SAG1362 (S. agalactiae 2603V/R )
In pSpeedET (bacterial expression vector)

Explanation of Terms

Gene:
Gene Symbol:  SAG1362
Gene Name:  carbamoyl-phosphate synthase large subunit
Original Clone ID:
34250
Keyword:
full-length cds
Species:
Streptococcus agalactiae 2603V/R
Type:
cDNA
Vector Name:
pSpeedET
Format: 
CLOSED
Source:
Joint Center for Structural Genomics
Description:
None
Comments:
None
Mutation / Discrepancy:
No / No
Publications:
None
Authors:
PSI
Joint Center for Structural Genomics
SaCD00531384
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AVAILABLE
No restriction
Special MTA: PSI
Insert sequence: 1599nts
Open reading frame: 51 to 1649

CTGATAAAATTCATCATCATCATCATCACGAAAACCTGTACTTCCAGGGCATGCTAAATA
CAAAGGATAAACAGCCTATTTTGCTTATTATTTTCGGACAGAAAATGAAAGAATATGACG
ATTTAATTAGATCAACTGTTGAGAGGTTATCTAAAAGGGATAGACCTGTTAGTATTCTTA
TCTCGAAGGAGCTAAACCATGTGATTCACCATTATGATGCACAATTTGAAAATCTTGAGA
CGCTTGTGGGGTATATAAATACACATGAAGATAGTTTGATTTACCCCCTTTGCTTAGATA
AAAATCATGAGCTATTTCAAGAGTTTTTAGCGTGGCAGAGTCATTATTCAAGGCAGTATA
TCAGCGATAATTTATCAGAAAAAAGTGTTCAGGCAATAGACAATAATCAAGACTTTGTGC
AAGTAGATTGTTTAGTAATGAGAGACTCTTTGAATAATTGTTTATATGTCTCTGATTTAG
AATATATTGAATCTAATAAAACAACTGGTAAGTCTCTAGCGATTGTACCAAGTCAAACTT
TATCCGATGCTGCTAGGCAAACAATTCGAGATGTTGCATTTGATGTGTGTCGAAAAGCAA
ACATTATTGGAGTATGTTACTTTAGTTTCTTAATAGATTTGAACAGTTTGGATTATCATA
TAATTAGTTTATCATCTGGTTTGTCTCATCAAAGCATTTTATTTGAGACTATTACGACCT
ATCCTGTTTTAGAGATAGCAACTAAATTGACAGTGGGATATACTTTTAGTCAACTGAAGC
ATTCTTATTACCCAAATACCTCTGCCTTTTTAGAGCCGCAATTAGACTATGTAGCTACCG
TATCTTTTAGTTTTGAAAAAGTTGACTATATCTTTTTTGCTCGTAATATAGAGCAATTAT
TTTTAAATTTACTCGAGGCTAGCAGTCATGATCATTTTCCATTTCTGAGTGATATTAGCG
AGGAAGATTTGATGTTTGCTTTGATTCAGAAAAAAGAAAATCGTCTAGCTTATCTTTTGG
AGGCTTTTAGACGTGGCTTTGATTTGTATGATTTATCCTCAGTTACTAAAATCAACCCTT
TTTACTTAGATAAATGTTTACATATTGTAGAGTTGTATGAAAACCTGAATAAAAGCCAAT
ATAATGTAGATATTTATAAGGAAGCTAAAAGGTATGGTTTTTCGGATGACTATATTGCTA
GTAGTTGGCAAATCTCTTTGATAGACATGTTAGAATATCGAAAAAAACATAGTGTCGCAC
CTGTTCTGAAGCAAGTCGAACAAAGTTCTGGTGTTCTAACTGGCCATCAAATTCAGTATT
TTCGAAGCTATGATTGGCATTCAGACTACATTTCTAGCGGTTGTCAAAAGGCTTTGATAA
TGGTAGACAAGGGTTATTCCCTAGTAAAATTAAATGAGTTAATTAAGCAAATCAAACAAA
CTCATTTAGAATTATTAATTGTTACCAATCAACCTCTCCTCATAGAACAATTAAATGATA
CATCTATAATTTTTGACACTATAGGTATTGAGACCATATTAACAATTATGGGTATAGAAG
AGGTGGAAGAAGTGTATTTATTGGGAGAAAGTCAAGCAGACATGTTTTCAAAAATGAAAA
ATTTTTCTATCTCTTGTCAGTCTATTTAACGCGACTTAATTAATTTGGCGGATGAGAGAA
GATTTTCA

Protein sequence predicted based on nucleotide sequence

MLNTKDKQPILLIIFGQKMKEYDDLIRSTVERLSKRDRPVSILISKELNHVIHHYDAQFE
NLETLVGYINTHEDSLIYPLCLDKNHELFQEFLAWQSHYSRQYISDNLSEKSVQAIDNNQ
DFVQVDCLVMRDSLNNCLYVSDLEYIESNKTTGKSLAIVPSQTLSDAARQTIRDVAFDVC
RKANIIGVCYFSFLIDLNSLDYHIISLSSGLSHQSILFETITTYPVLEIATKLTVGYTFS
QLKHSYYPNTSAFLEPQLDYVATVSFSFEKVDYIFFARNIEQLFLNLLEASSHDHFPFLS
DISEEDLMFALIQKKENRLAYLLEAFRRGFDLYDLSSVTKINPFYLDKCLHIVELYENLN
KSQYNVDIYKEAKRYGFSDDYIASSWQISLIDMLEYRKKHSVAPVLKQVEQSSGVLTGHQ
IQYFRSYDWHSDYISSGCQKALIMVDKGYSLVKLNELIKQIKQTHLELLIVTNQPLLIEQ
LNDTSIIFDTIGIETILTIMGIEEVEEVYLLGESQADMFSKMKNFSISCQSI*

Coding Sequence Details

Insert Sequence Verified?:
Y
Verification Method:
Sequence Verification
Mutations:
None
Discrepancy:
None
5' Linker Sequence:
None
3' Linker Sequence:
None

Recommended Growth Condition:

Distributed in bacterial strain:
DH5-alpha T1 phage resistant
Antibiotic Selection:
Host Type:  bacterial    Marker:  kanamycin
Bacterial Selection Condition: 50ug/mL kanamycin
Growth Condition: Growth with the single antibiotic in LB at 37 degrees is recommended.
Comments: Conditions for Gateway-type vectors in recombined (with insert) form and other kanamycin resistant vectors.
Protein Expression Results:
ProteinExpressed: Not_Applicable
ProteinPurified: Protein_Purified
SolubleProtein: Tested_Not_Soluble
Find experimental protocols in 377278
Recommended expression in:
Not Applicable