DNASU Plasmid | LOC114971 (H. sapiens )<br>In pCR4zeroBLUNT-TOPO (TOPO cloning vector)

Explanation of Terms

Gene:

Gene Symbol: PTPMT1

Gene Name: protein tyrosine phosphatase, mitochondrial 1

Original Clone ID:

8742a1ZBt10

Keyword:

full-length cds

Species:

Homo sapiens

Type:

cDNA

Vector Name:

pCR4zeroBLUNT-TOPO

Format:

CLOSED

Source:

New York SGX Research Center for Structural Genomics

Description:

None

Comments:

None

Mutation / Discrepancy:

No / No

Publications:

None

Authors:

PSI
New York SGX Research Center for Structural Genomics

HsCD00531500

Price:

AVAILABLE

No restriction
Special MTA: PSI

Insert Sequence
Protein Sequence

Insert sequence: 600nts

Open reading frame: 1 to 600

Reference Sequence Alignment

GCGGCCACCGCGCTGCTGGAGGCCGGCCTGGCGCGGGTGCTCTTCTACCCGACGCTGCTC
TACACCCTGTTCCGCGGGAAGGTGCCGGGTCGGGCGCACCGGGACTGGTACCACCGCATC
GACCCCACCGTGCTGCTGGGCGCGCTGCCGTTGCGGAGCTTGACGCGCCAGCTGGTACAG
GACGAGAACGTGCGCGGGGTGATCACCATGAACGAGGAGTACGAGACGAGGTTCCTGTGC
AACTCTTCACAGGAGTGGAAGAGACTAGGAGTCGAGCAGCTGCGGCTCAGCACAGTAGAC
ATGACTGGGATCCCCACCTTGGACAACCTCCAGAAGGGAGTCCAATTTGCTCTCAAGTAC
CAGTCGCTGGGCCAGTGTGTTTACGTGCATTGTAAGGCTGGGCGCTCCAGGAGTGCCACT
ATGGTGGCAGCATACCTGATTCAGGTGCACAAATGGAGTCCAGAGGAGGCTGTAAGAGCC
ATCGCCAAGATCCGGTCATACATCCACATCAGGCCTGGCCAGCTGGATGTTCTTAAAGAG
TTCCACAAGCAGATTACTGCACGGGCAACAAAGGATGGGACTTTTGTCATTTCAAAGACA

Protein sequence predicted based on nucleotide sequence

AATALLEAGLARVLFYPTLLYTLFRGKVPGRAHRDWYHRIDPTVLLGALPLRSLTRQLVQ
DENVRGVITMNEEYETRFLCNSSQEWKRLGVEQLRLSTVDMTGIPTLDNLQKGVQFALKY
QSLGQCVYVHCKAGRSRSATMVAAYLIQVHKWSPEEAVRAIAKIRSYIHIRPGQLDVLKE
FHKQITARATKDGTFVISKT

Coding Sequence Details

Insert Sequence Verified?:

Verification Method:

Sequence Verification

Mutations:

None

Discrepancy:

None

5' Linker Sequence:

None

3' Linker Sequence:

None

Recommended Growth Condition:

Distributed in bacterial strain:

DH5-alpha T1 phage resistant

Antibiotic Selection:

Host Type: bacterial Marker: ampicillin
Host Type: bacterial Marker: kanamycin

Bacterial Selection Condition: 50 ug/mL ampicillin

Growth Condition: Growth at 37 degrees C in LB supplemented with ampicillin (50 ug/mL)

Comments: None

Protein Expression Results:

ProteinExpressed: Not_Applicable

ProteinPurified: Not_Applicable

SolubleProtein: Not_Applicable

Find experimental protocols in NYSGXRC-8742a

Recommended expression in:

Not Applicable

Vector Name:

pCR4zeroBLUNT-TOPO

Synonyms:

pCR-4, pCR-IV, pCR4, pCR4-TOPO

Sequencing Primer:

Forward: M13F

Reverse: M13R

Description:

TOPO cloning vector with M13 forward/reverse, T3, and T7 primers. Ampicillin and kanamycin resistance bacteria.

Comments:

Empty vector is a product available from Invitrogen.

Size (bp):

3956

Parent Vector:

None

Properties:

TOPO Cloning

Author Name:

Invitrogen

Publications:

None

Vector Map:

Vector Sequence:

Sequence:

AGCGCCCAATACGCAAACCGCCTCTCCCCGCGCGTTGGCCGATTCATTAATGCAGCTGGCACGACAGGTTTCCCGACTGGAAAGCGGGCAGTGAGCGCAACGCAATTAATGTGAGTTAGCTCACTCATTAGGCACCCCAGGCTTTACACTTTATGCTTCCGGCTCGTATGTTGTGTGGAATTGTGAGCGGATAACAATTTCACACAGGAAACAGCTATGACCATGATTACGCCAAGCTCAGAATTAACCCTCACTAAAGGGACTAGTCCTGCAGGTTTAAACGAATTCGCCCTTAAGGGCGAATTCGCGGCCGCTAAATTCAATTCGCCCTATAGTGAGTCGTATTACAATTCACTGGCCGTCGTTTTACAACGTCGTGACTGGGAAAACCCTGGCGTTACCCAACTTAATCGCCTTGCAGCACATCCCCCTTTCGCCAGCTGGCGTAATAGCGAAGAGGCCCGCACCGATCGCCCTTCCCAACAGTTGCGCAGCCTATACGTACGGCAGTTTAAGGTTTACACCTATAAAAGAGAGAGCCGTTATCGTCTGTTTGTGGATGTACAGAGTGATATTATTGACACGCCGGGGCGACGGATGGTGATCCCCCTGGCCAGTGCACGTCTGCTGTCAGATAAAGTCTCCCGTGAACTTTACCCGGTGGTGCATATCGGGGATGAAAGCTGGCGCATGATGACCACCGATATGGCCAGTGTGCCGGTCTCCGTTATCGGGGAAGAAGTGGCTGATCTCAGCCACCGCGAAAATGACATCAAAAACGCCATTAACCTGATGTTCTGGGGAATATAAATGTCAGGCATGAGATTATCAAAAAGGATCTTCACCTAGATCCTTTTCACGTAGAAAGCCAGTCCGCAGAAACGGTGCTGACCCCGGATGAATGTCAGCTACTGGGCTATCTGGACAAGGGAAAACGCAAGCGCAAAGAGAAAGCAGGTAGCTTGCAGTGGGCTTACATGGCGATAGCTAGACTGGGCGGTTTTATGGACAGCAAGCGAACCGGAATTGCCAGCTGGGGCGCCCTCTGGTAAGGTTGGGAAGCCCTGCAAAGTAAACTGGATGGCTTTCTTGCCGCCAAGGATCTGATGGCGCAGGGGATCAAGCTCTGATCAAGAGACAGGATGAGGATCGTTTCGCATGATTGAACAAGATGGATTGCACGCAGGTTCTCCGGCCGCTTGGGTGGAGAGGCTATTCGGCTATGACTGGGCACAACAGACAATCGGCTGCTCTGATGCCGCCGTGTTCCGGCTGTCAGCGCAGGGGCGCCCGGTTCTTTTTGTCAAGACCGACCTGTCCGGTGCCCTGAATGAACTGCAAGACGAGGCAGCGCGGCTATCGTGGCTGGCCACGACGGGCGTTCCTTGCGCAGCTGTGCTCGACGTTGTCACTGAAGCGGGAAGGGACTGGCTGCTATTGGGCGAAGTGCCGGGGCAGGATCTCCTGTCATCTCACCTTGCTCCTGCCGAGAAAGTATCCATCATGGCTGATGCAATGCGGCGGCTGCATACGCTTGATCCGGCTACCTGCCCATTCGACCACCAAGCGAAACATCGCATCGAGCGAGCACGTACTCGGATGGAAGCCGGTCTTGTCGATCAGGATGATCTGGACGAAGAGCATCAGGGGCTCGCGCCAGCCGAACTGTTCGCCAGGCTCAAGGCGAGCATGCCCGACGGCGAGGATCTCGTCGTGACCCATGGCGATGCCTGCTTGCCGAATATCATGGTGGAAAATGGCCGCTTTTCTGGATTCATCGACTGTGGCCGGCTGGGTGTGGCGGACCGCTATCAGGACATAGCGTTGGCTACCCGTGATATTGCTGAAGAGCTTGGCGGCGAATGGGCTGACCGCTTCCTCGTGCTTTACGGTATCGCCGCTCCCGATTCGCAGCGCATCGCCTTCTATCGCCTTCTTGACGAGTTCTTCTGAATTATTAACGCTTACAATTTCCTGATGCGGTATTTTCTCCTTACGCATCTGTGCGGTATTTCACACCGCATCAGGTGGCACTTTTCGGGGAAATGTGCGCGGAACCCCTATTTGTTTATTTTTCTAAATACATTCAAATATGTATCCGCTCATGAGATTATCAAAAAGGATCTTCACCTAGATCCTTTTAAATTAAAAATGAAGTTTTAAATCAATCTAAAGTATATATGAGTAAACTTGGTCTGACAGTTACCAATGCTTAATCAGTGAGGCACCTATCTCAGCGATCTGTCTATTTCGTTCATCCATAGTTGCCTGACTCCCCGTCGTGTAGATAACTACGATACGGGAGGGCTTACCATCTGGCCCCAGTGCTGCAATGATACCGCGAGACCCACGCTCACCGGCTCCAGATTTATCAGCAATAAACCAGCCAGCCGGAAGGGCCGAGCGCAGAAGTGGTCCTGCAACTTTATCCGCCTCCATCCAGTCTATTAATTGTTGCCGGGAAGCTAGAGTAAGTAGTTCGCCAGTTAATAGTTTGCGCAACGTTGTTGCCATTGCTACAGGCATCGTGGTGTCACGCTCGTCGTTTGGTATGGCTTCATTCAGCTCCGGTTCCCAACGATCAAGGCGAGTTACATGATCCCCCATGTTGTGCAAAAAAGCGGTTAGCTCCTTCGGTCCTCCGATCGTTGTCAGAAGTAAGTTGGCCGCAGTGTTATCACTCATGGTTATGGCAGCACTGCATAATTCTCTTACTGTCATGCCATCCGTAAGATGCTTTTCTGTGACTGGTGAGTACTCAACCAAGTCATTCTGAGAATAGTGTATGCGGCGACCGAGTTGCTCTTGCCCGGCGTCAATACGGGATAATACCGCGCCACATAGCAGAACTTTAAAAGTGCTCATCATTGGAAAACGTTCTTCGGGGCGAAAACTCTCAAGGATCTTACCGCTGTTGAGATCCAGTTCGATGTAACCCACTCGTGCACCCAACTGATCTTCAGCATCTTTTACTTTCACCAGCGTTTCTGGGTGAGCAAAAACAGGAAGGCAAAATGCCGCAAAAAAGGGAATAAGGGCGACACGGAAATGTTGAATACTCATACTCTTCCTTTTTCAATATTATTGAAGCATTTATCAGGGTTATTGTCTCATGACCAAAATCCCTTAACGTGAGTTTTCGTTCCACTGAGCGTCAGACCCCGTAGAAAAGATCAAAGGATCTTCTTGAGATCCTTTTTTTCTGCGCGTAATCTGCTGCTTGCAAACAAAAAAACCACCGCTACCAGCGGTGGTTTGTTTGCCGGATCAAGAGCTACCAACTCTTTTTCCGAAGGTAACTGGCTTCAGCAGAGCGCAGATACCAAATACTGTTCTTCTAGTGTAGCCGTAGTTAGGCCACCACTTCAAGAACTCTGTAGCACCGCCTACATACCTCGCTCTGCTAATCCTGTTACCAGTGGCTGCTGCCAGTGGCGATAAGTCGTGTCTTACCGGGTTGGACTCAAGACGATAGTTACCGGATAAGGCGCAGCGGTCGGGCTGAACGGGGGGTTCGTGCACACAGCCCAGCTTGGAGCGAACGACCTACACCGAACTGAGATACCTACAGCGTGAGCTATGAGAAAGCGCCACGCTTCCCGAAGGGAGAAAGGCGGACAGGTATCCGGTAAGCGGCAGGGTCGGAACAGGAGAGCGCACGAGGGAGCTTCCAGGGGGAAACGCCTGGTATCTTTATAGTCCTGTCGGGTTTCGCCACCTCTGACTTGAGCGTCGATTTTTGTGATGCTCGTCAGGGGGGCGGAGCCTATGGAAAAACGCCAGCAACGCGGCCTTTTTACGGTTCCTGGCCTTTTGCTGGCCTTTTGCTCACATGTTCTTTCCTGCGTTATCCCCTGATTCTGTGGATAACCGTATTACCGCCTTTGAGTGAGCTGATACCGCTCGCCGCAGCCGAACGACCGAGCGCAGCGAGTCAGTGAGCGAGGAAGCGGAAG

Vector Features:

Type

Name

Description

Start

End

bacterial origin

pUC ori

pUC bacterial origin of replication

3161

3834

primer site

M13 reverse

M13 reverse priming site

205

221

primer site

T3 priming site

243

262

primer site

M13 forward

M13 forward priming site

355

370

primer site

T7 priming site

328

347

promoter

lac

lac promoter region

216

selectable marker

AmpR

Ampicillin resistance gene

2203

3063

selectable marker

KanR

Kanamycin resistance gene

1159

1953

Gene

Gene Symbol :

PTPMT1

Symbol Nomenclature :

PTPMT1

SYNONYM :

DUSP23; MOSP; PLIP

Designation :

NB4 apoptosis/differentiation related protein|PTEN-like phosphatase|phosphatidylglycerophosphatase and protein-tyrosine phosphatase 1|phosphoinositide lipid phosphatase|protein-tyrosine phosphatase mitochondrial 1

Full Nomenclature :

protein tyrosine phosphatase, mitochondrial 1

GENEID :

114971

Locus Tag :

PNAS-129

GenBank Accession :

XP_374879

HGNC :

26965

MIM :

609538

Target GenBank:

None

Reference Sequence Alignment

  

HsCD00531500      ---GCGGCCACCGCGCTGCTGGAGGCCGGCCTGGCGCGGGTGCTCTTCTACCCGACGCTG
NM_175732.2       ATGGCGGCCACCGCGCTGCTGGAGGCCGGCCTGGCGCGGGTGCTCTTCTACCCGACGCTG
                     *********************************************************

HsCD00531500      CTCTACACCCTGTTCCGCGGGAAGGTGCCGGGTCGGGCGCACCGGGACTGGTACCACCGC
NM_175732.2       CTCTACACCCTGTTCCGCGGGAAGGTGCCGGGTCGGGCGCACCGGGACTGGTACCACCGC
                  ************************************************************

HsCD00531500      ATCGACCCCACCGTGCTGCTGGGCGCGCTGCCGTTGCGGAGCTTGACGCGCCAGCTGGTA
NM_175732.2       ATCGACCCCACCGTGCTGCTGGGCGCGCTGCCGTTGCGGAGCTTGACGCGCCAGCTGGTA
                  ************************************************************

HsCD00531500      CAGGACGAGAACGTGCGCGGGGTGATCACCATGAACGAGGAGTACGAGACGAGGTTCCTG
NM_175732.2       CAGGACGAGAACGTGCGCGGGGTGATCACCATGAACGAGGAGTACGAGACGAGGTTCCTG
                  ************************************************************

HsCD00531500      TGCAACTCTTCACAGGAGTGGAAGAGACTAGGAGTCGAGCAGCTGCGGCTCAGCACAGTA
NM_175732.2       TGCAACTCTTCACAGGAGTGGAAGAGACTAGGAGTCGAGCAGCTGCGGCTCAGCACAGTA
                  ************************************************************

HsCD00531500      GACATGACTGGGATCCCCACCTTGGACAACCTCCAGAAGGGAGTCCAATTTGCTCTCAAG
NM_175732.2       GACATGACTGGGATCCCCACCTTGGACAACCTCCAGAAGGGAGTCCAATTTGCTCTCAAG
                  ************************************************************

HsCD00531500      TACCAGTCGCTGGGCCAGTGTGTTTACGTGCATTGTAAGGCTGGGCGCTCCAGGAGTGCC
NM_175732.2       TACCAGTCGCTGGGCCAGTGTGTTTACGTGCATTGTAAGGCTGGGCGCTCCAGGAGTGCC
                  ************************************************************

HsCD00531500      ACTATGGTGGCAGCATACCTGATTCAGGTGCACAAATGGAGTCCAGAGGAGGCTGTAAGA
NM_175732.2       ACTATGGTGGCAGCATACCTGATTCAGGTGCACAAATGGAGTCCAGAGGAGGCTGTAAGA
                  ************************************************************

HsCD00531500      GCCATCGCCAAGATCCGGTCATACATCCACATCAGGCCTGGCCAGCTGGATGTTCTTAAA
NM_175732.2       GCCATCGCCAAGATCCGGTCATACATCCACATCAGGCCTGGCCAGCTGGATGTTCTTAAA
                  ************************************************************

HsCD00531500      GAGTTCCACAAGCAGATTACTGCACGGGCAACAAAGGATGGGACTTTTGTCATTTCAAAG
NM_175732.2       GAGTTCCACAAGCAGATTACTGCACGGGCAACAAAGGATGGGACTTTTGTCATTTCAAAG
                  ************************************************************

HsCD00531500      ACA---
NM_175732.2       ACATGA
                  ***

Protein

SMART domain :

DSPc : Dual specificity phosphatase, catalytic domain

Structural Annotation Wiki :

NYSGXRC-8742a

Clone

Cloning Information :

475622

HIP Master Clone ID :

397128

Original Clone ID :

8742a1ZBt10

Experimental

TargetTrack - Experimental data :

NYSGXRC-8742a

Genome

Chromosome :

Map Location :

11p11.2

LOC114971 (H. sapiens )In pCR4zeroBLUNT-TOPO (TOPO cloning vector)

Coding Sequence Details

Recommended Growth Condition:

Vector Features:

Gene

Reference Sequence Alignment

Protein

Clone

Experimental

Genome

LOC114971 (H. sapiens )
In pCR4zeroBLUNT-TOPO (TOPO cloning vector)