BT_3096 (B. thetaiotaomicron )
In pSGX3 (His-tagged bacterial expression vector)

Explanation of Terms

Gene:
Gene Symbol:  BT_3096
Gene Name:  alpha-L-arabinofuranosidase
Original Clone ID:
12032a4BCt5p1
Keyword:
None
Species:
Bacteroides thetaiotaomicron
Type:
cDNA
Vector Name:
pSGX3
Format: 
CLOSED
Source:
New York SGX Research Center for Structural Genomics
Description:
None
Comments:
Linker not verified.
Mutation / Discrepancy:
No / Yes
Publications:
None
Authors:
PSI
New York SGX Research Center for Structural Genomics
BtCD00531762
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AVAILABLE
No restriction
Special MTA: PSI
Insert sequence: 2091nts
Open reading frame: 1 to 2091

CGTAAACCACATGAAAAGACCCTGAAAACCAAATTTTGCAGAACGGCAGTGTATTGCCTG
TGCTGTTTTATGTTTATCCAGCCGATAACAGGAAGCCAGGTAAATGATACTCATGAGGGT
GTTTTGCATATCGACAAACAGAAAACACGCAAAGTAAGCCGTGTCCAATATGGGTTCCAT
TATGAAGAGATTGGTATGATAGGAGAAGGTGCCTTGCATGCCGAACTGGTACGCAACCGT
TCATTCGAAGAAGCAACTCCACCCGCAGATCTGGCGGTCAAAAATGGGCTTTATCAGAAT
GTACCGAATCCGCGTGGAAAAAACAAAGATGTATTTCATGTAGACCCTCTGATCGGATGG
AACACTTATCCTCTGTCTTATACTCCCATTTTCATTAGCCGTACAGAAGAAAATCCTCTG
AATAAGGAGAATAAGTATTCAATGCTGGTCAACGTGACTGAAGATATTGCAAACAATCCG
GAGGCAATGATTCTAAATCGGGGATATTACGGTATGAATCTACGAAAGGAGGTATCTTAT
CATCTTTCCATGTATATAAAAAGCAAAAATTATACAGCTCTTCTTCAAGTTATGCTGGTA
GACGAACAAGGTAAACCGGTCAGTACTCAGCTTGTTTTGGATGTAAAGGGTAAAGAGTGG
ACGAAACTTACCGGAACATTAAAACCTGATAAAGATGTCAAACGGGGGATGCTTGCCATT
CAGCCGTTAGGTAAAGGGCAATTCCAACTGGATGTCGTCTCTTTATTCCCTTCTGATACA
TGGGACAATGGAAAATCAGTTTTTCGGGCGGATATTATGCAGAACTTGAAAGAATATGCA
CCGGATTTCATTCGTTTTCCCGGCGGATGTATTGTGCATGGGGTGAATGAAGCTACCATG
TACCACTGGAAAAAGACAATAGGTCCCATTGAAAATCGACCGGGACAATGGAGTAAATGG
GCGCCTTACTACAGTACAGATGGAATTGGTTATCATGAGTTTTACGAATTGTGCGAATAT
TTGGGAGCAGATGCTATGTACGTAATCCCTACAGGGATGATCTGTACAGGTTGGGTAAAG
CAAAGTTCTCCCTGGAATTTCATTCAACCGGACGTTGATCTGGATGCTTATATTCAGGAT
GCACTGGATGCCATAGAATACGCAATCGGTCCGGAAACCAGCAAATGGGGGGCTTTACGT
GTAAAGAACGGACATCCGAAGCCTTTCCCTCTAAAGTACATTGAAATTGGAAACGAAGAC
TTCGGACCTGTTTATTGGGAACGTTACGAGAAGATTTATCAAGCGTTACACAGACAGTAC
CCCGACTTGATATACATAGCCAACAGTATCATAGGCAAAGAAAATGACGACAAACGGATT
GATATTGCGAAGTTTGTGAACCCGAAGAATGTGAAGGTATTCGATGAACACCATTATCAA
CCTGTCGAATGGGCTTGCAAGCAGCATTACCGTTTTGACAACTATGAACGGGGAATAGCG
GATTTGTTTGTTGGAGAATTGGGCATTGACGGGAAGTATCCCTATAATCTGTTAGCCAGC
GGAGCGGTGCGAATGTCTCTTGAACGTAATGGAGACTTGAATCCATTATTGGCAGAACGT
CCGGTGATGCGCCATTGGGATTTTTCAGAGCATCGTCAGTTGCACTCTATGCTAATCAAT
GGGGTAGATTGCAGCATCAGGACATCTTTCTATTATTTATCAAAAATGTTCAGAGACCAC
ACTTTCGATGTATGCCTGGATGCTGGGATTCAGGGGATGAAGGGTTTGCAGAATGTCTTT
GTTACGATGGGATATGATAGTGAAAGTAAAGAATATATCCTGAAACTGATCAATTTGAGA
AAACAGGAGGTGGTACTTCGAACAGCAGTGAAAGGATTTGGGAAAAAGATACAGGCACAA
AAAATAACATTGAGTCTGGAACCCGGCAAGAATAACACTCCGGAAATGCCGGATGCAGTA
AAGCCAGTTCAGTCGACCGTAACACTGGACTTAAATAAAGATTTGCAGCTTGAGGCTTCT
TCAATGATTGTATACCGTTTTAAAGAAGGGCATCATCACCATCACCACTGATCCGAATTC
GAGCTCCGTCGACAAGCTTGCGGCCGCACTCGAGCACCACCA

Protein sequence predicted based on nucleotide sequence

RKPHEKTLKTKFCRTAVYCLCCFMFIQPITGSQVNDTHEGVLHIDKQKTRKVSRVQYGFH
YEEIGMIGEGALHAELVRNRSFEEATPPADLAVKNGLYQNVPNPRGKNKDVFHVDPLIGW
NTYPLSYTPIFISRTEENPLNKENKYSMLVNVTEDIANNPEAMILNRGYYGMNLRKEVSY
HLSMYIKSKNYTALLQVMLVDEQGKPVSTQLVLDVKGKEWTKLTGTLKPDKDVKRGMLAI
QPLGKGQFQLDVVSLFPSDTWDNGKSVFRADIMQNLKEYAPDFIRFPGGCIVHGVNEATM
YHWKKTIGPIENRPGQWSKWAPYYSTDGIGYHEFYELCEYLGADAMYVIPTGMICTGWVK
QSSPWNFIQPDVDLDAYIQDALDAIEYAIGPETSKWGALRVKNGHPKPFPLKYIEIGNED
FGPVYWERYEKIYQALHRQYPDLIYIANSIIGKENDDKRIDIAKFVNPKNVKVFDEHHYQ
PVEWACKQHYRFDNYERGIADLFVGELGIDGKYPYNLLASGAVRMSLERNGDLNPLLAER
PVMRHWDFSEHRQLHSMLINGVDCSIRTSFYYLSKMFRDHTFDVCLDAGIQGMKGLQNVF
VTMGYDSESKEYILKLINLRKQEVVLRTAVKGFGKKIQAQKITLSLEPGKNNTPEMPDAV
KPVQSTVTLDLNKDLQLEASSMIVYRFKEGHHHHHH*

Coding Sequence Details

Insert Sequence Verified?:
Y
Verification Method:
Sequence Verification
Mutations:
None
Discrepancy:
a22c,M8L;a30c,T10T;
c973a,R325S;a1880g,Q627R
                                                    

5' Linker Sequence:
None
3' Linker Sequence:
None

Recommended Growth Condition:

Distributed in bacterial strain:
DH5-alpha T1 phage resistant
Antibiotic Selection:
Host Type:  bacterial    Marker:  kanamycin
Bacterial Selection Condition: 50ug/mL kanamycin
Growth Condition: Growth with the single antibiotic in LB at 37 degrees is recommended.
Comments: Conditions for Gateway-type vectors in recombined (with insert) form and other kanamycin resistant vectors.
Protein Expression Results:
ProteinExpressed: Protein_Confirmed
SolubleProtein: Protein_Soluble
ProteinPurified: Tested_Not_Purified
Find experimental protocols in NYSGXRC-12032a
Recommended expression in:
Not Applicable