DNASU Plasmid | fixL (X. campestris ) in pSpeedET (bacterial expression vector)

Explanation of Terms

Gene:

Gene Symbol: fixL

Gene Name: histidine kinase

Original Clone ID:

33261

Keyword:

full-length cds

Species:

Xanthomonas campestris

Type:

cDNA

Vector Name:

pSpeedET

Format:

CLOSED

Source:

Joint Center for Structural Genomics

Description:

None

Comments:

None

Mutation / Discrepancy:

No / No

Publications:

None

Authors:

PSI
Joint Center for Structural Genomics

XcCD00531393

Price:

AVAILABLE

No restriction
Special MTA: PSI

Insert Sequence
Protein Sequence

Insert sequence: 1623nts

Open reading frame: 51 to 1673

CTGATAAAATTCATCATCATCATCATCACGAAAACCTGTACTTCCAGGGCATGAACGATT
CCGGTTACGGAGAGCCACGGGCGCCATTGATTGGCGAATCGCAGACGCTTCCGGTCAGCA
AGCATCGCTCGGACATCTTCTTTGCTGCGGTGGAAACCACCCGCATGCCGATGACGGTGA
CCGACCCATACCTGCCGGATAACCCGATCGTGTTCGCCAACCGGGCCTTCCTGGAAATGA
CCGGGTACGCTGCCGACGAAATCATCGGCAACAACTGCCGCTTCCTGCAGGGCCCGGAGA
CCGACCGCCAGGCGGTGGCCGATGTGCGTGAGGCCATCGACAACCGCCGCGAATTCGCCA
CTGAAGTGCTGAATTATCGCAAGGACGGCTCAACATTCTGGAATGCGCTGTTCGTTTCTC
CGGTCTTCGATGACACCGGCAAGCTGGTGTATTTCTTTGGCTCGCAGCTGGACGTCAGCC
GCCGCCGTGATGCCGAAGATGCCCTGCGCCAGGCGCAGAAGATGGAAGCGCTGGGCCAGC
TCACCGGCGGCATTGCACACGACTTCAACAATCTGCTGCAGGTGATGTCCGGGCATCTGG
AATTCATCCAGATGATGGTCAGCAACGGCAACGGCAGCCCCGATCGCATCGCGGTCAGCG
CGGAGCACGCCGCCGCGGCGGCGGCCAAGGCCGCGACCTTGACCCAGCAGTTGCTGGCGT
TTTCGCGTAAACAGAAATTGCGTGGCCGGGTGGTGAATCTCAACGGCCTGGTGTCGGGCA
TGAACAATATGGCCGAGCGCGCGCTGGGGGGCGGCATCACATTGCGTCAGGCGCTGGAAG
AACGGCTGTGGAATTGCCAGATCGACACGACGCAGGCGGAAGTGGCGCTGTTGAATGTGC
TGATCAATGCGCGTGACGCCATGGCCAATGCCGAGCGCAAGGAAGTGATGGTGCAGACGC
AGAATGTGGAGATCACCAGCCACGACCTGTCGATGTATCACCAGCTCGCGCCGGGCCGCT
ACGTGAGTATTGCCGTGACCGACAGCGGCTCGGGCATGCCGCCGGAAGTGGTGAGCCGGG
TCATGGAGCCGTTCTTCACCACCAAGGAAGAGGGGCAGGGCACGGGCCTGGGCTTGTCGA
TGGTGTATGGCTTCGTCAAGCAATCCGGTGGCACCGTGCGCATCTACAGCGAAGTGGGCG
AAGGCACCACGGTGCGGCTGTATTTCCCCGCCTCCAGCGATTACGAAAACGCGCTGCCGT
CCACCAAGAACCGCGCCTTGGACAAGGGCGGCAACGAAACCATCCTGATCGTGGAAGACA
AGGAGGACGTGGCGGTGGTGGCCAAGATGTTCCTGGAAGGTGCCGGTTATCGCGCGCTGT
CCGCGTCGAGCGGGCGCGAAGCGATCGAGGTGCTGGAGCAGCATCCGGAAGTGGACGCCT
TGTTCACCGACCTGATCATGCCAGGCGGCATGAACGGCGTGGTGCTGGCGCGCGAAGCAC
GGCGGATGCTGCCCAAGATCAAGGTGCTGCTGACCACCGGCTATGCCGATGCCAGCATCC
AGCGCACCGATGTGGGCGGCGCGGAGTTCGACGTGGTCAACAAGCCGTACACGCAGAAGG
AATTGCTCAAGCGCATTCGCATGTTGCTGGATGGTCCCACCGGCGTTGGTTAACGCGACT
TAATTAATTTGGCGGATGAGAGAAGATTTTC

Protein sequence predicted based on nucleotide sequence

MNDSGYGEPRAPLIGESQTLPVSKHRSDIFFAAVETTRMPMTVTDPYLPDNPIVFANRAF
LEMTGYAADEIIGNNCRFLQGPETDRQAVADVREAIDNRREFATEVLNYRKDGSTFWNAL
FVSPVFDDTGKLVYFFGSQLDVSRRRDAEDALRQAQKMEALGQLTGGIAHDFNNLLQVMS
GHLEFIQMMVSNGNGSPDRIAVSAEHAAAAAAKAATLTQQLLAFSRKQKLRGRVVNLNGL
VSGMNNMAERALGGGITLRQALEERLWNCQIDTTQAEVALLNVLINARDAMANAERKEVM
VQTQNVEITSHDLSMYHQLAPGRYVSIAVTDSGSGMPPEVVSRVMEPFFTTKEEGQGTGL
GLSMVYGFVKQSGGTVRIYSEVGEGTTVRLYFPASSDYENALPSTKNRALDKGGNETILI
VEDKEDVAVVAKMFLEGAGYRALSASSGREAIEVLEQHPEVDALFTDLIMPGGMNGVVLA
REARRMLPKIKVLLTTGYADASIQRTDVGGAEFDVVNKPYTQKELLKRIRMLLDGPTGVG
*

Coding Sequence Details

Insert Sequence Verified?:

Verification Method:

Sequence Verification

Mutations:

None

Discrepancy:

None

5' Linker Sequence:

None

3' Linker Sequence:

None

Recommended Growth Condition:

Distributed in bacterial strain:

DH5-alpha T1 phage resistant

Antibiotic Selection:

Host Type: bacterial Marker: kanamycin

Bacterial Selection Condition: 50ug/mL kanamycin

Growth Condition: Growth with the single antibiotic in LB at 37 degrees is recommended.

Comments: Conditions for Gateway-type vectors in recombined (with insert) form and other kanamycin resistant vectors.

Protein Expression Results:

ProteinExpressed: Not_Applicable

ProteinPurified: Protein_Purified

SolubleProtein: Tested_Not_Soluble

Find experimental protocols in 375324

Recommended expression in:

Not Applicable

Vector Name:

pSpeedET

Synonyms:

pSpeed-ET

Sequencing Primer:

Forward: pBAD forward

Reverse: alt_bad_rev

Description:

Bacterial expression vector, arabinose or T7 induced expression, adds N-terminal MGSDKIHHHHHHENLYFQG tag; kanamycin resistance; PIPE cloning.

Comments:

If this vector contains an insert, the ccdB death cassette will not be present.

Size (bp):

5464

Parent Vector:

None

Properties:

PIPE cloning, bacterial expression, in vitro transcription, inducible expression, with tag/fusion/marker

Author Name:

Joint Center for Structural Genomics

Publications:

PMID: 18004753

Title: Combining the polymerase incomplete primer extension method for cloning and mutagenesis with microscreening to accelerate structural genomics efforts.

Vector Map:

Vector Sequence:

Sequence:

AAGAAACCAATTGTCCATATTGCATCAGACATTGCCGTCACTGCGTCTTTTACTGGCTCTTCTCGCTAACCAAACCGGTAACCCCGCTTATTAAAAGCATTCTGTAACAAAGCGGGACCAAAGCCATGACAAAAACGCGTAACAAAAGTGTCTATAATCACGGCAGAAAAGTCCACATTGATTATTTGCACGGCGTCACACTTTGCTATGCCATAGCATTTTTATCCATAAGATTAGCGGATCCTACCTGACGCTTTTTATCGCAACTCTCTACTGTTTCTCCATACCCGTAATACGACTCACTATAGGGTTTTGTTTAACTTTAAGAAGGAGATATACATACCCATGGGATCTGATAAAATTCATCATCATCATCATCACGAAAACCTGTACTTCCAGGGCGCCGGCACCCCAGGCTTTACACTTTATGCTTCCGGCTCGTATAATGTGTGGATTTTGAGTTAGGATCCGGCGAGATTTTCAGGAGCTAAGGAAGCTAAAATGGAGAAAAAAATCACTGGATATACCACCGTTGATATATCCCAATGGCATCGTAAAGAACATTTTGAGGCATTTCAGTCAGTTGCTCAATGTACCTATAACCAGACCGTTCAGCTGGATATTACGGCCTTTTTAAAGACCGTAAAGAAAAATAAGCACAAGTTTTATCCGGCCTTTATTCACATTCTTGCCCGCCTGATGAATGCTCATCCGGAATTCCGTATGGCAATGAAAGACGGTGAGCTGGTGATATGGGATAGTGTTCACCCTTGTTACACCGTTTTCCATGAGCAAACTGAAACGTTTTCATCGCTCTGGAGTGAATACCACGACGATTTCCGGCAGTTTCTACACATATATTCGCAAGATGTGGCGTGTTACGGTGAAAACCTGGCCTATTTCCCTAAAGGGTTTATTGAGAATATGTTTTTCGTCTCAGCCAATCCCTGGGTGAGTTTCACCAGTTTTGATTTAAACGTGGCCAATATGGACAACTTCTTCGCCCCCGTTTTCACCATGGGCAAATATTATACGCAAGGCGACAAGGTGCTGATGCCGCTGGCGATTCAGGTTCATCATGCCGTTTGTGATGGCTTCCATGTCGGCAGAATGCTTAATGAATTACAACAGTACTGCGATGAGTGGCAGGGCGGGGCGTAAACGCGTGGATCCGGCTTACTAAAAGCCAGATAACAGTATGCGTATTTGCGCGCTGATTTTTGCGGTATAAGAATATATACTGATATGTATACCCGAAGTATGTCAAAAAGAGGTGTGCTATGAAGCAGCGTATTACAGTGACAGTTGACAGCGACAGCTATCAGTTGCTCAAGGCATATATGATGTCAATATCTCCGGTCTGGTAAGCACAACCATGCAGAATGAAGCCCGTCGTCTGCGTGCCGAACGCTGGAAAGCGGAAAATCAGGAAGGGATGGCTGAGGTCGCCCGGTTTATTGAAATGAACGGCTCTTTTGCTGACGAGAACAGGGGCTGGTGAAATGCAGTTTAAGGTTTACACCTATAAAAGAGAGAGCCGTTATCGTCTGTTTGTGGATGTACAGAGTGATATTATTGACACGCCCGGGCGACGGATGGTGATCCCCCTGGCCAGTGCACGTCTGCTGTCAGATAAAGTCTCCCGTGAACTTTACCCGGTGGTGCATATCGGGGATGAAAGCTGGCGCATGATGACCACCGATATGGCCAGTGTGCCGGTCTCCGTTATCGGGGAAGAAGTGGCTGATCTCAGCCACCGCGAAAATGACATCAAAAACGCCATTAACCTGATGTTCTGGGGAATATAACTTAATTAACTCTAGATAACTGAGAAGGAGATATACATACCCGGGTAAGTTTAAACGGTCTCCAGCTTGGCTGTTTTGGCGGATGAGAGAAGATTTTCAGCCTGATACAGATTAAATCAGAACGCAGAAGCGGTCTGATAAAACAGAATTTGCCTGGCGGCAGTAGCGCGGTGGTCCCACCTGACCCCATGCCGAACTCAGAAGTGAAACGCCGTAGCGCCGATGGTAGTGTGGGGTCTCCCCATGCGAGAGTAGGGAACTGCCAGGCATCAAATAAAACGAAAGGCTCAGTCGAAAGACTGGGCCTTTCGTTTTATCTGTTGTTTGTCGGTGAACGCTCTCCTGAGTAGGACAAATCCGCCGGGAGCGGATTTGAACGTTGCGAAGCAACGGCCCGGAGGGTGGCGGGCAGGACGCCCGCCATAAACTGCCAGGCATCAAATTAAGCAGAAGGCCATCCTGACGGATGGCCTTTTTGCGTTTCTACAAACTCTTTTTGTTTATTTTTCTAAATACATTCAAATATGTATCCGCTCATGAGACAATAACCCTGATAAATGCTTCAATAATATTGAAAAAGGAAGAGTATGAGCCATATTCAACGGGAAACGTCTTGCTCTAGGCCGCGATTAAATTCCAACATGGATGCTGATTTATATGGGTATAAATGGGCTCGCGATAATGTCGGGCAATCAGGTGCGACAATCTATCGATTGTATGGGAAGCCCGATGCGCCAGAGTTGTTTCTGAAACATGGCAAAGGTAGCGTTGCCAATGATGTTACAGATGAGATGGTCAGACTAAACTGGCTGACGGAATTTATGCCTCTTCCGACCATCAAGCATTTTATCCGTACTCCTGATGATGCATGGTTACTCACCACTGCGATCCCCGGGAAAACAGCATTCCAGGTATTAGAAGAATATCCTGATTCAGGTGAAAATATTGTTGATGCGCTGGCAGTGTTCCTGCGCCGGTTGCATTCGATTCCTGTTTGTAATTGTCCTTTTAACAGCGATCGCGTATTTCGTCTCGCTCAGGCGCAATCACGAATGAATAACGGTTTGGTTGATGCGAGTGATTTTGATGACGAGCGTAATGGCTGGCCTGTTGAACAAGTCTGGAAAGAAATGCATAAACTTTTGCCATTCTCACCGGATTCAGTCGTCACTCATGGTGATTTCTCACTTGATAACCTTATTTTTGACGAGGGGAAATTAATAGGTTGTATTGATGTTGGACGAGTCGGAATCGCAGACCGATACCAGGATCTTGCCATCCTATGGAACTGCCTCGGTGAGTTTTCTCCTTCATTACAGAAACGGCTTTTTCAAAAATATGGTATTGATAATCCTGATATGAATAAATTGCAGTTTCATTTGATGCTCGATGAGTTTTTCTAACTGTCAGACCAAGTTTACTCATATATACTTTAGATTGATTTAAAACTTCATTTTTAATTTAAAAGGATCTAGGTGAAGATCCTTTTTGATAATCTCATGACCAAAATCCCTTAACGTGAGTTTTCGTTCCACTGAGCGTCAGACCCCGTAGAAAAGATCAAAGGATCTTCTTGAGATCCTTTTTTTCTGCGCGTAATCTGCTGCTTGCAAACAAAAAAACCACCGCTACCAGCGGTGGTTTGTTTGCCGGATCAAGAGCTACCAACTCTTTTTCCGAAGGTAACTGGCTTCAGCAGAGCGCAGATACCAAATACTGTCCTTCTAGTGTAGCCGTAGTTAGGCCACCACTTCAAGAACTCTGTAGCACCGCCTACATACCTCGCTCTGCTAATCCTGTTACCAGTGGCTGCTGCCAGTGGCGATAAGTCGTGTCTTACCGGGTTGGACTCAAGACGATAGTTACCGGATAAGGCGCAGCGGTCGGGCTGAACGGGGGGTTCGTGCACACAGCCCAGCTTGGAGCGAACGACCTACACCGAACTGAGATACCTACAGCGTGAGCTATGAGAAAGCGCCACGCTTCCCGAAGGGAGAAAGGCGGACAGGTATCCGGTAAGCGGCAGGGTCGGAACAGGAGAGCGCACGAGGGAGCTTCCAGGGGGAAACGCCTGGTATCTTTATAGTCCTGTCGGGTTTCGCCACCTCTGACTTGAGCGTCGATTTTTGTGATGCTCGTCAGGGGGGCGGAGCCTATGGAAAAACGCCAGCAACGCGGCCTTTTTACGGTTCCTGGCCTTTTGCTGGCCTTTTGCTCACATGTTCTTTCCTGCGTTATCCCCTGATTCTGTGGATAACCGTATTACCGCCTTTGAGTGAGCTGATACCGCTCGCCGCAGCCGAACGACCGAGCGCAGCGAGTCAGTGAGCGAGGAAGCGGAAGAGCGCCTGATGCGGTATTTTCTCCTTACGCATCTGTGCGGTATTTCACACCGCATATGGTGCACTCTCAGTACAATCTGCTCTGATGCCGCATAGTTAAGCCAGTATACACTCCGCTATCGCTACGTGACTGGGTCATGGCTGCGCCCCGACACCCGCCAACACCCGCTGACGCGCCCTGACGGGCTTGTCTGCTCCCGGCATCCGCTTACAGACAAGCTGTGACCGTCTCCGGGAGCTGCATGTGTCAGAGGTTTTCACCGTCATCACCGAAACGCGCGAGGCAGCAGATCAATTCGCGCGCGAAGGCGAAGCGGCATGCATAATGTGCCTGTCAAATGGACGAAGCAGGGATTCTGCAAACCCTATGCTACTCCGTCAAGCCGTCAATTGTCTGATTCGTTACCAATTATGACAACTTGACGGCTACATCATTCACTTTTTCTTCACAACCGGCACGGAACTCGCTCGGGCTGGCCCCGGTGCATTTTTTAAATACCCGCGAGAAATAGAGTTGATCGTCAAAACCAACATTGCGACCGACGGTGGCGATAGGCATCCGGGTGGTGCTCAAAAGCAGCTTCGCCTGGCTGATACGTTGGTCCTCGCGCCAGCTTAAGACGCTAATCCCTAACTGCTGGCGGAAAAGATGTGACAGACGCGACGGCGACAAGCAAACATGCTGTGCGACGCTGGCGATATCAAAATTGCTGTCTGCCAGGTGATCGCTGATGTACTGACAAGCCTCGCGTACCCGATTATCCATCGGTGGATGGAGCGACTCGTTAATCGCTTCCATGCGCCGCAGTAACAATTGCTCAAGCAGATTTATCGCCAGCAGCTCCGAATAGCGCCCTTCCCCTTGCCCGGCGTTAATGATTTGCCCAAACAGGTCGCTGAAATGCGGCTGGTGCGCTTCATCCGGGCGAAAGAACCCCGTATTGGCAAATATTGACGGCCAGTTAAGCCATTCATGCCAGTAGGCGCGCGGACGAAAGTAAACCCACTGGTGATACCATTCGCGAGCCTCCGGATGACGACCGTAGTGATGAATCTCTCCTGGCGGGAACAGCAAAATATCACCCGGTCGGCAAACAAATTCTCGTCCCTGATTTTTCACCACCCCCTGACCGCGAATGGTGAGATTGAGAATATAACCTTTCATTCCCAGCGGTCGGTCGATAAAAAAATCGAGATAACCGTTGGCCTCAATCGGCGTTAAACCCGCCACCAGATGGGCATTAAACGAGTATCCCGGCAGCAGGGGATCATTTTGCGCTTCAGCCATACTTTTCATACTCCCGCCATTCAGAG

Vector Features:

Type

Name

Description

Start

End

bacterial origin

ori

ColE1-type origin of replication

3306

3988

inducible trxn activator protein gene

araC

araC coding sequence

4563

5438

negative selection marker

ccdB

ccdB death cassette gene (lost in with-insert form)

1596

1805

promoter

T7 promoter

290

310

promoter

araC

araC promoter

256

285

protease cleavage site

TEV

TEV protease cleavage site

382

402

selectable marker

CmR

chloramphenicol resistance gene (CmR) (lost in with-insert form)

502

1158

selectable marker

KanR

kanamycin resistance gene

2396

3208

tag

His

N-terminal 6xHis tag

364

381

trxn regulatory element

arabinose O1 operator

161

182

trxn regulatory element

arabinose O2 operator

trxn termination sequence

rrnB

rrnB T2 transcription terminator

2258

2284

Gene

Gene Symbol :

fixL

GENEID :

998951

Locus Tag :

XCC2421

GI :

21231858

GenBank Accession :

NP_637775

Target GenBank:

None

Protein

SMART domain :

HisKA : His Kinase A (phosphoacceptor) domain

SMART domain :

PAC : Motif C-terminal to PAS motifs (likely to contribute to PAS structural domain)

SMART domain :

PAS : PAS domain

SMART domain :

REC : cheY-homologous receiver domain

SMART domain :

HATPase_c : Histidine kinase-like ATPases

Structural Annotation Wiki :

375324

Clone

Cloning Information :

458195

HIP Master Clone ID :

387681

Original Clone ID :

33261

Experimental

TargetTrack - Experimental data :

375324

Organism

Species Specific ID:

998951

Reagents

Labome :

fixL-cDNA