Gene:
Gene Symbol:
BF3084
Gene Name:
beta-galactosidase
Original Clone ID:
12014a4BCt9p1
Keyword:
full-length cds
Species:
Bacteroides fragilis
Type:
cDNA
Vector Name:
pSGX3
Format:
CLOSED
Source:
New York SGX Research Center for Structural Genomics
Description:
None
Comments:
None
Mutation / Discrepancy:
No / No
Publications:
None
Authors:
PSI
New York SGX Research Center for Structural Genomics
New York SGX Research Center for Structural Genomics
BfCD00531559
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AVAILABLE
No restriction
Special MTA: PSI
Special MTA: PSI
Insert sequence: 2916nts
Open reading frame: 1 to 2916
GGGGATTATGAACAGTCACCCTATTATCTCAACCTCAACGGTAAATGGAAATTCCATTGG
GTGAAAAATCCTGATCTCCGTCCGAAAGACTTTTATAAACCCTCGTTCTATACCGGAGGC
TGGGCAGATATCAACGTTCCGGGAAACTGGGAGCGCCAGGGATACGGAACGGCCATCTAC
GTAAATGAGACTTATGAATTTGATGACAAAATGTTCAACTTTAAGAAGAATCCCCCTCTT
GTGCCTTATAAGGAGAACGAAGTAGGATCTTATCGCCGTACTTTCACTGTGCCTGCCGGA
TGGAAGGGCCGCCGGGTAGTACTCTGCTGCGAAGGTGTGATTTCTTTTTATTATGTGTGG
GTGAACGGACATTTTCTCGGTTACAACCAAGGTTCCAAGACAGCTGCCGAATGGGATATC
ACCGATCAGTTGGAAGAAGGTGAGAATACGATTGCCCTCGAAGTATATCGCTGGAGTTCA
GGTTCCTATCTGGAGTGTCAGGATATGTGGCGTCTGAGTGGTATTGAGCGTGATGTGTAT
CTGTATAGTACTCCCAAACAGTATATAGCCGATTATAAGGTAAACGCAACTCTTGAAAAG
GAACGTTATAAAGATGGTATTTTCGGACTCGACGTTACGGTCGGAGGGCCTGCAGACGGT
GTGGCATCCGTATCTTATACACTGAACGATCCGCTCGGACGTCCTGTACTGTCGGGTGAG
ATGCCTGTCAAGTCGCGCGGACTGAGTAACTTCATCACATTCGGAGAACAGCGCCTGAAG
GATGTGAAACGTTGGAGTGCCGAGCATCCCAATCTCTACACCCTCGTGTTGGAGTTGAAA
AATGCAGGAGGACAGGTGACCGAAGTCACCGGTTGTGAAGTCGGTTTCCGTACTTCGGAG
ATCAAAGACGGACGTTTCTGCATCAACGGTGTGCCTGTATTGGTCAAAGGAACCAATCGT
CATGAACATTCGCAGTTGGGGCGTACCGTCAGCAAAGAGCTCATGGAGCAAGATATACGT
CTGATGAAACTGTATAATATCAATACTGTGCGCAACTCACATTATCCCACCGATCCGTAT
TGGTATCGGCTGTGCGATCGTTACGGACTTTATATGATCGATGAAGCGAATATCGAGTCA
CACGGTATGGGATATGGACCCGCTTCGCTTGCCAAAGACAGCACTTGGCTGACAGCACAC
ATGGATCGTACACATCGCATGTATGAACGTTCGAAAAATCATCCTGCCATCGTTATCTGG
TCATTGGGCAACGAAGCCGGAAACGGAATCAATTTCGAGCGTACCTACGATTGGCTGAAA
TCGGTAGAGAAAAGCCGTCCCGTCCAGTACGAACGTGCCGAACAGAATTACAATACCGAT
ATCTATTGTCGAATGTATCGCAGTGTCGACGAAATCAAGGCCTATCTGGCCCAGAAAGAT
ATCTACCGTCCGTTCATTCTTTGTGAATATGTGCATGCCATGGGTAACAGTGTAGGCGGC
CTGAAAGAGTACTGGGATGTATTCGAGAATAATCCGATGGCACAGGGTGGCTGTGTGTGG
GACTGGGTAGACCAGTCGTTCCGTGAGATCGACTCAAACGGTCGCTGGTACTGGTCGTAT
GGTGGAGACTACGGACCGAAGGGAATTCCGAGCTTCGGTAATTTTTGCTGCAACGGTCTG
GTGAGTGCCGATCGTGTGCCCCATCCGCATTTACTCGAAGTGAAAAAAATCTATCAGAAC
ATCAAATGCACCTTGATCAATAAGAACAATCTGACCGTAAGGGTGAAAAACTGGTTCGAC
TTCTCTAACCTCAACGAATATATCCTCCACTGGCAGGTGGTGGGTGACAATGGCAAATTG
CTGGCCGAAGGTAACAAAGAGGTGAACTGTGCGCCACACGCTACAGCCGATGTGACTTTG
GGAAAAGTTGCCTTGCCTGCCAATGTCCGTGAGGGTTATCTTAATCTGAGCTGGACCCGC
AAAGAAGCTTCACCGATGGTTGGCACCGATTGGGAGGTGGCTTACGACCAATTTGTGTTG
CCCGGAACCAAAGGTAGTACAGCCTATCTGCCTGCTAAGGCCGGGCAGACAGCTTTCACG
GTGGATAAAGAAACCGGTGCTCTCAACTCATTGACACTGGATGGACAAGAATTGCTGGCA
ACTCCTGTTACGCTGAGTTTGTTCCGTCCCGCTACGGATAATGATAACCGTGATCGTAAT
GGTGCATACCTTTGGCGTAAAGCCGGACTCAATCAGTTGACCCAGAAAGTGGTGTCGCTG
AAAGACGGCAAGAAAGCCGCTACTGCGAAAGTGGAGATTCTGAATGCGAAAGGGATGAAA
GTGGGTGATGCCGATTTCGCCTATTCACTAAACTCTGCCGGAGCCTTGAAGGTGAAAGTG
ACTTTCCGGCCCGATACTGCCGTGGTGAAATCGATGGCACGCCTGGGGCTTACTTTCGAG
ATGAATGATACGTATGGCAATGTAGCTTATCTGGGTCGGGGCGACAACGAGACTTATTCC
GACCGTATGCAGTCGGGCAAGATCGCTCTGTATCAGACTACGGCCGAACGTATGTTTCAT
TACTACGTCACTCCGCAGTCTACCGGGAACCGTACGGATGTCCGCTGGATGAAGCTGACG
GACGAAACCGGACAGGGCATCTTTGTCGATTCCAACCGTCCTTTCCAGTTCAGTGTCATC
CCCTTTGCCGATGATGTATTGGAAAAAGCCCGCCACATCAACGACCTCGAACGTAACGGT
CATGTCACCGTACATCTGGATGCCGAACAGGCCGGTGTGGGAACGGCAACCTGCGGGCCG
GGCGTACAGCCGCAATATCGGGTTCCTGTGACGGAACAAAGCTTTGAGTTCACGCTGCGT
ACAGTGAAGGAAGGGCATCATCACCATCACCACTGA
Protein sequence predicted based on nucleotide sequence
GDYEQSPYYLNLNGKWKFHWVKNPDLRPKDFYKPSFYTGGWADINVPGNWERQGYGTAIY VNETYEFDDKMFNFKKNPPLVPYKENEVGSYRRTFTVPAGWKGRRVVLCCEGVISFYYVW VNGHFLGYNQGSKTAAEWDITDQLEEGENTIALEVYRWSSGSYLECQDMWRLSGIERDVY LYSTPKQYIADYKVNATLEKERYKDGIFGLDVTVGGPADGVASVSYTLNDPLGRPVLSGE MPVKSRGLSNFITFGEQRLKDVKRWSAEHPNLYTLVLELKNAGGQVTEVTGCEVGFRTSE IKDGRFCINGVPVLVKGTNRHEHSQLGRTVSKELMEQDIRLMKLYNINTVRNSHYPTDPY WYRLCDRYGLYMIDEANIESHGMGYGPASLAKDSTWLTAHMDRTHRMYERSKNHPAIVIW SLGNEAGNGINFERTYDWLKSVEKSRPVQYERAEQNYNTDIYCRMYRSVDEIKAYLAQKD IYRPFILCEYVHAMGNSVGGLKEYWDVFENNPMAQGGCVWDWVDQSFREIDSNGRWYWSY GGDYGPKGIPSFGNFCCNGLVSADRVPHPHLLEVKKIYQNIKCTLINKNNLTVRVKNWFD FSNLNEYILHWQVVGDNGKLLAEGNKEVNCAPHATADVTLGKVALPANVREGYLNLSWTR KEASPMVGTDWEVAYDQFVLPGTKGSTAYLPAKAGQTAFTVDKETGALNSLTLDGQELLA TPVTLSLFRPATDNDNRDRNGAYLWRKAGLNQLTQKVVSLKDGKKAATAKVEILNAKGMK VGDADFAYSLNSAGALKVKVTFRPDTAVVKSMARLGLTFEMNDTYGNVAYLGRGDNETYS DRMQSGKIALYQTTAERMFHYYVTPQSTGNRTDVRWMKLTDETGQGIFVDSNRPFQFSVI PFADDVLEKARHINDLERNGHVTVHLDAEQAGVGTATCGPGVQPQYRVPVTEQSFEFTLR TVKEGHHHHHH*
Coding Sequence Details
Insert Sequence Verified?:
Y
Verification Method:
Sequence Verification
Mutations:
None
Discrepancy:
None
5' Linker Sequence:
None
3' Linker Sequence:
None
Recommended Growth Condition:
Distributed in bacterial strain:
DH5-alpha T1 phage resistant
Antibiotic Selection:
Host Type:
bacterial
Marker:
kanamycin
Bacterial Selection Condition:
50ug/mL kanamycin
Growth Condition:
Growth with the single antibiotic in LB at 37 degrees is recommended.
Comments:
Conditions for Gateway-type vectors in recombined (with insert) form and other kanamycin resistant vectors.
Protein Expression Results:
ProteinExpressed: Protein_Confirmed
ProteinPurified: Protein_Purified
SolubleProtein: Protein_Soluble
Find experimental protocols in NYSGXRC-12014a
Recommended expression in:
Not Applicable
Vector Name:
pSGX3
Synonyms:
pSGX-3, pSGX3_BC
Sequencing Primer:
Forward:
T7
Reverse:
T7 terminator
Description:
Bacterial (E. coli) expression vector with T7 promoter, adds C-terminal 6xHis tag; kanamycin resistance; TOPO cloning.
Comments:
Modified from pET26b from Novagen
Size (bp):
5339
Parent Vector:
None
Properties:
TOPO Cloning, bacterial expression, in vitro transcription, with tag/fusion/marker
Author Name:
New York SGX Research Center for Structural Genomics
Publications:
None
Vector Map:
Vector Sequence:
Sequence:
Vector Features:
Type
Name
Description
Start
End
ATG start
start
initiation start (Met, Ser, Leu)
5072
5079
TOPO recognition site
TOPO
TOPO cloning site
5076
5085
bacterial origin
ori
pBR322-type bacterial origin of replication
1421
2103
gene fragment
ROP
ROP
2527
2703
promoter
T7
T7 promoter
4983
5003
repressor protein gene
LacI
LacI CDS
3515
4531
selectable marker
KanR
kanamycin resistance gene
563
1375
tag
His
6xHis tag
5086
5103
trxn regulatory element
lac operator
lac operator
5003
5025
trxn termination sequence
T7 term
T7 terminator
5231
5307
