MM_2469 (M. mazei )
In pET21_NESG (His-tagged bacterial expression vector)

Explanation of Terms

Gene:
Gene Symbol:  MM_2469
Gene Name:  hypothetical protein
Original Clone ID:
MaR194-21.2
Keyword:
None
Species:
Methanosarcina mazei
Type:
cDNA
Vector Name:
pET21_NESG
Format: 
FUSION
Source:
Northeast Structural Genomics Consortium
Description:
None
Comments:
None
Mutation / Discrepancy:
No / No
Publications:
None
Authors:
PSI
Northeast Structural Genomics Consortium
MmCD00531282
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AVAILABLE
No restriction
Special MTA: PSI/NESG
Insert sequence: 1482nts
Open reading frame: 51 to 1532

GGTGAGAATTCCTCTAGATAATTTTGTTTACTTTAGAAGGAGATATACATATGAATCATT
TATCGGGAATAAATTTAATTCTGGCACTGGGTCTGTTTTTTTGCTTTATTTCGAGTGCTG
AAGCCGCAGACCCACTTGCTTTGGATGACGGAGATGTGAATCTTTCGGAACTCAGGGCTT
TTGACCTGCTGGGCAGATGTGAAACAGGGTCTATAGTAAATACTGTATCACTTTCTTCTA
CAGGCAATTATCTTGCAGCCGGAGGGTTTGATCACAAAGTTTATTATTATGATTCCAGGG
GGACGCAACTATGGAATTATACTACAGGAGATATTGTTTATACTGTATCGATTTCTTCAG
ATGGATCTCGAATTGCAGCAGGAAGCAATGATAGAAAAGTGTACTTCTTTAACCGGGAAG
GAGAACTCCTGTGGAGCCGAAAAACCGGAGGCAATATTAATAGTGTAGCAGTCTCTTCTG
AAGGGCTTTACATTGCAACAGGCGGCGAGGACGGAAAGGTATATTTCCTGGACAAAGATG
GTAAACTTTTATGGAGCTTTAATTCAGGAGATGCTGTCCGCAGCGTAGCTATTTCCAGAG
ACGGGGATTACGTGGCAACAGGAAGCGCTAATAATTATGTTTACCTCCTTGACAGTGAGG
GAAAAACCCTCTGGGGGAAAAAAACAGGAAGCCTGATCAACAGTGTAACCATGACCCCTA
ATGCTTACTATGTGGCTGCAGGAGGGTCCAATTATAACGTTTATCTTTTTGACCGTAAAG
GGGAATTTTCATGGATGCATAATCCCGGTTACTGGGTCAGCAGTGTTTCTTTAAGCAGCA
ATGGATCCCATCTAGCTGCAGGAAGTTTCGACGACAGGATTTACCTCTTCAACCTTACAG
GCGCAAAACTATGGGACTACCAGGTAAAAGATGACGTTTATGCTGTGGAGATAGCGCCGG
ATTCATCTTTCATTGCAGCCGGGAGCTGGGACGATAGCATCTATGCACTTAATATGAGCG
GAAAAGAACTCTGGAACTGCAGCTGCGGAGGGAACATCAACAGCCTGGATGTTTCACGCG
ACAGTTCGACCATTGCTGCAGGGAGTGAAGCAGGAGCAGTATATATATTTAAACGGAACG
CAACCGCTTTTGCACTGCTATCCGGGGATGCAAGTTTCCTGCCAGAAGAGCCTGTAAAGA
CAGAAGATTCCTCTCTTGAAGAAAATATCCAGCCCGTAGAAGAAACCCCCGTAGATACAG
GAGGTATTGAAAACCTGGTAGAAGATGCTGCAGCTTCATCAGGTGATAAGAAAGAAAACA
TAAAAACCGGGGGTAAGCTGGGAATGGAGGAGTTGCCTTCAGAAAAAAGTTCTTCAAGCA
TTTTTGGGATATTTGATTCAATAAAGTTTCCGCTTGCCCTTCTGATCGGGTCCATGGCAG
GTACGGTTTTCTTCTTAAAAAGAAGGCTTACCAAACGTCAGGAGATGGATGAGAATACCG
AGGACTTTGAAAACCTTGAAGAGAGTGATACA

Protein sequence predicted based on nucleotide sequence

MNHLSGINLILALGLFFCFISSAEAADPLALDDGDVNLSELRAFDLLGRCETGSIVNTVS
LSSTGNYLAAGGFDHKVYYYDSRGTQLWNYTTGDIVYTVSISSDGSRIAAGSNDRKVYFF
NREGELLWSRKTGGNINSVAVSSEGLYIATGGEDGKVYFLDKDGKLLWSFNSGDAVRSVA
ISRDGDYVATGSANNYVYLLDSEGKTLWGKKTGSLINSVTMTPNAYYVAAGGSNYNVYLF
DRKGEFSWMHNPGYWVSSVSLSSNGSHLAAGSFDDRIYLFNLTGAKLWDYQVKDDVYAVE
IAPDSSFIAAGSWDDSIYALNMSGKELWNCSCGGNINSLDVSRDSSTIAAGSEAGAVYIF
KRNATAFALLSGDASFLPEEPVKTEDSSLEENIQPVEETPVDTGGIENLVEDAAASSGDK
KENIKTGGKLGMEELPSEKSSSSIFGIFDSIKFPLALLIGSMAGTVFFLKRRLTKRQEMD
ENTEDFENLEESDT

Coding Sequence Details

Insert Sequence Verified?:
Y
Verification Method:
Sequence Verification
Mutations:
None
Discrepancy:
None
5' Linker Sequence:
None
3' Linker Sequence:
None

Recommended Growth Condition:

Distributed in bacterial strain:
DH5-alpha T1 phage resistant
Antibiotic Selection:
Host Type:  bacterial    Marker:  ampicillin
Bacterial Selection Condition: 100 ug/mL ampicillin
Growth Condition: Growth with the single antibiotic in LB at 37 degrees is recommended.
Comments: Commonly used conditions for ampicillin resistant plasmid clones.
Protein Expression Results:
ProteinPurified: Not_Tested
ProteinExpressed: Protein_Confirmed
SolubleProtein: Protein_Soluble
Find experimental protocols in MaR194
Recommended expression in:
Not Applicable