BC1864 (B. cereus ATCC 14579 )
In pSGX3 (His-tagged bacterial expression vector)

Explanation of Terms

Gene:
Gene Symbol:  BC1864
Gene Name:  DNA polymerase I
Original Clone ID:
11085b5BCt2p1
Keyword:
full-length cds
Species:
Bacillus cereus ATCC 14579
Type:
cDNA
Vector Name:
pSGX3
Format: 
CLOSED
Source:
New York SGX Research Center for Structural Genomics
Description:
None
Comments:
Linker not verified.
Mutation / Discrepancy:
No / No
Publications:
None
Authors:
PSI
New York SGX Research Center for Structural Genomics
BcCD00531623
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AVAILABLE
No restriction
Special MTA: PSI
Insert sequence: 2133nts
Open reading frame: 1 to 2133

GATATAGAAGAAGCACGTACTACAGGGATTATCGATGATAAAGAAGCGGAAAGCCGAAGT
GAGAGCTTAGAAAAAGCGTATTTAAAAACACCATTGGATCCGTGGAAAGGTGAAATTTGT
ACGGTATCGCTGTCAGCGGCGGCACACGAGTCAAGAGTTGTTCCAATCTCACATAAAGTT
GGCCAAGTATTTGAACCAAGTATGGATAGAGATGAAGCTAGGAAATTAGTTCTAGATTTG
CTTGATGAATACCTATTTAAAAATGAAAAGGTATTAAAGATTGCGGTCAATTTGTCTTTT
GAAACGAAATATGCAGCGAAGTACGGTAAATACATTTTAGGAAAAGTAGCAGATCCATTG
ATTATGTGGGTACGGTGTTTACAAATCGCAGCACCTCAAAAGATTAATAACCCGAAAAAA
CCTACAAGCGGATGGGGTTTAAAACCAGCTACGAAACACATTTTTGGTGTAACGATGAAC
GACTTTTCAGCCCTTTTAAAGAAATACAAGGTCAATTTCTTTGACGAAATTGATGCGAGT
AAAGGTGAAGGGTTGCTTTACTCAGCTGAAGACTCGGATTATGCTGTGCAGCATTACGAA
TATTGGTCTCAAATTGCAGCTCAAATTTCGCGATATGAGGAATGGCTTCATAAGATTGAA
ATGCCATTCACACGTGTTATCGGCCTTATGGAATATTGGGGGATGAATTGGGATCCAAAC
CTTGCAACGCAAAAGAAACAAGAAGCAGAAATTATGCAAGAGCAAGCGGCTGAACGTATT
AAACAAATCGCGAAAGAAACGTTCAATATTGATATAAATACCGGTAAATCAGGTAAAACG
AACGAAGTGAAAAGCTTAATGTTTGATTACTTAAAAATACCGATTGCCAAATATGGAAAA
ACAGGCGCGAGTCTTGATCAAGAGGCGCTTATCGATATGGCATTTATGCTTGAAAACAAG
CTGAACGATATCGACGAGGAAAAATACCTCGGCGTTTCATTACCTGAAAATTGGGAAAAT
ATCGATCCGGAAAAGGATCCTACTTTAGATAAGCTAGAACGCGGAGCGATTCGTATCGCA
AAACGTGAACCGCATCCCTATAAAGAACAGGCACTAGAAGTTATTGACCAGCTAAAGAAA
ATACAAAAATACACGACATTACTTTCTTCGCACATCATAGGACGTGAAAAGTACTTGAAC
TTTATGAGTGGACGGATCCATGCAGGGTACAGTCCATTCACAGAAACAGGACGCTTAAAC
AGTTTTAATCCGAACGGACAAAACGTACCGAGACCAGATAATGATGAGTTTAAGATTAGA
AACTTCTTTGTACCTAAACCAGGAAAGATATTATTTTTCATTGACTTCTCAGGGTTTGAG
CTTCGCTTAATGGCATGGAAATCAGGTGACGAGGTCATGACGGAGCTATTTAATACAGGC
GGGGATATGCACCGTAGAACCGCATCTGTAATGACTGGAAAGTCTGAGGCTGAAATTGTA
AAGAAAGAACGTACAGATGCCAAGGCGGGCAACTTCGGTATCTCTTATGGCGGTACGGAA
CACGCTCTACAGTTCACATTTAAAACGAAATACATGATTCGTAAAACATTAGATGAATGT
GCGCAGATTGTGAATGCCGTTAAAACAGCGTACAAACGCATACCAGAGTATCAACGCAAG
ATTGTTTTAGAAGCACGGGAGCAAGGCTATGTACAAACGATTTATGGATATATGAGATTA
CTACCTGGCATTAACAGCGCGAATAGAAGAGATCGTGGCTCTGCTGAAAGACAAGCAGCG
AATACACCAGTACAAGGAAGTGCCGCTGACATTATGAAGAAAGTACAAAATGAAATTTAT
GAATCTATCGGTAAACAGGAAGGCGTACTTTCTCATGGAAGTGCCGATATGATTGCACAA
ATTCACGATGAAATCATTTTTGAAATAGATGATGATCCGGAAATTGTAGTTGCAGTAGAA
AAACAAATTAAACAAGTTATGGAGCAACCTCCAGTACCAGGATTTCCAGTTCCGATTGAG
GCAGAAGGAAGTGTAGGTTATCGATGGGGCGAGAAAATGAGTGTTGAGTCCTGGCTTAAA
CAAAGGGAAGGGCATCATCACCATCACCACTGATCCGAATTCGAGCTCCGTCGACAAGCT
TGCGGCCGCACTCGAGCACCACCA

Protein sequence predicted based on nucleotide sequence

DIEEARTTGIIDDKEAESRSESLEKAYLKTPLDPWKGEICTVSLSAAAHESRVVPISHKV
GQVFEPSMDRDEARKLVLDLLDEYLFKNEKVLKIAVNLSFETKYAAKYGKYILGKVADPL
IMWVRCLQIAAPQKINNPKKPTSGWGLKPATKHIFGVTMNDFSALLKKYKVNFFDEIDAS
KGEGLLYSAEDSDYAVQHYEYWSQIAAQISRYEEWLHKIEMPFTRVIGLMEYWGMNWDPN
LATQKKQEAEIMQEQAAERIKQIAKETFNIDINTGKSGKTNEVKSLMFDYLKIPIAKYGK
TGASLDQEALIDMAFMLENKLNDIDEEKYLGVSLPENWENIDPEKDPTLDKLERGAIRIA
KREPHPYKEQALEVIDQLKKIQKYTTLLSSHIIGREKYLNFMSGRIHAGYSPFTETGRLN
SFNPNGQNVPRPDNDEFKIRNFFVPKPGKILFFIDFSGFELRLMAWKSGDEVMTELFNTG
GDMHRRTASVMTGKSEAEIVKKERTDAKAGNFGISYGGTEHALQFTFKTKYMIRKTLDEC
AQIVNAVKTAYKRIPEYQRKIVLEAREQGYVQTIYGYMRLLPGINSANRRDRGSAERQAA
NTPVQGSAADIMKKVQNEIYESIGKQEGVLSHGSADMIAQIHDEIIFEIDDDPEIVVAVE
KQIKQVMEQPPVPGFPVPIEAEGSVGYRWGEKMSVESWLKQREGHHHHHH*

Coding Sequence Details

Insert Sequence Verified?:
Y
Verification Method:
Sequence Verification
Mutations:
None
Discrepancy:
None
5' Linker Sequence:
None
3' Linker Sequence:
None

Recommended Growth Condition:

Distributed in bacterial strain:
DH5-alpha T1 phage resistant
Antibiotic Selection:
Host Type:  bacterial    Marker:  kanamycin
Bacterial Selection Condition: 50ug/mL kanamycin
Growth Condition: Growth with the single antibiotic in LB at 37 degrees is recommended.
Comments: Conditions for Gateway-type vectors in recombined (with insert) form and other kanamycin resistant vectors.
Protein Expression Results:
ProteinPurified: Not_Tested
ProteinExpressed: Protein_Confirmed
SolubleProtein: Tested_Not_Soluble
Find experimental protocols in NYSGXRC-11085b
Recommended expression in:
Not Applicable