Gene:
Gene Symbol:
Sphk2
Gene Name:
sphingosine kinase 2
Original Clone ID:
12067
Keyword:
full-length cds
Species:
Mus musculus
Type:
cDNA
Vector Name:
pMH4
Format:
CLOSED
Source:
Joint Center for Structural Genomics
Description:
None
Comments:
None
Mutation / Discrepancy:
No / Yes
Publications:
None
Authors:
PSI
Joint Center for Structural Genomics
Joint Center for Structural Genomics
MmCD00530521
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AVAILABLE
No restriction
Special MTA: PSI
Special MTA: PSI
Insert sequence: 1854nts
Open reading frame: 51 to 1904
AGATATACCTACCCATGGGATCTGATAAAATTCATCATCATCATCATCACATGGCCCCAC
CACCACTACTGCCAGTGGCTGCCAGCACTCCAATCCTGCACGGCGAGTTTGGTTCCTACC
CGGCCAACGGCCCACGGTTTGCCCTCACCCTCACAACACAAGCCCTACACATACAGCGAC
TACGCCCAAAGCCAGAAGCCCGGCCCCGAGATGGTCTAGTCTCTCTGGATGAGGTCTCGG
GCTGTGGCACCCTGCAGAGCCGTAGCCCCGAGGACACTGCAGCCTACTTCTGCATCTACA
CCTACCCACGTGGCCGTCGAGGGGGCCGGCGCAGAGCTACGCGGACCTTCCGGGCGGATG
GGGCCACCACTTATGAGGAGAATCGTGCAGAGGCCCAGCGCTGGGCCACTGCCCTCACGT
GTCTCCTCCGAGGAGTGCCTCTGTCAGGGGACCAGGAAATCACCCCTGAATTGCTGCCCC
GGAAGCCCAGGCTGCTCATATTGGTCAATCCCTTTGGGGGGCGGGGCCTGGCCTGGCAGC
GCTGTATGGACCACGTGGTGCCAATGATCTCTGAAGCTGGGCTGTCCTTCAACCTCATAC
AGACAGAACGACAGAACCATGCCCGTGAGCTGGTGCAGGGGTTAAGCCTGAGTGAGTGGG
AAGGCATTGTCACTGTGTCTGGAGACGGGCTGCTTTACGAGGTGCTGAATGGGCTCCTTG
ATCGGCCAGACTGGGAGGATGCCGTGCGGATGCCCATTGGTGTCCTCCCCTGTGGATCGG
GCAATGCGCTAGCTGGGGCGGTGAACCATCATGGCGGGTTTGAGCAGGTTGTCGGTGTTG
ACCTGTTGCTCAACTGCTCGCTTCTTCTCTGCCGTGGTGGCAGCCATCCTCTGGACCTGC
TCTCTGTGACGCTAGCCTCGGGATCCCGCTGTTTTTCCTTCCTGTCAGTGGCCTGGGGAT
TCTTGTCAGATGTGGACATTCACAGTGAGCGCTTCAGGGCCCTGGGCAGCGCTCGATTCA
CACTGGGTGCAGTGCTAGGCCTGGCCTCGTTGCATACCTACCGTGGACGCCTCTCCTACC
TCCCCGCTACCACAGAACCAGCCTTGCCCATCCCAGGCCACAGTCTGCCTCGAGCCAAGT
CAGAACTAGTCTTGGCTCCAGCCCCAGCCCCCGCCGCCACCCACTCGCCTCTACATCGAT
CTGTGTCTGACCTGCCCCTGCCCCTTCCCCAGCCTGCCTTGGTCTCCCCTGGCTCCCCTG
AGCCCCTGCCTGACCTGTCCCTCAATGGTGGTGGTCCAGAGCTGACTGGAGACTGGGGAG
GAGCTGGGGATGCACCTCTGTCCCCAGACCCACTGCTGCCTTCATCCCCCAACGCTCTCA
AAACAGCTCAGCTTTCACCCATCGCTGAAGGGCCCCCAGAAATGCCAGCATCTTCGGGGT
TCCTGCCTCCCACCCACAGTGCCCCAGAAGCCTCTACCTGGGGCCCAGTGGACCACCTCC
TCCCTCCCCTGGGCTCTCCACTGCCCCAAGACTGGGTGACAATAGAGGGGGAGTTTGTAC
TCATGTTGGGCATCTTGCCGAGCCACCTCTGCGCAGACCTGATGGCAGCCCCACATGCAC
GCTTTGATGATGGCGTTGTGCACCTGTGTTGGGTGCGGAGCGGCATCTCACGGGCTGCAC
TTCTACGCATTTTGCTGGCCATGGAGCATGGAAACCACTTCAGCCTGGGCTGCCCCCATC
TGGGCTATGCTGCAGCACGTGCCTTCCGCCTTGAACCACTCACGCCTCGTGGCCTGCTCA
CTGTAGATGGGGAGTTAGTGGAGTATGGGCCAATACAGGCGCAGGTGCACCCAGGTCTCG
CCACGCTGCTCACTGGGCCTGCAGGTCAAAAGCCACAAGCCTGACGGGACTTAATTAACT
CTAGATAACTGAGAAGGAGATATACATACCCGGGT
Protein sequence predicted based on nucleotide sequence
MAPPPLLPVAASTPILHGEFGSYPANGPRFALTLTTQALHIQRLRPKPEARPRDGLVSLD EVSGCGTLQSRSPEDTAAYFCIYTYPRGRRGGRRRATRTFRADGATTYEENRAEAQRWAT ALTCLLRGVPLSGDQEITPELLPRKPRLLILVNPFGGRGLAWQRCMDHVVPMISEAGLSF NLIQTERQNHARELVQGLSLSEWEGIVTVSGDGLLYEVLNGLLDRPDWEDAVRMPIGVLP CGSGNALAGAVNHHGGFEQVVGVDLLLNCSLLLCRGGSHPLDLLSVTLASGSRCFSFLSV AWGFLSDVDIHSERFRALGSARFTLGAVLGLASLHTYRGRLSYLPATTEPALPIPGHSLP RAKSELVLAPAPAPAATHSPLHRSVSDLPLPLPQPALVSPGSPEPLPDLSLNGGGPELTG DWGGAGDAPLSPDPLLPSSPNALKTAQLSPIAEGPPEMPASSGFLPPTHSAPEASTWGPV DHLLPPLGSPLPQDWVTIEGEFVLMLGILPSHLCADLMAAPHARFDDGVVHLCWVRSGIS RAALLRILLAMEHGNHFSLGCPHLGYAAARAFRLEPLTPRGLLTVDGELVEYGPIQAQVH PGLATLLTGPAGQKPQA*
Coding Sequence Details
Insert Sequence Verified?:
Y
Verification Method:
Sequence Verification
Mutations:
None
Discrepancy:
None
5' Linker Sequence:
None
3' Linker Sequence:
None
Recommended Growth Condition:
Distributed in bacterial strain:
DH5-alpha T1 phage resistant
Antibiotic Selection:
Host Type:
bacterial
Marker:
ampicillin
Bacterial Selection Condition:
100 ug/mL ampicillin
Growth Condition:
Growth with the single antibiotic in LB at 37 degrees is recommended.
Comments:
Commonly used conditions for ampicillin resistant plasmid clones.
Protein Expression Results:
ProteinExpressed: Not_Applicable
ProteinPurified: Protein_Purified
SolubleProtein: Tested_Not_Soluble
Find experimental protocols in 354830
Recommended expression in:
Not Applicable
Vector Name:
pMH4
Synonyms:
pMH-4
Sequencing Primer:
Forward:
pBAD forward
Reverse:
alt_bad_rev
Description:
Bacterial expression vector, arabinose or T7 induced expression, adds N-terminal MGSDKIHHHHHH tag; amp resistance; restriction enyzme (Pml I/Pac I) or PIPE cloning.
Comments:
None
Size (bp):
5489
Parent Vector:
None
Properties:
PIPE cloning, bacterial expression, in vitro transcription, inducible expression, with tag/fusion/marker
Author Name:
Joint Center for Structural Genomics
Publications:
None
Vector Map:
Vector Sequence:
Sequence:
Vector Features:
Type
Name
Description
Start
End
bacterial origin
ori
ColE1 bacterial origin of replication
3331
4013
inducible trxn activator protein gene
araC
araC cds
5463
4585
negative selection marker
ccdB
ccdB negative selection gene (may be gone in with-insert form)
1576
1785
promoter
T7
T7 promoter
290
310
promoter
araC
araC promoter
153
124
selectable marker
CmR
chloramphenicol resistance gene (CmR)
484
1140
selectable marker
AmpR
ampicillin resistance gene
2574
3233
tag
His
N-terminal 6xHis tag
364
381
trxn regulatory element
arabinose O1 operator
arabinose O1 operator
161
182
trxn regulatory element
arabinose O2 operator
arabinose O2 operator
5
23
trxn termination sequence
rrnB
rrnB T2 transcription terminator
2238
2264
