44494526 (E. genome shotgun sequencing of the Sargasso Sea )
In pSGX3 (His-tagged bacterial expression vector)

Explanation of Terms

Gene:
Gene Symbol: 
Gene Name: 
Original Clone ID:
9373b1BCt4p1
Keyword:
full-length cds
Species:
Environmental genome shotgun sequencing of the Sargasso Sea
Type:
cDNA
Vector Name:
pSGX3
Format: 
CLOSED
Source:
New York SGX Research Center for Structural Genomics
Description:
None
Comments:
Linker not verified.
Mutation / Discrepancy:
No / Yes
Publications:
None
Authors:
PSI
New York SGX Research Center for Structural Genomics
EgCD00533621
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AVAILABLE
No restriction
Special MTA: PSI
Insert sequence: 1209nts
Open reading frame: 1 to 1209

AAAGAGTTTCTGATTAAGAAAATCGAGTTCACCTTATTCGAGTTGTGCATCCCGGATATC
GGCGCGGACCCAAGCGGTTTTGGGGTTTGGTATGAACCTGGACCCGGCACGCCGCAGAAA
CGTTTTGCCGTCCGCATTTTCACTGATGAAGGTCCAGTAGGGGTGTACGTTCCTCCGCGG
TCTAGAGCAACAGTCATAATGCCCGCTGCGATTGCCTTGGCACATTTTCTTATACGAAAG
CCGGCTCTCGAACGTGAGCGCCACTATCAAACCATGCGTCGCATTACGAAACATGTAGGA
GAAGCGGGCATCGGTGCCCTGGACATTGCACTGTGGGATTTAGGCGGTAAAATAAGTGGG
CAGCCAATCTCACAAATGTTGGGAGGCCACCGGCGTAAGCTTCCTTCCTACGCTTCGACT
ATTCCGGGTGACGAGCATCCCAAAGGCCTGAGCTCTAGTGAAGCGTATGCCGATTTCGCA
CAGCAGTGTCTCGAAATCGGTTACAAAGGGTATAAGATGCACGGATGGAAAGAGGGCAAT
CCGCAACGCGAATCAGAGATGATTAGAGCTGTGGCGCGACAGGTTGGTGGGTCCATGGAT
ATAATGTACGACGCCGCATGCCATCTGAAAACATTAACCGATGCGATCCGTGTCGGCCGC
GTGTGTGACGAACACGAATTACTGTGGTATGAGGACCCATATGCTGACGGTGGACTTACG
ATTCGGGGCCATCGTGCCCTGAAGGAAAAAGTACGCACCCCTGTTATGATAGGTGAGCAC
GTGCGTAACCCGGAAATCCATACTGAGCTTTTAGTCTCGGGGGCAACAGATTTTGGCCGC
GTTGATCCAGACTACGATGGTGGAATTACGGGCAGCTATAAAGCGGCTATGGCCGCAGAG
TCTCTGGGTTACGACGTAGAAGTGCACAGTTGCGGGCCGGCGATGAGACAATTGATGGCT
GCCCTTTCAATTTCCAATTATTACGAAATCAACCTGGTCCATCCCATTGGACCTAATCCG
TGGCAGTTACCAGTTTATCTGAACGGCTACTCGGATGAGCTCGATTGTATAGACCGAGAA
GGTATGGTGGATGTAAGCCAGTCTCCGGGGCTGGGCGTCGACTATGATTGGGAGTGGATC
GAAAAGAATAGTTTGGAACGTATTATTGTTGATAAAGCGAAAGAAGGGCATCATCACCAT
CACCACTGATCCGAATTCGAGCTCCGTCGAC

Protein sequence predicted based on nucleotide sequence

KEFLIKKIEFTLFELCIPDIGADPSGFGVWYEPGPGTPQKRFAVRIFTDEGPVGVYVPPR
SRATVIMPAAIALAHFLIRKPALERERHYQTMRRITKHVGEAGIGALDIALWDLGGKISG
QPISQMLGGHRRKLPSYASTIPGDEHPKGLSSSEAYADFAQQCLEIGYKGYKMHGWKEGN
PQRESEMIRAVARQVGGSMDIMYDAACHLKTLTDAIRVGRVCDEHELLWYEDPYADGGLT
IRGHRALKEKVRTPVMIGEHVRNPEIHTELLVSGATDFGRVDPDYDGGITGSYKAAMAAE
SLGYDVEVHSCGPAMRQLMAALSISNYYEINLVHPIGPNPWQLPVYLNGYSDELDCIDRE
GMVDVSQSPGLGVDYDWEWIEKNSLERIIVDKAKEGHHHHHH*

Coding Sequence Details

Insert Sequence Verified?:
Y
Verification Method:
Sequence Verification
Mutations:
None
Discrepancy:
a2c,K1T;a5t,E2V;
g43t,V15L;c679t,g681a,L227L;

t682c,L228L;a693g,E231E;
t696c,D232D;a807g,E269E;

c810t,L270L
                                                    

5' Linker Sequence:
None
3' Linker Sequence:
None

Recommended Growth Condition:

Distributed in bacterial strain:
DH5-alpha T1 phage resistant
Antibiotic Selection:
Host Type:  bacterial    Marker:  kanamycin
Bacterial Selection Condition: 50ug/mL kanamycin
Growth Condition: Growth with the single antibiotic in LB at 37 degrees is recommended.
Comments: Conditions for Gateway-type vectors in recombined (with insert) form and other kanamycin resistant vectors.
Protein Expression Results:
ProteinExpressed: Protein_Confirmed
ProteinPurified: Tested_Not_Purified
SolubleProtein: Tested_Not_Soluble
Find experimental protocols in NYSGXRC-9373b
Recommended expression in:
Not Applicable