MCA1271 (M. capsulatus str. Bath )
In pNIC28-Bsa4 (His-tagged bacterial expression vector)

Explanation of Terms

Gene:
Gene Symbol:  MCA1271
Gene Name:  short chain dehydrogenase/reductase oxidoreductase
Original Clone ID:
014057
Keyword:
None
Species:
Methylococcus capsulatus str. Bath
Type:
cDNA
Vector Name:
pNIC28-Bsa4
Format: 
CLOSED
Source:
New York Structural Genomics Research Consortium
Description:
None
Comments:
None
Mutation / Discrepancy:
No / Yes
Publications:
None
Authors:
Albert Einstein College of Medicine
Steven Almo
PSI:Biology
New York Structural Genomics Research Consortium
McCD00526724
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AVAILABLE
No restriction
Special MTA: PSI
Insert sequence: 765nts
Open reading frame: 12 to 776

TCCAATCCATGAGCCCTGCAAACCCTGCACTCCACGGCCGCACCGTCTTGATCACCGGCG
CCGGGGGCGATCTCGGCGCCGTCGCCGCCGAGGCCTGTGCCGGTGCCGGCGCCACGGTGA
TCCTCCTGGGCCGTACCCTCGGACGGCTGGAAGCCACTTACGACCGCATCGTCCGGCAGG
GCGGCTCCGAACCACTCCTGGCGCCGCTGGACCTGGCCACCGCCACGCTCGAAGAATTGT
CGGCCATCGCCGGCTCGATCGAATCCCGGCTCGGCGCATTGCATGGGCTGATCCATTGCG
CCGCGCACATGGATTACCTGGGCCCTTTGCGGGATGTCGGCGACGCCCAGTGGCGCGATA
CGCTCGACGTCAACCTGACCGGCGCGTTCCGCCTGACACGGACTCTGTTGCCCCTGCTGA
CGCGCTCGGAAGCCGCCGCCGCGGTCTTCGTCAGTGACAGCGAAGCCGAGCGGCGGCGCA
GCTACTGGGGACCCTATGCCGTCGCCAAGGCCGGACTGGAAGCCTTCGCCCGGATTCTCG
CGGATGAATACGAAAGCGCCGGCACGCTCAGGGTCAACCTGCTCACGCCCGGCGCGATCC
GCACCCGCCTGCGGCGCAAGGCATTTCCCACGGAAACCGCGCCGGAACCCGGGGCGCTCG
CCGCCCGTTTCGTCGACCTGATGCGCAGCGACAGCCCCTACGCCAACGGCGAACGGATTG
CCGCCGCGGTCACCCCATCCGTCATCGAGGAGGAAATTCAGCATGTACGGACGTAACAGT
AAAGGTAA

Protein sequence predicted based on nucleotide sequence

SPANPALHGRTVLITGAGGDLGAVAAEACAGAGATVILLGRTLGRLEATYDRIVRQGGSE
PLLAPLDLATATLEELSAIAGSIESRLGALHGLIHCAAHMDYLGPLRDVGDAQWRDTLDV
NLTGAFRLTRTLLPLLTRSEAAAAVFVSDSEAERRRSYWGPYAVAKAGLEAFARILADEY
ESAGTLRVNLLTPGAIRTRLRRKAFPTETAPEPGALAARFVDLMRSDSPYANGERIAAAV
TPSVIEEEIQHVRT*

Coding Sequence Details

Insert Sequence Verified?:
Y
Verification Method:
Sequence Verification
Mutations:
None
Discrepancy:
c168g,H56Q;t540c,Y180Y
                                                    

5' Linker Sequence:
None
3' Linker Sequence:
None

Recommended Growth Condition:

Distributed in bacterial strain:
DH5-alpha T1 phage resistant
Antibiotic Selection:
Host Type:  bacterial    Marker:  kanamycin
Bacterial Selection Condition: 50ug/mL kanamycin
Growth Condition: Growth with the single antibiotic in LB at 37 degrees is recommended.
Comments: Conditions for Gateway-type vectors in recombined (with insert) form and other kanamycin resistant vectors.
Protein Expression Results:
SolubleProtein: Not_Applicable
ProteinExpressed: Tested_Not_Found
ProteinPurified: Tested_Not_Purified
Find experimental protocols in NYSGRC-014057
Recommended expression in:
Not Applicable