MGAM (Homo sapiens) in pDONR221 (Gateway donor/master vector)

Explanation of Terms

Gene:
Gene Symbol:  MGAM
Gene Name:  maltase-glucoamylase (alpha-glucosidase)
Original Clone ID:
None
Keyword:
partial cds (N-terminal deletion)
Species:
Homo sapiens
Type:
cDNA
Vector Name:
pDONR221
Format: 
CLOSED
Source:
Glyco-enzyme clone collection
Description:
None
Comments:
for N-term fusion (no start codon)
Mutation / Discrepancy:
No / No
Publications:
None
Authors:
University of Georgia
Center for Personalized Diagnostics
Arizona State University
Kelley Moremen
HsCD00522644
Price: 
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AVAILABLE
No restriction
Special MTA: SPTA
Insert sequence: 5003nts
Open reading frame: 1 to 5003

AAAGAGTCACTGAAATCAACAGCCCCAGATCCTGGGACAACTGGTACCCCAGATCCTGGG
ACAACTGGTACCCCAGATCCTGGAACAACTGGTACCACACATGCTAGGACAACGGGTCCC
CCAGATCCTGGAACAACTGGTACCACTCCTGTTTCTGCTGAATGTCCAGTGGTAAATGAA
TTGGAACGAATTAATTGCATCCCTGACCAGCCGCCAACAAAGGCCACATGTGACCAACGT
GGCTGTTGCTGGAATCCCCAGGGAGCTGTAAGTGTTCCCTGGTGCTACTATTCCAAGAAT
CATAGCTACCATGTAGAGGGCAACCTTGTCAACACAAATGCAGGATTCACAGCCCGGTTG
AAAAATCTGCCTTCTTCACCAGTGTTTGGAAGCAATGTTGACAATGTTCTTCTCACAGCA
GAATATCAGACATCTAATCGTTTCCACTTTAAGTTGACTGACCAAACCAATAACAGGTTT
GAAGTGCCCCACGAACACGTGCAGTCCTTCAGTGGAAATGCTGCTGCTTCTTTGACCTAC
CAAGTTGAAATCTCCAGACAGCCATTTAGCATCAAAGTGACCAGAAGAAGCAACAATCGT
GTTTTGTTTGACTCGAGCATTGGGCCCCTACTGTTTGCTGACCAGTTCTTGCAGCTCTCC
ACTCGACTGCCTAGCACTAACGTGTATGGCCTGGGAGAGCATGTGCACCAGCAGTATCGG
CATGATATGAATTGGAAGACCTGGCCCATATTTAACAGAGACACAACTCCCAATGGAAAC
GGAACTAATTTGTATGGTGCGCAGACATTCTTCTTGTGCCTTGAAGATGCTAGTGGATTG
TCCTTTGGGGTGTTTCTGATGAACAGCAATGCCATGGAGGTTGTCCTTCAGCCTGCGCCA
GCCATCACTTACCGCACCATTGGGGGCATTCTCGACTTCTATGTGTTCTTGGGAAACACT
CCAGAGCAAGTTGTTCAAGAATATCTAGAGCTCATTGGGCGGCCAGCCCTTCCCTCCTAC
TGGGCGCTTGGATTTCACCTCAGTCGTTACGAATATGGAACCTTAGACAACATGAGGGAA
GTCGTGGAGAGAAATCGCGCAGCACAGCTCCCTTATGATGTTCAGCATGCTGATATTGAT
TATATGGATGAGAGAAGGGACTTCACTTATGATTCAGTGGATTTTAAAGGCTTCCCTGAA
TTTGTCAACGAGTTACACAATAATGGACAGAAGCTTGTCATCATTGTGGATCCAGCCATC
TCCAACAACTCTTCCTCAAGTAAACCCTATGGCCCATATGACAGGGGTTCAGATATGAAG
ATATGGGTGAATAGTTCAGATGGAGTGACTCCACTCATTGGGGAGGTCTGGCCTGGACAA
ACTGTGTTTCCTGATTATACCAATCCCAACTGTGCTGTTTGGTGGACAAAGGAATTTGAG
CTTTTTCACAATCAAGTAGAGTTTGATGGAATCTGGATTGATATGAATGAAGTCTCCAAC
TTTGTTGATGGTTCGGTCTCAGGATGTTCCACAAACAACCTAAATAATCCCCCATTCACT
CCCAGAATCCTGGATGGGTACCTGTTCTGCAAGACTCTCTGTATGGATGCAGTGCAGCAC
TGGGGCAAGCAGTATGACATTCACAATCTGTATGGCTACTCCATGGCGGTCGCCACAGCA
GAAGCTGCCAAGACTGTGTTCCCTAATAAGAGAAGCTTCATTCTGACCCGTTCTACCTTT
GCGGGCTCTGGCAAGTTTGCAGCACATTGGTTAGGAGACAACACTGCCACCTGGGATGAC
CTGAGATGGTCCATCCCTGGCGTGCTTGAGTTCAACCTTTTTGGCATCCCAATGGTGGGT
CCTGACATATGTGGCTTTGCTTTGGACACCCCTGAGGAGCTCTGTAGGCGGTGGATGCAG
TTGGGTGCATTTTATCCGTTTTCTAGAAATCACAATGGCCAAGGCTACAAGGACCAGGAT
CCTGCCTCCTTTGGAGCTGACTCCCTGCTGTTGAATTCCTCCAGGCACTACCTTAACATC
CGCTATACTCTATTGCCCTACCTATACACCCTCTTCTTCCGTGCTCACAGCCGAGGGGAC
ACGGTGGCCAGGCCCCTTTTGCATGAGTTCTACGAGGACAACAGCACTTGGGATGTGCAC
CAACAGTTCTTATGGGGGCCCGGCCTCCTCATCACTCCAGTTCTGGATGAAGGTGCAGAG
AAAGTGATGGCATATGTGCCTGATGCTGTCTGGTATGACTACGAGACTGGGAGCCAAGTG
AGATGGAGGAAGCAAAAAGTCGAGATGGAACTTCCTGGAGACAAAATTGGACTTCACCTT
CGAGGAGGCTACATCTTCCCCACACAGCAGCCAAATACAACCACTCTGGCCAGTCGAAAG
AACCCTCTTGGTCTTATCATTGCCCTAGATGAGAACAAAGAAGCAAAAGGAGAACCTTTC
TGGGATGATGGGGAAACGAAGGATACTGTGGCCAATAAAGTGTATCTTTTATGTGAGTTT
TCTGTCACTCAAAACCGCTTGGAGGTGAATATTTCACAATCAACCTACAAGGACCCCAAT
AATTTAGCATTTAATGAGATTAAAATTCTTGGGACGGAGGAACCTAGCAATGTTACAGTG
AAACACAATGGTGTCCCAAGTCAGACTTCTCCTACAGTCACTTATGATTCTAACCTGAAG
GTTGCCATTATCACAGATATTGATCTTCTCCTGGGAGAAGCATACACAGTGGAATGGAGC
ATAAAGATAAGGGATGAAGAAAAAATAGACTGTTACCCTGATGAGAATGGTGCTTCTGCC
GAAAACTGCACTGCCCGTGGCTGTATCTGGGAGGCATCCAATTCTTCTGGAGTCCCTTTT
TGCTATTTTGTCAACGACCTATACTCTGTCAGTGATGTTCAGTATAATTCCCATGGGGCC
ACAGCTGACATCTCCTTAAAGTCTTCCGTTTATGCCAATGCCTTCCCCTCCACACCCGTG
AACCCCCTTCGCCTGGATGTCACTTACCATAAGAATGAAATGCTGCAGTTCAAGATTTAT
GATCCCAACAAGAATCGGTATGAAGTTCCAGTCCCTCTGAACATACCCAGCATGCCATCC
AGCACCCCTGAGGGTCAACTCTATGATGTGCTCATTAAGAAGAATCCATTTGGGATTGAA
ATTCGCCGGAAGAGTACAGGCACTATAATCTGGGACTCTCAGCTCCTTGGCTTTACCTTC
AGTGACATGTTTATCCGCATCTCCACCCGCCTTCCCTCCAAGTACCTCTATGGCTTTGGG
GAAACTGAGCACAGGTCCTATAGGAGAGACTTGGAGTGGCACACTTGGGGGATGTTCTCC
CGAGACCAGCCCCCAGGGTACAAGAAGAATTCCTATGGTGTCCACCCCTACTACATGGGG
CTGGAGGAGGACGGCAGTGCCCATGGAGTGCTCCTGCTGAACAGCAATGCCATGGATGTG
ACGTTCCAGCCCCTGCCTGCCTTGACATACCGCACCACAGGGGGAGTTCTGGACTTTTAT
GTGTTCTTGGGGCCGACTCCAGAGCTTGTCACCCAGCAGTACACTGAGTTGATTGGCCGG
CCTGTGATGGTACCTTACTGGTCTTTGGGGTTCCAGCTGTGTCGCTATGGCTACCAGAAT
GACTCTGAGATCGCCAGCTTGTATGATGAGATGGTGGCTGCCCAGATCCCTTATGATGTG
CAGTACTCAGACATCGACTACATGGAGCGGCAGCTGGACTTCACCCTCAGCCCCAAGTTT
GCTGGGTTTCCAGCTCTGATCAATCGCATGAAGGCTGATGGGATGCGGGTCATCCTCATT
CTGGATCCAGCCATTTCTGGCAACGAGACACAGCCTTATCCTGCCTTCACTCGGGGCGTG
GAGGATGACGTCTTCATCAAATACCCAAATGATGGAGACATTGTCTGGGGAAAGGTCTGG
CCTGATTTTCCTGATGTTGTTGTGAATGGGTCTCTAGACTGGGACAGCCAAGTGGAGCTA
TATCGAGCTTATGTGGCCTTCCCAGACTTTTTCCGTAATTCAACTGCCAAGTGGTGGAAG
AGGGAAATAGAAGAACTATACAACAATCCACAGAATCCAGAGAGGAGCTTGAAGTTTGAT
GGCATGTGGATTGATATGAATGAACCATCAAGCTTCGTGAATGGGGCAGTTTCTCCAGGC
TGCAGGGACGCCTCTCTGAACCACCCTCCCTACATGCCACATTTGGAGTCCAGGGACAGG
GGCCTGAGCAGCAAGACCCTTTGTATGGAGAGTCAGCAGATCCTCCCAGACGGCTCCCTG
GTGCAGCACTACAACGTGCACAACCTGTATGGGTGGTCCCAGACCAGACCCACATACGAA
GCCGTGCAGGAGGTGACGGGACAGCGAGGGGTCGTCATCACCCGCTCCACATTTCCCTCT
TCTGGCCGCTGGGCAGGACATTGGCTGGGAGACAACACGGCCGCATGGGATCAGCTGAAG
AAGTCTATCATTGGCATGATGGAGTTCAGCCTCTTCGGCATATCCTATACGGGAGCAGAT
ATCTGTGGGTTCTTTCAAGATGCTGAATATGAGATGTGTGTTCGCTGGATGCAGCTGGGG
GCCTTTTACCCCTTCTCAAGAAACCACAACACCATTGGGACCAGGAGACAAGACCCTGTG
TCCTGGGATGTTGCTTTTGTGAATATTTCCAGAACTGTCCTGCAGACCAGATACACCCTG
TTGCCATATCTGTATACCTTGATGCATAAGGCCCACACGGAGGGCGTCACTGTTGTGCGG
CCTCTGCTCCATGAATTTGTGTCAGACCAGGTGACATGGGACATAGACAGTCAGTTCCTG
CTGGGCCCAGCCTTCCTGGTCAGCCCTGTCCTGGAGCGTAATGCCAGAAATGTCACTGCA
TATTTCCCTAGAGCCCGCTGGTATGATTACTACACGGGTGTGGATATTAATGCAAGAGGA
GAGTGGAAGACCTTGCCAGCTAG

Protein sequence predicted based on nucleotide sequence

KESLKSTAPDPGTTGTPDPGTTGTPDPGTTGTTHARTTGPPDPGTTGTTPVSAECPVVNE
LERINCIPDQPPTKATCDQRGCCWNPQGAVSVPWCYYSKNHSYHVEGNLVNTNAGFTARL
KNLPSSPVFGSNVDNVLLTAEYQTSNRFHFKLTDQTNNRFEVPHEHVQSFSGNAAASLTY
QVEISRQPFSIKVTRRSNNRVLFDSSIGPLLFADQFLQLSTRLPSTNVYGLGEHVHQQYR
HDMNWKTWPIFNRDTTPNGNGTNLYGAQTFFLCLEDASGLSFGVFLMNSNAMEVVLQPAP
AITYRTIGGILDFYVFLGNTPEQVVQEYLELIGRPALPSYWALGFHLSRYEYGTLDNMRE
VVERNRAAQLPYDVQHADIDYMDERRDFTYDSVDFKGFPEFVNELHNNGQKLVIIVDPAI
SNNSSSSKPYGPYDRGSDMKIWVNSSDGVTPLIGEVWPGQTVFPDYTNPNCAVWWTKEFE
LFHNQVEFDGIWIDMNEVSNFVDGSVSGCSTNNLNNPPFTPRILDGYLFCKTLCMDAVQH
WGKQYDIHNLYGYSMAVATAEAAKTVFPNKRSFILTRSTFAGSGKFAAHWLGDNTATWDD
LRWSIPGVLEFNLFGIPMVGPDICGFALDTPEELCRRWMQLGAFYPFSRNHNGQGYKDQD
PASFGADSLLLNSSRHYLNIRYTLLPYLYTLFFRAHSRGDTVARPLLHEFYEDNSTWDVH
QQFLWGPGLLITPVLDEGAEKVMAYVPDAVWYDYETGSQVRWRKQKVEMELPGDKIGLHL
RGGYIFPTQQPNTTTLASRKNPLGLIIALDENKEAKGEPFWDDGETKDTVANKVYLLCEF
SVTQNRLEVNISQSTYKDPNNLAFNEIKILGTEEPSNVTVKHNGVPSQTSPTVTYDSNLK
VAIITDIDLLLGEAYTVEWSIKIRDEEKIDCYPDENGASAENCTARGCIWEASNSSGVPF
CYFVNDLYSVSDVQYNSHGATADISLKSSVYANAFPSTPVNPLRLDVTYHKNEMLQFKIY
DPNKNRYEVPVPLNIPSMPSSTPEGQLYDVLIKKNPFGIEIRRKSTGTIIWDSQLLGFTF
SDMFIRISTRLPSKYLYGFGETEHRSYRRDLEWHTWGMFSRDQPPGYKKNSYGVHPYYMG
LEEDGSAHGVLLLNSNAMDVTFQPLPALTYRTTGGVLDFYVFLGPTPELVTQQYTELIGR
PVMVPYWSLGFQLCRYGYQNDSEIASLYDEMVAAQIPYDVQYSDIDYMERQLDFTLSPKF
AGFPALINRMKADGMRVILILDPAISGNETQPYPAFTRGVEDDVFIKYPNDGDIVWGKVW
PDFPDVVVNGSLDWDSQVELYRAYVAFPDFFRNSTAKWWKREIEELYNNPQNPERSLKFD
GMWIDMNEPSSFVNGAVSPGCRDASLNHPPYMPHLESRDRGLSSKTLCMESQQILPDGSL
VQHYNVHNLYGWSQTRPTYEAVQEVTGQRGVVITRSTFPSSGRWAGHWLGDNTAAWDQLK
KSIIGMMEFSLFGISYTGADICGFFQDAEYEMCVRWMQLGAFYPFSRNHNTIGTRRQDPV
SWDVAFVNISRTVLQTRYTLLPYLYTLMHKAHTEGVTVVRPLLHEFVSDQVTWDIDSQFL
LGPAFLVSPVLERNARNVTAYFPRARWYDYYTGVDINARGEWKTLPA

Coding Sequence Details

Insert Sequence Verified?:
Y
Verification Method:
Sequence Verification
Mutations:
None
Discrepancy:
None
5' Linker Sequence:
None
3' Linker Sequence:
None

Recommended Growth Condition:

Distributed in bacterial strain:
DH5-alpha T1 phage resistant
Antibiotic Selection:
Host Type:  bacterial    Marker:  kanamycin
Bacterial Selection Condition: 50ug/mL kanamycin
Growth Condition: Growth with the single antibiotic in LB at 37 degrees is recommended.
Comments: Conditions for Gateway-type vectors in recombined (with insert) form and other kanamycin resistant vectors.
Protein Expression Results:
None
Recommended expression in:
Not Applicable