Gene:
Gene Symbol:
Gene Name:
None
Original Clone ID:
11042e2BCt14p1
Keyword:
None
Species:
Sulfolobus solfataricus
Type:
cDNA
Vector Name:
pSGX3
Format:
CLOSED
Source:
New York SGX Research Center for Structural Genomics
Description:
None
Comments:
Linker not verified.
Mutation / Discrepancy:
No / Yes
Publications:
None
Authors:
PSI
New York SGX Research Center for Structural Genomics
New York SGX Research Center for Structural Genomics
SsCD00531767
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AVAILABLE
No restriction
Special MTA: PSI
Special MTA: PSI
Insert sequence: 3510nts
Open reading frame: 51 to 3560
TCCCTCTGAAAATTTTGTTAACTTTAGAAGGAGATATACATATGTCCCTTAATGTAATGT
ATAAAAATTCCTGCCCTAACTGTGGTGGGGACATTTCTGGTGACAGATTGCTGAATGGTC
TGCCATGTGAGGCTTGTTTACCTTATATTAACGGAATAGATGATGTAGACCATATTTCAA
AGGTAAAAACGTTATACAATATTTTACTTAATAACGATAAAATTAAAAATTATTGGAATT
TATATTATAACATCACTACATTTGAAACTGTCTTTAAATATTTTAGAGATAAAACTGGAT
ATGAACCTTGGTCTCTCCAGAAACTATGGTTAAGAAGGCTTGCGAGTAATCAGAGTTTCA
CTATGTCAGCTCCAACTGGATTGGGGAAGACAACCACATTGATGACCTATTCTGTCTTTA
TTGGCCAAGATGTAGTATATATCGTTCCCACAAAGTCCTTAATGGAACAAGTATGTAAAA
GATTAGAGAAATTAGGTGCTCAAGTATCTTGTGGTAAAATTGATCAAAAGAAAGTAAGCG
TAATAACAATAAGTTACCTAAATAAGAACGCGGACTCGATAGTAAGCCTTAAGCCAAACT
TTGTCGTAATAGATGATGCAGATGCCGTAATAAAGAGCGGTAAAACAACTGATAGACTTG
TTAGCTTATTGGGGATTCCGAAGGACGTTTATGAGAGTGCGATTCAACTAATTAGGCTAA
GGAATAAATACTATTTCTCTAACGAATACACGGAAGAAGTTAAAGAAAAAATTAGAGAAT
TGGAGCTTAAGATAGCAGAATTTAAGGATAAAATTTCACAGCTAGTTATAGCTAGTGCTA
CAATACGACCTAAAGGCATTAAGCAAAAAGCTTTAAGGTTATTAACAGGTTTTGAACCTT
CTTCTATTCAGTTATATGCTAGGAATATAATTGATGCTTATACTGAAAATCTAGATCTTT
CAATAGTCAAGGAATTAGGAAGTGGTGGGTTAATTCTAGTTTCGAAAGAATATGGTAGGT
CCAAATTAAATGAAATAAAGAAATACGTTGAGGACTTAGGTTTTAACGCTAAATTAGCAA
TAAGTGGAAGGAAATTCCTCGACGATTTCTCTCAAGGTAAAGTAGATATTCTAGTAGGTT
CTGCGTCTTATTATGGTGTTGCCGTTAGAGGAATTGATGAACCTAAAAGATTAAAATACG
TCATATATTATGGAGTACCTAAAATAAGAGCTAAGCTTTTTGACGCTTTATCTAATCCAT
TTACATTACTCAGAGTAGGTAAATTAATTGGAATCGATTTTTCAAAGTTACAGAATAAGA
TTTTAATTCTGAATCCTTCTGAAGTTCAATTATTGAAGTTTTCCATAATAAAAGGAGAAA
GTATAAACTACCATAAGTTAGAACAATTGAGGCAAGAATTGCTATATTATATGTCATTAG
TTAAAGATAAGTTGAAGGAGAAAGTCGAGGATACTTTAATTTCAGATAGTTTTGTAATTA
CCAAGCAAAATGGAAATTATTATATCGTCTATCCCGATATGATAACATATTTGCAAGGCT
CTGGGAGGGCAAGCAGACTTTACAATGGTGGATTGACTCTTGGATTTTCAATAATTCTAG
TAGATGATAGACACATCTTTGAAATATTGGAGAAGAAAATGCAAAAATTGTTTCCTAACA
CTACCTTTACTTCATTATCTAATACAAATTTATCTGAAATAAAAATTAAACTGGAAGACT
CTAGAAAAGAAGAAGGGAATAGGGTCCATTTTAACATATCAACTGGTTTACTTATAGTAG
AGTCTCCAACAAAAGCAAAGACTATAGCTAAAATGTTTAGCAGACCCTCGGTTAGGATAA
GAAATAAAGTACCAGTATACGAAACCATTATAGTAGATGGTAATCAAATATACGTGTTAG
ATATAGTAGCTTCAAAAGGCCATATCGTGGATCTAACCTTAGAAGATATAGGATATTATG
GGATAAAAGTAGAAGATGATGGAACTATAAAGCCATATTACGACTTAATAAAGAAATGCC
TCGACTGTAATAAGATATTTTCTAGTGCTTCAGATAAATGTCCATACTGTGGTTCTGCAA
ATCTTCAATCAGCTCAAACTACCATTAACTTACTCAGGGAGTTAGCGTTATCAGTAGATA
AAGTGTTTATAGCTTCTGACCCAGATACTGAAGGGGAGAAGATAGCATATGATCTGGCTT
CATTCTTGTCTCCATATAATTCCAACGTGTATAGAGTAGTATATCACGAAATTACTAAGA
AGGCAATATTAGAGGCATTGAGGAATCCCAAAAAGATTAATACAAATCTAGTAATGAGTC
AGATAGTTAGAAGGATAGAAGATAGATGGATTGGCTTCACGTTAAGTAACTTACTAAAAA
CGAAGTTTAATGGACATAATCATGGTGCGGGTAGGGTACAAACTCCAGTTTTAGGTTGGA
TAGTGAATAAAACGATAAAGTATAAGAGTGCAATGGGTCACGTGGTTTATATTGACATCG
CGGGATACTCCATTAAAAAATATTTTAGTGAAAAAAGGAAGGTAGAAGAGTATATAAATA
ACTTACAAGTAGCTAGAATTGAAAAAATCTCTGAAGAGAAAATACTATTATCTCCATTAC
CTCCCTTTACTACAGACACCTTACTTATTGAGGCTAACATGAAATATAAACTACCAGCTA
ATCTTGTAATGAAGATTGCTCAAGATCTATTTGAGGCTGGCCTAATAACATATCATAGAA
CTGATAGTACTCATATTTCCTCTGTAGGTATAGAAATAGCAAAGGAGTATCTTCAAAAGC
AAGCGCTCATTAAAGAGTTTGTTCCTAGATCATGGGAATCATCGGAGGAAGGTGCTCATG
AGGCAATAAGACCAACTAGAGCTATAGACGTTAACGAGTTAATTCAAGAAATAGAGGAGA
ATCCTTATAAGTATTCGATAAGGTTTAGTAAATTACACTTATTAATTTACGACTTAATAT
TCAAGCGATTTATAGCTAGTCAGATGTCCCATGCAGTTGGTACAAAAAGTAGGTATTTGA
TAAAGTTAGGCAAGGACGATAATGTCGATGTAGAATTGTTAAGTAATGCTGAAGGCGGAT
TTATTAAGGTATATCCAGTTAAGGTGTATAATTTACCTGTAGGTGAGATTCAACCTAAAG
TTAATATTACTAAGGGGTCAAGTGAACAATTGCTAAGTTACTCTGATGTGATATCATTAA
TGAAATCTAAGGGAATTGGAAGGCCTAGTACATATGCAAAAACTATAGAAAATCTAATGA
AGCATGGATATATAGTTAGCAGTAAAAGAAAGTCATATCTAATAGCAACAAACAGAGGTA
TCTCAGTATATCAATTTCTAAGCTCCAAATTCTACGATCTAGTTTCCGAAAGTACCACAT
CTAGATTAATGACAAAATTGGACGACATTGCACTAAGCAAGTTGAATGCTTCTACAGTGT
TATTAGAAATATTCAGCGAGATATCTACCTTAGTAAACCCTCTTAAGTCTGAGGAAGGGC
ATCATCACCATCACCACTGATCCGAATTCGAGCTCCGTCGACAAGCTTGCGGCCGCACTC
GAGCACCACCA
Protein sequence predicted based on nucleotide sequence
NVMYKNSCPNCGGDISGDRLLNGLPCEACLPYINGIDDVDHISKVKTLYNILLNNDKIKN YWNLYYNITTFETVFKYFRDKTGYEPWSLQKLWLRRLASNQSFTMSAPTGLGKTTTLMTY SVFIGQDVVYIVPTKSLMEQVCKRLEKLGAQVSCGKIDQKKVSVITISYLNKNADSIVSL KPNFVVIDDADAVIKSGKTTDRLVSLLGIPKDVYESAIQLIRLRNKYYFSNEYTEEVKEK IRELELKIAEFKDKISQLVIASATIRPKGIKQKALRLLTGFEPSSIQLYARNIIDAYTEN LDLSIVKELGSGGLILVSKEYGRSKLNEIKKYVEDLGFNAKLAISGRKFLDDFSQGKVDI LVGSASYYGVAVRGIDEPKRLKYVIYYGVPKIRAKLFDALSNPFTLLRVGKLIGIDFSKL QNKILILNPSEVQLLKFSIIKGESINYHKLEQLRQELLYYMSLVKDKLKEKVEDTLISDS FVITKQNGNYYIVYPDMITYLQGSGRASRLYNGGLTLGFSIILVDDRHIFEILEKKMQKL FPNTTFTSLSNTNLSEIKIKLEDSRKEEGNRVHFNISTGLLIVESPTKAKTIAKMFSRPS VRIRNKVPVYETIIVDGNQIYVLDIVASKGHIVDLTLEDIGYYGIKVEDDGTIKPYYDLI KKCLDCNKIFSSASDKCPYCGSANLQSAQTTINLLRELALSVDKVFIASDPDTEGEKIAY DLASFLSPYNSNVYRVVYHEITKKAILEALRNPKKINTNLVMSQIVRRIEDRWIGFTLSN LLKTKFNGHNHGAGRVQTPVLGWIVNKTIKYKSAMGHVVYIDIAGYSIKKYFSEKRKVEE YINNLQVARIEKISEEKILLSPLPPFTTDTLLIEANMKYKLPANLVMKIAQDLFEAGLIT YHRTDSTHISSVGIEIAKEYLQKQALIKEFVPRSWESSEEGAHEAIRPTRAIDVNELIQE IEENPYKYSIRFSKLHLLIYDLIFKRFIASQMSHAVGTKSRYLIKLGKDDNVDVELLSNA EGGFIKVYPVKVYNLPVGEIQPKVNITKGSSEQLLSYSDVISLMKSKGIGRPSTYAKTIE NLMKHGYIVSSKRKSYLIATNRGISVYQFLSSKFYDLVSESTTSRLMTKLDDIALSKLNA STVLLEIFSEISTLVNPLKSEEGHHHHHH*
Coding Sequence Details
Insert Sequence Verified?:
Y
Verification Method:
Sequence Verification
Mutations:
None
Discrepancy:
g2514a,V838V
5' Linker Sequence:
None
3' Linker Sequence:
None
Recommended Growth Condition:
Distributed in bacterial strain:
DH5-alpha T1 phage resistant
Antibiotic Selection:
Host Type:
bacterial
Marker:
kanamycin
Bacterial Selection Condition:
50ug/mL kanamycin
Growth Condition:
Growth with the single antibiotic in LB at 37 degrees is recommended.
Comments:
Conditions for Gateway-type vectors in recombined (with insert) form and other kanamycin resistant vectors.
Protein Expression Results:
ProteinPurified: Not_Tested
ProteinExpressed: Protein_Confirmed
SolubleProtein: Tested_Not_Soluble
Find experimental protocols in NYSGXRC-11042e
Recommended expression in:
Not Applicable
Vector Name:
pSGX3
Synonyms:
pSGX-3, pSGX3_BC
Sequencing Primer:
Forward:
T7
Reverse:
T7 terminator
Description:
Bacterial (E. coli) expression vector with T7 promoter, adds C-terminal 6xHis tag; kanamycin resistance; TOPO cloning.
Comments:
Modified from pET26b from Novagen
Size (bp):
5339
Parent Vector:
None
Properties:
TOPO Cloning, bacterial expression, in vitro transcription, with tag/fusion/marker
Author Name:
New York SGX Research Center for Structural Genomics
Publications:
None
Vector Map:
Vector Sequence:
Sequence:
Vector Features:
Type
Name
Description
Start
End
ATG start
start
initiation start (Met, Ser, Leu)
5072
5079
TOPO recognition site
TOPO
TOPO cloning site
5076
5085
bacterial origin
ori
pBR322-type bacterial origin of replication
1421
2103
gene fragment
ROP
ROP
2527
2703
promoter
T7
T7 promoter
4983
5003
repressor protein gene
LacI
LacI CDS
3515
4531
selectable marker
KanR
kanamycin resistance gene
563
1375
tag
His
6xHis tag
5086
5103
trxn regulatory element
lac operator
lac operator
5003
5025
trxn termination sequence
T7 term
T7 terminator
5231
5307
