Gmet_3143 (G. metallireducens GS-15 )
In pSGX3 (His-tagged bacterial expression vector)

Explanation of Terms

Gene:
Gene Symbol:  Gmet_3143
Gene Name:  resolvase-like serine recombinase
Original Clone ID:
11221f4BCt12p1
Keyword:
None
Species:
Geobacter metallireducens GS-15
Type:
cDNA
Vector Name:
pSGX3
Format: 
CLOSED
Source:
New York SGX Research Center for Structural Genomics
Description:
None
Comments:
Linker not verified.
Mutation / Discrepancy:
No / Yes
Publications:
None
Authors:
PSI
New York SGX Research Center for Structural Genomics
GmCD00533959
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AVAILABLE
No restriction
Special MTA: PSI
Insert sequence: 1890nts
Open reading frame: 42 to 1931

AATTTTGTTTAACTTTAGAAGGAGATATACATATGTCCCTTGAACGACCTCTTTGTATTT
CGTATATCCGGTTTTCAAAAGCAACACAAGCAGAGGGAAGCAGCTTGGAAAGACAGCTTC
AACTCTCCCGAACGTACGCAGAAGAACACGGGTTAGTGCTAGATGATAAACATTCCTATC
AAGATTTGGGTAAGTCCGCCTTCACGAGTGAACATGTCAAGGAAGGTGAATTTGGTGTAT
TTCTCGAATTTGTGAAGCAAGGACGGGTGCCTAAAGGATCGGTTCTTCTAGTCGAATCGC
TGGACCGATTGAGTCGAGAGAGTGTATTGACAGCCTTCAAGCAATTTCAAGAGATTTTGG
ACGCTGGCATCAAAATCGTTACTCTTAGTGACAAAATGGAGTACACAAAAGAAGGATTTG
AAGCGAATATCGGCAACATATTCATTTCCTTGGGTATTATGGCTCGTGCAAATGAAGAAA
GCTTGCGGAAATCAGAACGAATATGTGCGGCGTGGCGTCTGAAGCGCAAAAATCTTCACA
ATATAAAGTTGACAGCGAATTGTCCAGGGTGGCTTGAACTAACTCCTGACAAGAAAGATT
TTATACTGAAAGAAGACAGAGTCGAAATTGTAAAACAAATATTTGAATTATCTTTATCTG
GTCTTGGTGTAAAGGCAATATCAACCATATTAAATAAACAAAAAATAGAACCTTTTAGGT
CAAAAATAGGTTGGTATCAGACAACCGTCAGAAAAATCCTTTCCAATAGTGCTGTGATTG
GAGAGTTTCAGCCACATGTGTTTGGCTCAAAACATTCAAAACCGGTTCCGGACGGTGAAC
TAATCAAAGATTATTTTCCTCCTATTATAGACACCGATACATTCTATGCAGTACAAGCTA
AATTGAAAAACGGAGCACATAGACCTGGTAGAACTGCTAAAGTAGAAAATTTGTTTAGCG
GATATACAAGGTGTGGTTACTGTGGTTCACGTATGGATGTTGTTGCTAGGCATAACGTTG
GAGGTGAAGTAAGGTATCTGGTATGTGATAGTGCAAGACGCGGAGTCAAGTGTAGTTACA
TTTCTATGAAATGTAATGAGATCGAGAAAGCATTTTTTGAATATTGCAGAGAAATTGATA
TTAGATATGTGCTTAGAGCAGAACAACAGGCAGACCAGAATAGATTAAATGAACTGAGAA
AAAGCTTAACGGCAAGAAATGGCAAACTAATGAAGGTGCAAAAACAAATTGATATGTTGA
ATGTTTTTTTAGATGATATTACTGAAAGAGCAGAATTAGAGCATTACCACGAACAGATAA
AGAAAAAGTTAGAAGAAAAAACTAACCTATCTGAAGAGATAGAAAAATTGAGTAAGGGAA
TATGCATCGCAGAATATGATAATAATAATATAGATGTTAGAGTGCAAAATTTGCTTACTT
TGGATAAGGCACAGAATAAATCAGTGAAAGAAGATGACAAAATAATGTTTAGAATCAGAT
TGCGTGACGAGTTAAGACAAATAATAAGCCAAATATTAGTATACCCGAGAGGAAGCGTCT
TTAGTGATGAACAGATAGAACAGAAAGAAAGAGACTACGAAATTAAAATAAAAAGTGCAT
CTGATTCAGAAAAACACATTCTGAAGCATGAAAAAACCATTGATTTGGATGGAATGAGGC
AGTCACAGCAAAACACAAAAGATGATAGATTCTTCGTCGTCAGGTTCAAAAACGGTAACT
ACCGAACTATTAAATACGACAAGAAACAACAAGCGTACCGGGTAACTTCAGAATTAATTA
ACGATAAATTGAATTGGTGGATGGGGAATAAAGAAATGGCGCCAATATTTAGAGAAGAGG
AAGAATTACACCCATTCGAAACCGAATTTGAACTGGATTCAAATGAAGGGCATCATCACC
ATCACCACTGATCCGAATTCGAGC

Protein sequence predicted based on nucleotide sequence

ERPLCISYIRFSKATQAEGSSLERQLQLSRTYAEEHGLVLDDKHSYQDLGKSAFTSEHVK
EGEFGVFLEFVKQGRVPKGSVLLVESLDRLSRESVLTAFKQFQEILDAGIKIVTLSDKME
YTKEGFEANIGNIFISLGIMARANEESLRKSERICAAWRLKRKNLHNIKLTANCPGWLEL
TPDKKDFILKEDRVEIVKQIFELSLSGLGVKAISTILNKQKIEPFRSKIGWYQTTVRKIL
SNSAVIGEFQPHVFGSKHSKPVPDGELIKDYFPPIIDTDTFYAVQAKLKNGAHRPGRTAK
VENLFSGYTRCGYCGSRMDVVARHNVGGEVRYLVCDSARRGVKCSYISMKCNEIEKAFFE
YCREIDIRYVLRAEQQADQNRLNELRKSLTARNGKLMKVQKQIDMLNVFLDDITERAELE
HYHEQIKKKLEEKTNLSEEIEKLSKGICIAEYDNNNIDVRVQNLLTLDKAQNKSVKEDDK
IMFRIRLRDELRQIISQILVYPRGSVFSDEQIEQKERDYEIKIKSASDSEKHILKHEKTI
DLDGMRQSQQNTKDDRFFVVRFKNGNYRTIKYDKKQQAYRVTSELINDKLNWWMGNKEMA
PIFREEEELHPFETEFELDSNEGHHHHHH*

Coding Sequence Details

Insert Sequence Verified?:
Y
Verification Method:
Sequence Verification
Mutations:
None
Discrepancy:
a1302t,T434T
                                                    

5' Linker Sequence:
None
3' Linker Sequence:
None

Recommended Growth Condition:

Distributed in bacterial strain:
DH5-alpha T1 phage resistant
Antibiotic Selection:
Host Type:  bacterial    Marker:  kanamycin
Bacterial Selection Condition: 50ug/mL kanamycin
Growth Condition: Growth with the single antibiotic in LB at 37 degrees is recommended.
Comments: Conditions for Gateway-type vectors in recombined (with insert) form and other kanamycin resistant vectors.
Protein Expression Results:
ProteinPurified: Not_Tested
ProteinExpressed: Protein_Confirmed
SolubleProtein: Tested_Not_Soluble
Find experimental protocols in NYSGXRC-11221f
Recommended expression in:
Not Applicable