BH3500 (H. halodurans )
In pET21_NESG (His-tagged bacterial expression vector)

Explanation of Terms

Gene:
Gene Symbol:  BH3500
Gene Name:  epidermal surface antigen
Original Clone ID:
BhR104-21.3
Keyword:
None
Species:
Halalkalibacterium halodurans
Type:
cDNA
Vector Name:
pET21_NESG
Format: 
FUSION
Source:
Northeast Structural Genomics Consortium
Description:
None
Comments:
None
Mutation / Discrepancy:
No / No
Publications:
None
Authors:
PSI
Northeast Structural Genomics Consortium
BhCD00531244
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AVAILABLE
No restriction
Special MTA: PSI/NESG
Insert sequence: 1554nts
Open reading frame: 1 to 1554

ATGACGGTTGTGACGTTTCTTGCTTTTTTAATCCCCATTCTTGTGCTTGCAGCCATTGGC
GGAGTGGTTTGGTGGTTCTGGATGAAGCTTCGCTACAAAACGGCGAGGTCGAACGAAGCG
CTCATTATTGCAGGGCCGAAGTTAGGTGATCCAAAGAAGGAAACGAATATTTTTACGGAT
AATGAAGGGCGGTCCATTAAAATCATTCGTGGCGGAGGACACCTTCTTAAGATGCACCAA
ACGGCAACCCCTGTTGATTTAACATCGTTTCAATTAAAATTAACGACGCCACGTGTCTTT
ACAAATGGTGGTGTTCCGATCATTGCTGATGCGGTTGCGATGGTGACGGTATCCGATACG
TTAAAAGGAATCGCCATTTATGCTGAGCAATTTTTAGGCAAGGAACAAAAGGAAATCGAA
AGTGAAATCTCAGAAGTGCTGAATGCAAATTTACGCGCGATTTTATCGAAAATGACGGTG
GAACAAATCAATGAAGATCGCGAAGGCTTTAACTTACAAGTGACCGAAGTGGCGCAAAAA
CAATTAGATAGCATGGGCTTCAAAATTACGTCATTAGGGTTACAAGATTTACGAGATGCC
GATAAAGAAAACGGCTATTTAGAAAATTTAGGTCGCCCACGTATTGCGGAAGTCCGCAAA
CGGGCCGAAATTGCTGAGTCGAATAGTGATAAAGAAACTCGAATTCATAAAGCGAATAAT
GATAAAGAAGCGAAAGAACAAGAGTTTCAACGGAAAATTGAAATTGCTGCGGCGTTAAAG
GAAAAAGACTTGAAAGACGCAGCGATTAAAGAAGAAACGGAACGGGCGAGAGCAAAAGCA
GAGCAATCCTATTTGTTAGAGAAAGCGAAGTTGGATAAAGAGGTACAAGAAGAGGAATTA
CAGCTCCTTGCTCGGAAAAAAGAAGAAGAATTGCGTATTAAGCATCTTGAACGGGAACGC
GCGGTAAAGCTAGAGGAAGAAGAAGCAAAAGTGCGTAGAGCAAAAGCAGATGCCGATTTC
TATGAGACGACGAGAAAGGCGGAAGCAGACGCGGAAAAAGCACGGATTGAAGGGGAAACA
AAAGCGAGAATCAAGCGGGAAGAAGGTTTGGCAGAAGCGGAAGTTATCCGTAAACGCGGG
GAAGCTGAAGCCGAAGCGAAGCGATTACTTGCAGAGGCGATTGCGAAACACGGTGAAGTC
ATTATCATTGAAAAACTTATCGAAATGTTACCGCAATTTGCTGAATCGATTTCAAAACCT
CTATCAAATATTGAATCGGTCAAAATTATCGACACAGGCAATGGCAACGGGGTGTCGTCC
TACAGCAAGAGCATTACGAACACGATGGCCCAAATCCAAGAGCCATTAAAAGAGTTAACA
GGACTGGATGTTTCTAAGCTGTTAACCGATCTTGTGAACCGAGGAAATACTCACACGGTC
GTTCGCACAGAAGCTGTGCCAACTGATCATCACGGGAGCCCCGTAGCGGCATCAACGGAA
TCGGAAGAGCCAGTAGAAGATCAACCGCTTGAGAACAGTGACAGTGAAGAAAAA

Protein sequence predicted based on nucleotide sequence

MTVVTFLAFLIPILVLAAIGGVVWWFWMKLRYKTARSNEALIIAGPKLGDPKKETNIFTD
NEGRSIKIIRGGGHLLKMHQTATPVDLTSFQLKLTTPRVFTNGGVPIIADAVAMVTVSDT
LKGIAIYAEQFLGKEQKEIESEISEVLNANLRAILSKMTVEQINEDREGFNLQVTEVAQK
QLDSMGFKITSLGLQDLRDADKENGYLENLGRPRIAEVRKRAEIAESNSDKETRIHKANN
DKEAKEQEFQRKIEIAAALKEKDLKDAAIKEETERARAKAEQSYLLEKAKLDKEVQEEEL
QLLARKKEEELRIKHLERERAVKLEEEEAKVRRAKADADFYETTRKAEADAEKARIEGET
KARIKREEGLAEAEVIRKRGEAEAEAKRLLAEAIAKHGEVIIIEKLIEMLPQFAESISKP
LSNIESVKIIDTGNGNGVSSYSKSITNTMAQIQEPLKELTGLDVSKLLTDLVNRGNTHTV
VRTEAVPTDHHGSPVAASTESEEPVEDQPLENSDSEEK

Coding Sequence Details

Insert Sequence Verified?:
Y
Verification Method:
Sequence Verification
Mutations:
None
Discrepancy:
None
5' Linker Sequence:
None
3' Linker Sequence:
None

Recommended Growth Condition:

Distributed in bacterial strain:
DH5-alpha T1 phage resistant
Antibiotic Selection:
Host Type:  bacterial    Marker:  ampicillin
Bacterial Selection Condition: 100 ug/mL ampicillin
Growth Condition: Growth with the single antibiotic in LB at 37 degrees is recommended.
Comments: Commonly used conditions for ampicillin resistant plasmid clones.
Protein Expression Results:
ProteinPurified: Not_Tested
ProteinExpressed: Protein_Confirmed
SolubleProtein: Protein_Soluble
Find experimental protocols in BhR104
Recommended expression in:
Not Applicable