LBA1314 (L. acidophilus )
In pET21_NESG (His-tagged bacterial expression vector)

Explanation of Terms

Gene:
Gene Symbol:  LBA1314
Gene Name:  pyrophosphokinase
Original Clone ID:
LaR32-21.2
Keyword:
None
Species:
Lactobacillus acidophilus
Type:
cDNA
Vector Name:
pET21_NESG
Format: 
FUSION
Source:
Northeast Structural Genomics Consortium
Description:
None
Comments:
None
Mutation / Discrepancy:
No / Yes
Publications:
None
Authors:
PSI
Northeast Structural Genomics Consortium
LaCD00530906
Price: 
Login for Pricing
AVAILABLE
No restriction
Special MTA: PSI/NESG
Insert sequence: 684nts
Open reading frame: 51 to 734

AATTCCCCTCTAGAAATAATTTTGTTTAACTTTAAGAAGGAGATATACATATGAAAGCAT
ATGCATTAGTAGGTGGGCCAACTGATTTGTGGCTACATGATATAAAAAAGCAGTTAACTG
AAGCTAAGCAGAATAATGATTTGATTTTTGGCGTTGATCGTGGTGCGTTGCTTTTAGAAG
AATTAGGTATTATCCCCGATGTAGCTATAGGTGATTTTGATTCGCTTCAAGCTAAAGATT
TATCTCGAATTGAAAAGACAGTTAAGGACATTAGATATTCTAATCCGATAAAAGACTGGA
CTGATTCTGAAATAATGGTGCAAACGGTCTTTAAAGACTATCTAGCAGATAAATTAATAA
TTTTAGGTGCCAGTGGTGGAAGAATAGATCACTTTTTAATTAATTTATTAATGTGGTTAA
ATCCTTCGATTAATCAATTTGCTCAAAGAGTTGAAATAATTGATAAACAAAATAGTATTG
TGTTTTTTAACCCTGGCGTCCATATAATTAAAAAAAAGCCAGATTATCCGTACATTGGTT
TTGCTACATTGTCAGAAACTGAAGACTTTAATATAAGTGGTGCGAGATATGATTTAAATG
ATTATTCAAGTACATATCCTCGAGTTTTTTCTTCAAATGAATTTTTACCTAATAGCGATT
ATTTTGAAATATCATTAAAAAAAGGAATGATTGCAGCAATTTATTCTAAAGATATTAATC
GCTTTCATAATTTACTCGAGCACCACCACCACCACCACGAGATCCGGCTG

Protein sequence predicted based on nucleotide sequence

MKAYALVGGPTDLWLHDIKKQLTEAKQNNDLIFGVDRGALLLEELGIIPDVAIGDFDSLQ
AKDLSRIEKTVKDIRYSNPIKDWTDSEIMVQTVFKDYLADKLIILGASGGRIDHFLINLL
MWLNPSINQFAQRVEIIDKQNSIVFFNPGVHIIKKKPDYPYIGFATLSETEDFNISGARY
DLNDYSSTYPRVFSSNEFLPNSDYFEISLKKGMIAAIYSKDINRFHNL

Coding Sequence Details

Insert Sequence Verified?:
Y
Verification Method:
Sequence Verification
Mutations:
None
Discrepancy:
t121c,F41L;c376t,P126S;
c417a,N139K;c420a,H140Q
                                                    

5' Linker Sequence:
None
3' Linker Sequence:
None

Recommended Growth Condition:

Distributed in bacterial strain:
DH5-alpha T1 phage resistant
Antibiotic Selection:
Host Type:  bacterial    Marker:  ampicillin
Bacterial Selection Condition: 100 ug/mL ampicillin
Growth Condition: Growth with the single antibiotic in LB at 37 degrees is recommended.
Comments: Commonly used conditions for ampicillin resistant plasmid clones.
Protein Expression Results:
ProteinExpressed: Protein_Confirmed
ProteinPurified: Protein_Purified
SolubleProtein: Protein_Soluble
Find experimental protocols in LaR32
Recommended expression in:
Not Applicable