PG1750 (P. gingivalis W83 )
In pSGX3 (His-tagged bacterial expression vector)

Explanation of Terms

Gene:
Gene Symbol:  PG1750
Gene Name:  alpha-1,3/4-fucosidase
Original Clone ID:
11093j6BCt28p1
Keyword:
None
Species:
Porphyromonas gingivalis W83
Type:
cDNA
Vector Name:
pSGX3
Format: 
CLOSED
Source:
New York SGX Research Center for Structural Genomics
Description:
None
Comments:
Linker not verified.
Mutation / Discrepancy:
No / Yes
Publications:
None
Authors:
PSI
New York SGX Research Center for Structural Genomics
PhCD00531761
Price: 
Login for Pricing
AVAILABLE
No restriction
Special MTA: PSI
Insert sequence: 1704nts
Open reading frame: 51 to 1754

TCCCTCTGAAAATTTTGTTAACTTTAGAAGGAGATATACATATGTCCCTTGCGGCAAAGG
TAGTCCCTACCCTACAACAGCTGGCTTGGCAGCAGATGGAGCTGACGGCATTTCTGCACT
TCGGCATCAATACTTTTACCGACAGTGAGTGGGGTGATGGGAAAGAAGATCCGGCACTCT
TCAACCCGGTGGAGCTGGATGCTCGGCAATGGGTACGAATACTGCACGAAGCGGGCTTTC
GCATGGTAATCCTTACGGCCAAGCACCACGATGGTTTTTGTCTCTGGCCTACAGCCACCA
CGCGCCATTCGGTGGCTTCATCTCCGTGGCGAGAGGGCAAAGGCGATGTGGTGAAAGAAG
TCCGAGCAGCGTGCGAAGAGTATGGTATGAAGTTCGGCATTTATCTGTCTCCTTGGGATC
GCAATGCGGAGTGCTATGGCGATTCGCACCGTTACAATCGGTTTTTTGTCAGTCAGCTCA
CCGAACTGCTCACTCACTATGGAGAAGTACACGAAGTATGGTTCGATGGAGCCAATGGCG
AAGGGCCCAACGGCAAGCGACAGGAATATGATTGGGAGACATTCTACGACACCATCCGCC
GACTGCAACCCCAAGCCGTTATGGCTATCATGGGAGACGATGTGCGCTGGGTAGGCAATG
AGCGTGGACTGGGACGAACCACCGAGTGGAGTGCCACAGTGCTTACGCCGGGTATTTATT
CCCGTTCGAAGACGGAACGCAATCGTCTGGGAATTAGGGAGAACTCTCCGGATTTGGGCA
GCCGAGAGGTGCTGAAAGAAGCTGGCGAAATCTTTTGGTATCCGTCCGAAGTGGACGTTT
CTATTCGTCCGGGTTGGTTTTACCATGCTGCCGAGGACAAGAAAGTCAAGTCATTGGACA
AGTTGGTGGATATTTACTTTCAGTCCGTAGGTTATAATTCGGTACTCCTGCTCAATGTGC
CGCCCGATAGGCGAGGCCTGATCCACGAAGCCGATGCCTTGCGCCTGAAGGAGTGGGCTG
ACTATCTCGGTCGGGCCTTTGCTCATGACAGGGTAGTGGATTCGGCGCGCTATGCCGTTG
TTCAAGAACATGCCGTCGAAGAATATGCACTCGAATCTAAGACGCACATCAATATACTTA
TGCTCCAAGAGGATATTACTAAGGGGCAGCGCACCGAGGCTTTCACTGTGGAGGCGTGGG
TGGATGGAGCATGGCAGGAGATCGGCCGCGGTACGACTATCGGCTATAAGCGACTTCTTC
GCATTCCTGCAGTCGAGACGGATCGGATACGGGTACGTATAGAACAGTGTCGTCTGCCGG
TCAATCTGTGTCGCGTAGCTGTGTATTATGCCGAGCCACCGGAGCAGGTCGTTGCCGATG
AGTCGTGGAACGATATTCCACGTGCAGACTGGAAGCTGCTTTCGGACGATCCATTGACTA
TCGATCTGGGTAGAGAGGTCATGTTGCGCGCTTTTACCTATGCGCCATATAAGGCCGAAG
CCAAGCCTACGACTGCCGTCCGCTATCGGCTGGCTGTGAGTGAAGATGGCAGACAGTGGC
GAGAGATCCCGACTATGGGGGAGTTTGCCAATGTGATCAATAATCCTCTGCCTCAAACTG
TAGATATGAATCAAATCGTCCGTACGCGCTTCATCCGCTTGGATGCAACTGCCGCATCGG
GAGATAAAGCCGTAATCACGATGCAGGAAATCGGCGTGACTGTGGAAGAAGGGCATCATC
ACCATCACCACTGATCCGAATTCGAGCTCCGTCGACAAGCTTGCGGCCGCACTCGAGCAC
CACCA

Protein sequence predicted based on nucleotide sequence

AAKVVPTLQQLAWQQMELTAFLHFGINTFTDSEWGDGKEDPALFNPVELDARQWVRILHE
AGFRMVILTAKHHDGFCLWPTATTRHSVASSPWREGKGDVVKEVRAACEEYGMKFGIYLS
PWDRNAECYGDSHRYNRFFVSQLTELLTHYGEVHEVWFDGANGEGPNGKRQEYDWETFYD
TIRRLQPQAVMAIMGDDVRWVGNERGLGRTTEWSATVLTPGIYSRSKTERNRLGIRENSP
DLGSREVLKEAGEIFWYPSEVDVSIRPGWFYHAAEDKKVKSLDKLVDIYFQSVGYNSVLL
LNVPPDRRGLIHEADALRLKEWADYLGRAFAHDRVVDSARYAVVQEHAVEEYALESKTHI
NILMLQEDITKGQRTEAFTVEAWVDGAWQEIGRGTTIGYKRLLRIPAVETDRIRVRIEQC
RLPVNLCRVAVYYAEPPEQVVADESWNDIPRADWKLLSDDPLTIDLGREVMLRAFTYAPY
KAEAKPTTAVRYRLAVSEDGRQWREIPTMGEFANVINNPLPQTVDMNQIVRTRFIRLDAT
AASGDKAVITMQEIGVTVEEGHHHHHH*

Coding Sequence Details

Insert Sequence Verified?:
Y
Verification Method:
Sequence Verification
Mutations:
None
Discrepancy:
t429c,L143L;t822c,A274A;
c870t,F290F;a891g,S297S;

a909g,V303V;g1037a,G346E;
g1089t,L363L;g1364a,R455K;

a1530g,G510G;g1593t,R531R
                                                    

5' Linker Sequence:
None
3' Linker Sequence:
None

Recommended Growth Condition:

Distributed in bacterial strain:
DH5-alpha T1 phage resistant
Antibiotic Selection:
Host Type:  bacterial    Marker:  kanamycin
Bacterial Selection Condition: 50ug/mL kanamycin
Growth Condition: Growth with the single antibiotic in LB at 37 degrees is recommended.
Comments: Conditions for Gateway-type vectors in recombined (with insert) form and other kanamycin resistant vectors.
Protein Expression Results:
ProteinExpressed: Protein_Confirmed
ProteinPurified: Protein_Purified
SolubleProtein: Protein_Soluble
Find experimental protocols in NYSGXRC-11093j
Recommended expression in:
Not Applicable