DNASU Plasmid | CDC14A (H. sapiens )<br>In pFASTBAC1-C-His-TOPO (His-tagged baculovirus expression vector)

Explanation of Terms

Gene:

Gene Symbol: CDC14A

Gene Name: cell division cycle 14A

Original Clone ID:

8650a5KWt1

Keyword:

None

Species:

Homo sapiens

Type:

cDNA

Vector Name:

pFASTBAC1-C-His-TOPO

Format:

CLOSED

Source:

New York SGX Research Center for Structural Genomics

Description:

None

Comments:

Linker not verified.

Mutation / Discrepancy:

No / No

Publications:

None

Authors:

PSI
New York SGX Research Center for Structural Genomics

HsCD00531166

Price:

AVAILABLE

No restriction
Special MTA: PSI

Insert Sequence
Protein Sequence

Insert sequence: 1065nts

Open reading frame: 51 to 1115

Reference Sequence Alignment

GTCCCACCATCGGGCGCGGATCCCGGTCCGAAGCGCGCGGAATTCGCACCATGGCCCTTG
CGGAGTCAGGGGAACTAATCGGGGCTTGTGAGTTCATGAAAGATCGGTTATATTTTGCTA
CTTTAAGGAATAGACCAAAAAGCACAGTAAATACCCACTATTTCTCCATCGATGAGGAGC
TGGTCTATGAAAATTTCTATGCAGATTTTGGACCGCTGAACTTGGCAATGGTGTACAGAT
ATTGCTGCAAACTAAACAAGAAACTAAAATCATACAGTTTGTCAAGAAAGAAAATAGTGC
ACTACACCTGTTTTGACCAACGGAAAAGAGCAAATGCAGCATTTTTGATAGGTGCCTATG
CAGTAATCTATTTAAAGAAGACACCAGAAGAAGCCTACAGAGCACTCCTGTCTGGCTCAA
ACCCCCCCTATCTTCCATTCAGGGATGCTTCCTTTGGAAATTGCACTTACAATCTCACCA
TTCTCGACTGTTTGCAGGGAATCAGAAAGGGATTACAACATGGATTTTTTGACTTTGAGA
CATTTGATGTGGATGAATATGAACATTATGAGCGAGTTGAAAATGGTGACTTCAACTGGA
TTGTTCCAGGAAAATTTTTAGCATTTAGTGGACCACATCCTAAAAGCAAAATTGAGAATG
GTTATCCTCTTCACGCCCCTGAAGCCTACTTTCCTTATTTCAAAAAGCATAATGTGACTG
CAGTTGTGAGGCTAAACAAAAAGATTTATGAGGCAAAGCGCTTCACAGACGCTGGCTTCG
AGCACTATGACCTCTTCTTCATAGATGGCAGCACACCCAGTGACAACATCGTGCGAAGGT
TCCTGAACATCTGTGAGAACACCGAAGGGGCCATCGCCGTTCACTGCAAAGCTGGTCTTG
GAAGAACAGGGACATTGATAGCCTGTTATGTAATGAAACACTACAGGTTTACACATGCTG
AAATAATTGCTTGGATTAGAATATGCCGGCCAGGCTCTATTATAGGACCCCAGCAGCACT
TCCTGGAAGAAAAACAAGCATCGTTGTGGGTCCAAGGAGACATTTTCCGATCCAAACTGA
AAAATCGAGAAGGGCATCATCACCATCACCACTGA

Protein sequence predicted based on nucleotide sequence

MALAESGELIGACEFMKDRLYFATLRNRPKSTVNTHYFSIDEELVYENFYADFGPLNLAM
VYRYCCKLNKKLKSYSLSRKKIVHYTCFDQRKRANAAFLIGAYAVIYLKKTPEEAYRALL
SGSNPPYLPFRDASFGNCTYNLTILDCLQGIRKGLQHGFFDFETFDVDEYEHYERVENGD
FNWIVPGKFLAFSGPHPKSKIENGYPLHAPEAYFPYFKKHNVTAVVRLNKKIYEAKRFTD
AGFEHYDLFFIDGSTPSDNIVRRFLNICENTEGAIAVHCKAGLGRTGTLIACYVMKHYRF
THAEIIAWIRICRPGSIIGPQQHFLEEKQASLWVQGDIFRSKLKNREGHHHHHH*

Coding Sequence Details

Insert Sequence Verified?:

Verification Method:

Sequence Verification

Mutations:

None

Discrepancy:

None

5' Linker Sequence:

None

3' Linker Sequence:

None

Recommended Growth Condition:

Distributed in bacterial strain:

DH5-alpha T1 phage resistant

Antibiotic Selection:

Host Type: bacterial Marker: ampicillin

Bacterial Selection Condition: 100 ug/mL ampicillin

Growth Condition: Growth with the single antibiotic in LB at 37 degrees is recommended.

Comments: Commonly used conditions for ampicillin resistant plasmid clones.

Protein Expression Results:

ProteinExpressed: Not_Tested

ProteinPurified: Not_Tested

SolubleProtein: Not_Tested

Find experimental protocols in NYSGXRC-8650a

Recommended expression in:

Not Applicable

Vector Name:

pFASTBAC1-C-His-TOPO

Synonyms:

pFB1-C-His-TOPO, pFB1-C-His-TOPO_KW

Sequencing Primer:

Forward: pFB forward

Reverse: pFB reverse

Description:

Baculovirus vector, adds C-terminal 6xHis tag and TEV protease site; amp resistance; TOPO cloning.

Comments:

None

Size (bp):

4763

Parent Vector:

None

Properties:

TOPO Cloning, baculovirus/insect cell expression, with tag/fusion/marker

Author Name:

New York SGX Research Center for Structural Genomics

Publications:

None

Vector Map:

Vector Sequence:

Sequence:

GACGCGCCCTGTAGCGGCGCATTAAGCGCGGCGGGTGTGGTGGTTACGCGCAGCGTGACCGCTACACTTGCCAGCGCCCTAGCGCCCGCTCCTTTCGCTTTCTTCCCTTCCTTTCTCGCCACGTTCGCCGGCTTTCCCCGTCAAGCTCTAAATCGGGGGCTCCCTTTAGGGTTCCGATTTAGTGCTTTACGGCACCTCGACCCCAAAAAACTTGATTAGGGTGATGGTTCACGTAGTGGGCCATCGCCCTGATAGACGGTTTTTCGCCCTTTGACGTTGGAGTCCACGTTCTTTAATAGTGGACTCTTGTTCCAAACTGGAACAACACTCAACCCTATCTCGGTCTATTCTTTTGATTTATAAGGGATTTTGCCGATTTCGGCCTATTGGTTAAAAAATGAGCTGATTTAACAAAAATTTAACGCGAATTTTAACAAAATATTAACGTTTACAATTTCAGGTGGCACTTTTCGGGGAAATGTGCGCGGAACCCCTATTTGTTTATTTTTCTAAATACATTCAAATATGTATCCGCTCATGAGACAATAACCCTGATAAATGCTTCAATAATATTGAAAAAGGAAGAGTATGAGTATTCAACATTTCCGTGTCGCCCTTATTCCCTTTTTTGCGGCATTTTGCCTTCCTGTTTTTGCTCACCCAGAAACGCTGGTGAAAGTAAAAGATGCTGAAGATCAGTTGGGTGCACGAGTGGGTTACATCGAACTGGATCTCAACAGCGGTAAGATCCTTGAGAGTTTTCGCCCCGAAGAACGTTTTCCAATGATGAGCACTTTTAAAGTTCTGCTATGTGGCGCGGTATTATCCCGTATTGACGCCGGGCAAGAGCAACTCGGTCGCCGCATACACTATTCTCAGAATGACTTGGTTGAGTACTCACCAGTCACAGAAAAGCATCTTACGGATGGCATGACAGTAAGAGAATTATGCAGTGCTGCCATAACCATGAGTGATAACACTGCGGCCAACTTACTTCTGACAACGATCGGAGGACCGAAGGAGCTAACCGCTTTTTTGCACAACATGGGGGATCATGTAACTCGCCTTGATCGTTGGGAACCGGAGCTGAATGAAGCCATACCAAACGACGAGCGTGACACCACGATGCCTGTAGCAATGGCAACAACGTTGCGCAAACTATTAACTGGCGAACTACTTACTCTAGCTTCCCGGCAACAATTAATAGACTGGATGGAGGCGGATAAAGTTGCAGGACCACTTCTGCGCTCGGCCCTTCCGGCTGGCTGGTTTATTGCTGATAAATCTGGAGCCGGTGAGCGTGGGTCTCGCGGTATCATTGCAGCACTGGGGCCAGATGGTAAGCCCTCCCGTATCGTAGTTATCTACACGACGGGGAGTCAGGCAACTATGGATGAACGAAATAGACAGATCGCTGAGATAGGTGCCTCACTGATTAAGCATTGGTAACTGTCAGACCAAGTTTACTCATATATACTTTAGATTGATTTAAAACTTCATTTTTAATTTAAAAGGATCTAGGTGAAGATCCTTTTTGATAATCTCATGACCAAAATCCCTTAACGTGAGTTTTCGTTCCACTGAGCGTCAGACCCCGTAGAAAAGATCAAAGGATCTTCTTGAGATCCTTTTTTTCTGCGCGTAATCTGCTGCTTGCAAACAAAAAAACCACCGCTACCAGCGGTGGTTTGTTTGCCGGATCAAGAGCTACCAACTCTTTTTCCGAAGGTAACTGGCTTCAGCAGAGCGCAGATACCAAATACTGTCCTTCTAGTGTAGCCGTAGTTAGGCCACCACTTCAAGAACTCTGTAGCACCGCCTACATACCTCGCTCTGCTAATCCTGTTACCAGTGGCTGCTGCCAGTGGCGATAAGTCGTGTCTTACCGGGTTGGACTCAAGACGATAGTTACCGGATAAGGCGCAGCGGTCGGGCTGAACGGGGGGTTCGTGCACACAGCCCAGCTTGGAGCGAACGACCTACACCGAACTGAGATACCTACAGCGTGAGCATTGAGAAAGCGCCACGCTTCCCGAAGGGAGAAAGGCGGACAGGTATCCGGTAAGCGGCAGGGTCGGAACAGGAGAGCGCACGAGGGAGCTTCCAGGGGGAAACGCCTGGTATCTTTATAGTCCTGTCGGGTTTCGCCACCTCTGACTTGAGCGTCGATTTTTGTGATGCTCGTCAGGGGGGCGGAGCCTATGGAAAAACGCCAGCAACGCGGCCTTTTTACGGTTCCTGGCCTTTTGCTGGCCTTTTGCTCACATGTTCTTTCCTGCGTTATCCCCTGATTCTGTGGATAACCGTATTACCGCCTTTGAGTGAGCTGATACCGCTCGCCGCAGCCGAACGACCGAGCGCAGCGAGTCAGTGAGCGAGGAAGCGGAAGAGCGCCTGATGCGGTATTTTCTCCTTACGCATCTGTGCGGTATTTCACACCGCAGACCAGCCGCGTAACCTGGCAAAATCGGTTACGGTTGAGTAATAAATGGATGCCCTGCGTAAGCGGGTGTGGGCGGACAATAAAGTCTTAAACTGAACAAAATAGATCTAAACTATGACAATAAAGTCTTAAACTAGACAGAATAGTTGTAAACTGAAATCAGTCCAGTTATGCTGTGAAAAAGCATACTGGACTTTTGTTATGGCTAAAGCAAACTCTTCATTTTCTGAAGTGCAAATTGCCCGTCGTATTAAAGAGGGGCGTGGCCAAGGGCATGGTAAAGACTATATTCGCGGCGTTGTGACAATTTACCGAACAACTCCGCGGCCGGGAAGCCGATCTCGGCTTGAACGAATTGTTAGGTGGCGGTACTTGGGTCGATATCAAAGTGCATCACTTCTTCCCGTATGCCCAACTTTGTATAGAGAGCCACTGCGGGATCGTCACCGTAATCTGCTTGCACGTAGATCACATAAGCACCAAGCGCGTTGGCCTCATGCTTGAGGAGATTGATGAGCGCGGTGGCAATGCCCTGCCTCCGGTGCTCGCCGGAGACTGCGAGATCATAGATATAGATCTCACTACGCGGCTGCTCAAACCTGGGCAGAACGTAAGCCGCGAGAGCGCCAACAACCGCTTCTTGGTCGAAGGCAGCAAGCGCGATGAATGTCTTACTACGGAGCAAGTTCCCGAGGTAATCGGAGTCCGGCTGATGTTGGGAGTAGGTGGCTACGTCTCCGAACTCACGACCGAAAAGATCAAGAGCAGCCCGCATGGATTTGACTTGGTCAGGGCCGAGCCTACATGTGCGAATGATGCCCATACTTGAGCCACCTAACTTTGTTTTAGGGCGACTGCCCTGCTGCGTAACATCGTTGCTGCTGCGTAACATCGTTGCTGCTCCATAACATCAAACATCGACCCACGGCGTAACGCGCTTGCTGCTTGGATGCCCGAGGCATAGACTGTACAAAAAAACAGTCATAACAAGCCATGAAAACCGCCACTGCGCCGTTACCACCGCTGCGTTCGGTCAAGGTTCTGGACCAGTTGCGTGAGCGCATACGCTACTTGCATTACAGTTTACGAACCGAACAGGCTTATGTCAACTGGGTTCGTGCCTTCATCCGTTTCCACGGTGTGCGTCACCCGGCAACCTTGGGCAGCAGCGAAGTCGAGGCATTTCTGTCCTGGCTGGCGAACGAGCGCAAGGTTTCGGTCTCCACGCATCGTCAGGCATTGGCGGCCTTGCTGTTCTTCTACGGCAAGGTGCTGTGCACGGATCTGCCCTGGCTTCAGGAGATCGGAAGACCTCGGCCGTCGCGGCGCTTGCCGGTGGTGCTGACCCCGGATGAAGTGGTTCGCATCCTCGGTTTTCTGGAAGGCGAGCATCGTTTGTTCGCCCAGGACTCTAGCTATAGTTCTAGTGGTTGGCTACGTATACTCCGGAATATTAATAGATCATGGAGATAATTAAAATGATAACCATCTCGCAAATAAATAAGTATTTTACTGTTTTCGTAACAGTTTTGTAATAAAAAAACCTATAAATATTCCGGATTATTCATACCGTCCCACCATCGGGCGCGGATCCCGGTCCGAAGCGCGCGGAATTCGCACCATGGCCCTTAAGGGCATCATCACCATCACCACTGATAGCTCGAGGCATGCGGTACCAAGCTTGTCGAGAAGTACTAGAGGATCATAATCAGCCATACCACATTTGTAGAGGTTTTACTTGCTTTAAAAAACCTCCCACACCTCCCCCTGAACCTGAAACATAAAATGAATGCAATTGTTGTTGTTAACTTGTTTATTGCAGCTTATAATGGTTACAAATAAAGCAATAGCATCACAAATTTCACAAATAAAGCATTTTTTTCACTGCATTCTAGTTGTGGTTTGTCCAAACTCATCAATGTATCTTATCATGTCTGGATCTGATCACTGCTTGAGCCTAGGAGATCCGAACCAGATAAGTGAAATCTAGTTCCAAACTATTTTGTCATTTTTAATTTTCGTATTAGCTTACGACGCTACACCCAGTTCCCATCTATTTTGTCACTCTTCCCTAAATAATCCTTAAAAACTCCATTTCCACCCCTCCCAGTTCCCAACTATTTTGTCCGCCCACAGCGGGGCATTTTTCTTCCTGTTATGTTTTTAATCAAACATCCTGCCAACTCCATGTGACAAACCGTCATCTTCGGCTACTTTTTCTCTGTCACAGAATGAAAATTTTTCTGTCATCTCTTCGTTATTAATGTTTGTAATTGACTGAATATCAACGCTTATTTGCAGCCTGAATGGCGAATGG

Vector Features:

Type

Name

Description

Start

End

ATG start

start

initiation ATG (Met, Ala, Leu)

4065

4078

TOPO recognition site

TOPO

TOPO cloning site

4069

4078

bacterial origin

ori

pUC-type bacterial origin of replication

1544

2226

poly-A signal

polyA

SV40 polyA signal sequence

4189

4380

primer

pFB foward

forward sequencing primer: �TATTCCGGATTATTCATACC

3995

4014

primer

pFB reverse

reverse sequencing primer: ACAAATGTGGTATGGCTGATT

4150

4170

promoter

polyhedrin promoter for insect cell expression

3904

4032

selectable marker

GenR

gentamycin resistance gene

3000

3533

selectable marker

AmpR

ampicillin resistance gene

787

1446

ssDNA origin

f1 ori

f1 origin

325

tag

His

C-terminal 6xHis and stop

4079

4096

transposon fragment

Tn7L

Tn7L transposon site

1466

transposon fragment

Tn7R

Tn7R transposon site

2709

2933

Gene

Gene Symbol :

CDC14A

Symbol Nomenclature :

CDC14A

SYNONYM :

cdc14; hCDC14

Designation :

CDC10 (cell division cycle 10, S. cerevisiae, homolog)|CDC14 cell division cycle 14 homolog A|dual specificity protein phosphatase CDC14A

Full Nomenclature :

cell division cycle 14A

GENEID :

8556

GI :

15451929

GenBank Accession :

NM_003672

HGNC :

1718

MIM :

603504

Vega :

OTTHUMG00000010987

Target GenBank:

None

Reference Sequence Alignment

  

HsCD00531166        GTCCCACCATCGGGCGCGGATCCCGGTCCGAAGCGCGCGGAATTCGCACCATGGCCCTTG
NM_033313.2         -----------------------------------------------------ATGGCAG
NM_003672.3         -----------------------------------------------------ATGGCAG
XM_005271296.1      --------------------------------------------------ATGGGAAACT
XM_005271294.1      --------------------------------------------------ATGGGAAACT
NM_033312.2         -----------------------------------------------------ATGGCAG
                                                                         .      

HsCD00531166        CGGAGTCAGGGGAACTAATCGGGGCTTGTGAGTTCATGAAAGATCGGTTATATTTTGCTA
NM_033313.2         CGGAGTCAGGGGAACTAATCGGGGCTTGTGAGTTCATGAAAGATCGGTTATATTTTGCTA
NM_003672.3         CGGAGTCAGGGGAACTAATCGGGGCTTGTGAGTTCATGAAAGATCGGTTATATTTTGCTA
XM_005271296.1      TTTTGTCCCGTGAGAACAAAGTACAAGTTATATCAGAGTCTGATCGGTTATATTTTGCTA
XM_005271294.1      TTTTGTCCCGTGAGAACAAAGTACAAGTTATATCAGAGTCTGATCGGTTATATTTTGCTA
NM_033312.2         CGGAGTCAGGGGAACTAATCGGGGCTTGTGAGTTCATGAAAGATCGGTTATATTTTGCTA
                       :***. * **..:.*:.* . .:  *.:.* ..:*:.:*******************

HsCD00531166        CTTTAAGGAATAGACCAAAAAGCACAGTAAATACCCACTATTTCTCCATCGATGAGGAGC
NM_033313.2         CTTTAAGGAATAGACCAAAAAGCACAGTAAATACCCACTATTTCTCCATCGATGAGGAGC
NM_003672.3         CTTTAAGGAATAGACCAAAAAGCACAGTAAATACCCACTATTTCTCCATCGATGAGGAGC
XM_005271296.1      CTTTAAGGAATAGACCAAAAAGCACAGTAAATACCCACTATTTCTCCATCGATGAGGAGC
XM_005271294.1      CTTTAAGGAATAGACCAAAAAGCACAGTAAATACCCACTATTTCTCCATCGATGAGGAGC
NM_033312.2         CTTTAAGGAATAGACCAAAAAGCACAGTAAATACCCACTATTTCTCCATCGATGAGGAGC
                    ************************************************************

HsCD00531166        TGGTCTATGAAAATTTCTATGCAGATTTTGGACCGCTGAACTTGGCAATGGTGTACAGAT
NM_033313.2         TGGTCTATGAAAATTTCTATGCAGATTTTGGACCGCTGAACTTGGCAATGGTGTACAGAT
NM_003672.3         TGGTCTATGAAAATTTCTATGCAGATTTTGGACCGCTGAACTTGGCAATGGTGTACAGAT
XM_005271296.1      TGGTCTATGAAAATTTCTATGCAGATTTTGGACCGCTGAACTTGGCAATGGTGTACAGAT
XM_005271294.1      TGGTCTATGAAAATTTCTATGCAGATTTTGGACCGCTGAACTTGGCAATGGTGTACAGAT
NM_033312.2         TGGTCTATGAAAATTTCTATGCAGATTTTGGACCGCTGAACTTGGCAATGGTGTACAGAT
                    ************************************************************

HsCD00531166        ATTGCTGCAAACTAAACAAGAAACTAAAATCATACAGTTTGTCAAGAAAGAAAATAGTGC
NM_033313.2         ATTGCTGCAAACTAAACAAGAAACTAAAATCATACAGTTTGTCAAGAAAGAAAATAGTGC
NM_003672.3         ATTGCTGCAAACTAAACAAGAAACTAAAATCATACAGTTTGTCAAGAAAGAAAATAGTGC
XM_005271296.1      ATTGCTGCAAACTAAACAAGAAACTAAAATCATACAGTTTGTCAAGAAAGAAAATAGTGC
XM_005271294.1      ATTGCTGCAAACTAAACAAGAAACTAAAATCATACAGTTTGTCAAGAAAGAAAATAGTGC
NM_033312.2         ATTGCTGCAAACTAAACAAGAAACTAAAATCATACAGTTTGTCAAGAAAGAAAATAGTGC
                    ************************************************************

HsCD00531166        ACTACACCTGTTTTGACCAACGGAAAAGAGCAAATGCAGCATTTTTGATAGGTGCCTATG
NM_033313.2         ACTACACCTGTTTTGACCAACGGAAAAGAGCAAATGCAGCATTTTTGATAGGTGCCTATG
NM_003672.3         ACTACACCTGTTTTGACCAACGGAAAAGAGCAAATGCAGCATTTTTGATAGGTGCCTATG
XM_005271296.1      ACTACACCTGTTTTGACCAACGGAAAAGAGCAAATGCAGCATTTTTGATAGGTGCCTATG
XM_005271294.1      ACTACACCTGTTTTGACCAACGGAAAAGAGCAAATGCAGCATTTTTGATAGGTGCCTATG
NM_033312.2         ACTACACCTGTTTTGACCAACGGAAAAGAGCAAATGCAGCATTTTTGATAGGTGCCTATG
                    ************************************************************

HsCD00531166        CAGTAATCTATTTAAAGAAGACACCAGAAGAAGCCTACAGAGCACTCCTGTCTGGCTCAA
NM_033313.2         CAGTAATCTATTTAAAGAAGACACCAGAAGAAGCCTACAGAGCACTCCTGTCTGGCTCAA
NM_003672.3         CAGTAATCTATTTAAAGAAGACACCAGAAGAAGCCTACAGAGCACTCCTGTCTGGCTCAA
XM_005271296.1      CAGTAATCTATTTAAAGAAGACACCAGAAGAAGCCTACAGAGCACTCCTGTCTGGCTCAA
XM_005271294.1      CAGTAATCTATTTAAAGAAGACACCAGAAGAAGCCTACAGAGCACTCCTGTCTGGCTCAA
NM_033312.2         CAGTAATCTATTTAAAGAAGACACCAGAAGAAGCCTACAGAGCACTCCTGTCTGGCTCAA
                    ************************************************************

HsCD00531166        ACCCCCCCTATCTTCCATTCAGGGATGCTTCCTTTGGAAATTGCACTTACAATCTCACCA
NM_033313.2         ACCCCCCCTATCTTCCATTCAGGGATGCTTCCTTTGGAAATTGCACTTACAATCTCACCA
NM_003672.3         ACCCCCCCTATCTTCCATTCAGGGATGCTTCCTTTGGAAATTGCACTTACAATCTCACCA
XM_005271296.1      ACCCCCCCTATCTTCCATTCAGGGATGCTTCCTTTGGAAATTGCACTTACAATCTCACCA
XM_005271294.1      ACCCCCCCTATCTTCCATTCAGGGATGCTTCCTTTGGAAATTGCACTTACAATCTCACCA
NM_033312.2         ACCCCCCCTATCTTCCATTCAGGGATGCTTCCTTTGGAAATTGCACTTACAATCTCACCA
                    ************************************************************

HsCD00531166        TTCTCGACTGTTTGCAGGGAATCAGAAAGGGATTACAACATGGATTTTTTGACTTTGAGA
NM_033313.2         TTCTCGACTGTTTGCAGGGAATCAGAAAGGGATTACAACATGGATTTTTTGACTTTGAGA
NM_003672.3         TTCTCGACTGTTTGCAGGGAATCAGAAAGGGATTACAACATGGATTTTTTGACTTTGAGA
XM_005271296.1      TTCTCGACTGTTTGCAGGGAATCAGAAAGGGATTACAACATGGATTTTTTGACTTTGAGA
XM_005271294.1      TTCTCGACTGTTTGCAGGGAATCAGAAAGGGATTACAACATGGATTTTTTGACTTTGAGA
NM_033312.2         TTCTCGACTGTTTGCAGGGAATCAGAAAGGGATTACAACATGGATTTTTTGACTTTGAGA
                    ************************************************************

HsCD00531166        CATTTGATGTGGATGAATATGAACATTATGAGCGAGTTGAAAATGGTGACTTCAACTGGA
NM_033313.2         CATTTGATGTGGATGAATATGAACATTATGAGCGAGTTGAAAATGGTGACTTCAACTGGA
NM_003672.3         CATTTGATGTGGATGAATATGAACATTATGAGCGAGTTGAAAATGGTGACTTCAACTGGA
XM_005271296.1      CATTTGATGTGGATGAATATGAACATTATGAGCGAGTTGAAAATGGTGACTTCAACTGGA
XM_005271294.1      CATTTGATGTGGATGAATATGAACATTATGAGCGAGTTGAAAATGGTGACTTCAACTGGA
NM_033312.2         CATTTGATGTGGATGAATATGAACATTATGAGCGAGTTGAAAATGGTGACTTCAACTGGA
                    ************************************************************

HsCD00531166        TTGTTCCAGGAAAATTTTTAGCATTTAGTGGACCACATCCTAAAAGCAAAATTGAGAATG
NM_033313.2         TTGTTCCAGGAAAATTTTTAGCATTTAGTGGACCACATCCTAAAAGCAAAATTGAGAATG
NM_003672.3         TTGTTCCAGGAAAATTTTTAGCATTTAGTGGACCACATCCTAAAAGCAAAATTGAGAATG
XM_005271296.1      TTGTTCCAGGAAAATTTTTAGCATTTAGTGGACCACATCCTAAAAGCAAAATTGAGAATG
XM_005271294.1      TTGTTCCAGGAAAATTTTTAGCATTTAGTGGACCACATCCTAAAAGCAAAATTGAGAATG
NM_033312.2         TTGTTCCAGGAAAATTTTTAGCATTTAGTGGACCACATCCTAAAAGCAAAATTGAGAATG
                    ************************************************************

HsCD00531166        GTTATCCTCTTCACGCCCCTGAAGCCTACTTTCCTTATTTCAAAAAGCATAATGTGACTG
NM_033313.2         GTTATCCTCTTCACGCCCCTGAAGCCTACTTTCCTTATTTCAAAAAGCATAATGTGACTG
NM_003672.3         GTTATCCTCTTCACGCCCCTGAAGCCTACTTTCCTTATTTCAAAAAGCATAATGTGACTG
XM_005271296.1      GTTATCCTCTTCACGCCCCTGAAGCCTACTTTCCTTATTTCAAAAAGCATAATGTGACTG
XM_005271294.1      GTTATCCTCTTCACGCCCCTGAAGCCTACTTTCCTTATTTCAAAAAGCATAATGTGACTG
NM_033312.2         GTTATCCTCTTCACGCCCCTGAAGCCTACTTTCCTTATTTCAAAAAGCATAATGTGACTG
                    ************************************************************

HsCD00531166        CAGTTGTGAGGCTAAACAAAAAGATTTATGAGGCAAAGCGCTTCACAGACGCTGGCTTCG
NM_033313.2         CAGTTGTGAGGCTAAACAAAAAGATTTATGAGGCAAAGCGCTTCACAGACGCTGGCTTCG
NM_003672.3         CAGTTGTGAGGCTAAACAAAAAGATTTATGAGGCAAAGCGCTTCACAGACGCTGGCTTCG
XM_005271296.1      CAGTTGTGAGGCTAAACAAAAAGATTTATGAGGCAAAGCGCTTCACAGACGCTGGCTTCG
XM_005271294.1      CAGTTGTGAGGCTAAACAAAAAGATTTATGAGGCAAAGCGCTTCACAGACGCTGGCTTCG
NM_033312.2         CAGTTGTGAGGCTAAACAAAAAGATTTATGAGGCAAAGCGCTTCACAGACGCTGGCTTCG
                    ************************************************************

HsCD00531166        AGCACTATGACCTCTTCTTCATAGATGGCAGCACACCCAGTGACAACATCGTGCGAAGGT
NM_033313.2         AGCACTATGACCTCTTCTTCATAGATGGCAGCACACCCAGTGACAACATCGTGCGAAGGT
NM_003672.3         AGCACTATGACCTCTTCTTCATAGATGGCAGCACACCCAGTGACAACATCGTGCGAAGGT
XM_005271296.1      AGCACTATGACCTCTTCTTCATAGATGGCAGCACACCCAGTGACAACATCGTGCGAAGGT
XM_005271294.1      AGCACTATGACCTCTTCTTCATAGATGGCAGCACACCCAGTGACAACATCGTGCGAAGGT
NM_033312.2         AGCACTATGACCTCTTCTTCATAGATGGCAGCACACCCAGTGACAACATCGTGCGAAGGT
                    ************************************************************

HsCD00531166        TCCTGAACATCTGTGAGAACACCGAAGGGGCCATCGCCGTTCACTGCAAAGCTGGTCTTG
NM_033313.2         TCCTGAACATCTGTGAGAACACCGAAGGGGCCATCGCCGTTCACTGCAAAGCTGGTCTTG
NM_003672.3         TCCTGAACATCTGTGAGAACACCGAAGGGGCCATCGCCGTTCACTGCAAAGCTGGTCTTG
XM_005271296.1      TCCTGAACATCTGTGAGAACACCGAAGGGGCCATCGCCGTTCACTGCAAAGCTGGTCTTG
XM_005271294.1      TCCTGAACATCTGTGAGAACACCGAAGGGGCCATCGCCGTTCACTGCAAAGCTGGTCTTG
NM_033312.2         TCCTGAACATCTGTGAGAACACCGAAGGGGCCATCGCCGTTCACTGCAAAGCTGGTCTTG
                    ************************************************************

HsCD00531166        GAAGAACAGGGACATTGATAGCCTGTTATGTAATGAAACACTACAGGTTTACACATGCTG
NM_033313.2         GAAGAACAGGGACATTGATAGCCTGTTATGTAATGAAACACTACAGGTTTACACATGCTG
NM_003672.3         GAAGAACAGGGACATTGATAGCCTGTTATGTAATGAAACACTACAGGTTTACACATGCTG
XM_005271296.1      GAAGAACAGGGACATTGATAGCCTGTTATGTAATGAAACACTACAGGTTTACACATGCTG
XM_005271294.1      GAAGAACAGGGACATTGATAGCCTGTTATGTAATGAAACACTACAGGTTTACACATGCTG
NM_033312.2         GAAGAACAGGGACATTGATAGCCTGTTATGTAATGAAACACTACAGGTTTACACATGCTG
                    ************************************************************

HsCD00531166        AAATAATTGCTTGGATTAGAATATGCCGGCCAGGCTCTATTATAGGACCCCAGCAGCACT
NM_033313.2         AAATAATTGCTTGGATTAGAATATGCCGGCCAGGCTCTATTATAGGACCCCAGCAGCACT
NM_003672.3         AAATAATTGCTTGGATTAGAATATGCCGGCCAGGCTCTATTATAGGACCCCAGCAGCACT
XM_005271296.1      AAATAATTGCTTGGATTAGAATATGCCGGCCAGGCTCTATTATAGGACCCCAGCAGCACT
XM_005271294.1      AAATAATTGCTTGGATTAGAATATGCCGGCCAGGCTCTATTATAGGACCCCAGCAGCACT
NM_033312.2         AAATAATTGCTTGGATTAGAATATGCCGGCCAGGCTCTATTATAGGACCCCAGCAGCACT
                    ************************************************************

HsCD00531166        TCCTGGAAGAAAAACAAGCATCGTTGTGGGTCCAAGGAGACATTTTCCGATCCAAACTGA
NM_033313.2         TCCTGGAAGAAAAACAAGCATCGTTGTGGGTCCAAGGAGACATTTTCCGATCCAAACTGA
NM_003672.3         TCCTGGAAGAAAAACAAGCATCGTTGTGGGTCCAAGGAGACATTTTCCGATCCAAACTGA
XM_005271296.1      TCCTGGAAGAAAAACAAGCATCGTTGTGGGTCCAAGGAGACATTTTCCGATCCAAACTGA
XM_005271294.1      TCCTGGAAGAAAAACAAGCATCGTTGTGGGTCCAAGGAGACATTTTCCGATCCAAACTGA
NM_033312.2         TCCTGGAAGAAAAACAAGCATCGTTGTGGGTCCAAGGAGACATTTTCCGATCCAAACTGA
                    ************************************************************

HsCD00531166        AAAATCGAGAAGGGCATCATCACCATCACCACTGA-------------------------
NM_033313.2         AAAATCGACCATCCAGTGAAGGAAGTATTAATAAAATTCTTTCTGGCCTAGATGATATGT
NM_003672.3         AAAATCGACCATCCAGTGAAGGAAGTATTAATAAAATTCTTTCTGGCCTAGATGATATGT
XM_005271296.1      AAAATCGACCATCCAGTGAAGGAAGTATTAATAAAATTCTTTCTGGCCTAGATGATATGT
XM_005271294.1      AAAATCGACCATCCAGTGAAGGAAGTATTAATAAAATTCTTTCTGGCCTAGATGATATGT
NM_033312.2         AAAATCGACCATCCAGTGAAGGAAGTATTAATAAAATTCTTTCTGGCCTAGATGATATGT
                    ******** .*   ..* *: ....*.: .* :.*                         

HsCD00531166        ------------------------------------------------------------
NM_033313.2         CTATTGGTGGAAATCTTTCAAAAACACAAAACATGGAACGATTTGGAGAGGTAAGTTTTC
NM_003672.3         CTATTGGTGGAAATCTTTCAAAAACACAAAACATGGAACGATTTGGAGAGGATAACTTAG
XM_005271296.1      CTATTGGTGGAAATCTTTCAAAAACACAAAACATGGAACGATTTGGAGAGGATAACTTAG
XM_005271294.1      CTATTGGTGGAAATCTTTCAAAAACACAAAACATGGAACGATTTGGAGAGGATAACTTAG
NM_033312.2         CTATTGGTGGAAATCTTTCAAAAACACAAAACATGGAACGATTTGGAGAGGATAACTTAG
                                                                                

HsCD00531166        ------------------------------------------------------------
NM_033313.2         CCTAG-------------------------------------------------------
NM_003672.3         AAGATGATGATGTGGAAATGAAAAATGGTATAACCCAGGGAGACAAACTACGTGCCTTAA
XM_005271296.1      AAGATGATGATGTGGAAATGAAAAATGGTATAACCCAGGGAGACAAACTACGTGCCTTAA
XM_005271294.1      AAGATGATGATGTGGAAATGAAAAATGGTATAACCCAGGGAGACAAACTACGTGCCTTAA
NM_033312.2         AAGATGATGATGTGGAAATGAAAAATGGTATAACCCAGGGAGACAAACTACGTGCCTTAA
                                                                                

HsCD00531166        ------------------------------------------------------------
NM_033313.2         ------------------------------------------------------------
NM_003672.3         AAAGTCAGAGACAGCCACGTACCTCACCATCCTGTGCATTTAGGTCAGATGATACAAAAG
XM_005271296.1      AAAGTCAGAGACAGCCACGTACCTCACCATCCTGTGCATTTAGGTCAGATGATACAAAAG
XM_005271294.1      AAAGTCAGAGACAGCCACGTACCTCACCATCCTGTGCATTTAGGTCAGATGATACAAAAG
NM_033312.2         AAAGTCAGAGACAGCCACGTACCTCACCATCCTGTGCATTTAGGTCAGATGATACAAAAG
                                                                                

HsCD00531166        ------------------------------------------------------------
NM_033313.2         ------------------------------------------------------------
NM_003672.3         GACATCCAAGAGCAGTGTCCCAGCCTTTCAGATTAAGTTCATCCCTGCAAGGATCTGCAG
XM_005271296.1      GACATCCAAGAGCAGTGTCCCAGCCTTTCAGATTAAGTTCATCCCTGCAAGGATCTGCAG
XM_005271294.1      GACATCCAAGAGCAGTGTCCCAGCCTTTCAGATTAAGTTCATCCCTGCAAGGATCTGCAG
NM_033312.2         GACATCCAAGAGCAGTGTCCCAGCCTTTCAGATTAAGTTCATCCCTGCAAGGATCTGCAG
                                                                                

HsCD00531166        ------------------------------------------------------------
NM_033313.2         ------------------------------------------------------------
NM_003672.3         TTACTTTGAAGACATCAAAAATGGCACTGTCCCCTTCAGCAACGGCCAAGAGGATCAACA
XM_005271296.1      TTACTTTGAAGACATCAAAAATGGCACTGTCCCCTTCAGCAACGGCCAAGAGGATCAACA
XM_005271294.1      TTACTTTGAAGACATCAAAAATGGCACTGTCCCCTTCAGCAACGGCCAAGAGGATCAACA
NM_033312.2         TTACTTTGAAGACATCAAAAATGGCACTGTCCCCTTCAGCAACGGCCAAGAGGATCAACA
                                                                                

HsCD00531166        ------------------------------------------------------------
NM_033313.2         ------------------------------------------------------------
NM_003672.3         GAACTTCTTTGTCTTCGGGTGCCACTGTAAGAAGCTTTTCCATAAACTCCCGGCTAGCCA
XM_005271296.1      GAACTTCTTTGTCTTCGGGTGCCACTGTAAGAAGCTTTTCCATAAACTCCCGGCTAGCCA
XM_005271294.1      GAACTTCTTTGTCTTCGGGTGCCACTGTAAGAAGCTTTTCCATAAACTCCCGGCTAGCCA
NM_033312.2         GAACTTCTTTGTCTTCGGGTGCCACTGTAAGAAGCTTTTCCATAAACTCCCGGCTAGCCA
                                                                                

HsCD00531166        ------------------------------------------------------------
NM_033313.2         ------------------------------------------------------------
NM_003672.3         GTTCTCTAGGGAACTTGAATGCTGCAACAGATGATCCAGAGAACAAAAAGACCTCCTCAT
XM_005271296.1      GTTCTCTAGGGAACTTGAATGCTGCAACAGATGATCCAGAGAACAAAAAGACCTCCTCAT
XM_005271294.1      GTTCTCTAGGGAACTTGAATGCTGCAACAGATGATCCAGAGAACAAAAAGACCTCCTCAT
NM_033312.2         GTTCTCTAGGGAACTTGAATGCTGCAACAGATGATCCAGAGAACAAAAAGACCTCCTCAT
                                                                                

HsCD00531166        ------------------------------------------------------------
NM_033313.2         ------------------------------------------------------------
NM_003672.3         CCTCTAAGGCAGGCTTCACAGCCAGCCCGTTTACCAACCTCTTGAATGGCAGCTCCCAGC
XM_005271296.1      CCTCTAAGGCAGGCTTCACAGCCAGCCCGTTTACCAACCTCTTGAATGGCAGCTCCCAGC
XM_005271294.1      CCTCTAAGGCAGGCTTCACAGCCAGCCCGTTTACCAACCTCTTGAATGGCAGCTCCCAGC
NM_033312.2         CCTCTAAGGCAGGCTTCACAGCCAGCCCGTTTACCAACCTCTTGAATGGCAGCTCCCAGC
                                                                                

HsCD00531166        ------------------------------------------------------------
NM_033313.2         ------------------------------------------------------------
NM_003672.3         CAACTACCAGAAATTACCCTGAGCTCAACAATAATCAGTACAACAGAAGCAGCAACAGCA
XM_005271296.1      CAACTACCAGAAATTACCCTGAGCTCAACAATAATCAGTACAACAGAAGCAGCAACAGCA
XM_005271294.1      CAACTACCAGAAATTACCCTGAGCTCAACAATAATCAGTACAACAGAAGCAGCAACAGCA
NM_033312.2         CAACTACCAGAAATTACCCTGAGCTCAACAATAATCAGTACAACAGAAGCAGCAACAGCA
                                                                                

HsCD00531166        ------------------------------------------------------------
NM_033313.2         ------------------------------------------------------------
NM_003672.3         ACGGGGGCAACCTGAACAGCCCCCCAGGCCCCCACAGCGCCAAGACAGAGGAGCACACCA
XM_005271296.1      ACGGGGGCAACCTGAACAGCCCCCCAGGCCCCCACAGCGCCAAGACAGAGGAGCACACCA
XM_005271294.1      ACGGGGGCAACCTGAACAGCCCCCCAGGCCCCCACAGCGCCAAGACAGAGGAGCACACCA
NM_033312.2         ACGGGGGCAACCTGAACAGCCCCCCAGGCCCCCACAGCGCCAAGACAGAGGAGCACACCA
                                                                                

HsCD00531166        ------------------------------------------------------------
NM_033313.2         ------------------------------------------------------------
NM_003672.3         CCATCCTCCGACCCTCCTACACCGGGCTTTCTTCTTCTTCAGCGAGATTCCTGAGCCGTT
XM_005271296.1      CCATCCTCCGACCCTCCTACACCGGGCTTTCTTCTTCTTCAGCGAGATTCCTGAGCCGTT
XM_005271294.1      CCATCCTCCGACCCTCCTACACCGGGCTTTCTTCTTCTTCAGCGAGATTCCTGAGCCGTT
NM_033312.2         CCATCCTCCGACCCTCCTACACCGGGCTTTCTTCTTCTTCAGCGAGATTCCTGAGCCGTT
                                                                                

HsCD00531166        ------------------------------------------------------------
NM_033313.2         ------------------------------------------------------------
NM_003672.3         CTATCCCTTCCCTTCAGTCTGAATATGTTCATTACTAA----------------------
XM_005271296.1      CTATCCCTTCCCTTCAGTCTGAATATGTTCATTACTAA----------------------
XM_005271294.1      CTATCCCTGTAAGTGCGCAGACACCACCTCCTGGTCCTCAGAACCCTGAATGCAACTTCT
NM_033312.2         CTATCCCTGTAAGTGCGCAGACACCACCTCCTGGTCCTCAGAACCCTGAATGCAACTTCT
                                                                                

HsCD00531166        ------------------------------------------------------------
NM_033313.2         ------------------------------------------------------------
NM_003672.3         ------------------------------------------------------------
XM_005271296.1      ------------------------------------------------------------
XM_005271294.1      GTGCCTTGCCTTCCCAGCCGAGGCTGCCACCAAAGAAATTTAATAGTGCCAAGGAAGCCT
NM_033312.2         GTGCCTTGCCTTCCCAGCCGAGGCTGCCACCAAAGAAATTTAATAGTGCCAAGGAAGCCT
                                                                                

HsCD00531166        -----
NM_033313.2         -----
NM_003672.3         -----
XM_005271296.1      -----
XM_005271294.1      TCTGA
NM_033312.2         TCTGA

Pathways

Reactome :

APC/C-mediated degradation of cell cycle proteins

Reactome :

Cell Cycle

Reactome :

Cell Cycle, Mitotic

Reactome :

Conversion from APC/C:Cdc20 to APC/C:Cdh1 in late anaphase

Reactome :

Regulation of mitotic cell cycle

WikiPathway :

Cell cycle

Protein

SMART domain :

DSPc : Dual specificity phosphatase, catalytic domain

SMART domain :

PTPc_motif : Protein tyrosine phosphatase, catalytic domain motif

Structural Annotation Wiki :

NYSGXRC-8650a

HPRD :

04614

Clone

Cloning Information :

552127

HIP Master Clone ID :

462447

Original Clone ID :

8650a5KWt1

Experimental

TargetTrack - Experimental data :

NYSGXRC-8650a

Organism

Species Specific ID:

8556

Genome

Chromosome :

Map Location :

1p21

Ensembl :

ENSG00000079335

Reagents

Labome :

CDC14A-antibody

CDC14A (H. sapiens )In pFASTBAC1-C-His-TOPO (His-tagged baculovirus expression vector)

Coding Sequence Details

Recommended Growth Condition:

Vector Features:

Gene

Reference Sequence Alignment

Pathways

Protein

Clone

Experimental

Organism

Genome

Reagents

CDC14A (H. sapiens )
In pFASTBAC1-C-His-TOPO (His-tagged baculovirus expression vector)