Gene:
Gene Symbol:
PTPRK
Gene Name:
protein tyrosine phosphatase, receptor type, K
Original Clone ID:
8618a1ZBt4
Keyword:
full-length cds
Species:
Homo sapiens
Type:
cDNA
Vector Name:
pCR4zeroBLUNT-TOPO
Format:
FUSION
Source:
New York SGX Research Center for Structural Genomics
Description:
None
Comments:
None
Mutation / Discrepancy:
No / No
Publications:
None
Authors:
PSI
New York SGX Research Center for Structural Genomics
New York SGX Research Center for Structural Genomics
HsCD00531350
Price:
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AVAILABLE
No restriction
Special MTA: PSI
Special MTA: PSI
TACGACTCACTATAGGGCGAATTGAATTTAGCGGCCGCGAATTCGCCCTTCGCAAAGATG
CCATGGGGAATACCCGGCAGGAGATGACTCACATGGTGAATGCAATGGATCGAAGTTATG
CTGATCAGAGCACTCTGCATGCAGAAGATCCTCTTTCCATCACCTTCATGGACCAACATA
ACTTTAGTCCAAGATATGAGAACCACAGTGCTACAGCAGAGTCCAGTCGCCTTCTAGACG
TACCTCGCTACCTCTGTGAGGGGACGGAATCCCCTTACCAGACAGGACAGCTGCATCCAG
CCATCAGGGTAGCTGATTTACTGCAGCACATTAATCTCATGAAGACATCAGACAGCTATG
GGTTCAAAGAGGAATATGAGAGCTTTTTTGAAGGACAGTCAGCATCTTGGGATGTAGCTA
AAAAAGATCAAAATAGAGCAAAAAACCGATATGGAAACATTATAGCATATGATCACTCCA
GAGTGATTTTGCAACCCGTAGAGGATGATCCTTCCTCAGATTATATTAATGCCAACTATA
TTGATGGCTACCAGAGACCAAGTCATTACATTGCAACCCAAGGTCCCGTTCATGAAACAG
TGTATGATTTCTGGAGGATGATTTGGCAAGAACAATCTGCTTGCATTGTGATGGTTACAA
ATTTAGTTGAGGTTGGCCGGGTTAAATGCTATAAATATTGGCCTGATGATACTGAAGTTT
ATGGTGACTTCAAAGTAACGTGTGTAGAAATGGAACCACTTGCTGAATATGTAGTTAGGA
CATTCACCCTGGAAAGGAGGGGGTACAATGAAATCCGTGAAGTTAAACAGTTCCATTTCA
CGGGCTGGCCTGACCATGGAGTGCCCTACCATGCTACAGGGCTGCTTTCCTTTATCCGGC
GAGTCAAGTTATCAAACCCTCCCAGTGCTGGCCCCATCGTTGTACATTGCAGTGCTGGTG
CTGGACGAACTGGCTGCTACATTGTGATTGACATCATGCTAGACATGGCTGAAAGAGAGG
GTGTTGTTGATATTTACAATTGTGTCAAAGCCTTAAGATCTCGGCGTATTAATATGGTCC
AGACAGAGGAACAGTACATTTTTATTCATGATGCCATTTTAGAAGCCTGCTTATGTGGAG
AAACTGCCATACCTGTCTGTGAATTTAAAGCTGCATATTTTGATATGATTAGAATAGACT
CCCAGACTAACTCTTCACATCTCAAGGATGAATTTCAGACTCTGAATTCAGTCACCCCTC
GACTACAAGCTGAAGACTGCAGTATAGCGTGCCTGCCAAGGAACCATGACAAGAACCGTT
TCATGGACATGCTGCCACCTGACAGATGTCTGCCTTTTTTAATTACAATTGATGGGGAGA
GCAGTAACTACATCAATGCTGCTCTTATGGACAGCTACAGGCAACCAGCTGCTTTCATCG
TCACACAATACCCTCTGCCAAACACTGTAAAAGACTTCTGGAGATTAGTGTATGATTATG
GCTGTACCTCCATTGTGATGTTAAACGAAGTCGACTTGTCCCAGGGCTGCCCTCAGTACT
GGCCAGAGGAAGGGATGCTACGATATGGCCCCATCCAAGTGGAATGTATGTCTTGTTCAA
TGGACTGTGATGTGATCAACCGGATTTTTAGGATATGCAATCTAACAAGACCACAGGAAG
GTTATCTGATGGTGCAACAGTTTCAGTACCTAGGATGGGCTTCTCATCGAGAAGTGCCTG
GATCCAAAAGGTCATTCTTGAAACTGATACTTCAGGTGGAAAAGTGGCAGGAGGAATGCG
AGGAAGGGGAAGGCCGGACGATTATCCACTGCCTAAATGGTGGCGGGCGAAGTGGCATGT
TCTGTGCTATAGGCATCGTTGTTGAAATGGTGAAACGGCAAAATGTTGTCGATGTTTTCC
ATGCAGTAAAGACACTGAGGAACAGCAAGCCAAACATGGTGGAAGCCCCGGAGCAATACC
GTTTCTGCTATGATGTAGCTTTGGAGTACCTGGAATCATCTAAGGGCGAATTCGTTTAAA
CCTGCAGGACTAGTCCCTTTAGTGAGGGTTAA
Protein sequence predicted based on nucleotide sequence
RKDAMGNTRQEMTHMVNAMDRSYADQSTLHAEDPLSITFMDQHNFSPRYENHSATAESSR LLDVPRYLCEGTESPYQTGQLHPAIRVADLLQHINLMKTSDSYGFKEEYESFFEGQSASW DVAKKDQNRAKNRYGNIIAYDHSRVILQPVEDDPSSDYINANYIDGYQRPSHYIATQGPV HETVYDFWRMIWQEQSACIVMVTNLVEVGRVKCYKYWPDDTEVYGDFKVTCVEMEPLAEY VVRTFTLERRGYNEIREVKQFHFTGWPDHGVPYHATGLLSFIRRVKLSNPPSAGPIVVHC SAGAGRTGCYIVIDIMLDMAEREGVVDIYNCVKALRSRRINMVQTEEQYIFIHDAILEAC LCGETAIPVCEFKAAYFDMIRIDSQTNSSHLKDEFQTLNSVTPRLQAEDCSIACLPRNHD KNRFMDMLPPDRCLPFLITIDGESSNYINAALMDSYRQPAAFIVTQYPLPNTVKDFWRLV YDYGCTSIVMLNEVDLSQGCPQYWPEEGMLRYGPIQVECMSCSMDCDVINRIFRICNLTR PQEGYLMVQQFQYLGWASHREVPGSKRSFLKLILQVEKWQEECEEGEGRTIIHCLNGGGR SGMFCAIGIVVEMVKRQNVVDVFHAVKTLRNSKPNMVEAPEQYRFCYDVALEYLESS
Coding Sequence Details
Insert Sequence Verified?:
Y
Verification Method:
Sequence Verification
Mutations:
None
Discrepancy:
None
5' Linker Sequence:
None
3' Linker Sequence:
None
Recommended Growth Condition:
Distributed in bacterial strain:
DH5-alpha T1 phage resistant
Antibiotic Selection:
Host Type:
bacterial
Marker:
ampicillin
Host Type: bacterial Marker: kanamycin
Host Type: bacterial Marker: kanamycin
Bacterial Selection Condition:
50 ug/mL ampicillin
Growth Condition:
Growth at 37 degrees C in LB supplemented with ampicillin (50 ug/mL)
Comments:
None
Protein Expression Results:
ProteinExpressed: Not_Applicable
ProteinPurified: Not_Applicable
SolubleProtein: Not_Applicable
Find experimental protocols in NYSGXRC-8618a
Recommended expression in:
Not Applicable
Vector Name:
pCR4zeroBLUNT-TOPO
Synonyms:
pCR-4, pCR-IV, pCR4, pCR4-TOPO
Description:
TOPO cloning vector with M13 forward/reverse, T3, and T7 primers. Ampicillin and kanamycin resistance bacteria.
Comments:
Empty vector is a product available from Invitrogen.
Size (bp):
3956
Parent Vector:
None
Properties:
TOPO Cloning
Author Name:
Invitrogen
Publications:
None
Vector Map:
Vector Sequence:
Sequence:
Vector Features:
Type
Name
Description
Start
End
bacterial origin
pUC ori
pUC bacterial origin of replication
3161
3834
primer site
M13 reverse
M13 reverse priming site
205
221
primer site
T3
T3 priming site
243
262
primer site
M13 forward
M13 forward priming site
355
370
primer site
T7
T7 priming site
328
347
promoter
lac
lac promoter region
2
216
selectable marker
AmpR
Ampicillin resistance gene
2203
3063
selectable marker
KanR
Kanamycin resistance gene
1159
1953
