BT_3050 (B. thetaiotaomicron VPI-5482 )
In pSGX3 (His-tagged bacterial expression vector)

Explanation of Terms

Gene:
Gene Symbol:  BT_3050
Gene Name:  chitinase
Original Clone ID:
11092o1BCt2p1
Keyword:
full-length cds
Species:
Bacteroides thetaiotaomicron VPI-5482
Type:
cDNA
Vector Name:
pSGX3
Format: 
CLOSED
Source:
New York SGX Research Center for Structural Genomics
Description:
None
Comments:
Linker not verified.
Mutation / Discrepancy:
No / No
Publications:
None
Authors:
PSI
New York SGX Research Center for Structural Genomics
BtCD00531565
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AVAILABLE
No restriction
Special MTA: PSI
Insert sequence: 921nts
Open reading frame: 51 to 971

CTAGAAATAATTTTGTTTAACTTTAAGAAGGAGATATACATATGTCCCTTAACTGCTTCA
TTAGTTCGTATGTACGGGGTAATTTCTACAATCGCGGACGTATTTCTGCCGAGAGTTTAA
GGGCGAGTGATGATTTGATTTTTCTGAACGTCCGTCCCAATAAAGACGGGTCGCTTTCGT
TTGAGAATCCCCGTATCTTTGAGAATGGGAAAGGAGTGACTAGTTGGGAGGAATTGATAA
AGTCTGTCCGTGCGGATGTGAAAGGAACAAAGACAAAACTTCGTCTGGGCGCTGCCAGTG
GCGAGTGGAAAGCGATGGTTGCAGATGAGGCGGCCCGTGTTGCATTTGCCAAAAACATAA
AGAAGATACTGGAAAAGAATAAGCTCGATGGCATCGATCTGGATTTTGAATGGGCGGAGA
CTGAAAAAGAGTATGAAGATTACAGTCTGGCTATTCTGAAGATGAGAGAGGTATTGGGAG
ATAAATATGTGTTCAGCGTATCCCTGCATCCTGTGTGTTATAAGATTAGTAAAGAGGCGA
TTGCTGCGGTCGATTTCATTTCTTATCAGTGTTACGGTCCTTCGCCGGTTCGCTTCCCGA
TGGAGAAATACTGTTCGGATATACAGATGGCGTTGGCTTATGGCATTCCAAAGGAAAAGC
TGGTGGCAGGCGTTCCTTTCTATGGCGTGACAAAAGATGGCTCAAAGAAGACTGAGGCTT
ACTTCAGTTTTGTGCAGGACGGGCTGATTACGGGGCCGGCACAAAACGAAGTAACCTATA
AAGGAGACGTGTATGTCTTTGACGGGCAAAATAATATCCGTGCCAAGACACGTTATGCGA
TGGACCAGCAGCTCAAAGGAATGATGAGCTGGGACTTGGCTACGGATATGCCGCTGAATG
ACAGCAGATCGTTGTTTAAGACGATGGTGGAGGAGTTGGGGAGGGAAGGGCATCATCACC
ATCACCACTGATCCGAATTCGAGCTCCGTCGACAAGCTTGCGGCCGCACTCGAGCACCAC
CA

Protein sequence predicted based on nucleotide sequence

NCFISSYVRGNFYNRGRISAESLRASDDLIFLNVRPNKDGSLSFENPRIFENGKGVTSWE
ELIKSVRADVKGTKTKLRLGAASGEWKAMVADEAARVAFAKNIKKILEKNKLDGIDLDFE
WAETEKEYEDYSLAILKMREVLGDKYVFSVSLHPVCYKISKEAIAAVDFISYQCYGPSPV
RFPMEKYCSDIQMALAYGIPKEKLVAGVPFYGVTKDGSKKTEAYFSFVQDGLITGPAQNE
VTYKGDVYVFDGQNNIRAKTRYAMDQQLKGMMSWDLATDMPLNDSRSLFKTMVEELGREG
HHHHHH*

Coding Sequence Details

Insert Sequence Verified?:
Y
Verification Method:
Sequence Verification
Mutations:
None
Discrepancy:
None
5' Linker Sequence:
None
3' Linker Sequence:
None

Recommended Growth Condition:

Distributed in bacterial strain:
DH5-alpha T1 phage resistant
Antibiotic Selection:
Host Type:  bacterial    Marker:  kanamycin
Bacterial Selection Condition: 50ug/mL kanamycin
Growth Condition: Growth with the single antibiotic in LB at 37 degrees is recommended.
Comments: Conditions for Gateway-type vectors in recombined (with insert) form and other kanamycin resistant vectors.
Protein Expression Results:
ProteinExpressed: Protein_Confirmed
ProteinPurified: Protein_Purified
SolubleProtein: Protein_Soluble
Find experimental protocols in NYSGXRC-11092o
Recommended expression in:
Not Applicable