PFA3 (S. cerevisiae )
In pSGP46 (ZZ and His-tagged yeast expression vector)

Explanation of Terms

Gene:
Gene Symbol:  PFA3
Gene Name:  Pfa3p
Original Clone ID:
YNL326C_pSGP46
Keyword:
None
Species:
Saccharomyces cerevisiae
Type:
cDNA
Vector Name:
pSGP46
Format: 
FUSION
Source:
Membrane Protein Structural Biology Consortium
Description:
None
Comments:
None
Mutation / Discrepancy:
No / No
Publications:
None
Authors:
University of Rochester
PSI:Biology
Membrane Protein Structural Biology Consortium
ScCD00525883
Price: 
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AVAILABLE
No restriction
Special MTA: PSI
Insert sequence: 1008nts
Open reading frame: 29 to 1036

AGATCGACACTTCTTAACAATATACAAAATGAATGACAGGCTTTCGTTGACAAGTCTGTT
TCCCAGGTGTTTGACCACATGTTTGTATATTTGGACTGCATACATAACATTGACAAGGAT
TCATCAAATACCTAGGTGGTTTCTCGCGCTAACAATAGTTCCGACATTGGCAGTGGCTCT
ATACACTTATTATAAGGTTATTGCTAGAGGTCCCGGTTCTCCTCTAGATTTCCCTGATTT
GTTAGTCCACGATTTAAAGGCAGCGGAAAATGGACTTGAACTTCCTCCGGAGTATATGTC
AAAAAGGTGTTTGACCTTAAAACATGATGGAAGATTTCGGGTGTGCCAAGTATGCCATGT
TTGGAAGCCCGATCGCTGTCATCATTGTTCATCATGCGATGTTTGCATATTGAAAATGGA
TCATCATTGCCCTTGGTTCGCTGAATGCACTGGTTTTAGGAATCAAAAATTTTTTATTCA
ATTCTTAATGTACACAACATTATACGCGTTCTTAGTCCTAATTTATACTTGCTATGAGCT
TGGGACGTGGTTTAACTCCGGTAGTTTCAACCGTGAATTGATAGATTTTCATCTATTAGG
GGTGGCCCTATTGGCGGTTGCTGTTTTCATTTCTGTTTTAGCATTTACCTGTTTCAGCAT
TTATCAGGTTTGTAAAAACCAAACTACTATCGAGGTACACGGAATGAGAAGGTATCGAAG
GGATCTTGAAATCCTCAATGACAGCTATGGAACAAATGAGCATTTAGAGAATATATTTGA
CTTGGGTTCTTCAATGGCCAACTGGCAAGATATAATGGGTACATCTTGGTTAGAGTGGAT
ACTTCCTATTGAGACCTTCAAGTACAAAAAGTCTAAACACACAAAGGATGAAAAAGGTTT
ATATTTTAATGTAAGACCTCAAGTACAAGACCGACTGCTAAGTAGTAGATGTTTAGAAGA
TCAATTACTGAGAAGAGTAACACCAAGACCCTCATTAGAAGCCGATAGGGCGTCTGTAGA
AATTATCGANTGCAATAGTGGTTTGGAGGTTTTGTTTCAAGGTCCAAACGGTGCTCCATC
GGCCGTG

Protein sequence predicted based on nucleotide sequence

MNDRLSLTSLFPRCLTTCLYIWTAYITLTRIHQIPRWFLALTIVPTLAVALYTYYKVIAR
GPGSPLDFPDLLVHDLKAAENGLELPPEYMSKRCLTLKHDGRFRVCQVCHVWKPDRCHHC
SSCDVCILKMDHHCPWFAECTGFRNQKFFIQFLMYTTLYAFLVLIYTCYELGTWFNSGSF
NRELIDFHLLGVALLAVAVFISVLAFTCFSIYQVCKNQTTIEVHGMRRYRRDLEILNDSY
GTNEHLENIFDLGSSMANWQDIMGTSWLEWILPIETFKYKKSKHTKDEKGLYFNVRPQVQ
DRLLSSRCLEDQLLRRVTPRPSLEADRASVEIICN

Coding Sequence Details

Insert Sequence Verified?:
Y
Verification Method:
Sequence Verification
Mutations:
None
Discrepancy:
None
5' Linker Sequence:
None
3' Linker Sequence:
None

Recommended Growth Condition:

Distributed in bacterial strain:
DH5-alpha T1 phage resistant
Antibiotic Selection:
Host Type:  yeast    Marker:  ampicillin
Bacterial Selection Condition: 100 ug/mL ampicillin
Growth Condition: Growth with the single antibiotic in LB at 37 degrees is recommended.
Comments: Commonly used conditions for ampicillin resistant plasmid clones.
Protein Expression Results:
SolubleProtein: Not_Applicable
ProteinExpressed: Protein_Confirmed
ProteinPurified: Tested_Not_Purified
Find experimental protocols in MPSBC-Pfa3p
Recommended expression in:
Not Applicable