RSP_0960 (C. sphaeroides 2.4.1 )
In pSGX3 (His-tagged bacterial expression vector)

Explanation of Terms

Gene:
Gene Symbol:  RSP_0960
Gene Name:  crotonyl-CoA carboxylase/reductase
Original Clone ID:
11159l1BCt3p1
Keyword:
None
Species:
Cereibacter sphaeroides 2.4.1
Type:
cDNA
Vector Name:
pSGX3
Format: 
CLOSED
Source:
New York SGX Research Center for Structural Genomics
Description:
None
Comments:
None
Mutation / Discrepancy:
No / No
Publications:
None
Authors:
PSI
New York SGX Research Center for Structural Genomics
RsCD00532005
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AVAILABLE
No restriction
Special MTA: PSI
Insert sequence: 1254nts
Open reading frame: 1 to 1254

GACGTGCAGAGCGATATCGTCGCCTACGACGCGCCCAAGAAGGACCTCTACGAGATCGGC
GAGATGCCGCCTCTCGGCCATGTGCCGAAGGAGATGTATGCCTGGGCCATCCGGCGCGAG
CGTCATGGCGAGCCGGATCAGGCCATGCAGATCGAAGTGGTCGAGACGCCCTCGATCGAC
AGCCACGAGGTGCTCGTTCTCGTGATGGCGGCGGGCGTGAACTACAACGGCATCTGGGCC
GGCCTCGGCGTGCCCGTCTCGCCGTTCGACGGTCACAAGCAGCCCTATCACATCGCGGGC
TCCGACGCGTCAGGCATCGTCTGGGCGGTGGGCGATAAGGTCAAGCGCTGGAAGGTGGGC
GACGAGGTCGTGATCCACTGCAACCAGGACGACGGCGACGACGAGGAATGCAACGGCGGC
GACCCGATGTTCTCGCCCACCCAGCGGATCTGGGGCTACGAGACGCCGGACGGCTCCTTC
GCCCAGTTCACCCGCGTGCAGGCGCAGCAGCTGATGAAGCGTCCGAAGCACCTGACCTGG
GAGGAGGCGGCCTGCTACACGCTGACCCTCGCCACCGCCTACCGGATGCTCTTCGGCCAC
AAGCCGCACGACCTGAAGCCGGGGCAGAACGTGCTGGTCTGGGGCGCCTCGGGCGGCCTC
GGCTCCTATGCGATCCAGCTCATCAACACGGCGGGCGCCAATGCCATCGGCGTCATCTCC
GAGGAAGACAAGCGCGACTTCGTCATGGGGCTGGGCGCCAAGGGCGTCATCAACCGCAAG
GACTTCAAATGCTGGGGCCAGCTGCCCAAGGTGAACTCGCCCGAATATAACGAGTGGCTG
AAAGAGGCGCGCAAGTTCGGCAAGGCCATCTGGGACATCACCGGCAAGGGCATCAACGTC
GACATGGTGTTCGAACATCCGGGCGAGGCGACCTTCCCGGTCTCGTCGCTGGTGGTGAAG
AAGGGCGGCATGGTCGTGATCTGCGCCGGCACCACCGGCTTCAACTGCACCTTTGACGTT
CGCTACATGTGGATGCATCAGAAGCGTCTGCAGGGCAGCCACTTCGCCAACCTCAAGCAG
GCGTCGGCCGCCAACCAGCTGATGATCGAGCGCCGCCTCGATCCCTGCATGTCCGAGGTC
TTCCCCTGGGCCGAGATCCCGGCCGCCCATACGAAGATGTACAGAAACCAGCACAAGCCC
GGCAACATGGCGGTGCTGGTGCAGGCCGAAGGGCATCATCACCATCACCACTGA

Protein sequence predicted based on nucleotide sequence

DVQSDIVAYDAPKKDLYEIGEMPPLGHVPKEMYAWAIRRERHGEPDQAMQIEVVETPSID
SHEVLVLVMAAGVNYNGIWAGLGVPVSPFDGHKQPYHIAGSDASGIVWAVGDKVKRWKVG
DEVVIHCNQDDGDDEECNGGDPMFSPTQRIWGYETPDGSFAQFTRVQAQQLMKRPKHLTW
EEAACYTLTLATAYRMLFGHKPHDLKPGQNVLVWGASGGLGSYAIQLINTAGANAIGVIS
EEDKRDFVMGLGAKGVINRKDFKCWGQLPKVNSPEYNEWLKEARKFGKAIWDITGKGINV
DMVFEHPGEATFPVSSLVVKKGGMVVICAGTTGFNCTFDVRYMWMHQKRLQGSHFANLKQ
ASAANQLMIERRLDPCMSEVFPWAEIPAAHTKMYRNQHKPGNMAVLVQAEGHHHHHH*

Coding Sequence Details

Insert Sequence Verified?:
Y
Verification Method:
Sequence Verification
Mutations:
None
Discrepancy:
None
5' Linker Sequence:
None
3' Linker Sequence:
None

Recommended Growth Condition:

Distributed in bacterial strain:
DH5-alpha T1 phage resistant
Antibiotic Selection:
Host Type:  bacterial    Marker:  kanamycin
Bacterial Selection Condition: 50ug/mL kanamycin
Growth Condition: Growth with the single antibiotic in LB at 37 degrees is recommended.
Comments: Conditions for Gateway-type vectors in recombined (with insert) form and other kanamycin resistant vectors.
Protein Expression Results:
ProteinExpressed: Protein_Confirmed
ProteinPurified: Protein_Purified
SolubleProtein: Protein_Soluble
Find experimental protocols in NYSGXRC-11159l
Recommended expression in:
Not Applicable