Atu3356 (A. tumefaciens )
In pSGX3 (His-tagged bacterial expression vector)

Explanation of Terms

Gene:
Gene Symbol:  Atu3356
Gene Name:  malic enzyme
Original Clone ID:
6228b3BCt4b1
Keyword:
None
Species:
Agrobacterium tumefaciens
Type:
cDNA
Vector Name:
pSGX3
Format: 
CLOSED
Source:
New York SGX Research Center for Structural Genomics
Description:
None
Comments:
Linker not verified.
Mutation / Discrepancy:
No / No
Publications:
None
Authors:
PSI
New York SGX Research Center for Structural Genomics
AtCD00531689
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AVAILABLE
No restriction
Special MTA: PSI
Insert sequence: 2310nts
Open reading frame: 51 to 2360

TTCCTCAGAAAATTTTGTTTACTTTAGAAGGAGATATACATATGTCCCTTTCCGACACGA
CGAAAACCGCCAGCACAAGAACATCGGTTACGGATCAGGAAGCCCTGGATTTCCACGCGC
AGGGCAGGCCGGGCAAACTGGAAATCACCCCGACCAAGCCGATGGCAACGCAGCGGGATC
TGTCGCTTGCCTATTCGCCCGGTGTTGCTGTGCCGGTGAAAGCCATCGCCGAAAATCCGG
CGACCGCTTACGATTATACCACCCGCGGCAACATGGTGGCCGTCATCTCGAACGGCACCG
CCATCCTCGGGCTCGGCAATCTCGGCGCGCTCGCCTCCAAGCCGGTCATGGAAGGCAAGT
CGGTCCTCTTCAAGCGTTTCGCCGATGTCGATTCCATCGATCTCGAGGTCGATACCGAGG
ACGCCGATGAATTCATAAATTGCGTGCGCTACCTTGGCCCCTCCTTCGGCGGTATCAATC
TCGAAGACATCAAGGCGCCGGAATGTTTCATCATCGAAAGCCGCCTGCGCGAGCTGATGG
ACATTCCCGTCTTCCATGACGACCAGCACGGCACCGCCATCATCGCCGCCGCCGGCCTCA
TCAACGCCATCGAACTTACCGGCCGCGACTTCAAGACGACAAAGCTCGTCTGCAATGGCG
CAGGCGCTGCCGCGATTGCCTGCATGGACCTCATCAAGGCCATGGGTTTCAACCCGGAAA
ACATCACGCTCTGCGATACCAAGGGCGTGATCTATCAGGGCCGCACCGAAGGCATGAACC
AGTGGAAATCCGCCCATGCGGTGAAGACCGACAGACGGACGCTGACGGAGGCGATGAACG
GTGCGGATGTGGTGTTCGGCCTGTCGCAGAAGGGCGCTTTCACTGAAGAGATGATCCGCT
CTATGGCGCCGAAGCCGATCATTTTCGCCATGGCCAACCCGGATCCGGAAATCACGCCGG
AAGAAGTCTCGCGCATCCGCGACGACGCGATCATGGCGACCGGGCGTTCTGACTACCCGA
ACCAGGTCAACAACGTCCTCGGTTTCCCCTATATCTTCCGCGGCGCGCTCGACGTGCGTG
CCCGCCAGATCAACGACGCGATGAAGATTGCCGCCGCACAGGCGCTGGCCGATCTTGCCC
GCGAAGATGTGCCTGACGACGTGGCCGCCGCCTATCAGGGCAACCGCCCGCGCTTCGGGT
CGCAATACATCATTCCGGTTCCCTTCGATCCGCGCCTGATCTCGGCCATTCCGGTGGCCG
TGGCCAAAGCCGCCATCGAAACCGGCGTCGCCCAGCGCGAAATACCCGATCTCGACGCCT
ATGCCCGCGAGCTTTCCGCCCGCCGCGACCCGATGGCCTCTACGACGCAGCGCCTTTACG
AGCGTGTGCGCCGTTCGCCCAAGCGCGTGGTCTTTGCCGAAGCGGAAGAAGAGCAGGTCA
TGCGCGCGGCGATTTCGTTCACGGCGCAGGGTCTCGGCACAGCCATCCTGCTCGGCCGTG
ACGATATCATTAAGGCCAATGCAGAACGCGCGGGCATCGATCTCGACCGCGCCAATATCG
AAATCACCAATGCCCGTCTCTCCAGCCGCGTCGATGCCTATGTCGATTATCTCTATGCGC
GTCTGCAGCGCGGCGGTTTCCTGCTGCGCGACGTGCAGCGTCTCATCCATAACGACCGCA
ACCATTTCGCCGCCTGCATGGTGGCGATGGGCGATGCGGATGCCGTGGTGACCGGCGTTA
CCCGCAATTATTCCACCGCGCTGGAGGATATCCGCCGCTGTATCAACACCAAGCCTGGCC
ATCGCGTCATCGGCGTTTCGCTTGTTGTCTCCCGAAATCGCACCGTCTTCGTCGCTGATA
CCGCCGTGCACGACATGCCGAATGCGGAAGACCTTGCCGATATCGCGGAAGAGGCGGCCC
ACCTCGCCCGCCGTTTCGGTTATGAGCCACGCGTCGCCATGCTTGCCTATTCCACCTTCG
GCCAGCCGGTCGGTGAACGCTCCGACAAGGTGCGCGAAGCGGTGAAAATCCTCGACAAGC
GCAATGTCAATTTCGAGGTGGACGGCGAGATGTCGGCGGATGTGGCGCTGAACCATCAGC
GCATGCAGAGCCAGTATCCCTTTGCCCGCCTTTCCGGTGCGGCCAACGTGCTGGTCATGC
CCGCCATCCATTCCGCCTCCATCTCCACCAAGATGCTGCAGGAACTGGGCGGCGCGACCG
TCATCGGTCCGCTTCTCGTCGGCCTCGACAAATCGGTGCAGATCACCTCCATGGGTGCCA
AGGACAGCGATATCGTCAACATGGCGGCCATCGCAGCCTATAACGCCGGCCGAGAAGGGC
ATCATCACCATCACCACTGATCCGAATTCGAGCTCCGTCGACAAGCTTGCGGCCGCACTC
GAGCACCACCA

Protein sequence predicted based on nucleotide sequence

SDTTKTASTRTSVTDQEALDFHAQGRPGKLEITPTKPMATQRDLSLAYSPGVAVPVKAIA
ENPATAYDYTTRGNMVAVISNGTAILGLGNLGALASKPVMEGKSVLFKRFADVDSIDLEV
DTEDADEFINCVRYLGPSFGGINLEDIKAPECFIIESRLRELMDIPVFHDDQHGTAIIAA
AGLINAIELTGRDFKTTKLVCNGAGAAAIACMDLIKAMGFNPENITLCDTKGVIYQGRTE
GMNQWKSAHAVKTDRRTLTEAMNGADVVFGLSQKGAFTEEMIRSMAPKPIIFAMANPDPE
ITPEEVSRIRDDAIMATGRSDYPNQVNNVLGFPYIFRGALDVRARQINDAMKIAAAQALA
DLAREDVPDDVAAAYQGNRPRFGSQYIIPVPFDPRLISAIPVAVAKAAIETGVAQREIPD
LDAYARELSARRDPMASTTQRLYERVRRSPKRVVFAEAEEEQVMRAAISFTAQGLGTAIL
LGRDDIIKANAERAGIDLDRANIEITNARLSSRVDAYVDYLYARLQRGGFLLRDVQRLIH
NDRNHFAACMVAMGDADAVVTGVTRNYSTALEDIRRCINTKPGHRVIGVSLVVSRNRTVF
VADTAVHDMPNAEDLADIAEEAAHLARRFGYEPRVAMLAYSTFGQPVGERSDKVREAVKI
LDKRNVNFEVDGEMSADVALNHQRMQSQYPFARLSGAANVLVMPAIHSASISTKMLQELG
GATVIGPLLVGLDKSVQITSMGAKDSDIVNMAAIAAYNAGREGHHHHHH*

Coding Sequence Details

Insert Sequence Verified?:
Y
Verification Method:
Sequence Verification
Mutations:
None
Discrepancy:
None
5' Linker Sequence:
None
3' Linker Sequence:
None

Recommended Growth Condition:

Distributed in bacterial strain:
DH5-alpha T1 phage resistant
Antibiotic Selection:
Host Type:  bacterial    Marker:  kanamycin
Bacterial Selection Condition: 50ug/mL kanamycin
Growth Condition: Growth with the single antibiotic in LB at 37 degrees is recommended.
Comments: Conditions for Gateway-type vectors in recombined (with insert) form and other kanamycin resistant vectors.
Protein Expression Results:
ProteinPurified: Not_Tested
ProteinExpressed: Protein_Confirmed
SolubleProtein: Tested_Not_Soluble
Find experimental protocols in NYSGXRC-6228b
Recommended expression in:
Not Applicable