dnaG (G. sulfurreducens PCA )
In pNIC28-Bsa4 (His-tagged bacterial expression vector)

Explanation of Terms

Gene:
Gene Symbol:  dnaG
Gene Name:  DNA primase
Original Clone ID:
013549
Keyword:
None
Species:
Geobacter sulfurreducens PCA
Type:
cDNA
Vector Name:
pNIC28-Bsa4
Format: 
CLOSED
Source:
New York Structural Genomics Research Consortium
Description:
None
Comments:
None
Mutation / Discrepancy:
No / No
Publications:
None
Authors:
Albert Einstein College of Medicine
Steven Almo
PSI:Biology
New York Structural Genomics Research Consortium
GsCD00526653
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AVAILABLE
No restriction
Special MTA: PSI
Insert sequence: 1761nts
Open reading frame: 22 to 1782

AACCTGTACTTCCAATCCATGTCAAACATCCCCGACGATAAGATTAGGGAGATACGCGAG
CGAGCTCCGATTCTGGAGGTGGTCTCGGATTATGTGAGCCTCCGGAAATCGGGCGCCAAT
TACCAGGGACTCTGTCCGTTCCACGGCGAGAAGACCCCCTCCTTCAACGTGAATCCGGCC
CGGGGGATCTTTCACTGTTTCGGTTGCGGCGTTGGCGGAAATGCCATCACCTTTGTCATG
AAGATGGAGGGACTGTCGTTTCCCGAAGCGGTCAAGTTCCTGGGCAAGCGGGTCGGTGTC
GAAATCGAGGACCGTCCCGTTACCGCCGCAGAGAAACGGCGGCAGGACGAACGCGAAGAG
CTCTATGGGATCATGGACCTGGCTGCCAGGCTCTATCGCGAGGCCCTGGAGCGAAACGAA
GAGGCCGGCGCGGCCCGGCGCTACCTTGAAACGCGCGGTGTGGACCGGGAGACGTCCGAG
GCGTACCGTCTCGGGTTCGCGCCGGACCGGTGGGATTATCTCTCGCGGCACCTGCAACAC
CGCAAGGTCTCCATGGATCTGGCGGAACGGCTGGGACTCGTGCGTCGGCGCGAGGGGGGC
GGCTACTTCGATATCTTCCGCAACCGTCTCTTGTTTGCCATCACCGATATCCACGGACGC
ACGATCGGGTTTGGCGGCAGAGTCCTGGATGACTCGCTGCCCAAATACATCAACTCTCCC
GAGTCGCCCATCTATCACAAGAGCGACGTGCTCTTCGGCGTCCACCTGGCAAAGGGCGCT
CTGCGGGAGCAGGGCAGCGCCATTATCGTAGAGGGTTATTTCGATCATCTTGCCCTCTAT
CAGGCAGGGGTCCGGAACGTGGTCGCCACTTGCGGCACGGCCTTGACCGAAGGCCATGTG
AAGCTTTTGCGGCGCTACGCCGGGAAAGCCTACACCCTGTTCGATGCGGATAGTGCCGGA
CGGAAGGCGACCGTGCGGGCGATCGACCTCTTTCTTGCGGAAAACTTCCCGGCCCACGTG
GTGGAGTTGACCCAGGGAGAAGACCCGGACAGTTTCGTCAAGAAAGAGGGGGCGGACATC
TTCCGGACACGGCTCGCCAAGGCCCGGCCGGCATTTGATTTTCTCTTCCGCGACCTGCTC
GGACAGGTGGATGCGGGTACGGTAGAGGGAAAGCTGAAGGTGGCGAACGAGTTGGCGCCC
CGCTTGGCAAAGCTCGCCAATCCCATCGAGCGGGAACTCTACGTGAAGGAGATCGCACGG
GTTCTGGGTATTGATGTCCGTTCCCTGCTGCGTGCGTCGGGCGGAGCAGCCGGATCACGC
CCTGACCCCCTTCCCCGCCCGACTGCGGAGCGCCGGCCACGTGGCGCCGGTCCCGAAGAG
ATGCTGCTTGCTCTCATGGCGCGATCCGCTGAAGTGGCAGATCGAGTCCGCCGCGGTGAG
GCGGCGGGGCTATTCCGTCCCGAGCTTGCTCCCGTTGCCGATGCCATACTTGAGCGGCAC
AGCCGGCGGGTGGAGATTGACTGGGGGGAGCTTCTCGAACTGGTTGCATCCCCCGAGGAA
AAGGGACGCATTGCGGCGTTCCTCGTCAACGACGATCATCTCGCGGACGTTGATGTCCAT
AAGGCCTTTGACCAGTGTCGCCAAGCCCTGGAGCGTGGTTCCCTCAAGCGGCTCAAGGAC
TTGGCCAGAGAGCTGGCTGCGGCTGATTCGGATACCCCCCGGTACCGGGAGCTGCTTGCC
GAAATAGAGGCCTTGCGAAACAGGAAATCGCAGTTATCTTAACAGTAAAGGTGG

Protein sequence predicted based on nucleotide sequence

SNIPDDKIREIRERAPILEVVSDYVSLRKSGANYQGLCPFHGEKTPSFNVNPARGIFHCF
GCGVGGNAITFVMKMEGLSFPEAVKFLGKRVGVEIEDRPVTAAEKRRQDEREELYGIMDL
AARLYREALERNEEAGAARRYLETRGVDRETSEAYRLGFAPDRWDYLSRHLQHRKVSMDL
AERLGLVRRREGGGYFDIFRNRLLFAITDIHGRTIGFGGRVLDDSLPKYINSPESPIYHK
SDVLFGVHLAKGALREQGSAIIVEGYFDHLALYQAGVRNVVATCGTALTEGHVKLLRRYA
GKAYTLFDADSAGRKATVRAIDLFLAENFPAHVVELTQGEDPDSFVKKEGADIFRTRLAK
ARPAFDFLFRDLLGQVDAGTVEGKLKVANELAPRLAKLANPIERELYVKEIARVLGIDVR
SLLRASGGAAGSRPDPLPRPTAERRPRGAGPEEMLLALMARSAEVADRVRRGEAAGLFRP
ELAPVADAILERHSRRVEIDWGELLELVASPEEKGRIAAFLVNDDHLADVDVHKAFDQCR
QALERGSLKRLKDLARELAAADSDTPRYRELLAEIEALRNRKSQLS*

Coding Sequence Details

Insert Sequence Verified?:
Y
Verification Method:
Sequence Verification
Mutations:
None
Discrepancy:
None
5' Linker Sequence:
None
3' Linker Sequence:
None

Recommended Growth Condition:

Distributed in bacterial strain:
DH5-alpha T1 phage resistant
Antibiotic Selection:
Host Type:  bacterial    Marker:  kanamycin
Bacterial Selection Condition: 50ug/mL kanamycin
Growth Condition: Growth with the single antibiotic in LB at 37 degrees is recommended.
Comments: Conditions for Gateway-type vectors in recombined (with insert) form and other kanamycin resistant vectors.
Protein Expression Results:
SolubleProtein: Not_Applicable
ProteinExpressed: Protein_Confirmed
ProteinPurified: Protein_Purified
Find experimental protocols in NYSGRC-013549
Recommended expression in:
Not Applicable