SAV0913 (S. aureus subsp. aureus Mu50 )
In pSpeedET (bacterial expression vector)

Explanation of Terms

Gene:
Gene Symbol:  SAV0913
Gene Name:  amidase
Original Clone ID:
34239
Keyword:
full-length cds
Species:
Staphylococcus aureus subsp. aureus Mu50
Type:
cDNA
Vector Name:
pSpeedET
Format: 
CLOSED
Source:
Joint Center for Structural Genomics
Description:
None
Comments:
None
Mutation / Discrepancy:
No / No
Publications:
None
Authors:
PSI
Joint Center for Structural Genomics
SaCD00531381
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AVAILABLE
No restriction
Special MTA: PSI
Insert sequence: 1446nts
Open reading frame: 51 to 1496

CTGATAAAATTCATCATCATCATCATCACGAAAACCTGTACTTCCAGGGCATGCAAGCAA
AATTAACTAAAAAAGAGTTTATAGAGTGGTTGAAAACTTCTGAGGGAAAACAATTCAATG
TGGACTTATGGTATGGATTTCAATGCTTTGATTATGCCAATGCTGGTTGGAAAGTTTTGT
TTGGATTACTTCTGAAAGGTTTAGGTGCAAAAGATATACCATTTGCAAACAATTTCGATG
GACTAGCTACTGTATACCAAAATACACCGGACTTTTTGGCACAACCCGGCGACATGGTTG
TATTCGGTAGCAATTACGGTGCAGGATACGGACACGTAGCATGGGTAATTGAAGCAACTT
TAGATTATATCATTGTATATGAGCAGAATTGGCTAGGCGGTGGCTGGACTGACAGAATCG
AACAACCCGGCTGGGGTTGGGAAAAAGTTACAAGACGACAACATGCTTACGATTTCCCTA
TGTGGTTTATCCGTCCTAACTTCAAAAGCGAAACAGCTCCACGATCAATACAATCTCCTA
CGCAAGCATCTAAAAAGGAAACAGCTAAGCCACAACCTAAAGCGGTAGAACTTAAAATTA
TCAAAGATGTGGTTAAAGGTTATGACCTTCCTAAACGTGGTGGTAATCCTAAGGGTATAG
TTATTCATAACGACGCAGGAAGCAAAGGGGCAACAGCAGAAGCGTATCGAAACGGATTAG
TTAACGCACCTTTATCAAGATTAGAAGCGGGTATTGCGCATAGTTATGTATCAGGTAACA
CAGTGTGGCAAGCTTTAGATGAATCGCAAGTAGGTTGGCATACTGCTAACCAATTAGGCA
ATAAATATTATTACGGTATTGAAGTGTGTCAATCAATGGGAGCGGATAATGCGACGTTTT
TAAAAAATGAACAGGCGACTTTCCAAGAATGCGCTAGATTGTTGAAAAAATGGGGATTAC
CAGCAAACAGAAATACAATCAGATTACACAACGAATTCACTTCAACATCATGCCCACACA
GAAGCTCAGTATTGCACACTGGTTTTGACCCAGTAACTCGTGGCCTATTGCCGGAAGATA
AACAATTACAACTTAAAGACTACTTTATCAAGCAAATTAGAGTGTATATGGACGGTAAGA
TACCAGTTGCCACTGTCTCTAATGAGTCAAGCGCTTCAAGTAATACAGTTAAACCAGTTG
CGAGTGCATGGAAACGTAATAAATATGGTACTTACTACATGGAAGAAAGTGCTAGATTCA
CAAACGGTAATCAACCAATCACTGTAAGAAAAATAGGACCATTCTTATCATGCCCGGTAG
CTTACCAATTCCAACCTGGTGGATATTGTGATTATACAGAAGTGATGTTACAAGATGGTC
ATGTTTGGGTAGGATATACATGGGAGGGGCAACGTTATTACTTGCCTATTAGAACATGGA
ATGGTTCTGCCCCACCTAATCAGATATTAGGTGACTTATGGGGAGAAATCAGTTAACGCG
ACTTAATTAATTTGGCGGATGAGAGAAGATTTTC

Protein sequence predicted based on nucleotide sequence

MQAKLTKKEFIEWLKTSEGKQFNVDLWYGFQCFDYANAGWKVLFGLLLKGLGAKDIPFAN
NFDGLATVYQNTPDFLAQPGDMVVFGSNYGAGYGHVAWVIEATLDYIIVYEQNWLGGGWT
DRIEQPGWGWEKVTRRQHAYDFPMWFIRPNFKSETAPRSIQSPTQASKKETAKPQPKAVE
LKIIKDVVKGYDLPKRGGNPKGIVIHNDAGSKGATAEAYRNGLVNAPLSRLEAGIAHSYV
SGNTVWQALDESQVGWHTANQLGNKYYYGIEVCQSMGADNATFLKNEQATFQECARLLKK
WGLPANRNTIRLHNEFTSTSCPHRSSVLHTGFDPVTRGLLPEDKQLQLKDYFIKQIRVYM
DGKIPVATVSNESSASSNTVKPVASAWKRNKYGTYYMEESARFTNGNQPITVRKIGPFLS
CPVAYQFQPGGYCDYTEVMLQDGHVWVGYTWEGQRYYLPIRTWNGSAPPNQILGDLWGEI
S*

Coding Sequence Details

Insert Sequence Verified?:
Y
Verification Method:
Sequence Verification
Mutations:
None
Discrepancy:
None
5' Linker Sequence:
None
3' Linker Sequence:
None

Recommended Growth Condition:

Distributed in bacterial strain:
DH5-alpha T1 phage resistant
Antibiotic Selection:
Host Type:  bacterial    Marker:  kanamycin
Bacterial Selection Condition: 50ug/mL kanamycin
Growth Condition: Growth with the single antibiotic in LB at 37 degrees is recommended.
Comments: Conditions for Gateway-type vectors in recombined (with insert) form and other kanamycin resistant vectors.
Protein Expression Results:
ProteinExpressed: Not_Applicable
ProteinPurified: Protein_Purified
SolubleProtein: Tested_Not_Soluble
Find experimental protocols in 377306
Recommended expression in:
Not Applicable