Q74GL1_GEOSL (G. sulfurreducens )
In pSGX3 (His-tagged bacterial expression vector)

Explanation of Terms

Gene:
Gene Symbol: 
Gene Name: 
Original Clone ID:
10365t2BCt5p1
Keyword:
full-length cds
Species:
Geobacter sulfurreducens
Type:
cDNA
Vector Name:
pSGX3
Format: 
CLOSED
Source:
New York SGX Research Center for Structural Genomics
Description:
None
Comments:
None
Mutation / Discrepancy:
No / No
Publications:
None
Authors:
PSI
New York SGX Research Center for Structural Genomics
GsCD00531512
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AVAILABLE
No restriction
Special MTA: PSI
Insert sequence: 1944nts
Open reading frame: 1 to 1944

AGCGACCAGACCCTGCAGGACATTCTGCGTTATCTGACGGAAGAGACTGGCCTGGCGCCG
TTTCAGGTGGCCAATACAGTTGAACTGCTGCGCGAGGGTGGGACCGTACCATTCATCGCA
CGTTACCGCAAAGAACGTACGGGAGAACTGGATGAGGTCGGCATTCGCGGTATCGAAGAG
CGTCTGGCTTATTTTACTGAACTGGAAGAGCGCAAACTGACAGTGCTGAAATCTATTGAA
GAGCAAGGGAAACTGACCCCTGAACTGGCGGACCGTATCCGCACGAGTCGTCAGAAAACC
GAAGTTGAGGATCTGTACCTGCCGTATAAACCAAAACGCCGTACTAAAGCCACAATTGCA
CGCGAACGTGGACTGGAGCCTCTGGCTGACCTGATGGCGGCCCAGGAACTGACGGCAGGC
ACCCCGGAAGAGGCTACTCTGCCGTTCGTCGATCCAGCGGGTGAAGTACCTGATGCCGCA
GCTGCGCTGGAAGGCGCCGGTCATATCCTGGCAGAGCGCCTGGCGGACGATGCTGACGCC
CGTGCATTTGTGCGCCGTCTGACAGCGGATCAAGGGATTTTCTGCTCACGCGTTGCTGCC
GACCGTAAAGGAGCAGTCACCAAATTTGAAATGTACTATGATCACCGCGAGCCGCTGAAA
TCCGTGCCGTCGCATCGTATGCTGGCGATGCGCCGTGGCGAAAAAGAAGAGGTACTGGTT
CTGACGATCGAAGCTCCAACCGAGGAAATTCTGGCCGGTCTGCGCGCACGTATCGCGGGC
CGCGCCAGCATTTTCCGTCAGCTGCTGGAAGGTGCAGCGGAGGATGCTTACAAACGCCTG
ATCGCCCCTTCTATTGAAGTGGAGCTGCGTCTGGAAGCGAAACAACGCGCTGACGAAGCA
GCCATCACTGTCTTTGCGCAGAACCTGCGTAATCTGCTGCTGGCTCCGCCAGCAGGGGGA
AAACGCGTTCTGGGCGTAGATCCGGGTCTGCGTACAGGGAGTAAACTGGCCGCGGTGGAC
GGCACGGGTCGCTTCCTGGAGCACGTCACCATTTATCCTCATGCTGGAGGCGAAGGTAAA
GTTGCAGCCGCGAAACGTGAGTTTCTGCGCCTGGTGGAATGTCACGGGATTGAAATGGTA
GCTGTCGGCAACGGTACTGCAGGACGTGAGATGGAACAGTTTGCCAAAGAAACACTGGCG
GAGGGCGGTAAACGCATCCCGGTTGTGATGGTATCAGAAGCTGGGGCATCCGTTTATTCG
GCCAGCGAGATTGCGCGTGAAGAGTTCCCAGATCTGGATCTGACGGTGCGCGGAGCTATC
TCTATTGCACGTCGCCTGCAAGACCCGCTGGCCGAGCTGGTCAAAATCGATCCTAAAAGT
ATTGGCGTTGGTCAGTACCAACATGACGTGGATCAGCGTGCGCTGAAAAAATCACTGGAC
GCAGTAGTCGAATCCTGCGTTAATTATGTGGGGGTAGATCTGAACACCGCCTCGTGGGCG
CTGCTGAGCTACGTCTCTGGCGTTGGTCCGAGTCTGGCACGCGCGATCGTGCGTCACCGC
GATGAGCATGGACCATTTCCGGCTCGTCGCGCCCTGATGAAAGTACCTCGTTTCGGCGAC
AAAGCGTTTGAACAGGCTGCAGGTTTCCTGCGCATTCGTGGGGGCGAACACCCACTGGAT
GCCACTGCGGTCCATCCGGAGCGCTATCCTGTTGTGGAAGCTATGGCAACGGACCTGGGT
GTATCACTGTCCCAACTGGCCGCTGATCCGGCTCTGCCAGCACGTATCGATCTGAAACGC
TACGTCACCGACGCCGTTGGACTGCCGACACTGCGTGATATTATGGAGGAACTGAAAAAA
CCTGGCCGCGACCCGCGTGAAGAGTTTCGCACCGCGGCTTTCCGTGATGACGTGACTGAA
GGGCATCATCACCATCACCACTGA

Protein sequence predicted based on nucleotide sequence

SDQTLQDILRYLTEETGLAPFQVANTVELLREGGTVPFIARYRKERTGELDEVGIRGIEE
RLAYFTELEERKLTVLKSIEEQGKLTPELADRIRTSRQKTEVEDLYLPYKPKRRTKATIA
RERGLEPLADLMAAQELTAGTPEEATLPFVDPAGEVPDAAAALEGAGHILAERLADDADA
RAFVRRLTADQGIFCSRVAADRKGAVTKFEMYYDHREPLKSVPSHRMLAMRRGEKEEVLV
LTIEAPTEEILAGLRARIAGRASIFRQLLEGAAEDAYKRLIAPSIEVELRLEAKQRADEA
AITVFAQNLRNLLLAPPAGGKRVLGVDPGLRTGSKLAAVDGTGRFLEHVTIYPHAGGEGK
VAAAKREFLRLVECHGIEMVAVGNGTAGREMEQFAKETLAEGGKRIPVVMVSEAGASVYS
ASEIAREEFPDLDLTVRGAISIARRLQDPLAELVKIDPKSIGVGQYQHDVDQRALKKSLD
AVVESCVNYVGVDLNTASWALLSYVSGVGPSLARAIVRHRDEHGPFPARRALMKVPRFGD
KAFEQAAGFLRIRGGEHPLDATAVHPERYPVVEAMATDLGVSLSQLAADPALPARIDLKR
YVTDAVGLPTLRDIMEELKKPGRDPREEFRTAAFRDDVTEGHHHHHH*

Coding Sequence Details

Insert Sequence Verified?:
Y
Verification Method:
Sequence Verification
Mutations:
None
Discrepancy:
None
5' Linker Sequence:
None
3' Linker Sequence:
None

Recommended Growth Condition:

Distributed in bacterial strain:
DH5-alpha T1 phage resistant
Antibiotic Selection:
Host Type:  bacterial    Marker:  kanamycin
Bacterial Selection Condition: 50ug/mL kanamycin
Growth Condition: Growth with the single antibiotic in LB at 37 degrees is recommended.
Comments: Conditions for Gateway-type vectors in recombined (with insert) form and other kanamycin resistant vectors.
Protein Expression Results:
ProteinExpressed: Protein_Confirmed
ProteinPurified: Protein_Purified
SolubleProtein: Protein_Soluble
Find experimental protocols in NYSGXRC-10365t
Recommended expression in:
Not Applicable