SO_2498 (S. oneidensis )
In pET21_NESG (His-tagged bacterial expression vector)

Explanation of Terms

Gene:
Gene Symbol:  SO_2498
Gene Name:  bifunctional diguanylate cyclase/phosphodiesterase with GAF and PAS sensory domains
Original Clone ID:
SoR300I-21.16
Keyword:
targeted domain
Species:
Shewanella oneidensis
Type:
cDNA
Vector Name:
pET21_NESG
Format: 
FUSION
Source:
Northeast Structural Genomics Consortium
Description:
None
Comments:
None
Mutation / Discrepancy:
No / No
Publications:
None
Authors:
PSI
Northeast Structural Genomics Consortium
SoCD00531973
Price: 
Login for Pricing
AVAILABLE
No restriction
Special MTA: PSI/NESG
Insert sequence: 549nts
Open reading frame: 51 to 599

AATTCCCCTCTAGAAATAATTTTGTTTAACTTTAAGAAGGAGATATACATATGCGACGTC
AGCATCAGCACTTATTAGAGTCTTTAGTCCATTCTACGGCAAAGCAACAGGGTGATTTCA
CTAAGACGGCAGAGTTAGCGACAGAGCTACTCTGTCAGAATTTAGCGGTTTCTTTGGTGT
CAATATGGATTTTTTCTGCCGATCAAGGCACGCAATCTCTTGTTGCTCAATTTGGCTCCA
TGGCGTTGCAGGACACGTATCGTCCCAGTCTGCTGCATACGGTGCCACGTTATTTAAATG
AACTCAAAAATTATCGCCATATTGATGCAGGCAATACCCTTATCGACCCGCGATTGACCG
AATTAGTCGATAGTTATTTTATTCCACGCCAAATTTTATCCAGTCTTGATGTCGGCATTC
GCATCAATGGTCATCTTGAGGGCGTGCTCTGTATTGAACGGGCGCAGGTCACCCATCATT
GGCACGATAGTGAAATTCATCTTGCTTGCCAAATGGCCGATCAATTAGCCCTCACCCTCG
CCACAAAACATGCTTACGATAAAGAAGAGCGCCTTAGTCTTTTCCGTTGTGCCATAGAAC
TCGAGCACCACCACCACCACCACTGAGATCC

Protein sequence predicted based on nucleotide sequence

MRRQHQHLLESLVHSTAKQQGDFTKTAELATELLCQNLAVSLVSIWIFSADQGTQSLVAQ
FGSMALQDTYRPSLLHTVPRYLNELKNYRHIDAGNTLIDPRLTELVDSYFIPRQILSSLD
VGIRINGHLEGVLCIERAQVTHHWHDSEIHLACQMADQLALTLATKHAYDKEERLSLFRC
AIE

Coding Sequence Details

Insert Sequence Verified?:
Y
Verification Method:
Sequence Verification
Mutations:
None
Discrepancy:
t423c,T141T
                                                    

5' Linker Sequence:
None
3' Linker Sequence:
None

Recommended Growth Condition:

Distributed in bacterial strain:
DH5-alpha T1 phage resistant
Antibiotic Selection:
Host Type:  bacterial    Marker:  ampicillin
Bacterial Selection Condition: 100 ug/mL ampicillin
Growth Condition: Growth with the single antibiotic in LB at 37 degrees is recommended.
Comments: Commonly used conditions for ampicillin resistant plasmid clones.
Protein Expression Results:
ProteinPurified: Not_Tested
ProteinExpressed: Protein_Confirmed
SolubleProtein: Protein_Soluble
Find experimental protocols in SoR300I
Recommended expression in:
Not Applicable