kpsF (R. etli CFN 42 )
In pNIC28-Bsa4 (His-tagged bacterial expression vector)

Explanation of Terms

Gene:
Gene Symbol:  kpsF
Gene Name:  polysialic acid capsule expression protein
Original Clone ID:
014442
Keyword:
None
Species:
Rhizobium etli CFN 42
Type:
cDNA
Vector Name:
pNIC28-Bsa4
Format: 
CLOSED
Source:
New York Structural Genomics Research Consortium
Description:
None
Comments:
None
Mutation / Discrepancy:
No / No
Publications:
None
Authors:
Albert Einstein College of Medicine
Steven Almo
PSI:Biology
New York Structural Genomics Research Consortium
ReCD00526122
Price: 
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AVAILABLE
No restriction
Special MTA: PSI
Insert sequence: 993nts
Open reading frame: 11 to 1003

CCAATCCATGAACAGAAGAGCGATCAAACTCGTTGAAAACAGCGTGCTCGAATCGGCAAA
ACGCACGATAGAGACCGAGAAACACGGTCTGGAAGCGCTCGAACGGGCCTTTGACAACGG
ACTGGCCGGTCCTTTCACCCGCGCCGTCGAGATCATCGGCGACATCTCTGGCCGGGTCAT
CGTTACCGGCGTCGGCAAGAGCGGCCATATCGGTACCAAGCTCGCGGCGACATTCGCCTC
GACCGGCACCCCCGCCTTTTTCGTGCATGCGGCCGAAGCCAATCATGGCGATCTCGGCAT
GATCGCACGCGATGACGTCGTCTTGGCGATTTCCAAAGGCGGCGAAAGCGCCGAGCTCAA
GAGTATCATTTCCTTCACTCGGCGCTTCTCGATCCCCTTGATCGCGATCACTTGCAGCGA
AGCTTCCTCGCTTGCGACAGCCGCCGACATCGTGCTTCTGGTGCCGAACGAACAGGAGGC
TTGCCCCAACGGGCTGGCGCCGACGACCTCGACGCTGATGCAGCTTGCACTTGGCGATGC
CTTGGCGGTCGCACTCCTGGAGGCGCGGGGCTTTACCGCCACCGATTTCCACGTTTTTCA
TCCCGGCGGCAAGCTGGGTGCGAGCCTGATGCATGTCGCCGATATCATGCATACCGGCGA
GAAGCTGCCGCTCGTTGTCAAGGGCACGCCGATGCCGGAGGCGATCACGGTGCTGTCGCG
CAAGCATTTCGGCTGCGTCGGCGTGCTCGACCCGGATGGGCGGCTATGCGGCATCGTCAC
CGAAGGCGACATGGCTCGGAACCTGAGCCGCAATCTTGCCGAGCTTGCCGTCGACGACAT
CATGACGAAAACGCCGAAGACGGTGAAACCGACGATGCTGGCGACCGCTGCCCTGGCGCT
GCTCAACCAGCACCACATTGGCGCCCTGATCGTCATCGACGAGGACAGCCGGCCGATCGG
GCTGGTGCACTTCCATGACTTGCTGCGGATCGGCGTCGCCTAACAGTAAAGGTAA

Protein sequence predicted based on nucleotide sequence

NRRAIKLVENSVLESAKRTIETEKHGLEALERAFDNGLAGPFTRAVEIIGDISGRVIVTG
VGKSGHIGTKLAATFASTGTPAFFVHAAEANHGDLGMIARDDVVLAISKGGESAELKSII
SFTRRFSIPLIAITCSEASSLATAADIVLLVPNEQEACPNGLAPTTSTLMQLALGDALAV
ALLEARGFTATDFHVFHPGGKLGASLMHVADIMHTGEKLPLVVKGTPMPEAITVLSRKHF
GCVGVLDPDGRLCGIVTEGDMARNLSRNLAELAVDDIMTKTPKTVKPTMLATAALALLNQ
HHIGALIVIDEDSRPIGLVHFHDLLRIGVA*

Coding Sequence Details

Insert Sequence Verified?:
Y
Verification Method:
Sequence Verification
Mutations:
None
Discrepancy:
None
5' Linker Sequence:
None
3' Linker Sequence:
None

Recommended Growth Condition:

Distributed in bacterial strain:
DH5-alpha T1 phage resistant
Antibiotic Selection:
Host Type:  bacterial    Marker:  kanamycin
Bacterial Selection Condition: 50ug/mL kanamycin
Growth Condition: Growth with the single antibiotic in LB at 37 degrees is recommended.
Comments: Conditions for Gateway-type vectors in recombined (with insert) form and other kanamycin resistant vectors.
Protein Expression Results:
SolubleProtein: Not_Applicable
ProteinExpressed: Protein_Confirmed
ProteinPurified: Protein_Purified
Find experimental protocols in NYSGRC-014442
Recommended expression in:
Not Applicable