Gene:
Gene Symbol:
Gene Name:
Original Clone ID:
IMPT-1426
Keyword:
None
Species:
Homo sapiens
Type:
cDNA
Vector Name:
pFASTBAC1
Format:
CLOSED
Source:
GPCR Network
Description:
None
Comments:
None
Mutation / Discrepancy:
No / No
Publications:
None
Authors:
The Scripps Research Institute
PSI:Biology
GPCR Network
PSI:Biology
GPCR Network
HsCD00525876
Price:
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AVAILABLE
No restriction
Special MTA: PSI
Special MTA: PSI
Insert sequence: 1362nts
Open reading frame: 1 to 1362
ATGAAGACGATCATCGCCCTGAGCTACATCTTCTGCCTGGTGTTCGCCGGCGCGCCGGAT
TATCAAGTGTCAAGTCCAATCTATGACATCAATTATTATACATCGGAGCCCTGCCAAAAA
ATCAATGTGAAGCAAATCGCAGCCCGCCTCCTGCCTCCGCTCTACTCACTGGTGTTCATC
TTTGGTTTTGTGGGCAACATGCTGGTCATCCTCATCCTGATAAACTGCAAAAGGCTGAAG
AGCATGACTGACATCTACCTGCTCAACCTGGCCATCTCTGACCTGTTTTTCCTTCTTACT
GTCCCCTTCTGGGCTCACTATGCTGCCGCCCAGTGGGACTTTGGAAATACAATGTGTCAA
CTCTTGACAGGGCTCTATTTTATAGGCTTCTTCTCTGGAATCTTCTTCATCATCCTCCTG
ACAATCGATAGGTACCTGGCTGTCGTCCATGCTGTGTTTGCTTTAAAAGCCAGGACGGTC
ACCTTTGGGGTGGTGACAAGTGTGATCACTTGGGTGGTGGCTGTGTTTGCGTCTCTCCCA
GGAATCATCTTTACCAGATCTCAAAAAGAAGGTCTTCATTACACCTGCAGCTCTCATTTT
CCATACAGTCAGTATCAATTCTGGAAGAATTTCCAGACATTAAAGATAGTCATCTTGGGG
CTGGTCCTGCCGCTGCTTGTCATGGTCATCTGCTACTCGGGAATCCTAAAAACTCTGCTT
CGGTGTATGAAGAAGTATACCTGTACCGTCTGCGGATACATTTACAACCCCGAGGACGGC
GACCCTGATAACGGCGTCAACCCCGGAACTGACTTCAAGGATATTCCTGACGATTGGGTC
TGTCCCCTCTGTGGCGTCGGCAAGGACCAATTTGAAGAGGTGGAGGAACGAAATGAGAAG
AAGAGGCACAGGGCTGTGAGGCTTATCTTCACCATCATGATTGTTTATTTTCTCTTCTGG
GCTCCCTACAACATTGTCCTTCTCCTGAACACCTTCCAGGAATTCTTTGGCCTGAATAAT
TGCAGTAGCTCTAACAGGTTGGACCAAGCTATGCAGGTGACAGAGACTCTTGGGATGACG
CACTGCTGCATCAACCCCATCATCTATGCCTTTGTCGGGGAGAAGTTCAGAAACTACCTC
TTAGTCTTCTTCCAAAAGCACATTGCCAAACGCTTCTGCAAATGCTGTTCTATTTTCCAG
CAAGAGGCTCCCGAGCGAGCAAGCTCAGTTTACACCCGATCCACTGGGGAGCAGGAAATA
TCTGTGGGCTTGGGCCGGCCTCTGGAAGTTCTGTTCCAGGGGCCCCATCATCATCATCAT
CATCATCATCATCATGACTACAAAGACGATGACGACAAGTAG
Protein sequence predicted based on nucleotide sequence
MKTIIALSYIFCLVFAGAPDYQVSSPIYDINYYTSEPCQKINVKQIAARLLPPLYSLVFI FGFVGNMLVILILINCKRLKSMTDIYLLNLAISDLFFLLTVPFWAHYAAAQWDFGNTMCQ LLTGLYFIGFFSGIFFIILLTIDRYLAVVHAVFALKARTVTFGVVTSVITWVVAVFASLP GIIFTRSQKEGLHYTCSSHFPYSQYQFWKNFQTLKIVILGLVLPLLVMVICYSGILKTLL RCMKKYTCTVCGYIYNPEDGDPDNGVNPGTDFKDIPDDWVCPLCGVGKDQFEEVEERNEK KRHRAVRLIFTIMIVYFLFWAPYNIVLLLNTFQEFFGLNNCSSSNRLDQAMQVTETLGMT HCCINPIIYAFVGEKFRNYLLVFFQKHIAKRFCKCCSIFQQEAPERASSVYTRSTGEQEI SVGLGRPLEVLFQGPHHHHHHHHHHDYKDDDDK*
Coding Sequence Details
Insert Sequence Verified?:
Y
Verification Method:
Sequence Verification
Mutations:
None
Discrepancy:
None
5' Linker Sequence:
None
3' Linker Sequence:
None
Recommended Growth Condition:
Distributed in bacterial strain:
DH5-alpha T1 phage resistant
Antibiotic Selection:
Host Type:
bacterial
Marker:
ampicillin
Bacterial Selection Condition:
100 ug/mL ampicillin
Growth Condition:
Growth with the single antibiotic in LB at 37 degrees is recommended.
Comments:
Commonly used conditions for ampicillin resistant plasmid clones.
Protein Expression Results:
SolubleProtein: Not_Applicable
ProteinExpressed: Protein_Confirmed
ProteinPurified: Protein_Purified
Find experimental protocols in GPCR-28
Recommended expression in:
Not Applicable
Vector Name:
pFASTBAC1
Synonyms:
pFB-1, pFB1
Sequencing Primer:
Forward:
pFB Forward
Reverse:
pFB Reverse
Description:
Transfer vector (to E. coli from expression bacmid) with polyhedrin promoter, adds SV40 polyA site; amp resistance (gentamycin for selection in insect cells); restriction enzyme cloning.
Comments:
requires transformation into E. coli DH10Bac for bacmid production
Size (bp):
4775
Parent Vector:
None
Properties:
baculovirus/insect cell expression, multiple cloning site
Author Name:
Midwest Center for Structural Genomics
Publications:
None
Vector Map:
Vector Sequence:
Sequence:
Vector Features:
Type
Name
Description
Start
End
MCS
MCS
MCS (includes BamHI, EcoRI, NotI, XhoI)
4037
4142
bacterial origin
pUC ori
pUC-type origin of replication
1594
2267
poly-A signal
polyA
SV40 polyA signal sequence
4205
4396
promoter
PH
polyhedrin promoter for insect cell expression
3904
4032
selectable marker
GenR
gentamycin resistance gene
3000
3533
selectable marker
AmpR
ampicillin resistance gene
787
1446
ssDNA origin
f1
f1 origin of replication
2
19
transposon fragment
Tn7L
Tn7L transposon site
4429
4594
transposon fragment
Tn7R
Tn7R transposon site
2709
2933
