Gmet_0833 (G. metallireducens GS-15 )
In pSGX3 (His-tagged bacterial expression vector)

Explanation of Terms

Gene:
Gene Symbol:  Gmet_0833
Gene Name:  alpha-amylase family protein
Original Clone ID:
11094g4BCt11p1
Keyword:
None
Species:
Geobacter metallireducens GS-15
Type:
cDNA
Vector Name:
pSGX3
Format: 
CLOSED
Source:
New York SGX Research Center for Structural Genomics
Description:
None
Comments:
Linker not verified.
Mutation / Discrepancy:
No / No
Publications:
None
Authors:
PSI
New York SGX Research Center for Structural Genomics
GmCD00531714
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AVAILABLE
No restriction
Special MTA: PSI
Insert sequence: 1812nts
Open reading frame: 51 to 1862

TCCCTCTGAATATTTTGTTTACTTTAGAAGGAGATATACATATGTCCCTTTCGCCCGTGG
CCTGGGAAGACCAGGTGCTCTACTTTCTCCTGCTCGACCGCTTTTCCGACGGGAAGGAGA
CCGGATACCGCGACAACCGGGGGAGGCGGGTCGCGAAGGGGGAAACGCCCCTTTACCAGC
CAGGCGACGCGGGGAATGCCATCCGCACCGAGCCCGAGGCTGCCGTCTGGCGCGAGTCGG
GGAGCCGCTGGTGCGGCGGAACCCTGAAGGGGCTAGCCAGCAAGATGGGGTATCTCAAGC
GCATGGGAGTGACCGCCGTCTGGGTCAGCCCCCTCTTCAAGCAGTGCTCCTTCGTCCCTA
CCTACCACGGCTACGGCATCCAGAACTTCCTCGAGGTGGACCCCCATTTCGGCACCGCCG
ACGACCTGCGGGAACTGGTCCATGTTGCCCATGCCAACGGCATCTACGTCATCCTCGACA
TCATCCTGAACCACGCCGGCAATATCTTTGCCTACGACCGGGGCTATACCCCCTATGACG
GCTCCACCGTCTACCCGGTGACGGGGTTTCGCTCCGCCGACGGCACGCCGAACCTCCCCT
TCGGCCCCATCGATACCAAGGCCAACCCCGATGCCTGGCCCGATGGCGCGGTCTGGCCCC
GGGAGCTTCAGGCGGCCAACGCCTATACCCGCATGGGCCACATCCGGGATTGGGACAGCT
ACCACGAATACCTGGACGGTGACTTCTTCGACCTGAAGGACATCCACCTGGGTGAGGGCG
AGGTTGATGCTTTCCGTCCGTCGCCGGCACTCCTGGCCCTCTGCGAGGTTTACAAGTACT
GGATTGCCCTCTCCGATGTGGACGGATACCGCATCGACACGGTGAAGCACATGGGTGCCG
GGGCCACCCGGTTTTTCGCCTCGGTGATCCACGAGTTCGCCCAGGCCATCGGCAAGGAGA
ACTTTTACCTCATCGGCGAGATCACCGGCGGCCGCGACAATGCCTTCAACACCCTGGCGA
CCACCGGCCTCAACGCGGCACTCGGCGTGGACGACATTCCGCTGCGCCTTGAGGACCTGG
TGAAAGGGAGGGCAAACCCCGCCGACTACTTCAACCTCTTCCGCAACTCCCTCCTGGTGC
GGAAGGAGTCCCACGTCTGGTTCCGGGACAAGGTGGTGACGGTCATCGACGACCACGACC
AGGTCTGTCGGGGGGCCAAGAAGGAGCGTTTCTGCGCCGGCGATCCCAAGTGGCGCCGGC
TCGTGCTGAACGCCCTGGCCCTGAACACGACCACCCTCGGCATCCCCTGCGTCTACTATG
GCACCGAGCAGGCCTTTGACGGCGAGGGGGGCGATGACCGGTATCTGCGCGAGTGCATGT
TCGGCGGTCCTTTCGGCGCGTTCAGAACCCGGGGCGTCCAATTTTTTGACGAGGAAAACC
CGGTCTACCGCGAGTTCGCGAAGATTCTCGCCCTGCGCCGGGAGAAGCTCCCCCTGCGGC
GCGGGCGCCAGTATCTGCGGGAGATCTCGGGGGACGGGGTCACTTTCGGGCTTCCCTATG
TCATCGGGGGCGAGATTCGCTCGGTGGTCCCCTGGTCCCGCATCTTTGACACCGAAGAGA
TGGTTCTTGCCATCAACACCGATGCCGACTCCCCCCGCACCGCCTGGGTGCTGGTTGACG
GGAGCCTCCACCGGGAGGGTGACCGCTTCGCCTACCGCTACTCCACCGATCCGGGGCAGG
TACGCGGAACAACCGCTGTCCAGCGGATCAACGATGCCGTTCAGGCGATACAGGTGACGG
TTCCCGCAGCCGGTTTCGTGATGCTTGAACAGCGAGAAGGGCATCATCACCATCACCACT
GATCCGAATTCGAGCTCCGTCGACAAG

Protein sequence predicted based on nucleotide sequence

SPVAWEDQVLYFLLLDRFSDGKETGYRDNRGRRVAKGETPLYQPGDAGNAIRTEPEAAVW
RESGSRWCGGTLKGLASKMGYLKRMGVTAVWVSPLFKQCSFVPTYHGYGIQNFLEVDPHF
GTADDLRELVHVAHANGIYVILDIILNHAGNIFAYDRGYTPYDGSTVYPVTGFRSADGTP
NLPFGPIDTKANPDAWPDGAVWPRELQAANAYTRMGHIRDWDSYHEYLDGDFFDLKDIHL
GEGEVDAFRPSPALLALCEVYKYWIALSDVDGYRIDTVKHMGAGATRFFASVIHEFAQAI
GKENFYLIGEITGGRDNAFNTLATTGLNAALGVDDIPLRLEDLVKGRANPADYFNLFRNS
LLVRKESHVWFRDKVVTVIDDHDQVCRGAKKERFCAGDPKWRRLVLNALALNTTTLGIPC
VYYGTEQAFDGEGGDDRYLRECMFGGPFGAFRTRGVQFFDEENPVYREFAKILALRREKL
PLRRGRQYLREISGDGVTFGLPYVIGGEIRSVVPWSRIFDTEEMVLAINTDADSPRTAWV
LVDGSLHREGDRFAYRYSTDPGQVRGTTAVQRINDAVQAIQVTVPAAGFVMLEQREGHHH
HHH*

Coding Sequence Details

Insert Sequence Verified?:
Y
Verification Method:
Sequence Verification
Mutations:
None
Discrepancy:
None
5' Linker Sequence:
None
3' Linker Sequence:
None

Recommended Growth Condition:

Distributed in bacterial strain:
DH5-alpha T1 phage resistant
Antibiotic Selection:
Host Type:  bacterial    Marker:  kanamycin
Bacterial Selection Condition: 50ug/mL kanamycin
Growth Condition: Growth with the single antibiotic in LB at 37 degrees is recommended.
Comments: Conditions for Gateway-type vectors in recombined (with insert) form and other kanamycin resistant vectors.
Protein Expression Results:
ProteinPurified: Not_Tested
ProteinExpressed: Protein_Confirmed
SolubleProtein: Tested_Not_Soluble
Find experimental protocols in NYSGXRC-11094g
Recommended expression in:
Not Applicable