Gmet_2761 (G. metallireducens )
In pET21_NESG (His-tagged bacterial expression vector)

Explanation of Terms

Gene:
Gene Symbol:  Gmet_2761
Gene Name:  periplasmic substrate-binding sensor protein
Original Clone ID:
GmR31-21.4
Keyword:
None
Species:
Geobacter metallireducens
Type:
cDNA
Vector Name:
pET21_NESG
Format: 
FUSION
Source:
Northeast Structural Genomics Consortium
Description:
None
Comments:
None
Mutation / Discrepancy:
No / Yes
Publications:
None
Authors:
PSI
Northeast Structural Genomics Consortium
GmCD00530783
Price: 
Login for Pricing
AVAILABLE
No restriction
Special MTA: PSI/NESG
Insert sequence: 1218nts
Open reading frame: 32 to 1249

TATTTTGTTTACTTTAGAAGGAGATATACATATGAAGCATGGCGGTCCGATACGGGGGGC
GATCCTGCTCTTCGCTTTGTTTCTGGGAGCCGGGTCGGTCTTCGTGCGCCAGGTGGAAGA
AGGGCGTTGCCGCGAGCAGCGCCGGAACGTGGCCCAGCTCACCGCGGGTGCCGCCTTTTC
GCTGGAGCGCCAGATATCCGGCTCCCTCTCCGCCACCTACGCCCTTGCCGCCATTCTGCG
CCAGGCCGGCACCATTAGGGACTTTGATGCCCTCGCTGCCGAGATGATCCGGACCTACGG
CGGTATCAGCAGCCTGCAACTCGCCCCGGCCGGGGTCATGACCACCATCTACCCCCTTGC
CGGCAACGAAAAGGCCATAGGCCACGACCTCCTCAGCGACCCCCTCCGTCGCACCGAGGC
GCAACGGGCGATCGAGAGCCGGCAGATGACCCTTGCCGGCCCCTTCGAGCTGAGGCAAGG
GGGGGTAGGAGCCGTTGGCAGGCTGGCGGTTTTTCTCCCCGAACCGGGCAGGGGGGAGCG
TTTCTGGGGCTTCGTCAATGTCTTGATGCGCCTCCGGGACCTCCTCGCCGCAAGCAATCT
GGAAGCTCTCGATAAGGGGGGGTACGGGTATGAGCTCACCAGGCTCAACCCGGACACGGG
CCGGTGGGAATCCATTGCTCGCTCTGACGGACCGCTCCAGGCGCCGGTGGAAAGCACCAT
CGACGTCCCCAACGGCACCTGGAAGCTGGCCGTGGCGCCGAAACAGGGGTGGCAGACTTC
CCCGTTCCTTTACGTGGAATACCTCATGGTTGGCGGCATCAGCGGCATCCTGGCGCTTCT
CGCCTATCTCATGCTGAGACAACCGGAAAAGCTCCGCCACGAGGTCCTCCTGCGGACGGC
GGACCTGGAGAAGGCCAACCGGCAGCTGGAGGCGCAAATCCGGGAGCAGCAGCGTGCCGA
GGACGAGTTGCGCCGCCTCAATGCCGACCTTGAACTGCGGGTCACCGAGCGGACGGTGCA
GCTCGAGAACTCCAACCGGGAACTGGAGCCCTTCAGCTATTCGGTCTCCCATGACCTGCG
GGCCCCCCTGCGGCATATGGAGAGTTTCAGCCGCATCCTTGCGGAAGATCATGCCGGGCA
GATCGGCGAAGGGGGGGAGCAGTGCCTGGAGCGAATCGTGGCGGAAATCCAGCGCATGAA
ACTCCACATCGACAGCCTGCTGGAGCAGGCCCAGGTTGAAAGAATACGC

Protein sequence predicted based on nucleotide sequence

MKHGGPIRGAILLFALFLGAGSVFVRQVEEGRCREQRRNVAQLTAGAAFSLERQISGSLS
ATYALAAILRQAGTIRDFDALAAEMIRTYGGISSLQLAPAGVMTTIYPLAGNEKAIGHDL
LSDPLRRTEAQRAIESRQMTLAGPFELRQGGVGAVGRLAVFLPEPGRGERFWGFVNVLMR
LRDLLAASNLEALDKGGYGYELTRLNPDTGRWESIARSDGPLQAPVESTIDVPNGTWKLA
VAPKQGWQTSPFLYVEYLMVGGISGILALLAYLMLRQPEKLRHEVLLRTADLEKANRQLE
AQIREQQRAEDELRRLNADLELRVTERTVQLENSNRELEPFSYSVSHDLRAPLRHMESFS
RILAEDHAGQIGEGGEQCLERIVAEIQRMKLHIDSLLEQAQVERIR

Coding Sequence Details

Insert Sequence Verified?:
Y
Verification Method:
Sequence Verification
Mutations:
None
Discrepancy:
g130a,A44T;a227g,K76R;
c648t,A216A;g917a,R306Q;

t1018c,S340P
                                                    

5' Linker Sequence:
None
3' Linker Sequence:
None

Recommended Growth Condition:

Distributed in bacterial strain:
DH5-alpha T1 phage resistant
Antibiotic Selection:
Host Type:  bacterial    Marker:  ampicillin
Bacterial Selection Condition: 100 ug/mL ampicillin
Growth Condition: Growth with the single antibiotic in LB at 37 degrees is recommended.
Comments: Commonly used conditions for ampicillin resistant plasmid clones.
Protein Expression Results:
ProteinPurified: Not_Tested
ProteinExpressed: Protein_Confirmed
SolubleProtein: Protein_Soluble
Find experimental protocols in GmR31
Recommended expression in:
Not Applicable