Pacsin3 (Mus musculus) in pDONR221 (Gateway donor/master vector)

Explanation of Terms

Gene:
Gene Symbol:  Pacsin3
Gene Name:  protein kinase C and casein kinase substrate in neurons 3
Original Clone ID:
5433.1.13_GO.34568
Keyword:
full-length cds
Species:
Mus musculus
Type:
cDNA
Vector Name:
pDONR221
Format: 
CLOSED
Source:
Center for Eukaryotic Structural Genomics
Description:
None
Comments:
Linker not verified.
Mutation / Discrepancy:
No / No
Publications:
None
Authors:
University of Wisconsin Madison
PSI
Center for Eukaryotic Structural Genomics
MmCD00533550
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AVAILABLE
No restriction
Special MTA: PSI
Insert sequence: 1293nts
Open reading frame: 51 to 1343

ATGAGCAATGCTTTTTTATAATGCCAACTTTGTACAAAAAAGCAGGCTCCGAAAACCTGT
ACTTCCAGTCCGCTCCAGAGGAGGACGCCGGAGGGGAGGTCCTAGGGGGCAGTTTCTGGG
AGGCTGGCAACTACAGACGGACGGTGCAGAGGGTTGAGGATGGGCACAGGCTGTGTGGGG
ACCTGGTTAGCTGCTTCCAAGAACGTGCCCGCATTGAGAAGGCCTACGCCCAGCAGCTGG
CTGACTGGGCCCGCAAGTGGAGGGGTGCTGTGGAGAAGGGCCCACAGTACGGCACCCTGG
AGAAGGCCTGGCATGCCTTCTTCACTGCGGCTGAGAGGCTGAGCGAGCTGCACTTGGAGG
TGAGGGAGAAGTTGCACGGTCCAGACAGCGAGCGAGTGCGGACCTGGCAGCGAGGGGCTT
TCCACCGGCCAGTGCTGGGGGGCTTTCGGGAAAGCCGGGCTGCAGAGGATGGTTTCCGTA
AAGCTCAGAAGCCCTGGCTAAAGAGGCTGAAGGAGGTTGAGGCTTCTAAGAAGAGCTACC
ACACAGCCCGCAAGGATGAGAAGACAGCCCAGACCCGGGAGAGCCACGCGAAGGCAGACA
GCTCCATGTCCCAGGAGCAGCTTCGAAAATTACAGGAGCGGGTGGGCCGCTGCACCAAGG
AGGCAGAGAAGATGAAAACCCAGTATGAGCAGACCCTGGCCGAGCTAAATCGCTACACCC
CACGCTACATGGAGGACATGGAGCAGGCTTTTGAGAGCTGCCAGGCTGCTGAGCGCCAGC
GGCTTCTCTTCTTCAAGGATGTCTTGCTCACCCTGCATCAACACCTCGACCTCTCCAGTA
GCGACAAGTTCCATGAACTCCATCGAGACCTGCAGCAGAGCATTGAGGCTGCCAGTGATG
AAGAGGATCTGCGCTGGTGGCGGAGCACACACGGGCCTGGCATGGCCATGAACTGGCCAC
AGTTTGAGGAATGGTCGTTAGACACACAGAGAGCCATCAGCCGCAAGGAGAAGGGTGGCC
GGAGCCCAGATGAGGTTACTCTGACCAGCATTGTACCGACAAGAGATGGCACCGCACCCC
CACCCCAGTCCCCATCGTCCCCGGGTAGCGGGCAGGACGAGGACTGGTCAGATGAGGAGA
GTCCCCGGAAAGTTGCCACTGGGGTGAGGGTACGGGCGCTTTATGACTATGCTGGCCAGG
AGGCTGATGAGCTGAGCTTCCGAGCAGGGGAAGAGTTGCTGAAGATGAGCGAGGAGGATG
AGCAGGGCTGGTGCCAGGGCCAGCTGCAGAGTGGCCGCATTGGTCTGTACCCTGCCAATT
ACGTAGAATGTGTGGGCGCCTGATAGGACCCAGCTTTCTTGTACAAAGTTGGCATTATAA
GAAAGCATTGCTTA

Protein sequence predicted based on nucleotide sequence

ENLYFQSAPEEDAGGEVLGGSFWEAGNYRRTVQRVEDGHRLCGDLVSCFQERARIEKAYA
QQLADWARKWRGAVEKGPQYGTLEKAWHAFFTAAERLSELHLEVREKLHGPDSERVRTWQ
RGAFHRPVLGGFRESRAAEDGFRKAQKPWLKRLKEVEASKKSYHTARKDEKTAQTRESHA
KADSSMSQEQLRKLQERVGRCTKEAEKMKTQYEQTLAELNRYTPRYMEDMEQAFESCQAA
ERQRLLFFKDVLLTLHQHLDLSSSDKFHELHRDLQQSIEAASDEEDLRWWRSTHGPGMAM
NWPQFEEWSLDTQRAISRKEKGGRSPDEVTLTSIVPTRDGTAPPPQSPSSPGSGQDEDWS
DEESPRKVATGVRVRALYDYAGQEADELSFRAGEELLKMSEEDEQGWCQGQLQSGRIGLY
PANYVECVGA*

Coding Sequence Details

Insert Sequence Verified?:
Y
Verification Method:
Sequence Verification
Mutations:
None
Discrepancy:
None
5' Linker Sequence:
None
3' Linker Sequence:
None

Recommended Growth Condition:

Distributed in bacterial strain:
DH5-alpha T1 phage resistant
Antibiotic Selection:
Host Type:  bacterial    Marker:  kanamycin
Bacterial Selection Condition: 50ug/mL kanamycin
Growth Condition: Growth with the single antibiotic in LB at 37 degrees is recommended.
Comments: Conditions for Gateway-type vectors in recombined (with insert) form and other kanamycin resistant vectors.
Protein Expression Results:
ProteinExpressed: Not_Applicable
ProteinPurified: Not_Applicable
SolubleProtein: Not_Applicable
Find experimental protocols in GO.34568
Recommended expression in:
Not Applicable