MJ_1154 (M. jannaschii ) in pMH4 (bacterial expression vector)

Explanation of Terms

Gene:
Gene Symbol:  MJ_1154
Gene Name:  hypothetical protein
Original Clone ID:
15890
Keyword:
full-length cds
Species:
Methanocaldococcus jannaschii
Type:
cDNA
Vector Name:
pMH4
Format: 
CLOSED
Source:
Joint Center for Structural Genomics
Description:
None
Comments:
Linker not verified.
Mutation / Discrepancy:
No / No
Publications:
None
Authors:
PSI
Joint Center for Structural Genomics
MjCD00530680
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AVAILABLE
No restriction
Special MTA: PSI
Insert sequence: 1356nts
Open reading frame: 35 to 1390

GGGATCTGATAAAATTCATCATCATCATCATCACATGAAGGTTATTAGAGACTCTATCCA
CAAAGATATATATTTAGATGAAAAAGAGCTGGAGATTATTGATAGCGAAGAATTTCAGAG
ATTGAGAAATATAAAACAGACTGGTTTAACATACTTAGTTTATCCATCAGCAAATCATAC
AAGGTTTGAACATTCCTTAGGAACTATGTTTATTGCCTCAAAAATAGCAGAGAAGATTAA
TGCAGATGTTGAGCTTACAAGAGTCTCCGCTTTATTGCATGATATTGGACATCCTCCATT
CTCTCACACATTGGAAATTTGTGGCTACAGTCATGAAGTTTTTGGCAGAAAGAAAATCAA
ACATATGAATTTAGATAACTTTTCAAAGAGCGAAATAATTAAAACCTTAAATAGGAAAAA
TTTAGAGGGTAAGATAATTTCTGGAGATGTTGATGCTGATAGAATGGATTATTTATTGAG
GGATAGCTACCACACAGGAACAGCTTATGGGATGATTGATTTACCAAGAATTCTGAGGAG
TATAACAACCTTTGAGAGTTTTGGAAAAGTTAAGATAGGGATATTAAAGAAGGGAATTCA
AGCAATTGAATCGCTATTAGTTGCGAGGCATCAGATGTATTCAGCTGTTTATATGCATCC
AACAGTTAGAATAGCGGACACTATGATAAAGAGGGCAGTAATAAAAGAAATACAAGAAAA
AAATTTGGATATAAAAGATTTAGCTAACATGGATGATATTGCACTTGTTTCATTTTTGAG
GATTTCTGAAAACTATTTGATGGAGAGAATAGACAGGAGAAATCTCTATAAAAATCTCAT
CACCTATAGTTACTTTGATTTAAATCCAATAGAAAAATGGATTTTTGTCAATTTAGATGA
AAAACAAATATTATCATTAGAAAGTAGGTTTTATGAGGAATTCGGATGGGATATATTTAT
CGATATCTATCCAATTCCTAAAATGGAAGAGCATAACGTTTATATAATCTCAGATGAAGG
CGTTAAAAGATTGGATGAAGTTTCTCCATTAGCTCAGAGCTTAAAGCCCTCTGAGATGAG
ATTATGGAATATTTCAATCTATGCACCAAAAGAAAAAATTAAAGAGCTTAGAGAGAACAA
TGTAAAGGACAGGATAAATAAAATCTTAAAAGAGTTAGATGTTAAGGTTGAAAGCAAGTT
AATTGACATTTTGAAAGAATATGGGACAATTACTGGAAAGAGAAGATTTTTAGAGATTGC
TAAGGAAAGAGGCATTTCACCAAAAGAGTTTTACAATGAATTGCATAAATTGATATTCTG
CGGTTTAATAAAAGAGAGATTTAATAGGAGGACGTATGTTTATTGTTTAAATAATTTTGT
TAAATTATAACGCGACTTAATTAACTCTAGATAACTG

Protein sequence predicted based on nucleotide sequence

MKVIRDSIHKDIYLDEKELEIIDSEEFQRLRNIKQTGLTYLVYPSANHTRFEHSLGTMFI
ASKIAEKINADVELTRVSALLHDIGHPPFSHTLEICGYSHEVFGRKKIKHMNLDNFSKSE
IIKTLNRKNLEGKIISGDVDADRMDYLLRDSYHTGTAYGMIDLPRILRSITTFESFGKVK
IGILKKGIQAIESLLVARHQMYSAVYMHPTVRIADTMIKRAVIKEIQEKNLDIKDLANMD
DIALVSFLRISENYLMERIDRRNLYKNLITYSYFDLNPIEKWIFVNLDEKQILSLESRFY
EEFGWDIFIDIYPIPKMEEHNVYIISDEGVKRLDEVSPLAQSLKPSEMRLWNISIYAPKE
KIKELRENNVKDRINKILKELDVKVESKLIDILKEYGTITGKRRFLEIAKERGISPKEFY
NELHKLIFCGLIKERFNRRTYVYCLNNFVKL*

Coding Sequence Details

Insert Sequence Verified?:
Y
Verification Method:
Sequence Verification
Mutations:
None
Discrepancy:
None
5' Linker Sequence:
None
3' Linker Sequence:
None

Recommended Growth Condition:

Distributed in bacterial strain:
DH5-alpha T1 phage resistant
Antibiotic Selection:
Host Type:  bacterial    Marker:  ampicillin
Bacterial Selection Condition: 100 ug/mL ampicillin
Growth Condition: Growth with the single antibiotic in LB at 37 degrees is recommended.
Comments: Commonly used conditions for ampicillin resistant plasmid clones.
Protein Expression Results:
ProteinExpressed: Not_Applicable
ProteinPurified: Protein_Purified
SolubleProtein: Tested_Not_Soluble
Find experimental protocols in 357485
Recommended expression in:
Not Applicable