vacB (P. gingivalis )
In pSGX3 (His-tagged bacterial expression vector)

Explanation of Terms

Gene:
Gene Symbol:  vacB
Gene Name:  ribonuclease R
Original Clone ID:
6169b1BCt1b1
Keyword:
full-length cds
Species:
Porphyromonas gingivalis
Type:
cDNA
Vector Name:
pSGX3
Format: 
CLOSED
Source:
New York SGX Research Center for Structural Genomics
Description:
None
Comments:
Linker not verified.
Mutation / Discrepancy:
No / Yes
Publications:
None
Authors:
PSI
New York SGX Research Center for Structural Genomics
PgCD00531436
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AVAILABLE
No restriction
Special MTA: PSI
Insert sequence: 2130nts
Open reading frame: 51 to 2180

CTAGAAATAATTTTGTTTAACTTTAAGAAGGAGATATACATATGTCCCTTACCAAGGAGG
AGATGCGCAATGCCATTTTGGACATATTCCAAAGCAATCCTCAGAGCGTGATGAACTACA
AGCAGGTAAGCCATCTGATGGGAGTAGAAGCTACACCACAAAAGCTAATGGTGAACTCCC
TGATGGAAGACATGGCTGTGGATGACATCCTGCATGAGATAGAGCGCGGACGCTATCGGT
ACAATGCCTTGGGATTGATTGTCATAGGCACTTTTGAGCGTCGGTTCAATGGCCGCAATT
CGTTTATTCCCGAAGGGGGCGGAACGCCCATTTTCATTGCCGAAAGAAATTCGGCACATG
CCATGGACGGTGACAAAGTGAAAGTACAACTCTTTGCCAAACGTCGTGGTCGCGCGCCGG
AAGCCGCCGTGATCGAGATCATAGAACAGAAGCAGGCCACTTTCGTCGGCAAGATCCAAA
TCAATAGAGACTTTGCTTTCCTTGTTACGGAAGATCGCACCTTAGCGAATGATATTTTCA
TCCCCAAGGACAACCTCGGCAACGCCAAAAACGGCGATAAGGTAATAGTCCGAATACTCG
AATGGCCGGAGCAATCCAAGAATCCCATCGGAGAGGTACAAGCCGTATTGGGACCGGCCG
GAGACAACGATGCCGAGATGAATGCCATTTTGGCCGAGTTCGGATTGCCTTATGTCTACC
CCCAAGAGGTGGAAGAGGCAGCCGACCGTTTGGATGGGAAGATCACAGAAGAAGAGCTTG
CCCGAAGAGAAGATTTTCGGTCGGTACTCACCTTCACCATTGACCCGAAGGATGCCAAGG
ACTTCGATGATGCCATATCGTTCCGTCCGCTGGAGGATGATTGCTATGAGGTAGGCGTAC
ATATCGCCGATGTCTCGCACTACGTAACCGAGGGCGGGATCATAGATCAGGAAGCATACA
ACCGTGCCACAAGTATCTATCTCGTGGATCGCACCATCCCGATGCTCCCCGAACGCCTCT
GCAACGACCTTTGTTCCCTCCGCCCCGACGAAGAGAAATATGCCTATAGCTGTATATTCC
GGATGAATGACGAAGCCAAAGTATTGGATTATCGCATCTGCCACTCCGTGATCAAGAGCG
ACCGCCGGTTCGCCTACGAAGAGGCACAAGCCGTGATCGAGACCGGCGAAGGCGACTGCA
AGGAGGCCATTCTCAAACTCAACGAGCTGGCACAGCGACTTCGCGAAGAACGATTCCAAA
ACGGAGCGATTGCTTTCGAAAGGAAAGAAGTCCGCTTCGAAATAGATGAGAAAGGTCGTC
CTCTGGGTGTAATCGTGAAGGAGAGCAAGGAAGCCAACAAGCTGATCGAAGAGTTCATGC
TTTTGGCCAATAGGACGGTAGCCCGACATATAGGAGAAGAGACCCAAGGGAAACGAGCCA
AGACCTTCGTTTATCGTATCCACGACCTGCCCGATCCCGACAAATTGGAGACCTTGTCGG
ACTTCATCCGCCGATTCGGCTACAAGTTGCGCACCACAGGTAGTAATATGGAGGTAAGCA
AGAGCATCAATTCGCTTCTGGACAACATCCAAAACAAACCGGAAGAAAATTTGATCTCTA
CCGTTGCCATACGCTCCATGGCTAAGGCCATTTACTCGACGGACAACATCGGCCACTACG
GATTGGCATTCGATTTCTACACCCACTTCACTTCGCCCATTCGTCGCTATCCGGACTTGA
TGGTGCACCGATTGCTGACGAAATACCTTTCGGGTGGAAGTTCCGTAGACAAAAATGACT
ATGAGGAAAAGTGCAAGCATTCATCTGCAATGGAGCAATTGGCAGCTTCGGCCGAACGCG
CTTCCATCAAATACAAACAGGTGGAGTTTATGTCCGAACACCTCGGCAAAGTATTCGATG
GTGTAATATCGGGCGTCACCGAATGGGGATTGTACGTAGAGCTGAAGGAAAATATGTGCG
AAGGTCTCATACCCATCCGCTGTCTCGACGACGACTACTACGAGTACGACGACAAGAACT
TCTGCCTCATCGGGCGTCGCTATCGTCACCGCTACAGTCTGGGCGATGCCATCACCGTAC
GTGTTGCCCAAGCCAATCTGGACAGAAAGCAACTCGACTTCGAACCCGTAAGCGAAGGGC
ATCATCACCATCACCACTGAT

Protein sequence predicted based on nucleotide sequence

TKEEMRNAILDIFQSNPQSVMNYKQVSHLMGVEATPQKLMVNSLMEDMAVDDILHEIERG
RYRYNALGLIVIGTFERRFNGRNSFIPEGGGTPIFIAERNSAHAMDGDKVKVQLFAKRRG
RAPEAAVIEIIEQKQATFVGKIQINRDFAFLVTEDRTLANDIFIPKDNLGNAKNGDKVIV
RILEWPEQSKNPIGEVQAVLGPAGDNDAEMNAILAEFGLPYVYPQEVEEAADRLDGKITE
EELARREDFRSVLTFTIDPKDAKDFDDAISFRPLEDDCYEVGVHIADVSHYVTEGGIIDQ
EAYNRATSIYLVDRTIPMLPERLCNDLCSLRPDEEKYAYSCIFRMNDEAKVLDYRICHSV
IKSDRRFAYEEAQAVIETGEGDCKEAILKLNELAQRLREERFQNGAIAFERKEVRFEIDE
KGRPLGVIVKESKEANKLIEEFMLLANRTVARHIGEETQGKRAKTFVYRIHDLPDPDKLE
TLSDFIRRFGYKLRTTGSNMEVSKSINSLLDNIQNKPEENLISTVAIRSMAKAIYSTDNI
GHYGLAFDFYTHFTSPIRRYPDLMVHRLLTKYLSGGSSVDKNDYEEKCKHSSAMEQLAAS
AERASIKYKQVEFMSEHLGKVFDGVISGVTEWGLYVELKENMCEGLIPIRCLDDDYYEYD
DKNFCLIGRRYRHRYSLGDAITVRVAQANLDRKQLDFEPVSEGHHHHHH*

Coding Sequence Details

Insert Sequence Verified?:
Y
Verification Method:
Sequence Verification
Mutations:
None
Discrepancy:
g103a,A35T;a648g,E216E;
t669c,Y223Y;a754g,I252V;

c771t,I257I;g843a,V281V;
a1005g,E335E;c1023t,C341C;

t1104c,A368A;a1195g,K399E;
a1233g,R411R;t1242c,V414V;

a1314g,L438L;c1333t,L445L;
c1365a,G455G;t1470c,G490G;

g1498a,V500M;g1548a,K516K
                                                    

5' Linker Sequence:
None
3' Linker Sequence:
None

Recommended Growth Condition:

Distributed in bacterial strain:
DH5-alpha T1 phage resistant
Antibiotic Selection:
Host Type:  bacterial    Marker:  kanamycin
Bacterial Selection Condition: 50ug/mL kanamycin
Growth Condition: Growth with the single antibiotic in LB at 37 degrees is recommended.
Comments: Conditions for Gateway-type vectors in recombined (with insert) form and other kanamycin resistant vectors.
Protein Expression Results:
ProteinExpressed: Protein_Confirmed
ProteinPurified: Protein_Purified
SolubleProtein: Protein_Soluble
Find experimental protocols in NYSGXRC-6169b
Recommended expression in:
Not Applicable