCTATGTCTTGCTTGGAGATGACAGTTTTCTGGAAAGATTTAACACACACTATGAT
GCCATAATGAGGTATATTAGCCAGCCACCTCTTCTACTTGATGTGCATATCCACAAACCA
ATGCTGAATGCTCGGACTTGGATGGATGCTTTGCTTGCCTTCTTCCCAGGCTTGCAGGTG
TTAAAGGGGGATATTAGACCTGCTATTGAAACTCATGAAATGTTATATCAGGTGATTAAA
AAACACAATTTTCTACCAGAGGCATTTACCACAGATTTCAGAGTACACTGGGCTCAACAT
CCTTTAAGGCCAGAATTTGCAGAAAGTACCTACTTCTTATATAAAGCTACAGGAGATCCT
TACTACCTTGAAGTAGGGAAGACACTTATTGAAAATTTAAATAAATATGCTAGAGTGCCT
TGCGGATTTGCTGCCATGAAGGATGTTCGTACTGGAAGTCATGAGGACAGAATGGATTCT
TTCTTCTTGGCTGAAATGTTTAAATATCTTTACCTGTTATTTGCTGATAAAGAAGACATT
ATTTTTGACATAGAAGATTACATCTTTACAACAGAAGCTCATCTGTTACCTCTTTGGCTC
TCTACTACAAATCAAAGCATCTCTAAAAAGAACACAACCTCGGAATATACAGAACTGGAT
GACAGTAACTTCGATTGGACTTGTCCAAATACTCAGATCCTCTTTCCTAATGACCCATTG
TATGCTCAAAGTATTCGTGAGCCCTTGAAAAATGTGGTGGATAAGAGCTGTCCTAGAGGC
ATCATCAGAGTAGAGGAGAGTTTCAGGAGTGGAGCTAAACCCCCTCTGAGAGCCAGAGAT
TTCATGGCCACTAACCCTGAGCATTTAGAAATCCTGAAGAAGATGGGGGTGAGTTTGATT
CACCTCAAAGATGGGAGAGTCCAGTTGGTCCAACATGCAATCCAAGCTGCTAGTTCAATC
GACGCTGAAGATGGGTTGAGGTTCATGCAGGAGATGATTGAATTGTCAAGTCAGCAACAA
AAAGAACAGCAGCTGCCTCCACGAGCTGTACAAATTGTTTCCCACCCATTTTTTGGCAGG
GTAGTATTGACTGCTGGACCAGCTCAGTTTGGGCTGGATCTGTCTAAACATAAAGAGACA
AGAGGATTTGTTGCAAGCAGTAAACCATCCAATGGTTGTTCAGAGCTTACTAACCCAGAG
GCAGTGATGGGAAAAATCGCACTGATACAAAGAGGACAGTGCATGTTTGCAGAAAAGGCA
CGCAACATCCAGAATGCTGGAGCCATTGGTGGCATTGTTATTGATGACAATGAGGGGAGC
AGCAGTGATACTGCCCCTCTGTTCCAGATGGCAGGTGATGGAAAGGATACAGATGACATC
AAGATCCCCATGCTGTTCTTATTCAGCAAAGAAGGAAGTATCATACTGGATGCCATCCGG
GAATATGAGGAGGTAGAAGTGCTCCTCTCTGATAAAGCAAAAGATCGAGCAGCAATATTA
AAAGGTAAAATGATTCCAAGCTACATTATTAATAGTAATCCTGAAATGGAAAATGAAGAA
CAACCATCCTCTGAAAATGATTCTCAGAATCAGAGTGGTGAACAGATTTCATCAAGTTCT
CAGGAGGTTGATTTGGTTGATCAAGAGTCTTCTGAGGAAAATTCTCTAAATTCTCACCCA
GAATCATTATCTCTAGCAGATATGGACAATGCTGCAAGCATTTCCCCTTCTGAACAGACT
TCTAATCCCACAGAAAACCATGAGACTACAAATCTTAATGGTGAATGTACAGATTTAGAT
AACCAGCTTCAAGAACAATCAGAAACTGAGGAAGATTCCAATCCTAATGTTAGCTGGGGT
AAAAAGGTCCAGCCTATAGACTCCATATTAGCAGACTGGAATGAAGATATAGAAGCATTT
GAAATGATGGAG

Protein sequence predicted based on nucleotide sequence

AEPMSREEKQKLGNQVLEMFDHAYGNYMEHAYPADELMPLTCRGRVRGQEPSRGDVDDAL
GKFSLTLIDSLDTLVVLNKTKEFEDAVRKVLRDVNLDNDVVVSVFETNIRVLGGLLGGHS
LAIMLKEKGEYMQWYNDELLQMAKQLGYKLLPAFNTTSGLPYPRINLKFGIRKPEARTGT
ETDTCTACAGTLILEFAALSRFTGATIFEEYARKALDFLWEKRQRSSNLVGVTINIHTGD
WVRKDSGVGAGIDSYYEYLLKAYVLLGDDSFLERFNTHYDAIMRYISQPPLLLDVHIHKP
MLNARTWMDALLAFFPGLQVLKGDIRPAIETHEMLYQVIKKHNFLPEAFTTDFRVHWAQH
PLRPEFAESTYFLYKATGDPYYLEVGKTLIENLNKYARVPCGFAAMKDVRTGSHEDRMDS
FFLAEMFKYLYLLFADKEDIIFDIEDYIFTTEAHLLPLWLSTTNQSISKKNTTSEYTELD
DSNFDWTCPNTQILFPNDPLYAQSIREPLKNVVDKSCPRGIIRVEESFRSGAKPPLRARD
FMATNPEHLEILKKMGVSLIHLKDGRVQLVQHAIQAASSIDAEDGLRFMQEMIELSSQQQ
KEQQLPPRAVQIVSHPFFGRVVLTAGPAQFGLDLSKHKETRGFVASSKPSNGCSELTNPE
AVMGKIALIQRGQCMFAEKARNIQNAGAIGGIVIDDNEGSSSDTAPLFQMAGDGKDTDDI
KIPMLFLFSKEGSIILDAIREYEEVEVLLSDKAKDRAAILKGKMIPSYIINSNPEMENEE
QPSSENDSQNQSGEQISSSSQEVDLVDQESSEENSLNSHPESLSLADMDNAASISPSEQT
SNPTENHETTNLNGECTDLDNQLQEQSETEEDSNPNVSWGKKVQPIDSILADWNEDIEAF
EMME

Coding Sequence Details

Insert Sequence Verified?:
Y
Verification Method:
Sequence Verification
Mutations:
None
Discrepancy:
None
5' Linker Sequence:
None
3' Linker Sequence:
None

Recommended Growth Condition:

Distributed in bacterial strain:
DH5-alpha T1 phage resistant
Antibiotic Selection:
Host Type:  bacterial    Marker:  kanamycin
Bacterial Selection Condition: 50ug/mL kanamycin
Growth Condition: Growth with the single antibiotic in LB at 37 degrees is recommended.
Comments: Conditions for Gateway-type vectors in recombined (with insert) form and other kanamycin resistant vectors.
Protein Expression Results:
None
Recommended expression in:
Not Applicable